LOCUS       sequence-401264-        2754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_54 [PermC]
FEATURES             Location/Qualifiers
     source          1..2754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(798..814)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(798..814)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(811..833)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(986..1574)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1066..1085)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1707..1801)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1822..2481)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2396..2415
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2482..2584)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2671..2693
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2685..2702
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2686..2702
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca ggaggatcct ttaactctgg caaccctcaa aattgaatga
      121 gacatgctac acctccggat aataaatata tataaacgta tatagatttc ataaagtcta
      181 acacactaga cttatttact tcgtaattaa gtcgttaaac cgtgtgctct acgaccaaaa
      241 ctataaaacc tttaagaact ttcttttttt acaagaaaaa agaaattaga taaatctctc
      301 atatctttta ttcaataatc gcatccgatt gcagtataaa tttaacgatc actcatcatg
      361 ttcatattta tcagaggtcg tgctataatt atactaattt tataaggagg aaaaaatatg
      421 ggcattttta gtatttttgt aatcagcaca gttcattatc aaccaaacaa aaaataagtg
      481 gttataatga atcgttaata agcaaaattc atataaccaa attaaagagg gttataaatg
      541 tgagaccacc ccaggataca tagattacca caactccgag cccttccacc tagcgtgcta
      601 accacgcacg ctattgttgt atcccatata atgcaatcgt agtctacctg actgtactta
      661 gaaatgtggc ttcgcctttg cccacgcacc tgatcgctcc tcgtttgctt ttaaggaccg
      721 gacgaaccac agagcattag aaggctaggt ggaggctcag tgatgataag tctgcgatgg
      781 tggatgcatg tgtcatggtc atagctgttt cctgtgtgaa attgttatcc gctcagaggg
      841 cacaatccta ttccgcgcta tccgacaatc tccaagacat taggtggagt tcagttcggc
      901 gtatggcata tgtcgctgga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      961 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1021 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1081 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1141 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1201 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1261 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1321 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1381 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1441 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1501 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1561 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct ctattcaaca
     1621 aagccgccgt cccgtcaagt cagcgtaaat gggtaggggg cttcaaatcg tccccccata
     1681 cgatataagt tgttactagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     1741 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     1801 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa
     1861 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     1921 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     1981 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     2041 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     2101 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     2161 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     2221 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt
     2281 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     2341 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     2401 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     2461 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa
     2521 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg
     2581 atcaacgtct cattgatacc tgaaacaaaa cccatcgtac ggccaaggaa gtctccaata
     2641 actgtgatcc accacaagcg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     2701 gtcatgcata atccgcacgc atctggaata aggaagtgcc attccgcctg acct
//
