LOCUS       sequence-401256-        2388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_56 [TermC]
FEATURES             Location/Qualifiers
     source          1..2388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(432..448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(432..448)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(445..467)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(620..1208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(700..719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1341..1435)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1456..2115)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2030..2049
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2116..2218)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2305..2327
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2319..2336
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2320..2336
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca gcttgttaag ggatgcataa actgcatccc ttaacttgtt
      121 tttcgtgtgc ctattttttg tgaatcgatt atgtcttttg cgcagtcggc cgcttgagac
      181 caccccagga tacatagatt accacaactc cgagcccttc cacctagcgt gctaaccacg
      241 cacgctattg ttgtatccca tataatgcaa tcgtagtcta cctgactgta cttagaaatg
      301 tggcttcgcc tttgcccacg cacctgatcg ctcctcgttt gcttttaagg accggacgaa
      361 ccacagagca ttagaaggct aggtggaggc tcagtgatga taagtctgcg atggtggatg
      421 catgtgtcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcag agggcacaat
      481 cctattccgc gctatccgac aatctccaag acattaggtg gagttcagtt cggcgtatgg
      541 catatgtcgc tggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      601 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      661 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      721 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      781 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      841 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      901 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      961 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1021 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1081 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     1141 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1201 gatctcaaga agatcctttg atcttttcta cggggtctga cgctctattc aacaaagccg
     1261 ccgtcccgtc aagtcagcgt aaatgggtag ggggcttcaa atcgtccccc catacgatat
     1321 aagttgttac tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     1381 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     1441 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
     1501 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg
     1561 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
     1621 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
     1681 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
     1741 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
     1801 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
     1861 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag
     1921 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt
     1981 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg
     2041 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt
     2101 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac
     2161 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac
     2221 gtctcattga tacctgaaac aaaacccatc gtacggccaa ggaagtctcc aataactgtg
     2281 atccaccaca agcgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtcatg
     2341 cataatccgc acgcatctgg aataaggaag tgccattccg cctgacct
//
