LOCUS       sequence-401282-        2346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_27 [Tfd gene VIII]
FEATURES             Location/Qualifiers
     source          1..2346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      88..127
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     primer_bind     complement(390..406)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(390..406)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(403..425)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(578..1166)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(658..677)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1299..1393)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1414..2073)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1988..2007
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2074..2176)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2263..2285
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2277..2294
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2278..2294
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca gcttaccgat acaattaaag gctccttttg gagccttttt
      121 ttttggaccg cttgagacca ccccaggata catagattac cacaactccg agcccttcca
      181 cctagcgtgc taaccacgca cgctattgtt gtatcccata taatgcaatc gtagtctacc
      241 tgactgtact tagaaatgtg gcttcgcctt tgcccacgca cctgatcgct cctcgtttgc
      301 ttttaaggac cggacgaacc acagagcatt agaaggctag gtggaggctc agtgatgata
      361 agtctgcgat ggtggatgca tgtgtcatgg tcatagctgt ttcctgtgtg aaattgttat
      421 ccgctcagag ggcacaatcc tattccgcgc tatccgacaa tctccaagac attaggtgga
      481 gttcagttcg gcgtatggca tatgtcgctg gaaagaacat gtgagcaaaa ggccagcaaa
      541 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      601 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      661 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      721 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      781 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      841 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      901 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      961 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1021 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1081 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1141 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1201 ctctattcaa caaagccgcc gtcccgtcaa gtcagcgtaa atgggtaggg ggcttcaaat
     1261 cgtcccccca tacgatataa gttgttacta gtgcttggat tctcaccaat aaaaaacgcc
     1321 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     1381 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
     1441 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     1501 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     1561 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1621 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     1681 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1741 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1801 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1861 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1921 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1981 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     2041 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     2101 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     2161 tcttacgtgc cgatcaacgt ctcattgata cctgaaacaa aacccatcgt acggccaagg
     2221 aagtctccaa taactgtgat ccaccacaag cgccagggtt ttcccagtca cgacgttgta
     2281 aaacgacggc cagtcatgca taatccgcac gcatctggaa taaggaagtg ccattccgcc
     2341 tgacct
//
