LOCUS       sequence-401423-       18110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express JMJ18-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18110)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Li Z, Feng S, Kuo HY, Kan RL, 
            Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, 
            Wohlschlegel J, Jacobsen SE
  TITLE     A gene silencing screen uncovers diverse tools for targeted gene 
            repression in Arabidopsis.
  JOURNAL   Nat Plants. 2023 Mar 6. doi: 10.1038/s41477-023-01362-8.
  PUBMED    36879017
REFERENCE   2  (bases 1 to 18110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2023 Mar 6. doi: 10.1038/s41477-023-01362-8."
COMMENT     Sequence Label: pEG302-JMJ18-ZF
FEATURES             Location/Qualifiers
     source          1..18110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5163..5185
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5177..5194
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5178..5194
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5217..5391
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5394..6359)
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(5397..6359)
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     terminator      complement(7820..8527)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(8607..8651)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(9177..9200)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(9207..9278)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    9337..9361
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(14082..14106)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        14158..14538
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             14544..15095
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      15105..15357
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    15443..15467
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             15892..16686
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     5041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     5161 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcagggatc
     5221 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
     5281 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
     5341 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa agcttatttt
     5401 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc atcctgctca
     5461 agtaataaag ccccctgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     5521 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgacagataa
     5581 ggtgccaatc cttcttgttc gaagagttcc aacgcagcac gtaattcacg tattagcatg
     5641 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     5701 ttaacgacac tctcttcaac acggcgcatt gccatgttga tcccggctcc aatgactatt
     5761 tgcgccgcat cgccagtttt gccagtcagc tccaccagaa tgcctgccag cttgcgatcc
     5821 tgcagataga ggtcattagg ccatttaaca cgaactttat ctgcacccag cttgcgtaat
     5881 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     5941 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccga
     6001 ccccggcgac cacggccagc ctgctggtat tctgcaatgc aagcatcgcc cgatttaagc
     6061 tctccgatac gatcaagaag gtactgattc gtggagtcaa tcactggcag cacggctaca
     6121 ctaccgccat ccagctgacc caatatctgt ttagcattaa gtaactggat aggctcaggc
     6181 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     6241 atgtgtttat taatagccgc ccggctcatt cccagcgttt cacccaactg ctcgccagag
     6301 tgaaattcac cgttcgctaa cagggcaatc aatttcagtg gcacggtgtt atccttcatc
     6361 tcgaggtcac acacgattgg ttgaatatca tcagcctcag ccatttttta tgagctgcaa
     6421 acacacaaaa agagttcaat acagtcaaat aaaccctgat tgaatcagaa atagctttct
     6481 gttcaacgta cgacactaca aatcctaaag tttcgaagaa tctatacaca aacttcatct
     6541 aaccttaaac agtgttcaga ttcaatctaa cattgacagt gttcagattc aatctaactt
     6601 caacagttca gatttcaatt cacatataca aatcatttcc caggtaacat gaacatggat
     6661 ctctccatca aggtcaagcc aaattctggt agctataatc gagctaactg atcggtcaac
     6721 ttagatcgat tctagtaaaa ccaaaagatt tagtggaggt tcacagatcc aaattaaacg
     6781 atatccagat ctcaaggaaa ttgagaaaac aagatcgaga tccagcaaat aaagtagatc
     6841 cagaaagttc ctattacctt ggaaagaaag agcggaagaa gatgagattg aggaagattc
     6901 aaaacggaga atgaatctcc tggattatct cttactttct ctcttagtct tcttccttgt
     6961 tcttctctgt caagtcgccg gagattcaaa acggctgatg aaagtgagga ggacaacgag
     7021 acaattcaaa gcggagagga aaatatatga atttatatag gcgggtttat ctcttacaac
     7081 tttattttcg gcctttcaaa aaaataatta aaatcgacag acacgaatca tttcgaccac
     7141 aggtaaagat aacgtgacct ggctgtcaga cagccttttc cctcgtgtta actaattttt
     7201 aaactaatta atcatctcag cccttggatt agttcttttg ctttgatggc ttcatgactg
     7261 tgacctgctc gatccgcgtg ttacatgaca gctccgtttt tttagtggtt aacttaaacc
     7321 gagtcaatcc aggcaacgtt agtcgtcgtc gtggttggct tgttcaatta gatttcatac
     7381 aattcaacgt aatttaattc gttttctatt agaattgtat cataattaat tcagaccgtg
     7441 aaagaaagtg tctttcatga tgtgtttatg gatatttata caataagata caatgtttca
     7501 tcatattcac tattcacgat tagtatgtac attaaataat ggctactact acatccgaac
     7561 tcgtcaaaac gattctgaat caattataca tatgctgact cttgcataca taaaaaatag
     7621 ttgtttaaat tttgtctaac taatgtttgg tataagtata atgttgagtt gagataccaa
     7681 ttacatcgag tctagccatt ttgtcgtgcc atattcgtca aaactttctt acataatgat
     7741 aacctagatc tagatgagat atgtatcaat gtatttgaga tcataattaa gttcgttcta
     7801 aattttgtcc ctgcaggtcc tgctgagcct cgacatgttg tcgcaaaatt cgccctggac
     7861 ccgcccaacg atttgtcgtc actgtcaagg tttgacctgc acttcatttg gggcccacat
     7921 acaccaaaaa aatgctgcat aattctcggg gcagcaagtc ggttacccgg ccgccgtgct
     7981 ggaccgggtt gaatggtgcc cgtaactttc ggtagagcgg acggccaata ctcaacttca
     8041 aggaatctca cccatgcgcg ccggcgggga accggagttc ccttcagtga gcgttattag
     8101 ttcgccgctc ggtgtgtcgt agatactagc ccctggggca cttttgaaat ttgaataaga
     8161 tttatgtaat cagtctttta ggtttgaccg gttctgccgc tttttttaaa attggatttg
     8221 taataataaa acgcaattgt ttgttattgt ggcgctctat catagatgtc gctataaacc
     8281 tattcagcac aatatattgt tttcatttta atattgtaca tataagtagt agggtacaat
     8341 cagtaaattg aacggagaat attattcata aaaatacgat agtaacgggt gatatattca
     8401 ttagaatgaa ccgaaaccgg cggtaaggat ctgagctaca catgctcagg ttttttacaa
     8461 cgtgcacaac agaattgaaa gcaaatatca tgcgatcata ggcgtctcgc atatctcatt
     8521 aaagcaggac tctagggact agtcccgggt cttaattaac tctctagagg cgcgccaagc
     8581 ttctactact aagatccacc agtatcctca tgccattcaa tcttctgagc ttcgaagata
     8641 tcgttaagtc caccagccat agatccctcg agtccggtgt gagtcctttg atgctcggtg
     8701 agcttatcac tcctagagaa agattttcca cactcagggc atttataagg tttttctcca
     8761 gtgtgtgtcc tttgatgagc cctaaggttt gctctctgag agaatgattt tccacactca
     8821 gggcatttat aaggtttttc accagtatgg gtcctttggt gcctaacaag gtttccagat
     8881 gttgagaatg attttccaca ctcagggcat ttataaggtt tttctccggt atgagtcctc
     8941 tgatgcctaa caagagatga agattgtgag aatgattttc cacactcagg gcatttataa
     9001 ggtttttcac cagtatgggt cctctgatgg gtggtaagat gatcactcct actgaatgat
     9061 tttccacact cagggcattt ataaggtttt tcaccggtat gggtcctttg gtgcctccta
     9121 agatctccag attgagagaa agattttcca cactcagggc atttataagg ctcgagaggt
     9181 ccttggaaaa gaacctcaag ggatcctttg tcgtcatcgt ctttgtagtc tttgtcgtca
     9241 tcgtctttgt agtctttgtc gtcatcgtct ttgtagtcgg taccaagctt ggcgcgcccg
     9301 tcccgggtct taattaactc tctagactca cctagcacca ctttgtacaa gaaagctggg
     9361 tcggcgcgcc cacccttcat caaatctact ccgaaaagtt ttcctttggt taaacttgcg
     9421 gaacagtttg atgatatgaa gtgatctttt gagtctgatg aagtttggtt cttgtggttc
     9481 cagtactcaa ctagtcgatg gtttgggtca agagcttcaa tggcctgtag agaagcgtta
     9541 agttgtgtaa gagttaacat catgaatagc tttttaatga agaagaacaa ggcttggatt
     9601 tagatttctt acctgaacta tagagggtga gaggaaacca aacatttgaa gcccgttgat
     9661 actctcaaac tgtggtaaaa taggaagacc aagacttgtt gatgtatctt taactctccg
     9721 catcaccatt tcccagcatt gttgtgcaga gacattgaag aagctctcgt ctggagattc
     9781 ttccagagta accctgaaca atggacccat gagtcctgca tccaaaacct cagagatgta
     9841 attgctcatt cttgttggat caagaacatt gtagaacttg acacgactcc tgaatcctgt
     9901 taaagttgta aaacaaacat caacaaccat ttcagcaaat ttgatcaagt aacattaaca
     9961 aaagagcatt tcaaagatag taccttttgg gaatatagca tacttattgc accaaagctt
    10021 tccaaaaatc aagaacccga ggtttatagg ctcgaccgat acaccaaggt tctctgaggc
    10081 ggttaactcg ccgccactag tgctggcttc ttctttaacg tcttcttgat aatccatctc
    10141 taagtcaatg ttcagatcaa aagaaccttc ctttaacttc ttctcctcac tgataactga
    10201 actagtcttt gtctgatctt catcactatg ttcaatgcct aaaaccttgg aagcccatat
    10261 ctttaaatca tctgattccc cttccaatgc tctgaccaac gagcttaact catccattgt
    10321 gtaccggaga agaataaatc catctttcac atcacatgaa caaagatcat ctgcatgttt
    10381 aaggcacgcg tattcttcgg gagagcactt gcagccagaa gccgagagat gcaaatcata
    10441 aaagcatgag aaacattccc tttcacagtt tgaatcaaag tccttctcca tcttcttcaa
    10501 gcttgaagaa tccctaccaa gagctgtaat tctcccctct tccatttgta accgagcctg
    10561 tcaatatttt caaaacaaat gaaccaaagt aagcattaga aagtgaaaac tatattcagt
    10621 atcatgactc aagtagtatc tgtttttcgt tacttgtatc gcgttggtaa gtgtcccgtt
    10681 cttcccacag aaactcttcc atctcaaatt tgttgtattt tcctttccct cagaagctga
    10741 gagttcccaa agagccttca ccgcttcata agctgctcca agaagaagct tgtcgtgaga
    10801 cagagaagtc ttccttgtct ctttactata tagttccaca gcgttctgtc catgagccaa
    10861 ccaatcaacc ggagccacat taaccgcttc tgcgcagttg aaaccgcagt tgaatccagc
    10921 atgatacgcc ctcgggaatg tcaatacata ctcccctgaa ttctgcacga cccggtaagc
    10981 ctggactccc tcatctttca gtattgaagg agaaaactga gtaaccaggc catgtagtag
    11041 atcaggctgt tcttcgaaca agtcaggtaa atgtttcctc atcgccttct cgagagcggt
    11101 tgcattgctt cctggaacac cataccatac ttttggctcc ccaaagtgat gatagttaag
    11161 tgaatataaa tgatggtcct ccacatgctg ttcaagacaa agaaatatca catcaaacat
    11221 ccaaacatta gagaaaaaat gtttataata aaatcaaaga gtgaaagtgt acttacccaa
    11281 caaaatgatg aaaaacacat tcccacatat agccatggaa ctagaactcc ggatatgtca
    11341 caatcttcaa aagagagtac agagccagga agccgtggta agttattcaa gttccagcca
    11401 gataatgtgt actgctccat atcgctaccg gtaaacttct cggctctttt ataaaacccg
    11461 cttccaagca ccccattctc caagtcagct ccatagtaaa cctccacttc atctgttggt
    11521 tgctcaacta tccgccagta ttccccttct atatcatcca cagacggtgt ccattttact
    11581 atatctccac cagagtcttt cttttcaaag tacgagtctt taaaatgcag agcatatttc
    11641 tcaaactcat caagagtgaa gtctgaacca gaattgaaac caaacttctc ctcagcctcg
    11701 ggtgatgaag tcgattcagc gggagaagaa ccagatcgtc tcttagacga acccattctc
    11761 gagtttcttc tccgtttccg ctttctactc ttcggtttct tcttcatcgg ctcacggttc
    11821 tgaagcaggt ctacggtttg aatccgagta ggaaactttg tctgctccca tatactcttc
    11881 tccttaagcc ggcaaggagg cttccatgtc gatggtggga tgattcgaca gataccatac
    11941 ggctctgcta gtggacgtat cttctcgata tacgcaagtg gatctacaaa ctcctgataa
    12001 ccaataaaga gggaaattaa cgaacaagaa ctcaaaaaca tcaacaaaag atcatggaaa
    12061 caacaaagca attagtacct ctagtgatgg agtgaatacg ggagcgtcat tgataattgg
    12121 cctctgtgct tcatcaggta accatctagc aacaaccttt aacatataca aaaaaaaaaa
    12181 cattatgtaa gaacaaaact tgtttcaaga tttatataag tttttggatt ctgcatcaga
    12241 ttacatcata ttaccttccg atgacgtgga ctactaggtt gttccatgat agtgtctttg
    12301 tccttatcct tgtctggagg atgattcttc aaagacatat cctgtatatt aaaaacacac
    12361 ataaatttat ttttagagaa gaacaacaaa tatatgaata tgtgtttgat taaacagacc
    12421 tagaaactat aatcagcgtt acaaagatca agcataaaca acaaaactag tctataattc
    12481 aaagacgaag aggatctatg atcagtgatt aggtaatttg ccaaatcaag taaacaaacg
    12541 agatccggaa aacaaaagac tctatacaaa tctatgatga ataaaaacca aacatcaata
    12601 ctgtttttga ttcaaggtta cataatcttc agtaattaac cataaacaaa caagaaaaac
    12661 agtaattaat cgattgagac acgaattaaa catcgaaaaa ctcacctctt tgatctcaga
    12721 ttctaatgga ggattttcca tatgaattga aaaatcaata ctactcaaag cagattttgt
    12781 aagctaaaag ttttcttcga ttgagagcca atgaaactga gaaaaaaaaa aaagataaga
    12841 aaacgatgtt atcaacaaaa acaaatatcg atatatcaaa ataaagagaa gttaaaaaaa
    12901 aaaaaagttc ctaaccctaa ttgaattcac aaacagagga acaataacaa gaacatcaaa
    12961 aatcacagat atttaaattc atgaaaatca atcttttttt acctatcaaa ggagaaaaag
    13021 cttgaaagtt ttttggttct tttgttttgg cccaattaat gctataagaa ttgaattcgc
    13081 agttaaagtt agaaataccc aaatctggga aaatttaaga aagagattta attgacctag
    13141 tagtaacgac gaataagaag gaacacatta taaaacaaaa caaaccgaac ttaaccacga
    13201 taaggaactt aaaaatatta tttactacaa aattacaaaa gaggtttaaa aaaaaaaagg
    13261 aaaagtcgag aaggagatta ccacaaacga acggctgaga tttcgagaag aagaagaagt
    13321 gtatttgaga ttatctctga tgatgttata cgaatcttct ttttttttct atcaaatatt
    13381 atataatttt agataaaaaa aataaaaacc atgaaaattg aagtatgtga atatttaaaa
    13441 ataagagatt gagacagaga aaagacaaga gagtgatttg aagtaaagaa tctggagaaa
    13501 cgaggaagtt agagaacacc cgccaaagaa aacgcactct caagagtttt taacggatca
    13561 gcgtttgatc accacaccgt accgtacgat gatctaatat ttatgcggaa aatactgaat
    13621 taccctcagc tttctgttta ttttacggtt ctatgccatt tgttgttctt ccgtgatcgt
    13681 tcgtcgaacg tcgtctttgg ttttaggttt ctctccgcgt cggaaaagta aagagtttga
    13741 ccgtttcgtt tttttatcca aaacgcgtcg ttttaaagga aatggagatg aaagtttcat
    13801 tatttcgtcc ccgtaattta gtatttatta ttttttgtag ctatactttt gagatagatc
    13861 agaaagtaca gagaagtcaa attgtagaat aaaaataagg ttcttgaatc ttattagaaa
    13921 taaaataaat cacgacaaaa acatctccac atgagagttt gatcaactga ggaaaactaa
    13981 aagaaacaaa atgtgtaaaa caaaagaaag atgcaaattt gtggtacttt taatcaagtc
    14041 ttgtgttgta ctttcaatcg aggtgaaggg ggcggccgcg gagcctgctt ttttgtacaa
    14101 acttgttgat gatcccgggc ccgcggtacc gtcgactgca gaattcaatt ctgagatttt
    14161 tcaaatcagt gcgcaagacg tgacgtaagt atccgagtca gtttttattt ttctactaat
    14221 ttggtcgttt atttcggcgt gtaggacatg gcaaccgggc ctgaatttcg cgggtattct
    14281 gtttctattc caactttttc ttgatccgca gccattaacg acttttgaat agatacgctg
    14341 acacgccaag cctcgctagt caaaagtgta ccaaacaacg ctttacagca agaacggaat
    14401 gcgcgtgacg ctcgcggtga cgccatttcg ccttttcaga aatggataaa tagccttgct
    14461 tcctattata tcttcccaaa ttaccaatac attacactag catctgaatt tcataaccaa
    14521 tctcgataca ccaaatcgaa tcgatgagcc cagaacgacg cccggccgac atccgccgtg
    14581 ccaccgaggc ggacatgccg gcggtctgca ccatcgtcaa ccactacatc gagacaagca
    14641 cggtcaactt ccgtaccgag ccgcaggaac cgcaggagtg gacggacgac ctcgtccgtc
    14701 tgcgggagcg ctatccctgg ctcgtcgccg aggtggacgg cgaggtcgcc ggcatcgcct
    14761 acgcgggccc ctggaaggca cgcaacgcct acgactggac ggccgagtcg accgtgtacg
    14821 tctccccccg ccaccagcgg acgggactgg gctccacgct ctacacccac ctgctgaagt
    14881 ccctggaggc acagggcttc aagagcgtgg tcgctgtcat cgggctgccc aacgacccga
    14941 gcgtgcgcat gcacgaggcg ctcggatatg ccccccgcgg catgctgcgg gcggccggct
    15001 tcaagcacgg gaactggcat gacgtgggtt tctggcagct ggacttcagc ctgccggtac
    15061 cgccccgtcc ggtcctgccc gtcaccgaga tttgactgca gaatcttgga ctcccatgtt
    15121 ggcaaaggca accaaacaaa caatgaatga tccgctcctg catatggggc ggtttgagta
    15181 tttcaactgc catttgggct gaattgaaga catgctcctg tcagaaattc cgtgatctta
    15241 ctcaatattc agtaatctcg gccaatatcc taaatgtgcg tggctttatc tgtctttgta
    15301 ttgtttcatc aattcatgta acgtttgctt ttcttatgaa ttttcaaata aattatcaga
    15361 tccgagctcg aattaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat
    15421 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
    15481 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
    15541 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
    15601 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
    15661 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat
    15721 cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa aagctgtttt
    15781 ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt
    15841 agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa
    15901 atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg
    15961 gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta
    16021 aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg
    16081 atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat
    16141 ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa
    16201 gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac
    16261 tccatcgaca tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg
    16321 gattacttac tgaataacga tctggccgat gtggattgcg aaaactggga agaagacact
    16381 ccatttaaag atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa
    16441 cttgtctttt cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta
    16501 agtggcttta ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc
    16561 tgcgtccggt cgatcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac
    16621 ttactgggga tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg
    16681 ttttagtacc tagaatgcat gaccaaaatc ccttaacgct gagagatccc ctcataattt
    16741 ccccaaagcg taaccatgtg tgaataaatt ttgagctagt agggttgcag ccacgagtaa
    16801 gtcttccctt gttattgtgt agccagaatg ccgcaaaact tccatgccta agcgaactgt
    16861 tgagagtacg tttcgatttc tgactgtgtt agcctggaag tgcttgtccc aaccttgttt
    16921 ctgagcatga acgcccgcaa gccaacatgt tagttgaagc atcagggcga ttagcagcat
    16981 gatatcaaaa cgctctgagc tgctcgttcg gctatggcgt aggcctagtc cgtaggcagg
    17041 acttttcaag tctcggaagg tttcttcaat ctgcattcgc ttcgaataga tattaacaag
    17101 ttgtttgggt gttcgaattt caacaggtaa gttagttgct agaatccatg gctcctttgc
    17161 cgacgctgag tagattttag gtgacgggtg gtgacaatga gtccgtgtcg agcgctgatt
    17221 ttttcggcct ttagagcgag atttatacaa tagaatttgg catgagattg gattgctttt
    17281 agtcagcctc ttatagccta aagtctttga gtgactagat gacatatcat gtaagttgct
    17341 gataggtttc cagttttccg ctcctaggtc tgcatattgt acttttcctc ttactcgact
    17401 taaccagtac caacccagct tctcaacgga tttataccat ggcactttaa agccagcatc
    17461 actgacaatg agcggtgtgg tgttactcgg tagaatgctc gcaaggtcgg ctagaaattg
    17521 gtcatgagct ttctttgaac attgctctga aagcgggaac gctttctcat aaagagtaac
    17581 agaacgaccg tgtagtgcga ctgaagctcg caataccata agccgttttt gctcacggat
    17641 atcagaccag tcaacaagta caatgggcat cgtattgccc gaacagataa agctagcatg
    17701 ccaacggtat acagcgagtc gctctttgtg gaggtgacga ttacctaaca atcggtcgat
    17761 tcgtttgatg ttatgttttg ttctcgcttt ggttggcagg ttacggccaa gttcggtaag
    17821 agtgagagtt ttacagtcaa gtaaggcgtg gcaagccaac gttaagctgt tgagtcgttt
    17881 taagtgtaat tcggggcaga attggtaaag agagtcgtgt aaaatatcga gttcgcacat
    17941 tttgttgtct gattattgat ttttggcgaa accatttgat catatgacaa gatgtgtatc
    18001 taccttaact taatgatttt gataaaaatc attaggggat tcatcagccc ttaacgtgag
    18061 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
