LOCUS       sequence-401413-       10791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CLIP donor template to generate 2 knock-ins: GFP into ACTB, and 
            mCherry in RAB11A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Chavez M, Rane DA, Chen X, Qi LS
  TITLE     Stable expression of large transgenes via the knock-in of an 
            integrase-deficient lentivirus.
  JOURNAL   Nat Biomed Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x.
  PUBMED    37127707
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng. 2023 May 1. doi: 10.1038/s41551-023-01037-x."
COMMENT     Sequence Label: pSLQ8069 - ACTB GFP-P2A + RAB11A mCherry-P2A C...
FEATURES             Location/Qualifiers
     source          1..10791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    309..434
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    931..1164
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1349..1393
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1678..1795
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             2331..2996
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             2331..2996
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="MPAMKIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
     CDS             3021..3077
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3819..4523
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3961..3979)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4229..4248
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             4981..5214
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5239..5257)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5240..5257)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5547..5566)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5780..5799)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5899..5921
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5959..5977)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6045..6149
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6150..6218,6219..7010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6368..6387)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7181..7769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7670..7689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7923..7940
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8010..8030)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8015..8344
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8195..8330
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9478..9543
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9673..9693
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10118..10252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10155..10174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10209..10228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(10420..10791,1..262)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
       61 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
      121 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      181 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      241 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
      301 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
      361 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
      421 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
      481 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
      541 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
      601 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
      661 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
      721 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
      781 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
      841 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
      901 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
      961 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     1021 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     1081 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     1141 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     1201 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     1261 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     1321 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     1381 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     1441 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     1501 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     1561 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     1621 cattcgatta gtgaacggat ctcgacggta tcgccaaatg gcagtattca tccacaattt
     1681 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1741 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     1801 ttattacagg gacagcagag atccagtttg gatcgataag cttgatatcg aattccgcgg
     1861 cgatatcatc atccatggct gggactcaag gcgctaactg cgcgtgcgtt ctggggcccg
     1921 gggtgccgcg gcctgggctg gggcgaaggc gggctcggcc ggaaggggtg gggtcgccgc
     1981 ggctcccggg cgcttgcgcg cacttcctgc ccgagccgct ggccgcccga gggtgtggcc
     2041 gctgcgtgcg cgcgcgccga cccggcgctg tttgaaccgg gcggaggcgg ggctggcgcc
     2101 cggttgggag ggggttgggg cctggcttcc tgccgcgcgc cgcggggacg cctccgacca
     2161 gtgtttgcct tttatggtaa taacgcggcc ggcccggctt cctttgtccc caatctgggc
     2221 gcgcgccggc gccccctggc ggcctaagga ctcggcgcgc cggaagtggc cagggcgggg
     2281 gcgacctcgg ctcacagcgc gcccggctat tctcgcaact gacaggatcc atgcccgcca
     2341 tgaagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag ctggtgggcg
     2401 gcggagaggg cacccccgag cagggccgca tgaccaacaa gatgaagagc accaaaggcg
     2461 ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc taccacttcg
     2521 gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac ggcggctaca
     2581 ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc ttcagctacc
     2641 gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc ttccccgagg
     2701 acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag cacctgcacc
     2761 ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg cgcgacggcg
     2821 gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc caccccagca
     2881 tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg cacagcaaca
     2941 ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc ttcgccagat
     3001 ctcgagtccc cgggagcgga gctactaact tcagcctgct gaagcaggct ggagacgtgg
     3061 aggagaaccc tggacctgcc ggctccggta ccgatgatga tatcgcagcg ctcgtcgtcg
     3121 acaacggctc cggcatgtgc aaggccggct tcgcgggcga cgatgccccc cgggccgtct
     3181 tcccctccat cgtggggcgc cccaggcacc aggtagggga gctggctggg tggggcagcc
     3241 ccgggagcgg gcgggaggca agggcgcttt ctctgcacag gagcctcccg gtttccgggg
     3301 tgggggctgc gcccgtgctc agggcttctt gtcctttcct tcccagggcg tgatggtggg
     3361 catgggtcag aaggattcct atgtgggcga cgaggcccag agcaagagag gcatcctcac
     3421 cctgaagtac cccatcgcca tggatgatga tatcgccgcg cctcgagggg tagtcgtact
     3481 cgtcgtcgtg gggtagctag gagttccagg actcagtttc ccctttgagc ctcctttagc
     3541 gactaaagct tgaagcccca cgcatctcga ctctcgcgca caccgccctt gttgggctca
     3601 ggggcggggc gccgcccccg gaagtacttc cccttaaagg ctggggcctg ccggaaatgg
     3661 cgcagcggca gggaggggct cttcacccag tccggcagtt gaagctcggc gctcgggtta
     3721 cccctgcagc gacgccccct ggtcccacag ataccactgc tgctcccgcc ctttcgctcc
     3781 tcggccgcgc aatgggcgga tcgggtggga ctagtggcgt gagcaagggc gaggaggata
     3841 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     3901 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     3961 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     4021 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     4081 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     4141 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     4201 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     4261 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     4321 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     4381 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     4441 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     4501 gcggcatgga cgagctgtac aagggaaccg gtgctggaag tggtacacgc gacgacgagt
     4561 acgactacct ctttaaaggt gaggccatgg gctctcgcac tctacacagt cctcgttcgg
     4621 ggacccgggc cactcccggt ggaccctcgt gccggccacc cctgcactga tataggcctc
     4681 cctcagccct tcctttttgt gcggttccgt ctcctaccca gctcagcctc ttctcccccg
     4741 ctcagacagg ggtccccatc acatgccgct ctctgagcga cctctccata ggccttcgct
     4801 ggcctcagag cccctccctg cgtgtccttc ccctggcgga ctgccttctc ccacatcgtc
     4861 cacgacgacg agtacgacta cccatatgtc gagggattaa ttcgagctcg gtacctttaa
     4921 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4981 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     5041 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5101 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5161 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
     5221 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
     5281 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
     5341 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
     5401 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
     5461 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
     5521 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
     5581 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     5641 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     5701 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
     5761 tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5821 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5881 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5941 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6001 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6061 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6121 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6181 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6241 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6301 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6361 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6421 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6481 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6541 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6601 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6661 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6721 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6781 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6841 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6901 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6961 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7021 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7081 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7141 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7201 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7261 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7321 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7381 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7441 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7501 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7561 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7621 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7681 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7741 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7801 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7861 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7921 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7981 cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8041 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8101 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8161 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8221 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8281 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8341 caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg
     8401 cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg
     8461 cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag
     8521 ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc
     8581 agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta
     8641 ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg
     8701 tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt
     8761 gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac
     8821 cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt
     8881 cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac
     8941 ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta
     9001 atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat
     9061 agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc
     9121 tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg
     9181 gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca
     9241 tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc
     9301 aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga
     9361 ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct
     9421 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
     9481 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
     9541 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
     9601 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
     9661 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
     9721 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
     9781 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta
     9841 acctttataa gtaggcataa cagttataat cataacatac tgttttttct tactccacac
     9901 aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta
     9961 atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat
    10021 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
    10081 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
    10141 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10201 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    10261 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    10321 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    10381 aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc
    10441 aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
    10501 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
    10561 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
    10621 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
    10681 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
    10741 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt c
//
