LOCUS       sequence-401416-       19181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CPL2-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19181)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Li Z, Feng S, Kuo HY, Kan RL, 
            Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, 
            Wohlschlegel J, Jacobsen SE
  TITLE     A gene silencing screen uncovers diverse tools for targeted gene 
            repression in Arabidopsis.
  JOURNAL   Nat Plants. 2023 Mar 6. doi: 10.1038/s41477-023-01362-8.
  PUBMED    36879017
REFERENCE   2  (bases 1 to 19181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2023 Mar 6. doi: 10.1038/s41477-023-01362-8."
COMMENT     Sequence Label: pEG302-CPL2-ZF
FEATURES             Location/Qualifiers
     source          1..19181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5163..5185
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5177..5194
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5178..5194
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5217..5391
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5394..6359)
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(5397..6359)
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     terminator      complement(7820..8527)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(8607..8651)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(9177..9200)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(9207..9278)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    9337..9361
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(13078..13089)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(15153..15177)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        15229..15609
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             15615..16166
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      16176..16428
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    16514..16538
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             16963..17757
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     5041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     5161 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcagggatc
     5221 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
     5281 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
     5341 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa agcttatttt
     5401 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc atcctgctca
     5461 agtaataaag ccccctgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     5521 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgacagataa
     5581 ggtgccaatc cttcttgttc gaagagttcc aacgcagcac gtaattcacg tattagcatg
     5641 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     5701 ttaacgacac tctcttcaac acggcgcatt gccatgttga tcccggctcc aatgactatt
     5761 tgcgccgcat cgccagtttt gccagtcagc tccaccagaa tgcctgccag cttgcgatcc
     5821 tgcagataga ggtcattagg ccatttaaca cgaactttat ctgcacccag cttgcgtaat
     5881 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     5941 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccga
     6001 ccccggcgac cacggccagc ctgctggtat tctgcaatgc aagcatcgcc cgatttaagc
     6061 tctccgatac gatcaagaag gtactgattc gtggagtcaa tcactggcag cacggctaca
     6121 ctaccgccat ccagctgacc caatatctgt ttagcattaa gtaactggat aggctcaggc
     6181 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     6241 atgtgtttat taatagccgc ccggctcatt cccagcgttt cacccaactg ctcgccagag
     6301 tgaaattcac cgttcgctaa cagggcaatc aatttcagtg gcacggtgtt atccttcatc
     6361 tcgaggtcac acacgattgg ttgaatatca tcagcctcag ccatttttta tgagctgcaa
     6421 acacacaaaa agagttcaat acagtcaaat aaaccctgat tgaatcagaa atagctttct
     6481 gttcaacgta cgacactaca aatcctaaag tttcgaagaa tctatacaca aacttcatct
     6541 aaccttaaac agtgttcaga ttcaatctaa cattgacagt gttcagattc aatctaactt
     6601 caacagttca gatttcaatt cacatataca aatcatttcc caggtaacat gaacatggat
     6661 ctctccatca aggtcaagcc aaattctggt agctataatc gagctaactg atcggtcaac
     6721 ttagatcgat tctagtaaaa ccaaaagatt tagtggaggt tcacagatcc aaattaaacg
     6781 atatccagat ctcaaggaaa ttgagaaaac aagatcgaga tccagcaaat aaagtagatc
     6841 cagaaagttc ctattacctt ggaaagaaag agcggaagaa gatgagattg aggaagattc
     6901 aaaacggaga atgaatctcc tggattatct cttactttct ctcttagtct tcttccttgt
     6961 tcttctctgt caagtcgccg gagattcaaa acggctgatg aaagtgagga ggacaacgag
     7021 acaattcaaa gcggagagga aaatatatga atttatatag gcgggtttat ctcttacaac
     7081 tttattttcg gcctttcaaa aaaataatta aaatcgacag acacgaatca tttcgaccac
     7141 aggtaaagat aacgtgacct ggctgtcaga cagccttttc cctcgtgtta actaattttt
     7201 aaactaatta atcatctcag cccttggatt agttcttttg ctttgatggc ttcatgactg
     7261 tgacctgctc gatccgcgtg ttacatgaca gctccgtttt tttagtggtt aacttaaacc
     7321 gagtcaatcc aggcaacgtt agtcgtcgtc gtggttggct tgttcaatta gatttcatac
     7381 aattcaacgt aatttaattc gttttctatt agaattgtat cataattaat tcagaccgtg
     7441 aaagaaagtg tctttcatga tgtgtttatg gatatttata caataagata caatgtttca
     7501 tcatattcac tattcacgat tagtatgtac attaaataat ggctactact acatccgaac
     7561 tcgtcaaaac gattctgaat caattataca tatgctgact cttgcataca taaaaaatag
     7621 ttgtttaaat tttgtctaac taatgtttgg tataagtata atgttgagtt gagataccaa
     7681 ttacatcgag tctagccatt ttgtcgtgcc atattcgtca aaactttctt acataatgat
     7741 aacctagatc tagatgagat atgtatcaat gtatttgaga tcataattaa gttcgttcta
     7801 aattttgtcc ctgcaggtcc tgctgagcct cgacatgttg tcgcaaaatt cgccctggac
     7861 ccgcccaacg atttgtcgtc actgtcaagg tttgacctgc acttcatttg gggcccacat
     7921 acaccaaaaa aatgctgcat aattctcggg gcagcaagtc ggttacccgg ccgccgtgct
     7981 ggaccgggtt gaatggtgcc cgtaactttc ggtagagcgg acggccaata ctcaacttca
     8041 aggaatctca cccatgcgcg ccggcgggga accggagttc ccttcagtga gcgttattag
     8101 ttcgccgctc ggtgtgtcgt agatactagc ccctggggca cttttgaaat ttgaataaga
     8161 tttatgtaat cagtctttta ggtttgaccg gttctgccgc tttttttaaa attggatttg
     8221 taataataaa acgcaattgt ttgttattgt ggcgctctat catagatgtc gctataaacc
     8281 tattcagcac aatatattgt tttcatttta atattgtaca tataagtagt agggtacaat
     8341 cagtaaattg aacggagaat attattcata aaaatacgat agtaacgggt gatatattca
     8401 ttagaatgaa ccgaaaccgg cggtaaggat ctgagctaca catgctcagg ttttttacaa
     8461 cgtgcacaac agaattgaaa gcaaatatca tgcgatcata ggcgtctcgc atatctcatt
     8521 aaagcaggac tctagggact agtcccgggt cttaattaac tctctagagg cgcgccaagc
     8581 ttctactact aagatccacc agtatcctca tgccattcaa tcttctgagc ttcgaagata
     8641 tcgttaagtc caccagccat agatccctcg agtccggtgt gagtcctttg atgctcggtg
     8701 agcttatcac tcctagagaa agattttcca cactcagggc atttataagg tttttctcca
     8761 gtgtgtgtcc tttgatgagc cctaaggttt gctctctgag agaatgattt tccacactca
     8821 gggcatttat aaggtttttc accagtatgg gtcctttggt gcctaacaag gtttccagat
     8881 gttgagaatg attttccaca ctcagggcat ttataaggtt tttctccggt atgagtcctc
     8941 tgatgcctaa caagagatga agattgtgag aatgattttc cacactcagg gcatttataa
     9001 ggtttttcac cagtatgggt cctctgatgg gtggtaagat gatcactcct actgaatgat
     9061 tttccacact cagggcattt ataaggtttt tcaccggtat gggtcctttg gtgcctccta
     9121 agatctccag attgagagaa agattttcca cactcagggc atttataagg ctcgagaggt
     9181 ccttggaaaa gaacctcaag ggatcctttg tcgtcatcgt ctttgtagtc tttgtcgtca
     9241 tcgtctttgt agtctttgtc gtcatcgtct ttgtagtcgg taccaagctt ggcgcgcccg
     9301 tcccgggtct taattaactc tctagactca cctagcacca ctttgtacaa gaaagctggg
     9361 tcggcgcgcc cacccttcaa ctctgaaatg ttttctttag gcgcttcctc ttcaagtcct
     9421 ttgtttgata catcttcact gctttcccac aaaaatccat tatcattttc cggacctttc
     9481 tctgtttctc tagctagagg tgccacatgt gcaacatact tttctgcaca aagcaacaca
     9541 aagaagattt accaatctgt aaccaaattt gctcactaca caaaagacta agaacaatct
     9601 gcagacttaa tcaagtggtg aaatagatcc acagttatta attcccctag aagaaacttt
     9661 accagccaaa cttcgaagag cattctcagc cgcttgttga tgtgcatctt tcttagtttt
     9721 tgccatccca atgcctatct tctcacccgt gaacaacacc tttgtaaaat taacaacaac
     9781 gattaaaaac aaatataagt aaagctaaca atagacaaag atgcttcaat aaactgatcc
     9841 agctgacctc aactgaaaac tgtaactctt tgttggtact tatcacagtc ctaaactcca
     9901 cctgcaaaat cgtacatcaa ttaatcagaa tccattaaaa gagaagaagc aatggcttgc
     9961 gtggttagtg aatatgcacc tttgaaccac atcttcttcc aatttcttgc agtgcactca
    10021 caaataaatg agattgcccg ccattactct ttccaccatc tgtatgatgt tctctaccat
    10081 ttgacaccaa atgattctgg gatattccac cttctgcaaa tagtttaaac aagtgccaat
    10141 gttaaaaagg tattaatgca ccttattatt caagtagctg ttacagattc agtagaaaga
    10201 caatacatca gatagggaaa ccaacaattc tctaatccta agtcaatatc taatgtagct
    10261 ttagagcggt acactgaaaa caagcttcac ggaggaaaca agaaattcag ggatagacta
    10321 atcatcttta atatcagctt gattgcatta gtgttgaaac tagaacatac ctgttgtttg
    10381 acgtgaagga ttttgccttt tcaggtcatc atccattgct acctgcaaga aaactatcag
    10441 accgtagaca acatactttg atgtcaaaag agagacactt agaacgcaaa actaacctct
    10501 tcactcctca agtgggatgg atgtgcaaat ggaccaacag gagcagaacc tggtgttcca
    10561 tgaggaaatt gacttggata aagtcctgaa ggccgaccag gaaaagaagg tctattctca
    10621 tcatctacca accaaccacc cggagaatgc atagacgagg aaggtggctg cataggaatt
    10681 ttagccagaa tggggggttg attaaagttt tggttcctta tatctacacc aggcctcatc
    10741 ataaggggtc ttccaccatc agaaaaagtg aagccatccc gcctaggagc tccaagcaaa
    10801 ctaggttcta gattgtgatt gaagacaaga gatagagaac gtcagagaca aacatatacg
    10861 tgcaaaataa ctgtacagtt caaaatttaa agttatatta agtcaaaagt ttgtactatg
    10921 agaaggtaac agtgctgctg cagttgccgt gcttgcatta tttggtataa ctgctatctg
    10981 cggcttaggg gtttcaggtt tctgctcagc gttacttgtt gcaggaagag tagaatgatc
    11041 agctgcagcc ttctcatccc agaaattgaa aagtcaacag aaatcaaatt acaactgaca
    11101 caaaagctat gcaccaaaac atattttagt taattaatcc caaattttgt atatatttta
    11161 ctaaccgctt ggtttagtct tcgttccact tcacccccac acattccttc gttaattgga
    11221 ggagcattta tgtttccatt tgaagcaaag ccaggatcct acagacagat atagctagat
    11281 tacatattgt agttggggca aattagcaat atgttaacaa tgataaagtt ctaactacag
    11341 ttacctctat aacgacgtag ttgctcacat ccggtgatgg aggtaaattt tccacatcat
    11401 cttcatagta aaccaaagat atactactca ttaaactctc atcaaattcc ctgccataaa
    11461 agtctagaaa cagtaagaac aaccagcatg aattcattac ataaaaataa aaaaaaaaca
    11521 taattttttt ttccaactta cttgaaaaag taacctctga cattgcatgc aacatttctt
    11581 gctacgcaga gatgtggaac gacaagggct gtctgataaa tgggcatacg aaaatggtta
    11641 gatacccact tggaaaatta agtgatacag aatttaattc acaactaaat gcaagagcaa
    11701 ccacatgcct ctgcttgtgg agcataataa ggaagatatg cagaaacaac atgaacccgt
    11761 ggttgatcct tgtcctccca aaccttcata cggtcatcaa tgaccattgc cattttcggg
    11821 tggcaaatgc caccattgaa gacacttaac aacgatttct tggcgtctga tatgcaaaga
    11881 gcaagacata aaaaaattgt tagttcgagt ttcagagaga gacagagaca gatagttggg
    11941 taaaaaatga tcaggttcat ctcttaatac taaaaactga gaggtcggca aatcaatcag
    12001 gcacgataga gggagggaga agaggcacat tatattaaac aattcaccac aaaaagagta
    12061 atgaaagcta cactaatgga gcatggtggc tatgtaccaa aaccaagatc aagggtatca
    12121 aagaagccag agttggtttt gacctggttt tacgcagacg atacgatctc gaagctcctt
    12181 gagacttatc aagtgtgctt ccggatcaag cagtctccac atttccagag cataatccct
    12241 ttcagccatg gtacaaacgt aaacttcaaa acgtttccta gttttggctg tcaaataact
    12301 tcttaactcc tcccatgctg gtctcagttt tacaagcaca ctagtgtcac ggatctgcat
    12361 cagcaataac ttggtttcag ttccaacaat attaaagcta aatcactcaa atatacttaa
    12421 gcgaccttat cctatcacat gccagagaag gtaaatccaa agcatcaaat aagatgaaat
    12481 gaataaatat ccagccacaa cagagatgaa tcttctaacc tctggcttaa tccgagtaag
    12541 cacagtattc ttctccggca atctaatcac gggtctacaa actttctctt gtccatcaga
    12601 agttgggcgg acttcctcag gttgagcctt taacaaaact ccgttatcaa aagcatagtc
    12661 attatcaata tactgcttca gcagcatcct atcatccata taccttttaa gttctgctga
    12721 catcccatta atcctcacag gatccatctc acgtgaaatc caagatttaa gagcctcgat
    12781 tctatcctca aatgacttca tagtatttgc aacaatcaaa gtctcatcca aatcaaacac
    12841 aatagacaaa caccgagtat taagcatcct taagcacgaa tcataaagac ctgaaggaac
    12901 cgaaaaacac caaaaacacg ggaacttctt ctccttactc ggcatcgcca ctaaatgaat
    12961 ctcctcatct ccgagcataa ccactgcagt ctgtattcac aaaatcaaac tagtgaatca
    13021 ctcctaacaa ttggaaaaca tccaaaatca gctaaatgtc atttcggaaa tctcaaacct
    13081 accttcaatt catggaaaca gactgcgtgt agatgcatta actcttgaga tttcaccgga
    13141 gccgatgact caagcttgca tcgaacggca aaagaagcaa tcgtctgaag aatcgcgagc
    13201 ggaggacatc tctcaccagc cggagataga tggtggattc gaatctcatc gttcgggaat
    13261 cgaaattcat gacttgaaga tacatggttc acatcaagtt cccccaaacg taaatctccg
    13321 tgataaacca cggatttatg acccaaacga ttcatcgtga attcgttcaa ttcacacttc
    13381 tgaaggacga atcaggtagt gaagataaac agtttctggg aaaatgcgtg agagaatctg
    13441 cagattcaat ttctctcaaa cttcgctact tttctctgtg atttttctcc gcaaatgagg
    13501 gtttttctgg gtctccttaa acatgagaac caaagtaggc tcagatatat atttataggc
    13561 gagagaagcg ttttattgtt acgacatcga ttttaatgta aattacgaga aaacctgaga
    13621 cttctttaca cgtggcgtca gatgcattat cctgttttca ccaacatcgc ttgtcagccg
    13681 tcaattaccg ttcctaacaa aataaaatat tcgagaatta ttttttgtgt gtatgacgaa
    13741 atcggattag gtcgataatt ttggttgact tcgaacaaat ggatagttaa tgtttacaac
    13801 tatatattgt aaaatgtgga aacgtattca ataacttact ttagaagtaa ataaaaattg
    13861 tgaaaaaaaa acggaagagg atatatatat ggtaaaatca caaaaatcaa ataagaacat
    13921 aaagttggtt ggcaaaaaaa gagaagacat caagttgcga gtcttgtgac gtttgagtaa
    13981 gaaagggaca aatcaatttt atacaatctg tatcatcata ttattatggg tgtgttggca
    14041 aattagtcac ttttgaaaac ataattatgg agttgggcaa gttactattt ataattggca
    14101 aaacaggtaa ctcactttgg gcgtgtaata gacgagcgtg aaatagaggt ttggggtggg
    14161 gtaagtgatg tgttttgaaa ttaccgaact atccttttga caaagaagaa agataaaagt
    14221 gaaatattct tttttttttt tatttctttt taataacccc tccaatttaa taattgaaat
    14281 acttatcaat ataaatttat tttctaaact aattcatata cataaaaatc tatgtatttt
    14341 tctctataat ttgatatgca ttttgttaat attttttaat atattacaga cattttatca
    14401 gtagtcggta cacttaatta tgtgtacaga ctttattttg tatgttgcat tagctgtaca
    14461 gagtatataa ttgtatggag tatacaatta tcagaagtca cttttcactg ttgattcgta
    14521 cacttagacc tgtacaatat ttttagtgta tatatatata tataaatata cacaacatac
    14581 acttacatct gtacactaaa aagtgttgta cagatctaag tgtacgaatc aaacagttaa
    14641 aagtgactac tttgcaaatt gtgaatagta aaatgactat tttcccaatt atggctggac
    14701 gatttcaatt tgtacttatt aaataagttg tataaataaa aatctgtaca cataattaac
    14761 tgtactgact actaataaaa atgtttatat aatatattta aaattattaa caaaatgcat
    14821 atcaaattat agagaaaaaa atatagattt ttatgtatct gaattaattt agaaaataaa
    14881 tttacattga taagcatttc aattattaga ttgaagaggt tattaaaaaa taaataaata
    14941 tatatatata taaagaaata caaagaatga gagtatgagg agagagaaat gttgaatcta
    15001 aaagcacaaa tcccaattgc tttcaacgac attagagaag aagaagatta gtggcatatt
    15061 tgtaaataat agagttggta tggggtatat ttgattttgt ctgtaatagg gtcaaaggtc
    15121 agtgactaaa aaggtgaagg gggcggccgc ggagcctgct tttttgtaca aacttgttga
    15181 tgatcccggg cccgcggtac cgtcgactgc agaattcaat tctgagattt ttcaaatcag
    15241 tgcgcaagac gtgacgtaag tatccgagtc agtttttatt tttctactaa tttggtcgtt
    15301 tatttcggcg tgtaggacat ggcaaccggg cctgaatttc gcgggtattc tgtttctatt
    15361 ccaacttttt cttgatccgc agccattaac gacttttgaa tagatacgct gacacgccaa
    15421 gcctcgctag tcaaaagtgt accaaacaac gctttacagc aagaacggaa tgcgcgtgac
    15481 gctcgcggtg acgccatttc gccttttcag aaatggataa atagccttgc ttcctattat
    15541 atcttcccaa attaccaata cattacacta gcatctgaat ttcataacca atctcgatac
    15601 accaaatcga atcgatgagc ccagaacgac gcccggccga catccgccgt gccaccgagg
    15661 cggacatgcc ggcggtctgc accatcgtca accactacat cgagacaagc acggtcaact
    15721 tccgtaccga gccgcaggaa ccgcaggagt ggacggacga cctcgtccgt ctgcgggagc
    15781 gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc cggcatcgcc tacgcgggcc
    15841 cctggaaggc acgcaacgcc tacgactgga cggccgagtc gaccgtgtac gtctcccccc
    15901 gccaccagcg gacgggactg ggctccacgc tctacaccca cctgctgaag tccctggagg
    15961 cacagggctt caagagcgtg gtcgctgtca tcgggctgcc caacgacccg agcgtgcgca
    16021 tgcacgaggc gctcggatat gccccccgcg gcatgctgcg ggcggccggc ttcaagcacg
    16081 ggaactggca tgacgtgggt ttctggcagc tggacttcag cctgccggta ccgccccgtc
    16141 cggtcctgcc cgtcaccgag atttgactgc agaatcttgg actcccatgt tggcaaaggc
    16201 aaccaaacaa acaatgaatg atccgctcct gcatatgggg cggtttgagt atttcaactg
    16261 ccatttgggc tgaattgaag acatgctcct gtcagaaatt ccgtgatctt actcaatatt
    16321 cagtaatctc ggccaatatc ctaaatgtgc gtggctttat ctgtctttgt attgtttcat
    16381 caattcatgt aacgtttgct tttcttatga attttcaaat aaattatcag atccgagctc
    16441 gaattaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc
    16501 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg
    16561 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg
    16621 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa
    16681 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg
    16741 attgtaacga tgacagagcg ttgctgcctg tgatcaccgc ggtttcaaaa tcggctccgt
    16801 cgatactatg ttatacgcca actttgaaaa caactttgaa aaagctgttt tctggtattt
    16861 aaggttttag aatgcaagga acagtgaatt ggagttcgtc ttgttataat tagcttcttg
    16921 gggtatcttt aaatactgta gaaaagagga aggaaataat aaatggctaa aatgagaata
    16981 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
    17041 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
    17101 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
    17161 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
    17221 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
    17281 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
    17341 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
    17401 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
    17461 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
    17521 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
    17581 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
    17641 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
    17701 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
    17761 ctagaatgca tgaccaaaat cccttaacgc tgagagatcc cctcataatt tccccaaagc
    17821 gtaaccatgt gtgaataaat tttgagctag tagggttgca gccacgagta agtcttccct
    17881 tgttattgtg tagccagaat gccgcaaaac ttccatgcct aagcgaactg ttgagagtac
    17941 gtttcgattt ctgactgtgt tagcctggaa gtgcttgtcc caaccttgtt tctgagcatg
    18001 aacgcccgca agccaacatg ttagttgaag catcagggcg attagcagca tgatatcaaa
    18061 acgctctgag ctgctcgttc ggctatggcg taggcctagt ccgtaggcag gacttttcaa
    18121 gtctcggaag gtttcttcaa tctgcattcg cttcgaatag atattaacaa gttgtttggg
    18181 tgttcgaatt tcaacaggta agttagttgc tagaatccat ggctcctttg ccgacgctga
    18241 gtagatttta ggtgacgggt ggtgacaatg agtccgtgtc gagcgctgat tttttcggcc
    18301 tttagagcga gatttataca atagaatttg gcatgagatt ggattgcttt tagtcagcct
    18361 cttatagcct aaagtctttg agtgactaga tgacatatca tgtaagttgc tgataggttt
    18421 ccagttttcc gctcctaggt ctgcatattg tacttttcct cttactcgac ttaaccagta
    18481 ccaacccagc ttctcaacgg atttatacca tggcacttta aagccagcat cactgacaat
    18541 gagcggtgtg gtgttactcg gtagaatgct cgcaaggtcg gctagaaatt ggtcatgagc
    18601 tttctttgaa cattgctctg aaagcgggaa cgctttctca taaagagtaa cagaacgacc
    18661 gtgtagtgcg actgaagctc gcaataccat aagccgtttt tgctcacgga tatcagacca
    18721 gtcaacaagt acaatgggca tcgtattgcc cgaacagata aagctagcat gccaacggta
    18781 tacagcgagt cgctctttgt ggaggtgacg attacctaac aatcggtcga ttcgtttgat
    18841 gttatgtttt gttctcgctt tggttggcag gttacggcca agttcggtaa gagtgagagt
    18901 tttacagtca agtaaggcgt ggcaagccaa cgttaagctg ttgagtcgtt ttaagtgtaa
    18961 ttcggggcag aattggtaaa gagagtcgtg taaaatatcg agttcgcaca ttttgttgtc
    19021 tgattattga tttttggcga aaccatttga tcatatgaca agatgtgtat ctaccttaac
    19081 ttaatgattt tgataaaaat cattagggga ttcatcagcc cttaacgtga gttttcgttc
    19141 cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
