LOCUS       sequence-401269-        2504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_103 [tAgTEF1]
FEATURES             Location/Qualifiers
     source          1..2504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      89..286
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(548..564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(548..564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(561..583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(736..1324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(816..835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1457..1551)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1572..2231)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2146..2165
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2232..2334)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2421..2443
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2435..2452
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2436..2452
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca gcttagtaac tgacaataaa aagattcttg ttttcaagaa
      121 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt
      181 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa
      241 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgcgct tgagaccacc
      301 ccaggataca tagattacca caactccgag cccttccacc tagcgtgcta accacgcacg
      361 ctattgttgt atcccatata atgcaatcgt agtctacctg actgtactta gaaatgtggc
      421 ttcgcctttg cccacgcacc tgatcgctcc tcgtttgctt ttaaggaccg gacgaaccac
      481 agagcattag aaggctaggt ggaggctcag tgatgataag tctgcgatgg tggatgcatg
      541 tgtcatggtc atagctgttt cctgtgtgaa attgttatcc gctcagaggg cacaatccta
      601 ttccgcgcta tccgacaatc tccaagacat taggtggagt tcagttcggc gtatggcata
      661 tgtcgctgga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      721 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      781 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      841 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      901 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      961 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1021 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1081 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1141 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1201 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1261 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1321 tcaagaagat cctttgatct tttctacggg gtctgacgct ctattcaaca aagccgccgt
     1381 cccgtcaagt cagcgtaaat gggtaggggg cttcaaatcg tccccccata cgatataagt
     1441 tgttactagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga
     1501 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     1561 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     1621 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     1681 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     1741 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
     1801 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     1861 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     1921 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     1981 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
     2041 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     2101 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     2161 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     2221 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     2281 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct
     2341 cattgatacc tgaaacaaaa cccatcgtac ggccaaggaa gtctccaata actgtgatcc
     2401 accacaagcg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtcatgcata
     2461 atccgcacgc atctggaata aggaagtgcc attccgcctg acct
//
