LOCUS       sequence-401262-        2337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_97 [J23108]
FEATURES             Location/Qualifiers
     source          1..2337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..119
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     primer_bind     complement(381..397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(381..397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(394..416)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(569..1157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(649..668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1290..1384)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1405..2064)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1979..1998
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2065..2167)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2254..2276
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2268..2285
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2269..2285
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca ggagctgaca gctagctcag tcctaggtat aatgctagct
      121 acttgagacc accccaggat acatagatta ccacaactcc gagcccttcc acctagcgtg
      181 ctaaccacgc acgctattgt tgtatcccat ataatgcaat cgtagtctac ctgactgtac
      241 ttagaaatgt ggcttcgcct ttgcccacgc acctgatcgc tcctcgtttg cttttaagga
      301 ccggacgaac cacagagcat tagaaggcta ggtggaggct cagtgatgat aagtctgcga
      361 tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaga
      421 gggcacaatc ctattccgcg ctatccgaca atctccaaga cattaggtgg agttcagttc
      481 ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctctattca
     1201 acaaagccgc cgtcccgtca agtcagcgta aatgggtagg gggcttcaaa tcgtcccccc
     1261 atacgatata agttgttact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     1321 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     1381 agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg
     1441 taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat
     1501 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg
     1561 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     1621 attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     1681 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta
     1741 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     1801 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc
     1861 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     1921 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     1981 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     2041 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     2101 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg
     2161 ccgatcaacg tctcattgat acctgaaaca aaacccatcg tacggccaag gaagtctcca
     2221 ataactgtga tccaccacaa gcgccagggt tttcccagtc acgacgttgt aaaacgacgg
     2281 ccagtcatgc ataatccgca cgcatctgga ataaggaagt gccattccgc ctgacct
//
