LOCUS       sequence-401419-       16847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express LHP1-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16847)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Li Z, Feng S, Kuo HY, Kan RL, 
            Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, 
            Wohlschlegel J, Jacobsen SE
  TITLE     A gene silencing screen uncovers diverse tools for targeted gene 
            repression in Arabidopsis.
  JOURNAL   Nat Plants. 2023 Mar 6. doi: 10.1038/s41477-023-01362-8.
  PUBMED    36879017
REFERENCE   2  (bases 1 to 16847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2023 Mar 6. doi: 10.1038/s41477-023-01362-8."
COMMENT     Sequence Label: pEG302-LHP1-ZF
FEATURES             Location/Qualifiers
     source          1..16847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5163..5185
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5177..5194
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5178..5194
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5217..5391
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5394..6359)
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(5397..6359)
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     terminator      complement(7820..8527)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(8607..8651)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(9177..9200)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(9207..9278)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    9337..9361
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12819..12843)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        12895..13275
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             13281..13832
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      13842..14094
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    14180..14204
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14629..15423
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     5041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     5161 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcagggatc
     5221 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
     5281 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
     5341 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa agcttatttt
     5401 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc atcctgctca
     5461 agtaataaag ccccctgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     5521 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgacagataa
     5581 ggtgccaatc cttcttgttc gaagagttcc aacgcagcac gtaattcacg tattagcatg
     5641 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     5701 ttaacgacac tctcttcaac acggcgcatt gccatgttga tcccggctcc aatgactatt
     5761 tgcgccgcat cgccagtttt gccagtcagc tccaccagaa tgcctgccag cttgcgatcc
     5821 tgcagataga ggtcattagg ccatttaaca cgaactttat ctgcacccag cttgcgtaat
     5881 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     5941 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccga
     6001 ccccggcgac cacggccagc ctgctggtat tctgcaatgc aagcatcgcc cgatttaagc
     6061 tctccgatac gatcaagaag gtactgattc gtggagtcaa tcactggcag cacggctaca
     6121 ctaccgccat ccagctgacc caatatctgt ttagcattaa gtaactggat aggctcaggc
     6181 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     6241 atgtgtttat taatagccgc ccggctcatt cccagcgttt cacccaactg ctcgccagag
     6301 tgaaattcac cgttcgctaa cagggcaatc aatttcagtg gcacggtgtt atccttcatc
     6361 tcgaggtcac acacgattgg ttgaatatca tcagcctcag ccatttttta tgagctgcaa
     6421 acacacaaaa agagttcaat acagtcaaat aaaccctgat tgaatcagaa atagctttct
     6481 gttcaacgta cgacactaca aatcctaaag tttcgaagaa tctatacaca aacttcatct
     6541 aaccttaaac agtgttcaga ttcaatctaa cattgacagt gttcagattc aatctaactt
     6601 caacagttca gatttcaatt cacatataca aatcatttcc caggtaacat gaacatggat
     6661 ctctccatca aggtcaagcc aaattctggt agctataatc gagctaactg atcggtcaac
     6721 ttagatcgat tctagtaaaa ccaaaagatt tagtggaggt tcacagatcc aaattaaacg
     6781 atatccagat ctcaaggaaa ttgagaaaac aagatcgaga tccagcaaat aaagtagatc
     6841 cagaaagttc ctattacctt ggaaagaaag agcggaagaa gatgagattg aggaagattc
     6901 aaaacggaga atgaatctcc tggattatct cttactttct ctcttagtct tcttccttgt
     6961 tcttctctgt caagtcgccg gagattcaaa acggctgatg aaagtgagga ggacaacgag
     7021 acaattcaaa gcggagagga aaatatatga atttatatag gcgggtttat ctcttacaac
     7081 tttattttcg gcctttcaaa aaaataatta aaatcgacag acacgaatca tttcgaccac
     7141 aggtaaagat aacgtgacct ggctgtcaga cagccttttc cctcgtgtta actaattttt
     7201 aaactaatta atcatctcag cccttggatt agttcttttg ctttgatggc ttcatgactg
     7261 tgacctgctc gatccgcgtg ttacatgaca gctccgtttt tttagtggtt aacttaaacc
     7321 gagtcaatcc aggcaacgtt agtcgtcgtc gtggttggct tgttcaatta gatttcatac
     7381 aattcaacgt aatttaattc gttttctatt agaattgtat cataattaat tcagaccgtg
     7441 aaagaaagtg tctttcatga tgtgtttatg gatatttata caataagata caatgtttca
     7501 tcatattcac tattcacgat tagtatgtac attaaataat ggctactact acatccgaac
     7561 tcgtcaaaac gattctgaat caattataca tatgctgact cttgcataca taaaaaatag
     7621 ttgtttaaat tttgtctaac taatgtttgg tataagtata atgttgagtt gagataccaa
     7681 ttacatcgag tctagccatt ttgtcgtgcc atattcgtca aaactttctt acataatgat
     7741 aacctagatc tagatgagat atgtatcaat gtatttgaga tcataattaa gttcgttcta
     7801 aattttgtcc ctgcaggtcc tgctgagcct cgacatgttg tcgcaaaatt cgccctggac
     7861 ccgcccaacg atttgtcgtc actgtcaagg tttgacctgc acttcatttg gggcccacat
     7921 acaccaaaaa aatgctgcat aattctcggg gcagcaagtc ggttacccgg ccgccgtgct
     7981 ggaccgggtt gaatggtgcc cgtaactttc ggtagagcgg acggccaata ctcaacttca
     8041 aggaatctca cccatgcgcg ccggcgggga accggagttc ccttcagtga gcgttattag
     8101 ttcgccgctc ggtgtgtcgt agatactagc ccctggggca cttttgaaat ttgaataaga
     8161 tttatgtaat cagtctttta ggtttgaccg gttctgccgc tttttttaaa attggatttg
     8221 taataataaa acgcaattgt ttgttattgt ggcgctctat catagatgtc gctataaacc
     8281 tattcagcac aatatattgt tttcatttta atattgtaca tataagtagt agggtacaat
     8341 cagtaaattg aacggagaat attattcata aaaatacgat agtaacgggt gatatattca
     8401 ttagaatgaa ccgaaaccgg cggtaaggat ctgagctaca catgctcagg ttttttacaa
     8461 cgtgcacaac agaattgaaa gcaaatatca tgcgatcata ggcgtctcgc atatctcatt
     8521 aaagcaggac tctagggact agtcccgggt cttaattaac tctctagagg cgcgccaagc
     8581 ttctactact aagatccacc agtatcctca tgccattcaa tcttctgagc ttcgaagata
     8641 tcgttaagtc caccagccat agatccctcg agtccggtgt gagtcctttg atgctcggtg
     8701 agcttatcac tcctagagaa agattttcca cactcagggc atttataagg tttttctcca
     8761 gtgtgtgtcc tttgatgagc cctaaggttt gctctctgag agaatgattt tccacactca
     8821 gggcatttat aaggtttttc accagtatgg gtcctttggt gcctaacaag gtttccagat
     8881 gttgagaatg attttccaca ctcagggcat ttataaggtt tttctccggt atgagtcctc
     8941 tgatgcctaa caagagatga agattgtgag aatgattttc cacactcagg gcatttataa
     9001 ggtttttcac cagtatgggt cctctgatgg gtggtaagat gatcactcct actgaatgat
     9061 tttccacact cagggcattt ataaggtttt tcaccggtat gggtcctttg gtgcctccta
     9121 agatctccag attgagagaa agattttcca cactcagggc atttataagg ctcgagaggt
     9181 ccttggaaaa gaacctcaag ggatcctttg tcgtcatcgt ctttgtagtc tttgtcgtca
     9241 tcgtctttgt agtctttgtc gtcatcgtct ttgtagtcgg taccaagctt ggcgcgcccg
     9301 tcccgggtct taattaactc tctagactca cctagcacca ctttgtacaa gaaagctggg
     9361 tcggcgcgcc cacccttagg cgttcgattg tacttgagat gttgctcata gaattcaatc
     9421 agctggtgtg ggaaaaacaa attagaaaag ctaataagaa acttccaaat gagtaagtgc
     9481 agaaaaaaaa agctataaag ataatcttac gagatgagga ttgtgagcct tgagaaatct
     9541 gttgtccacc aatgcttcct tcccatcaga cctatgagag taaccaaata agtacaattg
     9601 tacaaatatg aaatcaaatt acgagaaatg accaaagagc taaagagaca aacctcagcg
     9661 ctaaaaaggt caccaacact tcctggacat tgtctgatac agatgctgta aaactcattg
     9721 gtttaagtat cttcgtgatg tccaactcct caatcttggt tttctgagac aaattacaat
     9781 tttccatcac accaggatat tcattcccca tccttgcaat cctaccaaaa gagtctaatg
     9841 tagtcaaatc tggtgtcaga ttctgatctg taggtgctgt gtggttgtta cttgtggatc
     9901 catcttgttt gaatcttttc acagaaccag acttcctccg cttagcacct atgaaacgac
     9961 tgttcttatc aagctcctta ggataaacct tgagaaggcc attgggtctt acattatcac
    10021 cttcagaacc aatgcctcct ccttgagaac atcctgcacc gttactattg ttgactggtc
    10081 ctcgtagctc gttaagtgtt ggatcatatt ctttctcatt gtcaatcaaa cgaacttgtc
    10141 gggccatccc aacactccca ctgttggctt caacttggtt ggatacataa gcattcttcg
    10201 cttccacatc tctatttaat agactcgaac cgcttaggtc tagtggatca ggaatgtcag
    10261 gaaggctaga gttgttgaga gacgtagaag agtcagattt ctcagtagca tcatgtgatg
    10321 tagatgttaa acgttgcttc ttcttcatct gagaatgagg acctgcatat ttgcgcttcc
    10381 gtttcctacc aggctttcct ggcttcaaac tttgaaacat atgaaacaag aaagcaaatc
    10441 catacattaa accaagaaaa tccatgaaac aatcaattca aaagtatcac aaaactttta
    10501 tataagattc catttactta ccttccctca aaggcatcta taacatcagc aatagactgg
    10561 agattctcta aaggctccca tgtgttggca gtttcaggcc atccgcgcct ttcacaaccg
    10621 agtttataac ttatgtcaat ggaaaaacat atgtttaatc aagaacaaca aacttaaaaa
    10681 tgaaaccaag taagattaga aaaactcacc atttaattag atactgaacc ttgccctaca
    10741 aatacaaaaa aaaaaacagt accaaaatgc agaatcagaa tcattcaatc aatcaaatcc
    10801 aagaagatat caaacattaa agatcatttt cttcctacaa catataacaa ccacagaatc
    10861 aaaactttgg ttcgttataa actactctgt tacgatgatt actaactact aaagcagcca
    10921 ctttctcaca ccaatgtgta acctacgttt gatacttatc ttaaacctag aaaacctaaa
    10981 acctcaacta attatggtta acaaaaacaa accacatttg ctgagtgaag acgaaaaaag
    11041 gagcttactt ttcgaactct cttacgacga atagcttcaa tttcataaaa cccttcatca
    11101 agctttggcc tttcctcttg cccaccttct ccttctcctt cttcatcttc ctcatcacct
    11161 ccatcatctt catcctcgtc ctcttcttct tcttcatctc catcctccgt aggcctatca
    11221 tcaccaatct cgcgaagaat cgactctcca cttccacctc cacctccccc tccgccgtcg
    11281 tcactgccaa acccaccgtc tccactaatt ttccggcttt ctccgactgt ttccaccgcc
    11341 gtctccgcct caccagcttc gttcaacacc tggggtttct tcttaacagc accacttgcc
    11401 cctttcattt cctgagtatt cgagcctccc aaacctgaag gaggagggtg aatcaaggta
    11461 atcgcgttct cttctcttct tctccttttt agaaatcaag gattttggag gaagaagatg
    11521 aagaaagatt gaaacttttt ctctatttct gaggtttttg tttttgcagg aagaagacct
    11581 gacctgacct caaagaccag ttttttcact tttttggatt ctcttttttt tttttttttt
    11641 taatatcaca ataggaaaag ttgaatacag caaattctta acgtttataa tctaattaga
    11701 agagattatt tctaattact aatatgaaaa taataaatac tactgaagaa aatatgtgaa
    11761 ggtaaattaa atcgaaaaat atattaaact gaaaacattt tctttaaatt agaaataaaa
    11821 gaacatcgtg ctcttgctcc ttttacaagg aaatcaacta ggacattatt acaacttaaa
    11881 aaaaaatcaa aggagattga aaaatcagaa aataagtctc ataaactaag attctcactc
    11941 caagtctcca agtgcactgc aaaggcagac aacttagttg gatctcctaa catcatcatc
    12001 aataattttc agcaatatct catccaaacc aacagaaaag aaagtccttc tccaagataa
    12061 acttcatcga gattcagatt ctttcattcc aaagtatagt tatcttgata gtaactatgt
    12121 gattactgct cgagttttag cctcaacttt tggtgggcta aagttctatt ttcttcagtt
    12181 tagtgtgttt gttctatact ttgtccaacc aaaacttgtt tctttgtgat tagtacttgt
    12241 acactaaatg atgcatgttt tgatcgcttt gttaaatttg aattgttact ataaaatttg
    12301 cagttctttt tttgtttaaa cgttagaaaa ataataatta ctattattat ttaaaatggt
    12361 gatatgaaag aagtaaaaag gaagaaaaaa aacattcatt aggatcggtt cataaatagg
    12421 gggaaaaatg gaacgttttg acttcaacat aaataaatga gtattttatt tgatggtgag
    12481 cttatgaaag tttgtaacgt ttcctcgaaa tatatatgag aatatttttt ttgtaaaaat
    12541 tcacgattac aagcactcaa acaaatggaa tttttttccc cacacacaaa atatacatcc
    12601 attaaaaaat caagaaagaa gttaaaattt caaacctccc acaatttttt ttttgtttag
    12661 aaagagtata tccgaagaac aagagaacaa cctttttctc tgataaaaga aaaagttcat
    12721 ccttcttaac ctttttcaaa gttatgagag ttgcattaac ctaataatgc aatctcaaca
    12781 ccgatacttc attcttaggg tgaagggggc ggccgcggag cctgcttttt tgtacaaact
    12841 tgttgatgat cccgggcccg cggtaccgtc gactgcagaa ttcaattctg agatttttca
    12901 aatcagtgcg caagacgtga cgtaagtatc cgagtcagtt tttatttttc tactaatttg
    12961 gtcgtttatt tcggcgtgta ggacatggca accgggcctg aatttcgcgg gtattctgtt
    13021 tctattccaa ctttttcttg atccgcagcc attaacgact tttgaataga tacgctgaca
    13081 cgccaagcct cgctagtcaa aagtgtacca aacaacgctt tacagcaaga acggaatgcg
    13141 cgtgacgctc gcggtgacgc catttcgcct tttcagaaat ggataaatag ccttgcttcc
    13201 tattatatct tcccaaatta ccaatacatt acactagcat ctgaatttca taaccaatct
    13261 cgatacacca aatcgaatcg atgagcccag aacgacgccc ggccgacatc cgccgtgcca
    13321 ccgaggcgga catgccggcg gtctgcacca tcgtcaacca ctacatcgag acaagcacgg
    13381 tcaacttccg taccgagccg caggaaccgc aggagtggac ggacgacctc gtccgtctgc
    13441 gggagcgcta tccctggctc gtcgccgagg tggacggcga ggtcgccggc atcgcctacg
    13501 cgggcccctg gaaggcacgc aacgcctacg actggacggc cgagtcgacc gtgtacgtct
    13561 ccccccgcca ccagcggacg ggactgggct ccacgctcta cacccacctg ctgaagtccc
    13621 tggaggcaca gggcttcaag agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg
    13681 tgcgcatgca cgaggcgctc ggatatgccc cccgcggcat gctgcgggcg gccggcttca
    13741 agcacgggaa ctggcatgac gtgggtttct ggcagctgga cttcagcctg ccggtaccgc
    13801 cccgtccggt cctgcccgtc accgagattt gactgcagaa tcttggactc ccatgttggc
    13861 aaaggcaacc aaacaaacaa tgaatgatcc gctcctgcat atggggcggt ttgagtattt
    13921 caactgccat ttgggctgaa ttgaagacat gctcctgtca gaaattccgt gatcttactc
    13981 aatattcagt aatctcggcc aatatcctaa atgtgcgtgg ctttatctgt ctttgtattg
    14041 tttcatcaat tcatgtaacg tttgcttttc ttatgaattt tcaaataaat tatcagatcc
    14101 gagctcgaat taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa
    14161 gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc
    14221 tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg
    14281 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat
    14341 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc
    14401 atgttgattg taacgatgac agagcgttgc tgcctgtgat caccgcggtt tcaaaatcgg
    14461 ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg
    14521 gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc
    14581 ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg
    14641 agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa
    14701 ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa
    14761 atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg
    14821 ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc
    14881 tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat
    14941 gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc
    15001 atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat
    15061 tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca
    15121 tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt
    15181 gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt
    15241 ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc
    15301 gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta
    15361 ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt
    15421 tagtacctag aatgcatgac caaaatccct taacgctgag agatcccctc ataatttccc
    15481 caaagcgtaa ccatgtgtga ataaattttg agctagtagg gttgcagcca cgagtaagtc
    15541 ttcccttgtt attgtgtagc cagaatgccg caaaacttcc atgcctaagc gaactgttga
    15601 gagtacgttt cgatttctga ctgtgttagc ctggaagtgc ttgtcccaac cttgtttctg
    15661 agcatgaacg cccgcaagcc aacatgttag ttgaagcatc agggcgatta gcagcatgat
    15721 atcaaaacgc tctgagctgc tcgttcggct atggcgtagg cctagtccgt aggcaggact
    15781 tttcaagtct cggaaggttt cttcaatctg cattcgcttc gaatagatat taacaagttg
    15841 tttgggtgtt cgaatttcaa caggtaagtt agttgctaga atccatggct cctttgccga
    15901 cgctgagtag attttaggtg acgggtggtg acaatgagtc cgtgtcgagc gctgattttt
    15961 tcggccttta gagcgagatt tatacaatag aatttggcat gagattggat tgcttttagt
    16021 cagcctctta tagcctaaag tctttgagtg actagatgac atatcatgta agttgctgat
    16081 aggtttccag ttttccgctc ctaggtctgc atattgtact tttcctctta ctcgacttaa
    16141 ccagtaccaa cccagcttct caacggattt ataccatggc actttaaagc cagcatcact
    16201 gacaatgagc ggtgtggtgt tactcggtag aatgctcgca aggtcggcta gaaattggtc
    16261 atgagctttc tttgaacatt gctctgaaag cgggaacgct ttctcataaa gagtaacaga
    16321 acgaccgtgt agtgcgactg aagctcgcaa taccataagc cgtttttgct cacggatatc
    16381 agaccagtca acaagtacaa tgggcatcgt attgcccgaa cagataaagc tagcatgcca
    16441 acggtataca gcgagtcgct ctttgtggag gtgacgatta cctaacaatc ggtcgattcg
    16501 tttgatgtta tgttttgttc tcgctttggt tggcaggtta cggccaagtt cggtaagagt
    16561 gagagtttta cagtcaagta aggcgtggca agccaacgtt aagctgttga gtcgttttaa
    16621 gtgtaattcg gggcagaatt ggtaaagaga gtcgtgtaaa atatcgagtt cgcacatttt
    16681 gttgtctgat tattgatttt tggcgaaacc atttgatcat atgacaagat gtgtatctac
    16741 cttaacttaa tgattttgat aaaaatcatt aggggattca tcagccctta acgtgagttt
    16801 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
