LOCUS       sequence-401294-       11212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitous zebrafish expression of mKate2 fused to the actin binding
            lifeact sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11212)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 11212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: actb2-lifeact-mKate2
FEATURES             Location/Qualifiers
     source          1..11212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(54..75)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(424..435,436..598)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     971..987
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1007..1027
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6334..6358
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6359..6368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6365..6415
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     protein_bind    complement(6417..6441)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6443..7138
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     polyA_signal    7937..8018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8019..8039)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8047..8063)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    8079..8213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8103..8122)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(8652..8839,8840..8851)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8885..8901)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8885..8901)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8938..8958)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8938..8956)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8977..8993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8990..9012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9001..9017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9025..9031,9032..9049,9050..9055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9070..9091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9208..9225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9379..9967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9459..9478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10138..10929,10930..10998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10761..10780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10999..11103)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
       61 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
      121 atcagggcga tggcccacgc tggctaagaa ctcatcagcc tccccggtcc atctacccac
      181 gtaccaatgc accaattggc cacaatgacg gctactacat ggtgccattc cttcctcttt
      241 ataggaatgg agactacctc ctgtccaaca atgctcttgg atacgagtac gcctacctgt
      301 tggacccagg tcattgcaca acaccagaaa tgccctctga tctgcaaaag acgtgaatat
      361 ctgttcagac acccatatcc actctgttcc acacaggtca gaggtttgtc caggagttct
      421 tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt gaaagtacaa gtacttaggg
      481 aaaattttac tcaattaaaa gtaaaagtat ctggctagaa tcttacttga gtaaaagtaa
      541 aaaagtactc cattaaaatt gtacttgagt attaaggaag taaaagtaaa agcaagaaag
      601 aaaactagag attcttgttt aagcttttaa tctcaaaaaa cattaaatga aatgcataca
      661 aggttttatc ctgctttaga actgtttgta tttaattatc aaactataag acagacaatc
      721 taatgccagt acacgctact caaagttgta aaacctcaga tttaacttca gtagaagctg
      781 attctcaaaa ttgttagtgt caagcctagc tcttttgggg ctgaaaagca atcctgcagt
      841 gctgaaaagc ctctcacagg cagccgatgc gggaagaggt gtattagtct tgatagagag
      901 gctgcaaata gcaggaaacg tgagcagaga ctccctggtg tctgaaacac aggccagatg
      961 ggccctcgag caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga
     1021 aaagttgaaa attccagttt gaagaaactt ttcaagactc accttttcac tgttggtctt
     1081 aatttaggtt gattattttt gtcaatttaa taattacatt gtattgttca tattttattg
     1141 tcttattgtg gtgtttttac agctttttta ttgttctgct ttgttactgt ttgccttgta
     1201 tgcattgtag cgctttgggt ctgagaaaag cgcactataa ataaactgta ttattattat
     1261 tagggatgga catcacggtg gcgcgatggg tggcacaatt acctcacagc aagtaggttg
     1321 ctgtttagag ccccggctgg atcagttggc agttctgtgt ggagtttgca tgttctccct
     1381 gttcaagtag gtttcctccc ggtgctctgg tttcccctca caagtccaaa gacatgatgt
     1441 ataggtgaat ggggtaagct aaattgacca tagtgagtgt gtgtgtgtgt gtgaatgaga
     1501 gtgtataggt gtttcccagt gttaggttgc aactggaagg acatctgctg tgtaaaacat
     1561 atgcaggata agttggcggt tcattcccct atggtgaccc ctggttaatt aaaggactaa
     1621 gttgaaaatg aatgaatgaa tgaatgatta gagataaacc gataccactt ttttacaaac
     1681 cgatatgagt acaagtataa tagtttctgt tactcgacaa tactgagtac cgattctgat
     1741 acttcttgga tttggtttca atgtaattaa tttgaaagaa ggttttattt cttgcctgtc
     1801 gcaaggatga acacaaaaat ctttaccaga aggatatttt aagctaagaa tatacgcacg
     1861 ttgttgacaa ccctgtatcg attaccgatt aaacgtggac atttaaagga taagttatgc
     1921 ttgcgccatc acaactttac acattacatg atcaaagatc atattgaata ttcggtttat
     1981 tcgtcagaag cctatagatt tgttaaaagt gtattttata aacggatggt ttataaagac
     2041 taagctatta atatatcaga cattcattaa tacatcaaga catcgatgtc aggtaggaca
     2101 acaaatcaca tccatgtaat gaaatcttgt tgagtggtat tggtgcgtgg tttcagcatt
     2161 tcatgtgcaa tacgagtaag aatattttaa ccaagtatcg acccaaaacc tgatactggt
     2221 atcggtgcat cccttattat tattattatc aaaaacacac ttgttttatt tatgtgggtg
     2281 ccaaacaaca aactatgaaa tgcagcatga cacgcccaaa aagtgttcct ttgagaacaa
     2341 cttcataaat atgtttctta tcattatgca tgaatggtgt actgggagag ctttggtatc
     2401 agaattgaga taagagactt tggcattcta aaggacaatg acattatcac actgacagtc
     2461 aacaacactt tattgctctg gaaatcttat gtcatggggc aaagctgagg ttcattagga
     2521 cagtacctta tttagagatg aatgggaagg ctgtagtggg ctgctatcag ggaagggtgg
     2581 tggtcctcgg gggaactatg gactcacaaa agcacatctg ctgtaacaag atcccctgtg
     2641 caatctacaa aagttacaaa gagaagcatg gttggttaaa aaatagaatt gactggatat
     2701 gctgaatgca ttgtttgttg tgtgttacat cacattaacc acagattggt gctaaatatt
     2761 tgcttatctt ataaaaacct gtgtgtttat aaaggggggt gtggatggat gcttggcatt
     2821 ctttgaaaag atgttggggg atggaaggag gagaccttat tgatgagtat ccagtttgca
     2881 gataacaagt ttttagacat tcatactatg atgctgttta tgtctgtttt ttctcatttt
     2941 ttctatacac aaacagctat aaaacattca cttttatatc tgatgctgta tattaaattt
     3001 cgttgtattt attctgtttg ctctgttggg tcatagctag ggccagacag aatctgctga
     3061 cattttttgc tttttctgca gagaattttg taaacaatct gtgatttatg cgagattttt
     3121 tcgggagtat cgtaactaaa aacttactat atgaaataaa aaaaaataat acctttttaa
     3181 cttttaataa atatatacaa tgcaagtcca cctagatcca attatttggt cgacaaagca
     3241 agtctctcac ataatatatc ttctaaaaga cagaaaacat tactttcaga ccatattgta
     3301 atcaaatgaa cactttcata ttagtcaata atattacgga aatgtattta aaaactgaat
     3361 aaacatatat ttacaccttt gtctttattt ctagagtgct tttacaatgt agattgtgtc
     3421 aaagcagctt aacttagagg attagtatag taaattgaaa ctgtgtcagt ccatttttta
     3481 agagtttaag ttcagttcag tttagttcag tgtgagttaa tttttactgc tgagagtcca
     3541 aacactaaag agcaaatcca aatacacaag tcaataaata aactcaatga tgggctaaag
     3601 atctggggaa atctgcagat ttctgtgcgc acagattctg tgtgggccta gtcattacct
     3661 atactcaacg tttactctga gaaacttcca tattttagag gtaaaatgct cagaattgta
     3721 aaaagtcttg tcaaaatact tggaatttgc caaaaacaaa ataaaagtca catcacctca
     3781 ttgtccttgg tatcagcaag cacttttaac acaatcagct gtttttgttt ataactccca
     3841 ctgaaggcca tgtgcattac tcccaagtta cacagtaaaa agtcacatgt agtttatcat
     3901 gatcactgct gctcaagcac agctttatct gttattcagt gtggcaatat ctgaaatgtg
     3961 tgcaaaatat tatttattgt agctgatcac caacactttc agtgtttttt ttttctactt
     4021 ttaatgagaa ttatgttctt aggatttatc cttataaacc tctttaaaaa gaaatgttat
     4081 gtctgcatag acacgacatg aaattatgaa cagaaaattt aactatttaa gatcaagact
     4141 tgacatataa catttctaat gaacattaaa cctgaggtag gcccacttgc aaaaatgtaa
     4201 ctgctatcac tcactttata catcattgtg taacttgtac acacaaaaat agtgaagcaa
     4261 tgtggcatgg aatgcaggtc ccattcattc aaagttatta aagcactttc ttaaagtgca
     4321 ttactactga gctaagacca gctagttgct catacaacat actacaagta gtgtcatcct
     4381 aatttggata aaaaaaatgt ccattttata catggaaaag tactcattag atcccccatt
     4441 gtttgtatta cggtatttcg tgaacacaag aggtaaatga cctacagagc tgctgttgtg
     4501 ttagattgaa aacacaacac aggatcatgg aggcatccat cattatgacg cattctgcca
     4561 cttgtaactc tgcacagact ttttgaaaag tttaattgag tcttggcgtt gtcgcagaaa
     4621 aatacgaaaa attctgtgtt aaaatttgca aataaaaatt ccagttttat aggaaatcgc
     4681 catggccttg ttctttaacc agttaagctt ccccttcttt cactctcaag ttgcaagaag
     4741 caagtgtagc aatgtgcacg cgacagccgg gtgtgtgacg ctggaccaat cagagcacag
     4801 agctccgaaa gtttaccttt tatggctaga gccgggtatg tgccgtcata taaaagagcg
     4861 cgcccagctt ttcagcctca ctttgagctc ctccacacgc agctagtgcg gaatatcatc
     4921 tgcttgtaac ccattctctt aagtcgacaa ccccccaaac ccaaggtgag ttggttttac
     4981 agtttttaag cgtttaatta tagtttctat ctattaatgt taagtaaaat gtgtaaatgg
     5041 ataaatatga tttgatgaag tctttagaca gttcataacg gtctctttgt agttatctac
     5101 taggtttgat ttataagtgg tggttctttt gataatctat gttacattta ataaatattg
     5161 gctgtatctt aacttttttg tagcgttagt ggatgtggca ggtgagaatg tcggtaattt
     5221 aacgtgacca gtcgtaggca cgacattgaa cattgaatag gccggtgtga aatgggtgtt
     5281 aagtcttaat ataaagttgt gttctggcta atctctcttt aacagccata aatgtgattt
     5341 ttaactacag ttttgagggt gctggtgagc atgttgcaca cttgatggat agccggcatg
     5401 ggaagttctt tgtgcaggca gtgctgcagc agggtgtgac ctactttagc tagccggcta
     5461 accagctctc atctgatgta acgtaaaccc catcaatcta acatcggctc attcgtgctt
     5521 tataagatcg aagatcttgt tattagtaac gggtattggc atttagtttt aacttggtta
     5581 attttctttt tgttatgtgt tctatttaaa tgtagcctgc agccccctct gtctttaatg
     5641 aagctgaaga ttaacgttaa cgttagtgaa gtgttgtatc cgctatgaaa gatttgaatt
     5701 ttagaggtgt ggtccacttt aataacggct gcggagttct ctggctgcac ttcagccgct
     5761 gtgactgtcg aagcagagcc catcagtttt tcaggatctc ggtgattttt gaggctagac
     5821 tatgaactga accgactgag agtgaccgtc cagaaatgct ttagtgtgaa gtctccgctc
     5881 acgaggagtc cgtggactga ctgcagatct gtgacgcagt aacacccggc ggcagacacc
     5941 cgttgaaatg cggttgtgta attgatcgca ggcgagggtg gaagaggatg tgaaacttca
     6001 tttgtgtaaa atttagggag tggtcctggt gtgatgaatg tcgaaatctg ttccttttta
     6061 ctgagcccta cgactctggc tgagtgccac accgccggca gccgcaaagc ggctcaaacc
     6121 attgcctttt atggtaataa tgagagaatg cagagggact tcctttgtct ggcatatctg
     6181 aggcgcgcgc tgtcactagc gcccaccagc ggtcagactg tagaatgcag agcaaaacag
     6241 gaagttgact ccagatggtc acatgcttgc tgaaacgcct ctccctggca gcagtgcaat
     6301 tctaacagtt tccctttctt ttacagttca gcctcaagtt tgtacaaaaa agcaggctgc
     6361 caccatgggc gtggccgacc tgatcaagaa gttcgagagc atcagcaagg aggagaaccc
     6421 agctttcttg tacaaagtgg ctgtgagcga gctgattaag gagaacatgc acatgaagct
     6481 gtacatggag ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa
     6541 gccctacgag ggcacccaga ccatgagaat caaggcggtc gagggcggcc ctctcccctt
     6601 cgccttcgac atcctggcta ccagcttcat gtacggcagc aaaaccttca tcaaccacac
     6661 ccagggcatc cccgacttct ttaagcagtc cttccccgag ggcttcacat gggagagagt
     6721 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
     6781 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat
     6841 gcagaagaaa acactcggct gggaggcctc caccgagacc ctgtaccccg ctgacggcgg
     6901 cctggaaggc agagccgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa
     6961 cttgaagacc acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta
     7021 ctatgtggac agaagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca
     7081 gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc acagatgagc
     7141 ggccgcgact ctagatcaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc
     7201 tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc
     7261 cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa
     7321 tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata
     7381 atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg
     7441 caattcaatt ggtttggtaa cctcatctac aacgtcaaga tcagaggggt gaacttccca
     7501 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagaccctg
     7561 taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct cgtgggcggg
     7621 ggccacctga tctgcaactt gaagaccaca tacagatcca agaaacccgc taagaacctc
     7681 aagatgcccg gcgtctacta tgtggacaga agactggaaa gaatcaagga ggccgacaaa
     7741 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
     7801 ctggggcaca gatgagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     7861 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     7921 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7981 catcacaaat ttcacaaata aagcattttt ttcactgcca actttattat acatagttga
     8041 taattcactg gccgtcgttt tacggtacca tcgatgatga tccagacatg ataagataca
     8101 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     8161 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     8221 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     8281 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctagatcag atctgcgaag
     8341 atacggccac gggtgctctt gatcctgtgg ctgattttgg actgtgctgc tcgcagctgc
     8401 tgatgaatca catacttcct ccattttctt ccactgattg actgttataa tttccctaat
     8461 ttccaggtca aggtgctgtg cattgtggta atagatgtga catgacgtca cttccaaagg
     8521 accaatgaac atgtctgacc aatttcatat aatgtgaaaa cgattttcat aggcagaata
     8581 aataacattt aaattaaact gggcatcagc gcaattcaat tggtttggta atagcaaggg
     8641 aaaatagaat gaagtgatct ccaaaaaata agtacttttt gactgtaaat aaaattgtaa
     8701 ggagtaaaaa gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat
     8761 ttttaattgt actcaagtaa agtaaaaatc cccaaaaata atacttaagt acagtaatca
     8821 agtaaaatta ctcaagtact ttacacctct ggttcttgac cccctacctt cagcaagccc
     8881 agcagatcca ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct
     8941 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9001 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9061 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9121 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9181 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9241 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9301 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9361 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9781 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10021 acgttaaggc attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10441 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10501 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10561 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10621 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10681 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10741 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10801 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10861 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10921 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10981 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11041 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11101 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
    11161 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cg
//
