LOCUS       sequence-401270-        2499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_34 [PaadA]
FEATURES             Location/Qualifiers
     source          1..2499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(543..559)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(543..559)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(556..578)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(731..1319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(811..830)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1452..1546)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1567..2226)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2141..2160
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2227..2329)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2416..2438
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2430..2447
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2431..2447
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca ggaggaacct tgaccgaacg cagcggtggt aacggcgcag
      121 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat
      181 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg
      241 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat caatgtgaga ccaccccagg
      301 atacatagat taccacaact ccgagccctt ccacctagcg tgctaaccac gcacgctatt
      361 gttgtatccc atataatgca atcgtagtct acctgactgt acttagaaat gtggcttcgc
      421 ctttgcccac gcacctgatc gctcctcgtt tgcttttaag gaccggacga accacagagc
      481 attagaaggc taggtggagg ctcagtgatg ataagtctgc gatggtggat gcatgtgtca
      541 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca gagggcacaa tcctattccg
      601 cgctatccga caatctccaa gacattaggt ggagttcagt tcggcgtatg gcatatgtcg
      661 ctggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1141 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1321 aagatccttt gatcttttct acggggtctg acgctctatt caacaaagcc gccgtcccgt
     1381 caagtcagcg taaatgggta gggggcttca aatcgtcccc ccatacgata taagttgtta
     1441 ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
     1501 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag cgagctcgat
     1561 atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
     1621 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
     1681 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
     1741 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
     1801 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
     1861 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
     1921 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
     1981 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag
     2041 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     2101 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     2161 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     2221 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     2281 tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattg
     2341 atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt gatccaccac
     2401 aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat gcataatccg
     2461 cacgcatctg gaataaggaa gtgccattcc gcctgacct
//
