LOCUS       sequence-401258-        2851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_47 [bar]
FEATURES             Location/Qualifiers
     source          1..2851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(895..911)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(895..911)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(908..930)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1083..1671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1163..1182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1804..1898)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1919..2578)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2493..2512
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2579..2681)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2768..2790
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2782..2799
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2783..2799
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca aatgtctcct gaaagaaggc ctgctgatat tagaagggct
      121 actgaggctg atatgcctgc tgtgtgcact attgtgaatc attacattga gacttctact
      181 gtgaatttca ggactgagcc tcaagaacca caagagtgga ctgatgatct tgtgaggctt
      241 agagagaggt atccttggct tgttgctgaa gtggatggtg aagtggctgg tattgcttat
      301 gctggtcctt ggaaggctag gaatgcttac gattggactg ctgagtctac tgtttacgtg
      361 tctcctaggc atcaaagaac tggtcttgga tctactcttt acactcatct tttgaagtct
      421 ttggaggctc agggtttcaa gtctgtggtt gctgtgattg gacttcctaa tgatccttct
      481 gtgaggatgc atgaggctct tggttatgct cctaggggaa tgcttagagc tgctggtttc
      541 aaacatggaa attggcatga tgtgggattc tggcagcttg atttctctct tcctgttcct
      601 cctagacctg ttcttcctgt gactgagatt tgagctttga gaccacccca ggatacatag
      661 attaccacaa ctccgagccc ttccacctag cgtgctaacc acgcacgcta ttgttgtatc
      721 ccatataatg caatcgtagt ctacctgact gtacttagaa atgtggcttc gcctttgccc
      781 acgcacctga tcgctcctcg tttgctttta aggaccggac gaaccacaga gcattagaag
      841 gctaggtgga ggctcagtga tgataagtct gcgatggtgg atgcatgtgt catggtcata
      901 gctgtttcct gtgtgaaatt gttatccgct cagagggcac aatcctattc cgcgctatcc
      961 gacaatctcc aagacattag gtggagttca gttcggcgta tggcatatgt cgctggaaag
     1021 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1081 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1141 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1201 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1261 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1321 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1381 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1441 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1501 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1561 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1621 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1681 ttgatctttt ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag
     1741 cgtaaatggg tagggggctt caaatcgtcc ccccatacga tataagttgt tactagtgct
     1801 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     1861 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     1921 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     1981 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     2041 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     2101 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     2161 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     2221 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     2281 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     2341 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     2401 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     2461 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     2521 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     2581 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     2641 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tgatacctga
     2701 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
     2761 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
     2821 tggaataagg aagtgccatt ccgcctgacc t
//
