LOCUS       sequence-401260-        3070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_49 [tk101]
FEATURES             Location/Qualifiers
     source          1..3070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1114..1130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1114..1130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1127..1149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1302..1890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1382..1401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2023..2117)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2138..2797)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2712..2731
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2798..2900)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2987..3009
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3001..3018
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3002..3018
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca aatgagaata gtgaatggac caataataat gactagagaa
      121 gaaagaatga agattgttca tgaaattaag gaacgaatat tggataaata tggggatgat
      181 gttaaggcta ttggtgttta tggctctctt ggtcgtcaga ctgatgggcc ctattcggat
      241 attgagatga tgtgtgtcat gtcaacagag gaagcagagt tcagccatga atggacaacc
      301 ggtgagtgga aggtggaagt gaatttttat agcgaagaga ttctactaga ttatgcatct
      361 caggtggaat cagattggcc gcttacacat ggtcaatttt tctctatttt gccgatttat
      421 gattcaggtg gatacttaga gaaagtgtat caaactgcta aatcggtaga agcccaaaag
      481 ttccacgatg cgatttgtgc ccttatcgta gaagaactgt ttgaatatgc aggcaaatgg
      541 cgtaatattc gtgtgcaagg accgacaaca tttctaccat ccttgactgt acaggtagca
      601 atggcaggtg ccatgttgat tggtctgcat catcgcatct gttatacgac gagcgcttcg
      661 gtcttaactg aagcagttaa gcaatcagat cttccttcag gttatgacca tctgtgccag
      721 ttcgtaatgt ctggtcaact ttccgactct gagaaacttc tggaatcgct agagaatttc
      781 tggaatggga ttcaggagtg gacagaacga cacggatata tagtggatgt gtcaaaacgc
      841 ataccatttt gagctttgag accaccccag gatacataga ttaccacaac tccgagccct
      901 tccacctagc gtgctaacca cgcacgctat tgttgtatcc catataatgc aatcgtagtc
      961 tacctgactg tacttagaaa tgtggcttcg cctttgccca cgcacctgat cgctcctcgt
     1021 ttgcttttaa ggaccggacg aaccacagag cattagaagg ctaggtggag gctcagtgat
     1081 gataagtctg cgatggtgga tgcatgtgtc atggtcatag ctgtttcctg tgtgaaattg
     1141 ttatccgctc agagggcaca atcctattcc gcgctatccg acaatctcca agacattagg
     1201 tggagttcag ttcggcgtat ggcatatgtc gctggaaaga acatgtgagc aaaaggccag
     1261 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1321 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1381 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1441 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1501 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1561 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1621 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1681 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1741 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1801 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1861 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1921 gacgctctat tcaacaaagc cgccgtcccg tcaagtcagc gtaaatgggt agggggcttc
     1981 aaatcgtccc cccatacgat ataagttgtt actagtgctt ggattctcac caataaaaaa
     2041 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     2101 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     2161 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     2221 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     2281 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     2341 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     2401 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     2461 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     2521 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     2581 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     2641 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     2701 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     2761 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     2821 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     2881 aacctcttac gtgccgatca acgtctcatt gatacctgaa acaaaaccca tcgtacggcc
     2941 aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca gtcacgacgt
     3001 tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga agtgccattc
     3061 cgcctgacct
//
