LOCUS       sequence-401424-       17483 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HD2C-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17483)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Li Z, Feng S, Kuo HY, Kan RL, 
            Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, 
            Wohlschlegel J, Jacobsen SE
  TITLE     A gene silencing screen uncovers diverse tools for targeted gene 
            repression in Arabidopsis.
  JOURNAL   Nat Plants. 2023 Mar 6. doi: 10.1038/s41477-023-01362-8.
  PUBMED    36879017
REFERENCE   2  (bases 1 to 17483)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2023 Mar 6. doi: 10.1038/s41477-023-01362-8."
COMMENT     Sequence Label: pEG302-HD2C-ZF
FEATURES             Location/Qualifiers
     source          1..17483
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5163..5185
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5177..5194
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5178..5194
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5217..5391
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5394..6359)
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(5397..6359)
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     terminator      complement(7820..8527)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(8607..8651)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(9177..9200)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(9207..9278)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    9337..9361
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(13455..13479)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        13531..13911
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             13917..14468
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      14478..14730
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    14816..14840
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             15265..16059
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     5041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     5161 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcagggatc
     5221 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
     5281 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
     5341 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa agcttatttt
     5401 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc atcctgctca
     5461 agtaataaag ccccctgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     5521 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgacagataa
     5581 ggtgccaatc cttcttgttc gaagagttcc aacgcagcac gtaattcacg tattagcatg
     5641 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     5701 ttaacgacac tctcttcaac acggcgcatt gccatgttga tcccggctcc aatgactatt
     5761 tgcgccgcat cgccagtttt gccagtcagc tccaccagaa tgcctgccag cttgcgatcc
     5821 tgcagataga ggtcattagg ccatttaaca cgaactttat ctgcacccag cttgcgtaat
     5881 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     5941 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccga
     6001 ccccggcgac cacggccagc ctgctggtat tctgcaatgc aagcatcgcc cgatttaagc
     6061 tctccgatac gatcaagaag gtactgattc gtggagtcaa tcactggcag cacggctaca
     6121 ctaccgccat ccagctgacc caatatctgt ttagcattaa gtaactggat aggctcaggc
     6181 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     6241 atgtgtttat taatagccgc ccggctcatt cccagcgttt cacccaactg ctcgccagag
     6301 tgaaattcac cgttcgctaa cagggcaatc aatttcagtg gcacggtgtt atccttcatc
     6361 tcgaggtcac acacgattgg ttgaatatca tcagcctcag ccatttttta tgagctgcaa
     6421 acacacaaaa agagttcaat acagtcaaat aaaccctgat tgaatcagaa atagctttct
     6481 gttcaacgta cgacactaca aatcctaaag tttcgaagaa tctatacaca aacttcatct
     6541 aaccttaaac agtgttcaga ttcaatctaa cattgacagt gttcagattc aatctaactt
     6601 caacagttca gatttcaatt cacatataca aatcatttcc caggtaacat gaacatggat
     6661 ctctccatca aggtcaagcc aaattctggt agctataatc gagctaactg atcggtcaac
     6721 ttagatcgat tctagtaaaa ccaaaagatt tagtggaggt tcacagatcc aaattaaacg
     6781 atatccagat ctcaaggaaa ttgagaaaac aagatcgaga tccagcaaat aaagtagatc
     6841 cagaaagttc ctattacctt ggaaagaaag agcggaagaa gatgagattg aggaagattc
     6901 aaaacggaga atgaatctcc tggattatct cttactttct ctcttagtct tcttccttgt
     6961 tcttctctgt caagtcgccg gagattcaaa acggctgatg aaagtgagga ggacaacgag
     7021 acaattcaaa gcggagagga aaatatatga atttatatag gcgggtttat ctcttacaac
     7081 tttattttcg gcctttcaaa aaaataatta aaatcgacag acacgaatca tttcgaccac
     7141 aggtaaagat aacgtgacct ggctgtcaga cagccttttc cctcgtgtta actaattttt
     7201 aaactaatta atcatctcag cccttggatt agttcttttg ctttgatggc ttcatgactg
     7261 tgacctgctc gatccgcgtg ttacatgaca gctccgtttt tttagtggtt aacttaaacc
     7321 gagtcaatcc aggcaacgtt agtcgtcgtc gtggttggct tgttcaatta gatttcatac
     7381 aattcaacgt aatttaattc gttttctatt agaattgtat cataattaat tcagaccgtg
     7441 aaagaaagtg tctttcatga tgtgtttatg gatatttata caataagata caatgtttca
     7501 tcatattcac tattcacgat tagtatgtac attaaataat ggctactact acatccgaac
     7561 tcgtcaaaac gattctgaat caattataca tatgctgact cttgcataca taaaaaatag
     7621 ttgtttaaat tttgtctaac taatgtttgg tataagtata atgttgagtt gagataccaa
     7681 ttacatcgag tctagccatt ttgtcgtgcc atattcgtca aaactttctt acataatgat
     7741 aacctagatc tagatgagat atgtatcaat gtatttgaga tcataattaa gttcgttcta
     7801 aattttgtcc ctgcaggtcc tgctgagcct cgacatgttg tcgcaaaatt cgccctggac
     7861 ccgcccaacg atttgtcgtc actgtcaagg tttgacctgc acttcatttg gggcccacat
     7921 acaccaaaaa aatgctgcat aattctcggg gcagcaagtc ggttacccgg ccgccgtgct
     7981 ggaccgggtt gaatggtgcc cgtaactttc ggtagagcgg acggccaata ctcaacttca
     8041 aggaatctca cccatgcgcg ccggcgggga accggagttc ccttcagtga gcgttattag
     8101 ttcgccgctc ggtgtgtcgt agatactagc ccctggggca cttttgaaat ttgaataaga
     8161 tttatgtaat cagtctttta ggtttgaccg gttctgccgc tttttttaaa attggatttg
     8221 taataataaa acgcaattgt ttgttattgt ggcgctctat catagatgtc gctataaacc
     8281 tattcagcac aatatattgt tttcatttta atattgtaca tataagtagt agggtacaat
     8341 cagtaaattg aacggagaat attattcata aaaatacgat agtaacgggt gatatattca
     8401 ttagaatgaa ccgaaaccgg cggtaaggat ctgagctaca catgctcagg ttttttacaa
     8461 cgtgcacaac agaattgaaa gcaaatatca tgcgatcata ggcgtctcgc atatctcatt
     8521 aaagcaggac tctagggact agtcccgggt cttaattaac tctctagagg cgcgccaagc
     8581 ttctactact aagatccacc agtatcctca tgccattcaa tcttctgagc ttcgaagata
     8641 tcgttaagtc caccagccat agatccctcg agtccggtgt gagtcctttg atgctcggtg
     8701 agcttatcac tcctagagaa agattttcca cactcagggc atttataagg tttttctcca
     8761 gtgtgtgtcc tttgatgagc cctaaggttt gctctctgag agaatgattt tccacactca
     8821 gggcatttat aaggtttttc accagtatgg gtcctttggt gcctaacaag gtttccagat
     8881 gttgagaatg attttccaca ctcagggcat ttataaggtt tttctccggt atgagtcctc
     8941 tgatgcctaa caagagatga agattgtgag aatgattttc cacactcagg gcatttataa
     9001 ggtttttcac cagtatgggt cctctgatgg gtggtaagat gatcactcct actgaatgat
     9061 tttccacact cagggcattt ataaggtttt tcaccggtat gggtcctttg gtgcctccta
     9121 agatctccag attgagagaa agattttcca cactcagggc atttataagg ctcgagaggt
     9181 ccttggaaaa gaacctcaag ggatcctttg tcgtcatcgt ctttgtagtc tttgtcgtca
     9241 tcgtctttgt agtctttgtc gtcatcgtct ttgtagtcgg taccaagctt ggcgcgcccg
     9301 tcccgggtct taattaactc tctagactca cctagcacca ctttgtacaa gaaagctggg
     9361 tcggcgcgcc cacccttagc agctgcactg tgtttggcct ttgtgtgaga ctgcaatccc
     9421 atttccgaag taaaggttct ggtgcacgac ttgcacccaa acgctcctgc agacttcggt
     9481 gtctgctgct gcttcgatgt ctctccagtg ctgcctccac cagaattctt acccgcctgt
     9541 tttgagggat gaggagttgc aacatggaca tgcggtttct tcgaatctgc tttgtccgaa
     9601 tgttttttaa ttaaacaaat caatcttcct ttctatcgtt aaaataagta ttcaatgggg
     9661 agattggtaa aaaggattta atcacctgtt ttctgaggag ttacaaactt agccttcttg
     9721 ttggacgcag ggttcttgga ggagttgggt tctgcagacc tcttcttggg ttcttcaggc
     9781 ttttataaac caagaaaact tagaatcaga ttcgagcttg accacagaag cagagaaatt
     9841 gctcccaaag gaagataaga tagaacaaat ataggtttcc catcaacaag cacataaaag
     9901 agactgtcat gttaccttct ttggggtttc ctcttctgag gagtcatctt cttcgtcgtc
     9961 tgatgagtca tcttcctcac tgtcagattc agcagtaacc ttctcttcct gaaacaaagc
    10021 agccatgata aggattaaag aaatgtgaga atgggctaaa acacaatagg taatcaaggg
    10081 ttcatgtgaa aatgcaaata tagaagttac aaacatactt aagataactc tagtaccact
    10141 gtctataggt ttaatcaaca tgtcactgag cctaagtaaa attctataac aaattgagct
    10201 agtatatata gataaaagta cctcctcgtc tccagagttt tcactatcat catcatctga
    10261 agaatcttct tcactaccgt cagcgtcatc atcttgcttg gctttgacat cttcatttgg
    10321 caactgaaag ttcacctgtt tcgcagcaga tttcggagca gctggctcaa ccaaggccaa
    10381 ataaatttat aagagattga tgttcttaaa taaactaaaa taagacaact ggagacaaat
    10441 cattgcagga ctggatataa aagtccccat ttacctttga aaccagcagc ctcaagttga
    10501 tcatcaatca aatcctcagg ctcggtgaga tagagtcaag gaagtcaaaa acagagtaaa
    10561 gcaacaagtc cccaaaacat tttcatcaac aaagcttaaa gcgataacaa caaaaatgtt
    10621 caaatagtaa aggatatgga tcagatgcat caactttgta accagagaag aaaacgctcc
    10681 cattcttcca agtatgagac agcgcaaagt tcctttccaa gacaatctcc gtagacagct
    10741 gaggaaactt ctcatgagat agcgttccaa tgagaagctt atccgatcca acagtcacgt
    10801 aaagctggat tggttcggtt acattgttct tactctctcc caaagcaacc ttcaacaaaa
    10861 ataaaccaaa cgtcactgta aaatacattc ttataaatca aattaagctt gcaaattcaa
    10921 aagtacctga gagatgtgca cgagcctgtc tagcccagga tctaggtgaa gtggtttgcc
    10981 attcttaact tcaacacctg caaacgcata gaaagcatac aaatcagtaa ttctgcaaca
    11041 aaatccccaa atgaaacctc tatcggagtt aaaacctagc gagagagaaa acttttcagt
    11101 tttccccaat tttctacgaa gacgaacagt ccatacccta gattgtcaca tatactcact
    11161 attcccgttt ctcgtttagc taaacagaag attccataga aaaaacagta gaaaaacatg
    11221 gcaaaacgaa aaaaagcgat gaaaccagaa gctaaaaaaa aactaaatgc aggaaatgaa
    11281 gcgcagagaa cgaagaagaa gaaggagtaa gataggagat gttttttacc ccagaactcc
    11341 attgttgtgc gaggtagtgt gatcttgaag gcgacgatgc gtcgaaggag aaggagaagg
    11401 ttctagaaga agctgagagc gattcgtctc tcgagagcgg ttttagggcg tgttgatgtg
    11461 agaaggctaa aatgtccgct ggaatttatt aatcggagga agggatttta gggatcctct
    11521 gttacgcggt ccactcgaat atctccttta gttgggccat gattttaaaa ttagcccaac
    11581 aaaactaacg tttcaagccc atactttatt ttgacggctt tttttaacgg tgtcaggacg
    11641 gatcgtaacg gacttttaac actttatttg actgacggag ttttcgtcaa catgtggggt
    11701 ctatatattc atgtcttcat gacaatatct atctccattc ctcaaaataa gtcccacgga
    11761 taaattgagc ataatgtatc gcaaagtttt aatttttttc taatccaaag aaaaaccagt
    11821 taacaaaatc tgtgaagagt ttatatgata aaactaagta cggtccccga ctacgaataa
    11881 ttaaaattat taaatggata gttgcacgag tgagaattaa ggaacactat tattatatta
    11941 aacacaattc attttagtta gatctattcg gggcctctcg tgtttagatt tagaagagtt
    12001 ctcggcatga atcatgaaca ttttccaatg ctcttaaatt tatagtttac caatattata
    12061 ctccggtttt acatatcaca cataatttcg atttttttca acatatgtga tcataatcaa
    12121 ttgttaagtt ttaatttatg cgcataaaaa aatatatgtt tgtatagtag ttaaggagaa
    12181 tttttttttg ttatgtttta gcactttttg gattaatact agttttaaaa gtatgatttt
    12241 ttttttattt ttttttgtgc acaaatatga tcaaacccaa aagtttcttt acatagtagc
    12301 aactttatat tctcagcagc catggctaaa aaacgtaagt taatgacaaa aatgcgttga
    12361 gtgcaatgaa cctggcagta ttcgatgaac cttagcggat caaatgatat aaatatgcaa
    12421 tagaatttaa atatttattt attgtccaag ctgcgtggat agcttgatag ataaattaat
    12481 gacagcacat tgactaccca agctgcgttg ataagtactt gatcaaaatc tttacatttt
    12541 tatgaagtac tataatccaa agttaacttt taagcaaaag ataatttttt gtaatggttg
    12601 tgaaaaattc acttataata acacgaaaca taatgaagta ttattagttc atttatatat
    12661 aatacacaaa aaacataaag gcaataaaaa taaccaaatt aaaaaaaaat ggagaagtag
    12721 aaaagaaaac gaaaaaaaaa aaaaagagag agataagatg catgcatggt atagaagtcg
    12781 caggccgaag accagaaata cgaatgtgtt tgaatagaca aattaaggaa tataaagaca
    12841 gaagagccca tccattttcg catacacgaa tacatccccc tcgactccaa ttattttatt
    12901 atagtccatt ctctcacttt ctattctaaa tattgtggac gttataaagg ctttttattt
    12961 attttatgtc gagtttttta gactacgtag agccgaatga aaatttcatg tttaggtata
    13021 cgaaacttgg atccaatgtt acggattcag attggtgtaa aagatcaaat ttgatagtat
    13081 ttggatttga taggcaagat ggtttagtat tttacactgt gtatgttcct cttttagctt
    13141 tgcgttttct actttcacta cgatactact ttttatcttc caatttcagt tgcttatcac
    13201 caaaatatga aataccaaat taattgttta aacagtttta ttagcgatta aattagcaca
    13261 aaacatatga atagatatca tagtcgaata caaaaattag acaaataata atacactaaa
    13321 aaacaaacta aattggagaa ttgttttgac aaaaaataaa aaaaatgtca aagttccata
    13381 aaaaggagga caaaagagga atataacgaa attatcaaca gaaacgcacc gagtggtgaa
    13441 gggggcggcc gcggagcctg cttttttgta caaacttgtt gatgatcccg ggcccgcggt
    13501 accgtcgact gcagaattca attctgagat ttttcaaatc agtgcgcaag acgtgacgta
    13561 agtatccgag tcagttttta tttttctact aatttggtcg tttatttcgg cgtgtaggac
    13621 atggcaaccg ggcctgaatt tcgcgggtat tctgtttcta ttccaacttt ttcttgatcc
    13681 gcagccatta acgacttttg aatagatacg ctgacacgcc aagcctcgct agtcaaaagt
    13741 gtaccaaaca acgctttaca gcaagaacgg aatgcgcgtg acgctcgcgg tgacgccatt
    13801 tcgccttttc agaaatggat aaatagcctt gcttcctatt atatcttccc aaattaccaa
    13861 tacattacac tagcatctga atttcataac caatctcgat acaccaaatc gaatcgatga
    13921 gcccagaacg acgcccggcc gacatccgcc gtgccaccga ggcggacatg ccggcggtct
    13981 gcaccatcgt caaccactac atcgagacaa gcacggtcaa cttccgtacc gagccgcagg
    14041 aaccgcagga gtggacggac gacctcgtcc gtctgcggga gcgctatccc tggctcgtcg
    14101 ccgaggtgga cggcgaggtc gccggcatcg cctacgcggg cccctggaag gcacgcaacg
    14161 cctacgactg gacggccgag tcgaccgtgt acgtctcccc ccgccaccag cggacgggac
    14221 tgggctccac gctctacacc cacctgctga agtccctgga ggcacagggc ttcaagagcg
    14281 tggtcgctgt catcgggctg cccaacgacc cgagcgtgcg catgcacgag gcgctcggat
    14341 atgccccccg cggcatgctg cgggcggccg gcttcaagca cgggaactgg catgacgtgg
    14401 gtttctggca gctggacttc agcctgccgg taccgccccg tccggtcctg cccgtcaccg
    14461 agatttgact gcagaatctt ggactcccat gttggcaaag gcaaccaaac aaacaatgaa
    14521 tgatccgctc ctgcatatgg ggcggtttga gtatttcaac tgccatttgg gctgaattga
    14581 agacatgctc ctgtcagaaa ttccgtgatc ttactcaata ttcagtaatc tcggccaata
    14641 tcctaaatgt gcgtggcttt atctgtcttt gtattgtttc atcaattcat gtaacgtttg
    14701 cttttcttat gaattttcaa ataaattatc agatccgagc tcgaattaat tcggcgttaa
    14761 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
    14821 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
    14881 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
    14941 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
    15001 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
    15061 cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta tgttatacgc
    15121 caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag
    15181 gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg
    15241 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
    15301 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
    15361 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
    15421 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
    15481 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
    15541 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
    15601 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
    15661 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
    15721 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
    15781 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
    15841 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
    15901 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
    15961 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
    16021 aaaataaaat attatatttt actggatgaa ttgttttagt acctagaatg catgaccaaa
    16081 atcccttaac gctgagagat cccctcataa tttccccaaa gcgtaaccat gtgtgaataa
    16141 attttgagct agtagggttg cagccacgag taagtcttcc cttgttattg tgtagccaga
    16201 atgccgcaaa acttccatgc ctaagcgaac tgttgagagt acgtttcgat ttctgactgt
    16261 gttagcctgg aagtgcttgt cccaaccttg tttctgagca tgaacgcccg caagccaaca
    16321 tgttagttga agcatcaggg cgattagcag catgatatca aaacgctctg agctgctcgt
    16381 tcggctatgg cgtaggccta gtccgtaggc aggacttttc aagtctcgga aggtttcttc
    16441 aatctgcatt cgcttcgaat agatattaac aagttgtttg ggtgttcgaa tttcaacagg
    16501 taagttagtt gctagaatcc atggctcctt tgccgacgct gagtagattt taggtgacgg
    16561 gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg cctttagagc gagatttata
    16621 caatagaatt tggcatgaga ttggattgct tttagtcagc ctcttatagc ctaaagtctt
    16681 tgagtgacta gatgacatat catgtaagtt gctgataggt ttccagtttt ccgctcctag
    16741 gtctgcatat tgtacttttc ctcttactcg acttaaccag taccaaccca gcttctcaac
    16801 ggatttatac catggcactt taaagccagc atcactgaca atgagcggtg tggtgttact
    16861 cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga gctttctttg aacattgctc
    16921 tgaaagcggg aacgctttct cataaagagt aacagaacga ccgtgtagtg cgactgaagc
    16981 tcgcaatacc ataagccgtt tttgctcacg gatatcagac cagtcaacaa gtacaatggg
    17041 catcgtattg cccgaacaga taaagctagc atgccaacgg tatacagcga gtcgctcttt
    17101 gtggaggtga cgattaccta acaatcggtc gattcgtttg atgttatgtt ttgttctcgc
    17161 tttggttggc aggttacggc caagttcggt aagagtgaga gttttacagt caagtaaggc
    17221 gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt aattcggggc agaattggta
    17281 aagagagtcg tgtaaaatat cgagttcgca cattttgttg tctgattatt gatttttggc
    17341 gaaaccattt gatcatatga caagatgtgt atctacctta acttaatgat tttgataaaa
    17401 atcattaggg gattcatcag cccttaacgt gagttttcgt tccactgagc gtcagacccc
    17461 gtagaaaaga tcaaaggatc ttc
//
