LOCUS       sequence-401412-        9366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CLIP donor template for integrating GFP into ACTB locus. Encodes GFP
            with ACTB homology arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Chavez M, Rane DA, Chen X, Qi LS
  TITLE     Stable expression of large transgenes via the knock-in of an 
            integrase-deficient lentivirus.
  JOURNAL   Nat Biomed Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x.
  PUBMED    37127707
REFERENCE   2  (bases 1 to 9366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x."
COMMENT     Sequence Label: pSLQ9663 - ACTB GFP-P2A CLIP Donor
FEATURES             Location/Qualifiers
     source          1..9366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    230..355
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    852..1085
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1270..1314
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1599..1716
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             2252..2917
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             2252..2917
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             2942..2998
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             3477..3710
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3735..3753)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3736..3753)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4043..4062)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4276..4295)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4395..4417
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4455..4473)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4541..4645
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4646..4714,4715..5506)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4864..4883)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5677..6265
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6166..6185
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6419..6436
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6506..6526)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6511..6840
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6691..6826
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7974..8039
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8169..8189
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8614..8748
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8651..8670)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8705..8724
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(8916..9366,1..183)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
       61 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      121 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      181 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
      241 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
      301 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
      361 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
      421 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
      481 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
      541 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
      601 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
      661 gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga
      721 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
      781 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
      841 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
      901 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
      961 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1021 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1081 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     1141 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     1201 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     1261 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     1321 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     1381 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     1441 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     1501 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     1561 tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     1621 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     1681 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     1741 gatccagttt ggatcgataa gcttgatatc gaattccgcg gcgatatcat catccatggc
     1801 tgggactcaa ggcgctaact gcgcgtgcgt tctggggccc ggggtgccgc ggcctgggct
     1861 ggggcgaagg cgggctcggc cggaaggggt ggggtcgccg cggctcccgg gcgcttgcgc
     1921 gcacttcctg cccgagccgc tggccgcccg agggtgtggc cgctgcgtgc gcgcgcgccg
     1981 acccggcgct gtttgaaccg ggcggaggcg gggctggcgc ccggttggga gggggttggg
     2041 gcctggcttc ctgccgcgcg ccgcggggac gcctccgacc agtgtttgcc ttttatggta
     2101 ataacgcggc cggcccggct tcctttgtcc ccaatctggg cgcgcgccgg cgccccctgg
     2161 cggcctaagg actcggcgcg ccggaagtgg ccagggcggg ggcgacctcg gctcacagcg
     2221 cgcccggcta ttctcgcaac tgacaggatc catgcccgcc atgaagatcg agtgccgcat
     2281 caccggcacc ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccga
     2341 gcagggccgc atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta
     2401 cctgctgagc cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta
     2461 cgagaacccc ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa
     2521 gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt
     2581 gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga
     2641 caagatcatc cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct
     2701 ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt
     2761 ggacagccac atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc
     2821 catgttcgcc ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga
     2881 gtaccagcac gccttcaaga cccccatcgc cttcgccaga tctcgagtcc ccgggagcgg
     2941 agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctgc
     3001 cggctccggt accgatgatg atatcgcagc gctcgtcgtc gacaacggct ccggcatgtg
     3061 caaggccggc ttcgcgggcg acgatgcccc ccgggccgtc ttcccctcca tcgtggggcg
     3121 ccccaggcac caggtagggg agctggctgg gtggggcagc cccgggagcg ggcgggaggc
     3181 aagggcgctt tctctgcaca ggagcctccc ggtttccggg gtgggggctg cgcccgtgct
     3241 cagggcttct tgtcctttcc ttcccagggc gtgatggtgg gcatgggtca gaaggattcc
     3301 tatgtgggcg acgaggccca gagcaagaga ggcatcctca ccctgaagta ccccatcgcc
     3361 atggatgatg atatcgccgc gcctcgaggg aattaattcg agctcggtac ctttaagacc
     3421 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     3481 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     3541 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     3601 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     3661 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa
     3721 ttaattccgt gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg
     3781 tattaattgt agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc
     3841 ttactgaagc agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga
     3901 cgaaccttct gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc
     3961 agcagggtca tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc
     4021 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct
     4081 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     4141 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     4201 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga
     4261 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     4321 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4381 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4441 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4501 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4561 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4621 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4681 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4741 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4801 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4861 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4921 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4981 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5041 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5101 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5161 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5221 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5281 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5341 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5401 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5461 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5521 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5581 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5641 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5701 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5761 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5821 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5881 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5941 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6001 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6061 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6121 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6181 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6241 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6301 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6361 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6421 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6481 ttggccgatt cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6541 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6601 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6661 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6721 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     6781 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6841 aagcttggac acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc
     6901 agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc
     6961 agatctctat aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa
     7021 acaggctggg acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat
     7081 ccatgcacgt aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc
     7141 cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat
     7201 gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg
     7261 aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt
     7321 ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca
     7381 aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg
     7441 attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct
     7501 tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt
     7561 tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt
     7621 caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc
     7681 acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat
     7741 taaccgtctg atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc
     7801 acgtattgtg atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg
     7861 ctaccgtggc ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact
     7921 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
     7981 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
     8041 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
     8101 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
     8161 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
     8221 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
     8281 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct
     8341 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc
     8401 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt
     8461 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc
     8521 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     8581 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     8641 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8701 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     8761 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     8821 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     8881 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     8941 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     9001 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     9061 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     9121 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     9181 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     9241 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     9301 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     9361 cctgta
//
