LOCUS       sequence-401288-        3109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_43 [tsr]
FEATURES             Location/Qualifiers
     source          1..3109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1153..1169)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1153..1169)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1166..1188)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1341..1929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1421..1440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2062..2156)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2177..2836)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2751..2770
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2837..2939)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     3026..3048
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3040..3057
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3041..3057
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca aatgactgag ttggacacca tcgcaaatcc gtccgatccc
      121 gcggtgcagc ggatcatcga tgtcaccaag ccgtcgcgat ccaacataaa gacaacgttg
      181 atcgaggacg tcgagcccct catgcacagc atcgcggccg gggtggagtt catcgaggtc
      241 tacggcagcg acagcagtcc ttttccatct gagttgctgg atctgtgcgg gcggcagaac
      301 ataccggtcc gcctcatcga ctcctcgatc gtcaaccagt tgttcaaggg ggagcggaag
      361 gccaagacat tcggcatcgc ccgcgtccct cgcccggcca ggttcggcga tatcgcgagc
      421 cggcgtgggg acgtcgtcgt tctcgacggg gtgaagatcg tcgggaacat cggcgcgata
      481 gtacgcacgt cgctcgcgct cggagcgtcg gggatcatcc tggtcgacag tgacatcacc
      541 agcatcgcgg accggcgtct ccaaagggcc agccgaggtt acgtcttctc ccttcccgtc
      601 gttctctccg gtcgcgagga ggccatcgcc ttcattcggg acagcggtat gcagctgatg
      661 acgctcaagg cggatggcga catttccgtg aaggaactcg gggacaatcc ggatcggctg
      721 gccttgctgt tcggcagcga aaagggtggg ccttccgacc tgttcgagga ggcgtcttcc
      781 gcctcggttt ccatccccat gatgagccag accgagtctc tcaacgtttc cgtttccctc
      841 ggaatcgcgc tgcacgagag gatcgacagg aatctcgcgg ccaaccgata agctttgaga
      901 ccaccccagg atacatagat taccacaact ccgagccctt ccacctagcg tgctaaccac
      961 gcacgctatt gttgtatccc atataatgca atcgtagtct acctgactgt acttagaaat
     1021 gtggcttcgc ctttgcccac gcacctgatc gctcctcgtt tgcttttaag gaccggacga
     1081 accacagagc attagaaggc taggtggagg ctcagtgatg ataagtctgc gatggtggat
     1141 gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca gagggcacaa
     1201 tcctattccg cgctatccga caatctccaa gacattaggt ggagttcagt tcggcgtatg
     1261 gcatatgtcg ctggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1321 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1381 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1441 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1501 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1561 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1621 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1681 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1741 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1801 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1861 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1921 ggatctcaag aagatccttt gatcttttct acggggtctg acgctctatt caacaaagcc
     1981 gccgtcccgt caagtcagcg taaatgggta gggggcttca aatcgtcccc ccatacgata
     2041 taagttgtta ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     2101 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     2161 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     2221 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     2281 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     2341 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     2401 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     2461 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     2521 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     2581 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     2641 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     2701 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     2761 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     2821 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     2881 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     2941 cgtctcattg atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
     3001 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
     3061 gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgacct
//
