LOCUS       sequence-401255-        2483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_108 [TAmarelleT9]
FEATURES             Location/Qualifiers
     source          1..2483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(527..543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(527..543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(540..562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(715..1303)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(795..814)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1436..1530)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1551..2210)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2125..2144
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2211..2313)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2400..2422
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2414..2431
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2415..2431
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca gcttggaatc tccttctcgc ctctttcgct gaacacgaaa
      121 gcgaatccgt tcagcacaca ctttacgata tggcgcaaaa agtcctcggc tgcgtgccgg
      181 aagtgaaaga catccacctc accatgccca acaagcattg cctgctcgtg gacctgtccc
      241 gcttcggtca ggacaatccc aacgacgctt gagaccaccc caggatacat agattaccac
      301 aactccgagc ccttccacct agcgtgctaa ccacgcacgc tattgttgta tcccatataa
      361 tgcaatcgta gtctacctga ctgtacttag aaatgtggct tcgcctttgc ccacgcacct
      421 gatcgctcct cgtttgcttt taaggaccgg acgaaccaca gagcattaga aggctaggtg
      481 gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc
      541 ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat ccgacaatct
      601 ccaagacatt aggtggagtt cagttcggcg tatggcatat gtcgctggaa agaacatgtg
      661 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      721 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      781 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      841 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      901 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      961 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1021 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1081 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1141 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1201 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1261 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1321 ttctacgggg tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg
     1381 ggtagggggc ttcaaatcgt ccccccatac gatataagtt gttactagtg cttggattct
     1441 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     1501 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     1561 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     1621 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     1681 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     1741 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     1801 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1861 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1921 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1981 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     2041 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     2101 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     2161 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     2221 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     2281 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attgatacct gaaacaaaac
     2341 ccatcgtacg gccaaggaag tctccaataa ctgtgatcca ccacaagcgc cagggttttc
     2401 ccagtcacga cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa
     2461 ggaagtgcca ttccgcctga cct
//
