LOCUS       sequence-401418-       17464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ELF7-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17464)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Li Z, Feng S, Kuo HY, Kan RL, 
            Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, 
            Wohlschlegel J, Jacobsen SE
  TITLE     A gene silencing screen uncovers diverse tools for targeted gene 
            repression in Arabidopsis.
  JOURNAL   Nat Plants. 2023 Mar 6. doi: 10.1038/s41477-023-01362-8.
  PUBMED    36879017
REFERENCE   2  (bases 1 to 17464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2023 Mar 6. doi: 10.1038/s41477-023-01362-8."
COMMENT     Sequence Label: pEG302-ELF7-ZF
FEATURES             Location/Qualifiers
     source          1..17464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5163..5185
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5177..5194
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5178..5194
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5217..5391
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5394..6359)
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(5397..6359)
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     terminator      complement(7820..8527)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(8607..8651)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(9177..9200)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(9207..9278)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    9337..9361
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(13436..13460)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        13512..13892
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             13898..14449
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      14459..14711
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    14797..14821
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             15246..16040
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     5041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     5161 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcagggatc
     5221 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
     5281 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
     5341 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa agcttatttt
     5401 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc atcctgctca
     5461 agtaataaag ccccctgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     5521 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgacagataa
     5581 ggtgccaatc cttcttgttc gaagagttcc aacgcagcac gtaattcacg tattagcatg
     5641 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     5701 ttaacgacac tctcttcaac acggcgcatt gccatgttga tcccggctcc aatgactatt
     5761 tgcgccgcat cgccagtttt gccagtcagc tccaccagaa tgcctgccag cttgcgatcc
     5821 tgcagataga ggtcattagg ccatttaaca cgaactttat ctgcacccag cttgcgtaat
     5881 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     5941 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccga
     6001 ccccggcgac cacggccagc ctgctggtat tctgcaatgc aagcatcgcc cgatttaagc
     6061 tctccgatac gatcaagaag gtactgattc gtggagtcaa tcactggcag cacggctaca
     6121 ctaccgccat ccagctgacc caatatctgt ttagcattaa gtaactggat aggctcaggc
     6181 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     6241 atgtgtttat taatagccgc ccggctcatt cccagcgttt cacccaactg ctcgccagag
     6301 tgaaattcac cgttcgctaa cagggcaatc aatttcagtg gcacggtgtt atccttcatc
     6361 tcgaggtcac acacgattgg ttgaatatca tcagcctcag ccatttttta tgagctgcaa
     6421 acacacaaaa agagttcaat acagtcaaat aaaccctgat tgaatcagaa atagctttct
     6481 gttcaacgta cgacactaca aatcctaaag tttcgaagaa tctatacaca aacttcatct
     6541 aaccttaaac agtgttcaga ttcaatctaa cattgacagt gttcagattc aatctaactt
     6601 caacagttca gatttcaatt cacatataca aatcatttcc caggtaacat gaacatggat
     6661 ctctccatca aggtcaagcc aaattctggt agctataatc gagctaactg atcggtcaac
     6721 ttagatcgat tctagtaaaa ccaaaagatt tagtggaggt tcacagatcc aaattaaacg
     6781 atatccagat ctcaaggaaa ttgagaaaac aagatcgaga tccagcaaat aaagtagatc
     6841 cagaaagttc ctattacctt ggaaagaaag agcggaagaa gatgagattg aggaagattc
     6901 aaaacggaga atgaatctcc tggattatct cttactttct ctcttagtct tcttccttgt
     6961 tcttctctgt caagtcgccg gagattcaaa acggctgatg aaagtgagga ggacaacgag
     7021 acaattcaaa gcggagagga aaatatatga atttatatag gcgggtttat ctcttacaac
     7081 tttattttcg gcctttcaaa aaaataatta aaatcgacag acacgaatca tttcgaccac
     7141 aggtaaagat aacgtgacct ggctgtcaga cagccttttc cctcgtgtta actaattttt
     7201 aaactaatta atcatctcag cccttggatt agttcttttg ctttgatggc ttcatgactg
     7261 tgacctgctc gatccgcgtg ttacatgaca gctccgtttt tttagtggtt aacttaaacc
     7321 gagtcaatcc aggcaacgtt agtcgtcgtc gtggttggct tgttcaatta gatttcatac
     7381 aattcaacgt aatttaattc gttttctatt agaattgtat cataattaat tcagaccgtg
     7441 aaagaaagtg tctttcatga tgtgtttatg gatatttata caataagata caatgtttca
     7501 tcatattcac tattcacgat tagtatgtac attaaataat ggctactact acatccgaac
     7561 tcgtcaaaac gattctgaat caattataca tatgctgact cttgcataca taaaaaatag
     7621 ttgtttaaat tttgtctaac taatgtttgg tataagtata atgttgagtt gagataccaa
     7681 ttacatcgag tctagccatt ttgtcgtgcc atattcgtca aaactttctt acataatgat
     7741 aacctagatc tagatgagat atgtatcaat gtatttgaga tcataattaa gttcgttcta
     7801 aattttgtcc ctgcaggtcc tgctgagcct cgacatgttg tcgcaaaatt cgccctggac
     7861 ccgcccaacg atttgtcgtc actgtcaagg tttgacctgc acttcatttg gggcccacat
     7921 acaccaaaaa aatgctgcat aattctcggg gcagcaagtc ggttacccgg ccgccgtgct
     7981 ggaccgggtt gaatggtgcc cgtaactttc ggtagagcgg acggccaata ctcaacttca
     8041 aggaatctca cccatgcgcg ccggcgggga accggagttc ccttcagtga gcgttattag
     8101 ttcgccgctc ggtgtgtcgt agatactagc ccctggggca cttttgaaat ttgaataaga
     8161 tttatgtaat cagtctttta ggtttgaccg gttctgccgc tttttttaaa attggatttg
     8221 taataataaa acgcaattgt ttgttattgt ggcgctctat catagatgtc gctataaacc
     8281 tattcagcac aatatattgt tttcatttta atattgtaca tataagtagt agggtacaat
     8341 cagtaaattg aacggagaat attattcata aaaatacgat agtaacgggt gatatattca
     8401 ttagaatgaa ccgaaaccgg cggtaaggat ctgagctaca catgctcagg ttttttacaa
     8461 cgtgcacaac agaattgaaa gcaaatatca tgcgatcata ggcgtctcgc atatctcatt
     8521 aaagcaggac tctagggact agtcccgggt cttaattaac tctctagagg cgcgccaagc
     8581 ttctactact aagatccacc agtatcctca tgccattcaa tcttctgagc ttcgaagata
     8641 tcgttaagtc caccagccat agatccctcg agtccggtgt gagtcctttg atgctcggtg
     8701 agcttatcac tcctagagaa agattttcca cactcagggc atttataagg tttttctcca
     8761 gtgtgtgtcc tttgatgagc cctaaggttt gctctctgag agaatgattt tccacactca
     8821 gggcatttat aaggtttttc accagtatgg gtcctttggt gcctaacaag gtttccagat
     8881 gttgagaatg attttccaca ctcagggcat ttataaggtt tttctccggt atgagtcctc
     8941 tgatgcctaa caagagatga agattgtgag aatgattttc cacactcagg gcatttataa
     9001 ggtttttcac cagtatgggt cctctgatgg gtggtaagat gatcactcct actgaatgat
     9061 tttccacact cagggcattt ataaggtttt tcaccggtat gggtcctttg gtgcctccta
     9121 agatctccag attgagagaa agattttcca cactcagggc atttataagg ctcgagaggt
     9181 ccttggaaaa gaacctcaag ggatcctttg tcgtcatcgt ctttgtagtc tttgtcgtca
     9241 tcgtctttgt agtctttgtc gtcatcgtct ttgtagtcgg taccaagctt ggcgcgcccg
     9301 tcccgggtct taattaactc tctagactca cctagcacca ctttgtacaa gaaagctggg
     9361 tcggcgcgcc cacccttttc agaataatca tcctcattac catcactata ttgattggca
     9421 tcttgttccg gctcgtgttt ccaagatcta ccaaggccgc cttcgtcctc taatcttctc
     9481 atcttggagc tcgaagcacc aaccctagaa gagtaaacct acatccaaag ccccaaagtc
     9541 caaaggaatt ggtaagagaa ctagaacttt ccacgtgtta ctcttttaaa ggctaaatgt
     9601 atggatttac tatacccctg aatctttatg ttctatcact gatacagttg atctccgcct
     9661 tacagttacc cttgaaggta ctggaaagtg ttcaatctca tcagaagatc ttccttccct
     9721 ggcccttttc ttcctcaagt tcaacctcat gggtaggggc tgccaaattc tcaacggtca
     9781 tcccaaagta ttatagacag aagaaaaaaa agatacacaa ctggaatatg taacaaaaac
     9841 cgatgcaata ccagatagct agcggtccca ttgtcaaatg ataccaggta tgtgccagga
     9901 tcgtttgcat tgggttgtac ctgttataac atcaggtgac atatttattt caatctgcaa
     9961 ctcaaacaat caaaatttca cttaaaccac attggctata gtacatacat cccagaggta
    10021 ttcgcgaacc caagtgtaag agatttcttc gttttcatca tggatatcct tagacaactg
    10081 gcacagcaac ataaattgat aacacatcaa tcaaactgaa cttttcttga cttgtcaaaa
    10141 tttgtgtaaa agagagatca gtttatggag aggcatgtct acctcatcca aggaaggaac
    10201 catgtacgcc agaaattttt ctggattggc agtgtctgat ccagccacca catagctttt
    10261 caaaatggcc tgtggagtat gaggtcagag ctcgtcatca atatgaaaat aaggcaatca
    10321 accatagtta agattcagcc ccatatatca tatatcctat cctcattcaa gtacacttat
    10381 gtaagcaaca aaaacgatat agttgataca gtcatctttt ctctcaggaa caaagtatcc
    10441 ctatgataga gaccagagat aattggacca tactcatgga tatcggtaac tcataactca
    10501 cccgtgactc atgtgcatca cgaattgaag ggtccaactt gccaaagaat tcagaatcag
    10561 ctataggagc accatcaaaa ttcgctacca caaactgctc atcgtacctg tgtcaaaaaa
    10621 ggaaggggag agtcaaatgt aatagcatag tatgctacag gttcagtgaa aattattagt
    10681 ggatgtttca tccaaaaggc aatacactaa aaccctagca aagaaacatt tgaacaagag
    10741 ttggagcaga gaaaagggaa aaattaaaga cccaacatct ctcttaagtg ttgtatagtt
    10801 agaccttaac aaggctgcct attttcccac ccgattgatt acactatcct ataggaaaac
    10861 tattcgtgca tgtttccgac cacaggagat ccttcagaga gagccaacat aaagataatc
    10921 tgtaaagtgt cagctttcaa gctgtgtcta catgtaggac tattatgaga aaattttgcc
    10981 tgattcggta tttcagattt aagattgtct gcaaggggaa ataaagagca tccaacctat
    11041 caaaatatgg caacagtggt agaacttcaa ccggctgcaa gtttttattg gtggcatgaa
    11101 ctggtcgaga cttgcatgcc tcaaaggatg cttcaatatc cttgatttgt ctctctctag
    11161 agaccaaaga aaaataaacc attagacctt tttttttctc actttcctta gaaatacaca
    11221 tatttgagaa acaacacacc tattgttaag attgtgcaag atgttgatgc ctcctttcat
    11281 ctctcgcagt tcctttgctt gcttctccgt tagcgactac acaaggtaaa cataggaaat
    11341 tggaaactgt ctctagaagc tatacatggc aaaacaaatt tccaacaagc gagatggata
    11401 gatacctgtc ttgccgattc gttattaata gaagaaatgt attgtgtttt gacgagccaa
    11461 gacatgcctt tatcagtagg tctttctttc ctccggatgc catctttctt aataggtgta
    11521 acagcgtcat catcacgcaa gagttcttca tcttcagggg caagaggtgc cttgactttg
    11581 ggagggctga aaagagctac aacagtaagt atccccagta gatgaggtcg ctggcattaa
    11641 agacactaag actttattga gagcacatac ttgtacacac ttaggtcaag cagatccaag
    11701 ggtattccaa gatcaggctc gacaaagatt tttggtttcc ataatttctc cagggatgtg
    11761 atggtatact ttgtaaatct agtgggatca aaatagctgg caagtaagga cagtagaata
    11821 acacagaaat aaaatttggc aataatagag aaacatactg atctttatcc cgtttaatgg
    11881 tcataagctt aagctgtgca ctagggtcag ggagctcatt ccggaacctt gaagaagaag
    11941 atgaatatca gagtctaaca ataatatgta aaaaagattt aggtagtcgt ttgaagaata
    12001 ctgacttgag cttgcagatg aaggtggttg gtttctttaa cctattctcg accctgtcgg
    12061 tggtgagtag gggcgtcggc ttcttctcct cggtgtgtcc cttgggcatc tgagacttgt
    12121 gagaattttt catctgctgt ctatgttttt cgtcttgcct ttgtttctct aactccctct
    12181 tcttcctaag cctcctctct tcttcggtct cgattttctt agaatgagga agatgagacc
    12241 tgtggtggtg cttcgatgga tccggtttag cacgctcccg ccttccaacc tgcttggagg
    12301 cccctttctc gtggtcattg ggaccctggt gtcgaggagg atcgggaacg agcggaggag
    12361 gagctgaggg aggtggcgga ggaggaggag cttggagctg gttgaattga ggatagtggg
    12421 gcccctgctg atagtaagca tgcggaggag gaggcggcgg cggaggatat gagtacggtt
    12481 gatgcgacgg tggtggggga ggaacaggag ggggtaacga cggcggggga ggcggagggg
    12541 ccaaagaatt ttgagatggt ggctgaggaa gaggaggata cggcggccgg tacgacgcca
    12601 ttctcggtgt actttgtgat ctgaatcacc taaaccaact aaaacgagtc ttcttgatat
    12661 atattaatta tatagctccg gtcagactga tttttccttt ttactttaat ttgggcctca
    12721 ctagcggccc aataaccgct actatcatat gggttctgat aattacggct tcaatacaaa
    12781 caaaaacaat cttaagctca ccgttttatg aatcccaaat atgaacgaaa catgctttgt
    12841 accaccaagc tcatcgacgt gtgactgtgt gaggaacaca aaatatttat tttctacatt
    12901 gataattact caaacattat gtacagtata gcgaaccgag gtgtgtggtt aaacactcaa
    12961 cacgctggac caacttatga aaacctctta ccaggacttc acaagccgaa gatgttatgg
    13021 tcgttggcac tgaaaatagc accgccaaac tgcctggtgt tcaagtcgcc tccatcaagg
    13081 gcggtcccaa aacggtcaag ccactgggca ggactgggat tctctgcctc ttccacccac
    13141 tccgacatca tgtcaatgtc ctccagggca attgggtctg tctccaaggt tatcatcctc
    13201 cctaacttta gattctgatt cacgtacgcc aacttgttca atatttctct gtcaatcttg
    13261 tttcgccttt cccagtgcat ctgctggaat gtgctccatt ggcgttccaa gttgtaaccg
    13321 ctgcaaactt ggcttagtat tctgatggcc acccgctgca aaacaggtgc tgagtcacca
    13381 aactgctccc accagagacc tgcattacta agcaaggtga agggggcggc cgcggagcct
    13441 gcttttttgt acaaacttgt tgatgatccc gggcccgcgg taccgtcgac tgcagaattc
    13501 aattctgaga tttttcaaat cagtgcgcaa gacgtgacgt aagtatccga gtcagttttt
    13561 atttttctac taatttggtc gtttatttcg gcgtgtagga catggcaacc gggcctgaat
    13621 ttcgcgggta ttctgtttct attccaactt tttcttgatc cgcagccatt aacgactttt
    13681 gaatagatac gctgacacgc caagcctcgc tagtcaaaag tgtaccaaac aacgctttac
    13741 agcaagaacg gaatgcgcgt gacgctcgcg gtgacgccat ttcgcctttt cagaaatgga
    13801 taaatagcct tgcttcctat tatatcttcc caaattacca atacattaca ctagcatctg
    13861 aatttcataa ccaatctcga tacaccaaat cgaatcgatg agcccagaac gacgcccggc
    13921 cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg tcaaccacta
    13981 catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg agtggacgga
    14041 cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt
    14101 cgccggcatc gcctacgcgg gcccctggaa ggcacgcaac gcctacgact ggacggccga
    14161 gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca cgctctacac
    14221 ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg tcatcgggct
    14281 gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc gcggcatgct
    14341 gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc agctggactt
    14401 cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatttgac tgcagaatct
    14461 tggactccca tgttggcaaa ggcaaccaaa caaacaatga atgatccgct cctgcatatg
    14521 gggcggtttg agtatttcaa ctgccatttg ggctgaattg aagacatgct cctgtcagaa
    14581 attccgtgat cttactcaat attcagtaat ctcggccaat atcctaaatg tgcgtggctt
    14641 tatctgtctt tgtattgttt catcaattca tgtaacgttt gcttttctta tgaattttca
    14701 aataaattat cagatccgag ctcgaattaa ttcggcgtta attcagtaca ttaaaaacgt
    14761 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc
    14821 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg
    14881 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct
    14941 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg
    15001 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac
    15061 cgcggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt
    15121 gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc
    15181 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat
    15241 aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct
    15301 gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg
    15361 aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac
    15421 gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact
    15481 ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct
    15541 cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca
    15601 tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc
    15661 gcttagccga attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact
    15721 gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa
    15781 agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga
    15841 aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt
    15901 atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg
    15961 agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt
    16021 tactggatga attgttttag tacctagaat gcatgaccaa aatcccttaa cgctgagaga
    16081 tcccctcata atttccccaa agcgtaacca tgtgtgaata aattttgagc tagtagggtt
    16141 gcagccacga gtaagtcttc ccttgttatt gtgtagccag aatgccgcaa aacttccatg
    16201 cctaagcgaa ctgttgagag tacgtttcga tttctgactg tgttagcctg gaagtgcttg
    16261 tcccaacctt gtttctgagc atgaacgccc gcaagccaac atgttagttg aagcatcagg
    16321 gcgattagca gcatgatatc aaaacgctct gagctgctcg ttcggctatg gcgtaggcct
    16381 agtccgtagg caggactttt caagtctcgg aaggtttctt caatctgcat tcgcttcgaa
    16441 tagatattaa caagttgttt gggtgttcga atttcaacag gtaagttagt tgctagaatc
    16501 catggctcct ttgccgacgc tgagtagatt ttaggtgacg ggtggtgaca atgagtccgt
    16561 gtcgagcgct gattttttcg gcctttagag cgagatttat acaatagaat ttggcatgag
    16621 attggattgc ttttagtcag cctcttatag cctaaagtct ttgagtgact agatgacata
    16681 tcatgtaagt tgctgatagg tttccagttt tccgctccta ggtctgcata ttgtactttt
    16741 cctcttactc gacttaacca gtaccaaccc agcttctcaa cggatttata ccatggcact
    16801 ttaaagccag catcactgac aatgagcggt gtggtgttac tcggtagaat gctcgcaagg
    16861 tcggctagaa attggtcatg agctttcttt gaacattgct ctgaaagcgg gaacgctttc
    16921 tcataaagag taacagaacg accgtgtagt gcgactgaag ctcgcaatac cataagccgt
    16981 ttttgctcac ggatatcaga ccagtcaaca agtacaatgg gcatcgtatt gcccgaacag
    17041 ataaagctag catgccaacg gtatacagcg agtcgctctt tgtggaggtg acgattacct
    17101 aacaatcggt cgattcgttt gatgttatgt tttgttctcg ctttggttgg caggttacgg
    17161 ccaagttcgg taagagtgag agttttacag tcaagtaagg cgtggcaagc caacgttaag
    17221 ctgttgagtc gttttaagtg taattcgggg cagaattggt aaagagagtc gtgtaaaata
    17281 tcgagttcgc acattttgtt gtctgattat tgatttttgg cgaaaccatt tgatcatatg
    17341 acaagatgtg tatctacctt aacttaatga ttttgataaa aatcattagg ggattcatca
    17401 gcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    17461 cttc
//
