LOCUS       sequence-401278-        2427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_100 [Pbs]
FEATURES             Location/Qualifiers
     source          1..2427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(471..487)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(471..487)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(484..506)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(659..1247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(739..758)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1380..1474)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1495..2154)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2069..2088
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2155..2257)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2344..2366
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2358..2375
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2359..2375
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca ggagcctcct tgacactgaa tttagcatgt gatataatta
      121 acttaatatt ctacccaagc ttataaaaga gcactgttgg gcgtgagtgg aggcgccgga
      181 aaaaagcatc gaaaaaagga ggaaaaaaaa atgtgagacc accccaggat acatagatta
      241 ccacaactcc gagcccttcc acctagcgtg ctaaccacgc acgctattgt tgtatcccat
      301 ataatgcaat cgtagtctac ctgactgtac ttagaaatgt ggcttcgcct ttgcccacgc
      361 acctgatcgc tcctcgtttg cttttaagga ccggacgaac cacagagcat tagaaggcta
      421 ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg
      481 tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca
      541 atctccaaga cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca
      601 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      661 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      721 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      781 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      841 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      901 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      961 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1021 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1081 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1141 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1201 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1261 tcttttctac ggggtctgac gctctattca acaaagccgc cgtcccgtca agtcagcgta
     1321 aatgggtagg gggcttcaaa tcgtcccccc atacgatata agttgttact agtgcttgga
     1381 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     1441 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     1501 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     1561 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     1621 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1681 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     1741 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     1801 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     1861 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     1921 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     1981 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     2041 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     2101 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     2161 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     2221 attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattgat acctgaaaca
     2281 aaacccatcg tacggccaag gaagtctcca ataactgtga tccaccacaa gcgccagggt
     2341 tttcccagtc acgacgttgt aaaacgacgg ccagtcatgc ataatccgca cgcatctgga
     2401 ataaggaagt gccattccgc ctgacct
//
