LOCUS       sequence-401281-        2358 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_53 [TcatA]
FEATURES             Location/Qualifiers
     source          1..2358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(402..418)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(402..418)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(415..437)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(590..1178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(670..689)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1311..1405)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1426..2085)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2000..2019
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2086..2188)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2275..2297
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2289..2306
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2290..2306
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca gctttatgag ataatgccga ctgtactttt tacagtcggt
      121 tttctaatgt cactaacctg cgcttgagac caccccagga tacatagatt accacaactc
      181 cgagcccttc cacctagcgt gctaaccacg cacgctattg ttgtatccca tataatgcaa
      241 tcgtagtcta cctgactgta cttagaaatg tggcttcgcc tttgcccacg cacctgatcg
      301 ctcctcgttt gcttttaagg accggacgaa ccacagagca ttagaaggct aggtggaggc
      361 tcagtgatga taagtctgcg atggtggatg catgtgtcat ggtcatagct gtttcctgtg
      421 tgaaattgtt atccgctcag agggcacaat cctattccgc gctatccgac aatctccaag
      481 acattaggtg gagttcagtt cggcgtatgg catatgtcgc tggaaagaac atgtgagcaa
      541 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      601 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      661 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      721 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      781 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      841 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      901 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      961 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1021 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1081 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1141 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1201 cggggtctga cgctctattc aacaaagccg ccgtcccgtc aagtcagcgt aaatgggtag
     1261 ggggcttcaa atcgtccccc catacgatat aagttgttac tagtgcttgg attctcacca
     1321 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1381 attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg
     1441 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
     1501 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
     1561 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
     1621 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     1681 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1741 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     1801 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1861 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1921 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     1981 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     2041 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     2101 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     2161 aaagttggaa cctcttacgt gccgatcaac gtctcattga tacctgaaac aaaacccatc
     2221 gtacggccaa ggaagtctcc aataactgtg atccaccaca agcgccaggg ttttcccagt
     2281 cacgacgttg taaaacgacg gccagtcatg cataatccgc acgcatctgg aataaggaag
     2341 tgccattccg cctgacct
//
