LOCUS       sequence-401425-       15565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express TRB2-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15565)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Feng S, Shih YH, Liu M, Zhou 
            J, Richey JC, Ng C, Jami-Alahmadi Y, Wohlschlegel J, Wu K, Jacobsen 
            SE
  TITLE     Arabidopsis TRB proteins function in H3K4me3 demethylation by 
            recruiting JMJ14.
  JOURNAL   Nat Commun. 2023 Mar 28;14(1):1736. doi: 10.1038/s41467-023-37263-9.
  PUBMED    36977663
REFERENCE   2  (bases 1 to 15565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-03-28"; volume: "14(1):1736. doi"; pages: " 
            10.1038/s41467-023-37263-9"
COMMENT     Sequence Label: pEG302-TRB2-ZF
FEATURES             Location/Qualifiers
     source          1..15565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(111..363)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    1362..1386
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3562..3586)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3645..3716
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             3723..3746
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4272..4316
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     terminator      4396..5103
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             6564..7529
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             6564..7526
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     polyA_signal    7532..7706
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7729..7746)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7729..7745)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7738..7760)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7948..7972
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9273..9902
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10331..11404
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11470..11664
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11900..11922)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12008..12148
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12063..12082
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12163..12180)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12334..12922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12414..12433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14347..15141)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tgaattctgc agtcgacggt accgcgggcc cgggatcatc aacaagtttg tacaaaaaag
     1381 caggctccgc ggccgccccc ttcaccaaca catatttcaa tctagccatc aaaattttat
     1441 aaattacgct ttcggtctta ctattatatg aaaaatcttt aatagcccct aaactatctg
     1501 tgagatttaa caacgataaa ttttatagat acattatttt taaaaaatag tactagtata
     1561 ttttattcag cttgatccta ttcatttagt tggtaggttt tattgtgtaa tttgataaac
     1621 ttgaggcaaa tttgtgaatg tggagaaata tagggttaca tcttccttag cttgaataca
     1681 aaagaaaagc tcaagtttgt ttctcgtttc tatggatatt cttcttcctt atgctagcct
     1741 ctctggtctt cttcttcttc gttcgtttca gcgaatcaca tttgatctat gacacatcta
     1801 tacaaggaat tagctttcat catcaagtct ctctctcgaa aactctcctt tggtttcgtt
     1861 tgatctggca tgcttcttct cctccttcct cctttctgct tccttctctg aattttccat
     1921 ttttgttttt aattttctac gatcgcccca tttgaatttg gttgataatt tagctatcgc
     1981 tttagattga tggctgtatt tccaaattcc tgttgatttg cgacgttgtt aatgggcttt
     2041 gatcctcttt ttttggttcg agattgctcg aatcactcat ttttttgttg aattgagctt
     2101 tggggcgtat ctaatttctt tggatttgtg tagttacatt tttatgttgt ggctttgtgt
     2161 atctgctgga tttagatgac caaaattttg tattggataa gaaatgttca acaatggctt
     2221 tgatgtggtt aagtttcgta cgatttaaca gattctaatg ttagcgttca catctattgg
     2281 cagagaaaag aagaaattca aatattgata actgtggatg ggtgcaccaa agcagaagtg
     2341 gacaccggaa gaagaagcag ctcttaaagc tggagttctt aagcatggga ctgggaagtg
     2401 gcgtaccata ctctcggata ctgagtttag tttaatcctt aagtctcgct ctaatgttga
     2461 tcttaaggta atacaatata atatgctttt tggaggttgt gatcgcgtgt ttcggactaa
     2521 tcttgatagt tcaaggcata acaggacaaa tggagaaata taagcgtgac agctttatgg
     2581 ggttcaagga agaaggctaa acttgcgctt aagaggactc caccaggtac taaacaggat
     2641 gataacaaca cagctcttac cattgtggct ttgactaatg atgatgaacg tgcaaaacca
     2701 acttcccccg gaggttctgg tggtggatca ccacgcactt gtgcttctaa gagatcaatt
     2761 acaaggtttg gttccctgac ttatgcacca cataacgatt ttcttgagtt cataaagatt
     2821 tgatcttgcg ctattgtctg tttttgtagt ttggataaga tcatctttga ggcaattacc
     2881 aacttgaggg aactacgcgg ttctgacaga acatcaatct ttctgtatat agaggtattt
     2941 ctaccattgg gattacatgt ttggttgggg tttgttgcat acttacttag tagctgttaa
     3001 atttcaggag aatttcaaga ctccaccgaa tatgaaaagg catgtcgcag tacgattaaa
     3061 gcatttatca tcgaatggaa cattagttaa ggtaaacaca caacttttga ttcaagattc
     3121 atgatcacta acacttcatt actctcagaa ttttcaaaaa tacttacttg gtgatttctc
     3181 actttcagat aaagcacaag tacaggtttt cttctaattt tattcccgca ggtgcaagac
     3241 agaaggctcc tcaactcttt ctggaaggaa acaacaagaa agacccaaca aaacccgagg
     3301 agaacggtgc caacagtctc actaaattcc gagtagacgg tgaattatat atgataaaag
     3361 gcatgacagc tcaagaagct gcagaggctg cagcaagagc agttgcagag gcagaatttg
     3421 ctatcacaga ggctgaacaa gcagcaaaag aggcagaaag agcagaagca gaagctgaag
     3481 ctgctcagat atttgcaaag gcagccatga aagctttgaa gttcaggatc cgtaatcatc
     3541 cttggaaggg tgggcgcgcc gacccagctt tcttgtacaa agtggtgcta ggtgagtcta
     3601 gagagttaat taagacccgg gacgggcgcg ccaagcttgg taccgactac aaagacgatg
     3661 acgacaaaga ctacaaagac gatgacgaca aagactacaa agacgatgac gacaaaggat
     3721 cccttgaggt tcttttccaa ggacctctcg agccttataa atgccctgag tgtggaaaat
     3781 ctttctctca atctggagat cttaggaggc accaaaggac ccataccggt gaaaaacctt
     3841 ataaatgccc tgagtgtgga aaatcattca gtaggagtga tcatcttacc acccatcaga
     3901 ggacccatac tggtgaaaaa ccttataaat gccctgagtg tggaaaatca ttctcacaat
     3961 cttcatctct tgttaggcat cagaggactc ataccggaga aaaaccttat aaatgccctg
     4021 agtgtggaaa atcattctca acatctggaa accttgttag gcaccaaagg acccatactg
     4081 gtgaaaaacc ttataaatgc cctgagtgtg gaaaatcatt ctctcagaga gcaaacctta
     4141 gggctcatca aaggacacac actggagaaa aaccttataa atgccctgag tgtggaaaat
     4201 ctttctctag gagtgataag ctcaccgagc atcaaaggac tcacaccgga ctcgagggat
     4261 ctatggctgg tggacttaac gatatcttcg aagctcagaa gattgaatgg catgaggata
     4321 ctggtggatc ttagtagtag aagcttggcg cgcctctaga gagttaatta agacccggga
     4381 ctagtcccta gagtcctgct ttaatgagat atgcgagacg cctatgatcg catgatattt
     4441 gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac
     4501 cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata
     4561 atattctccg ttcaatttac tgattgtacc ctactactta tatgtacaat attaaaatga
     4621 aaacaatata ttgtgctgaa taggtttata gcgacatcta tgatagagcg ccacaataac
     4681 aaacaattgc gttttattat tacaaatcca attttaaaaa aagcggcaga accggtcaaa
     4741 cctaaaagac tgattacata aatcttattc aaatttcaaa agtgccccag gggctagtat
     4801 ctacgacaca ccgagcggcg aactaataac gctcactgaa gggaactccg gttccccgcc
     4861 ggcgcgcatg ggtgagattc cttgaagttg agtattggcc gtccgctcta ccgaaagtta
     4921 cgggcaccat tcaacccggt ccagcacggc ggccgggtaa ccgacttgct gccccgagaa
     4981 ttatgcagca tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca
     5041 gtgacgacaa atcgttgggc gggtccaggg cgaattttgc gacaacatgt cgaggctcag
     5101 caggacctgc agggacaaaa tttagaacga acttaattat gatctcaaat acattgatac
     5161 atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat atggcacgac
     5221 aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta taccaaacat
     5281 tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca tatgtataat
     5341 tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta atgtacatac
     5401 taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata tccataaaca
     5461 catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt ctaatagaaa
     5521 acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc acgacgacga
     5581 ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga gctgtcatgt
     5641 aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa ctaatccaag
     5701 ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc tgtctgacag
     5761 ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat tttaattatt
     5821 tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa attcatatat
     5881 tttcctctcc gctttgaatt gtctcgttgt cctcctcact ttcatcagcc gttttgaatc
     5941 tccggcgact tgacagagaa gaacaaggaa gaagactaag agagaaagta agagataatc
     6001 caggagattc attctccgtt ttgaatcttc ctcaatctca tcttcttccg ctctttcttt
     6061 ccaaggtaat aggaactttc tggatctact ttatttgctg gatctcgatc ttgttttctc
     6121 aatttccttg agatctggat atcgtttaat ttggatctgt gaacctccac taaatctttt
     6181 ggttttacta gaatcgatct aagttgaccg atcagttagc tcgattatag ctaccagaat
     6241 ttggcttgac cttgatggag agatccatgt tcatgttacc tgggaaatga tttgtatatg
     6301 tgaattgaaa tctgaactgt tgaagttaga ttgaatctga acactgtcaa tgttagattg
     6361 aatctgaaca ctgtttaagg ttagatgaag tttgtgtata gattcttcga aactttagga
     6421 tttgtagtgt cgtacgttga acagaaagct atttctgatt caatcagggt ttatttgact
     6481 gtattgaact ctttttgtgt gtttgcagct cataaaaaat ggctgaggct gatgatattc
     6541 aaccaatcgt gtgtgacctc gagatgaagg ataacaccgt gccactgaaa ttgattgccc
     6601 tgttagcgaa cggtgaattt cactctggcg agcagttggg tgaaacgctg ggaatgagcc
     6661 gggcggctat taataaacac attcagacac tgcgtgactg gggcgttgat gtctttaccg
     6721 ttccgggtaa aggatacagc ctgcctgagc ctatccagtt acttaatgct aaacagatat
     6781 tgggtcagct ggatggcggt agtgtagccg tgctgccagt gattgactcc acgaatcagt
     6841 accttcttga tcgtatcgga gagcttaaat cgggcgatgc ttgcattgca gaataccagc
     6901 aggctggccg tggtcgccgg ggtcggaaat ggttttcgcc ttttggcgca aacttatatt
     6961 tgtcgatgtt ctggcgtctg gaacaaggcc cggcggcggc gattggttta agtctggtta
     7021 tcggtatcgt gatggcggaa gtattacgca agctgggtgc agataaagtt cgtgttaaat
     7081 ggcctaatga cctctatctg caggatcgca agctggcagg cattctggtg gagctgactg
     7141 gcaaaactgg cgatgcggcg caaatagtca ttggagccgg gatcaacatg gcaatgcgcc
     7201 gtgttgaaga gagtgtcgtt aatcaggggt ggatcacgct gcaggaagcg gggatcaatc
     7261 tcgatcgtaa tacgttggcg gccatgctaa tacgtgaatt acgtgctgcg ttggaactct
     7321 tcgaacaaga aggattggca ccttatctgt cgcgctggga aaagctggat aattttatta
     7381 atcgcccagt gaaacttatc attggtgata aagaaatatt tggcatttca cgcggaatag
     7441 acaaacaggg ggctttatta cttgagcagg atggaataat aaaaccctgg atgggcggtg
     7501 aaatatccct gcgtagtgca gaaaaataag ctttctccat aataatgtgt gagtagttcc
     7561 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
     7621 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
     7681 caaaatccag tactaaaatc cagatccctg caggcatgca agcttggcac tggccgtcgt
     7741 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     7801 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     7861 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc
     7921 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
     7981 aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg
     8041 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
     8101 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa
     8161 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     8221 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     8281 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     8341 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     8401 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     8461 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
     8521 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
     8581 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
     8641 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
     8701 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
     8761 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
     8821 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
     8881 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
     8941 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
     9001 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     9061 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     9121 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     9181 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
     9241 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     9301 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
     9361 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     9421 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     9481 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     9541 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     9601 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     9661 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     9721 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     9781 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     9841 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     9901 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     9961 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
    10021 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
    10081 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
    10141 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
    10201 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
    10261 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg
    10321 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
    10381 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
    10441 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
    10501 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
    10561 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
    10621 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
    10681 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
    10741 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
    10801 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
    10861 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
    10921 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
    10981 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
    11041 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
    11101 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
    11161 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
    11221 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
    11281 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
    11341 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
    11401 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
    11461 tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
    11521 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
    11581 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
    11641 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
    11701 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
    11761 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
    11821 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
    11881 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    11941 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    12001 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
    12061 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
    12121 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
    12181 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    12241 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    12301 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    12361 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    12421 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    12481 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    12541 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    12601 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    12661 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    12721 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
    12781 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    12841 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    12901 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    12961 gtggaacgaa aactcacgtt aagggctgat gaatccccta atgattttta tcaaaatcat
    13021 taagttaagg tagatacaca tcttgtcata tgatcaaatg gtttcgccaa aaatcaataa
    13081 tcagacaaca aaatgtgcga actcgatatt ttacacgact ctctttacca attctgcccc
    13141 gaattacact taaaacgact caacagctta acgttggctt gccacgcctt acttgactgt
    13201 aaaactctca ctcttaccga acttggccgt aacctgccaa ccaaagcgag aacaaaacat
    13261 aacatcaaac gaatcgaccg attgttaggt aatcgtcacc tccacaaaga gcgactcgct
    13321 gtataccgtt ggcatgctag ctttatctgt tcgggcaata cgatgcccat tgtacttgtt
    13381 gactggtctg atatccgtga gcaaaaacgg cttatggtat tgcgagcttc agtcgcacta
    13441 cacggtcgtt ctgttactct ttatgagaaa gcgttcccgc tttcagagca atgttcaaag
    13501 aaagctcatg accaatttct agccgacctt gcgagcattc taccgagtaa caccacaccg
    13561 ctcattgtca gtgatgctgg ctttaaagtg ccatggtata aatccgttga gaagctgggt
    13621 tggtactggt taagtcgagt aagaggaaaa gtacaatatg cagacctagg agcggaaaac
    13681 tggaaaccta tcagcaactt acatgatatg tcatctagtc actcaaagac tttaggctat
    13741 aagaggctga ctaaaagcaa tccaatctca tgccaaattc tattgtataa atctcgctct
    13801 aaaggccgaa aaaatcagcg ctcgacacgg actcattgtc accacccgtc acctaaaatc
    13861 tactcagcgt cggcaaagga gccatggatt ctagcaacta acttacctgt tgaaattcga
    13921 acacccaaac aacttgttaa tatctattcg aagcgaatgc agattgaaga aaccttccga
    13981 gacttgaaaa gtcctgccta cggactaggc ctacgccata gccgaacgag cagctcagag
    14041 cgttttgata tcatgctgct aatcgccctg atgcttcaac taacatgttg gcttgcgggc
    14101 gttcatgctc agaaacaagg ttgggacaag cacttccagg ctaacacagt cagaaatcga
    14161 aacgtactct caacagttcg cttaggcatg gaagttttgc ggcattctgg ctacacaata
    14221 acaagggaag acttactcgt ggctgcaacc ctactagctc aaaatttatt cacacatggt
    14281 tacgctttgg ggaaattatg aggggatctc tcagcgttaa gggattttgg tcatgcattc
    14341 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
    14401 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
    14461 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
    14521 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
    14581 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
    14641 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
    14701 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
    14761 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
    14821 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
    14881 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
    14941 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
    15001 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
    15061 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
    15121 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
    15181 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
    15241 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
    15301 tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac
    15361 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
    15421 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
    15481 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
    15541 ggtcggggag ctgttggctg gctgg
//
