LOCUS       sequence-401411-        9377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CLIP donor template for integrating GFP into IL2RG locus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Chavez M, Rane DA, Chen X, Qi LS
  TITLE     Stable expression of large transgenes via the knock-in of an 
            integrase-deficient lentivirus.
  JOURNAL   Nat Biomed Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x.
  PUBMED    37127707
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x."
COMMENT     Sequence Label: pSLQ8079 - IL2RG GFP-P2A CLIP Donor
FEATURES             Location/Qualifiers
     source          1..9377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     477..496
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     730..747
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(817..837)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        822..1151
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1002..1137
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2285..2350
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2480..2500
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2925..3059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2962..2981)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3016..3035
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3227..3860
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3907..4032
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4529..4762
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4947..4991
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5276..5393
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             5885..6547
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             5885..6547
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             6572..6628
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             7165..7398
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7423..7441)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7424..7441)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7731..7750)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7964..7983)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8083..8105
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8143..8161)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8229..8333
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8334..8402,8403..9194)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8552..8571)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9365..9377,1..576)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
       61 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
      121 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
      181 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      241 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
      301 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
      361 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      421 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      481 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      541 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      601 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      661 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      721 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      781 ctccccgcgc gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg
      841 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      901 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      961 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     1021 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     1081 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     1141 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
     1201 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
     1261 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
     1321 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
     1381 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
     1441 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg
     1501 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     1561 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     1621 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     1681 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     1741 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     1801 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
     1861 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     1921 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     1981 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
     2041 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
     2101 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
     2161 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa
     2221 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     2281 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     2341 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     2401 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     2461 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     2521 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     2581 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     2641 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     2701 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     2761 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     2821 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     2881 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     2941 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3001 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     3061 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     3121 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     3181 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     3241 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     3301 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     3361 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     3421 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     3481 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     3541 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     3601 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     3661 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     3721 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     3781 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     3841 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     3901 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     3961 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     4021 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     4081 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     4141 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     4201 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     4261 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     4321 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
     4381 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     4441 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     4501 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     4561 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     4621 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     4681 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     4741 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     4801 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     4861 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     4921 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     4981 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     5041 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     5101 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     5161 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     5221 ttcgattagt gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta
     5281 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     5341 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     5401 attacaggga cagcagagat ccagtttgga tcgataagct tgatatcgaa ttctggtaat
     5461 gatggcttca acatgggcat ggcatagaac ggtgatgtcg ggggtggggg ttcagaactt
     5521 ccattataga aggtaatgat ttagaggaga aggtggttga gaatggtgct agtggtagtg
     5581 aacagatcct tcccaggatc taggtgggct gaggattttt gagtctgtga cactattgta
     5641 tatccagctt tagtttctgt ttaccacctt acagcagcac ctaatctcct agaggactta
     5701 gcccgtgtca cacagcacat atttgccaca ccctctgtaa agccctggtt tataaggttc
     5761 tttccaccgg aagctatgac agaggaaacg tgtgggtggg gaggggtagt gggtgaggga
     5821 cccaggttcc tgacacagac agactacacc cagggaatga agagcaagcg ccatgtccgg
     5881 atcccccgcc atgaagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga
     5941 gctggtgggc ggcggagagg gcacccccga gcagggccgc atgaccaaca agatgaagag
     6001 caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt
     6061 ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa
     6121 cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag
     6181 cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg
     6241 cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga
     6301 gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct
     6361 gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat
     6421 ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct
     6481 gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc
     6541 cttcgccaga tctcgagtcc ccgggagcgg agctactaac ttcagcctgc tgaagcaggc
     6601 tggagacgtg gaggagaacc ctggacctat gttgaagcca tcattaccat tcacatccct
     6661 cttattcctg cagctgcccc tgctgggagt ggggctgaac acgacaattc tgacgcccaa
     6721 tgggaatgaa gacaccacag ctggtgggaa atctgggact ggagggggct ggtgagaagg
     6781 gtggctgtgg gaaggggccg tacagagatc tggtgcctgc cactggccat tacaatcatg
     6841 tgggcagaat tgaaaagtgg agtgggaagg gcaaggggga gggttccctg cctcacgcta
     6901 cttcttcttt ctttcttgtt tgtttgtttc tttctttctt ttgaggcagg gtctcactat
     6961 gttgcctagg ctggtctcaa actcctggcc tctagtgatc ctcctgcctc agcctttcaa
     7021 agcaccagga ttacagacat gagccaccat gttgaagcca tcattaccac ctcgagggaa
     7081 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7141 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7201 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7261 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7321 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7381 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
     7441 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
     7501 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
     7561 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
     7621 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
     7681 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     7741 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     7801 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     7861 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     7921 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     7981 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     8041 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     8101 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     8161 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     8221 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8281 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8341 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8401 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8461 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8521 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8581 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8641 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8701 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8761 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8821 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8881 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8941 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9001 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9061 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9121 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9181 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9241 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9301 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9361 cttcttgaga tcctttt
//
