LOCUS       sequence-401291-        2635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_20 [panARS-opt ORI]
FEATURES             Location/Qualifiers
     source          1..2635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(494..588)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(609..1268)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1183..1202
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1269..1371)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1458..1480
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1472..1489
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1473..1489
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2220..2236)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2220..2236)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2233..2255)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(join(2408..2635,1..361))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2488..2507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
       61 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      181 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      361 agaagatcct ttgatctttt ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc
      421 gtcaagtcag cgtaaatggg tagggggctt caaatcgtcc ccccatacga tataagttgt
      481 tactagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
      541 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
      601 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
      661 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
      721 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
      781 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
      841 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
      901 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
      961 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     1021 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     1081 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     1141 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     1201 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     1261 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     1321 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     1381 tgatacctga aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc
     1441 acaagcgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc
     1501 cgcacgcatc tggaataagg aagtgccatt ccgcctgacc ttagcgtgcg aacagcacgc
     1561 tattgttgta tcattactcg catccattct caggctgtct cgtctcgtct ccgaggtctc
     1621 cggagtcaac atctttggat aatatcagaa tgagaaagaa cagatacgca gtacgttttt
     1681 tggtgagctc tttgcacttc tttagttctt tccatcaata tcagtttttt aaacttttag
     1741 gactaaaagt gatgtttaac ttcaaaatgt ttaaaatttt gttcttcccg acgttcatta
     1801 agaatactaa tacactttaa taattatttt aatattttgt tctaaataat gacttttaat
     1861 taaaaaagat aaaatataaa aacatcataa taactcacca gaggttaaga acaaaaaaac
     1921 aaattagata tctgctaatc caatatagtt aaatcaatct ttccttggta taatgggtat
     1981 attacatata tttcaaggac cgacactcct accaaatatc taaaatttac catattaaca
     2041 taacatgtat ataaacgtca aatcataatc agcactacgc tcgagaccac cccaggatac
     2101 atagattacc acaactccga gcccttccac ctagcgtgct aaccacgcac gctattgttg
     2161 tataggctag gtggaggctc agtgatgata agtctgcgat ggtggatgca tgtgtcatgg
     2221 tcatagctgt ttcctgtgtg aaattgttat ccgctcagag ggcacaatcc tattccgcgc
     2281 tatccgacaa tctccaagac attaggtgga gttcagttcg gcgtatggca tatgtcgctg
     2341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgta
//
