LOCUS       sequence-401266-        2773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_40 [dfrA1]
FEATURES             Location/Qualifiers
     source          1..2773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(817..833)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(817..833)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(830..852)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1005..1593)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1085..1104)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1726..1820)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1841..2500)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2415..2434
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2501..2603)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2690..2712
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2704..2721
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2705..2721
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca aatgaaacta tcactaatgg tagctatatc gaagaatgga
      121 gttatcggga atggccctga tattccttgg agtgccaaag gtgaacagct cctgtttaaa
      181 gctattacct ataaccaatg gctgttggtt ggacgcaaga cttttgaatc aatgggagca
      241 ttacccaacc gaaagtatgc ggtcgtaaca cgttcaagtt ttacatctga caatgaggac
      301 gtattgatct ttccatcaat taaagatgct ttaaccaacc taaagaaaat aacggatcat
      361 gtcattgttt caggtggtgg ggagatatac aaaagcctga tcgatcaagt agatacacta
      421 catatatcta caatagacat cgagccggaa ggtgatgttt actttcctga aatccccagc
      481 aattttaggc cagtttttac ccaagacttc gcctctaaca taaattatag ttaccaaatc
      541 tggcaaaagg gttaagcttt gagaccaccc caggatacat agattaccac aactccgagc
      601 ccttccacct agcgtgctaa ccacgcacgc tattgttgta tcccatataa tgcaatcgta
      661 gtctacctga ctgtacttag aaatgtggct tcgcctttgc ccacgcacct gatcgctcct
      721 cgtttgcttt taaggaccgg acgaaccaca gagcattaga aggctaggtg gaggctcagt
      781 gatgataagt ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc ctgtgtgaaa
      841 ttgttatccg ctcagagggc acaatcctat tccgcgctat ccgacaatct ccaagacatt
      901 aggtggagtt cagttcggcg tatggcatat gtcgctggaa agaacatgtg agcaaaaggc
      961 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1021 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1081 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1141 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1201 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1261 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1321 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1381 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1441 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1501 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1561 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1621 tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc
     1681 ttcaaatcgt ccccccatac gatataagtt gttactagtg cttggattct caccaataaa
     1741 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1801 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1861 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1921 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1981 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     2041 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     2101 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     2161 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     2221 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     2281 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     2341 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     2401 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2461 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     2521 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     2581 tggaacctct tacgtgccga tcaacgtctc attgatacct gaaacaaaac ccatcgtacg
     2641 gccaaggaag tctccaataa ctgtgatcca ccacaagcgc cagggttttc ccagtcacga
     2701 cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa ggaagtgcca
     2761 ttccgcctga cct
//
