LOCUS       sequence-401426-       19817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express JMJ14-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19817)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Li Z, Feng S, Kuo HY, Kan RL, 
            Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, 
            Wohlschlegel J, Jacobsen SE
  TITLE     A gene silencing screen uncovers diverse tools for targeted gene 
            repression in Arabidopsis.
  JOURNAL   Nat Plants. 2023 Mar 6. doi: 10.1038/s41477-023-01362-8.
  PUBMED    36879017
REFERENCE   2  (bases 1 to 19817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2023 Mar 6. doi: 10.1038/s41477-023-01362-8."
COMMENT     Sequence Label: pEG302-JMJ14-ZF
FEATURES             Location/Qualifiers
     source          1..19817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5163..5185
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5177..5194
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5178..5194
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5217..5391
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5394..6359)
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             complement(5397..6359)
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     terminator      complement(7820..8527)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    8579..8603
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(8643..8687)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(9084..9104)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9120..9143)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(9150..9221)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             14554..14565
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(15789..15813)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        15865..16245
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             16251..16802
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      16812..17064
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    17150..17174
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             17599..18393
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     5041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     5161 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcagggatc
     5221 tggattttag tactggattt tggttttagg aattagaaat tttattgata gaagtatttt
     5281 acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg aaaccctata
     5341 ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa agcttatttt
     5401 tctgcactac gcagggatat ttcaccgccc atccagggtt ttattattcc atcctgctca
     5461 agtaataaag ccccctgttt gtctattccg cgtgaaatgc caaatatttc tttatcacca
     5521 atgataagtt tcactgggcg attaataaaa ttatccagct tttcccagcg cgacagataa
     5581 ggtgccaatc cttcttgttc gaagagttcc aacgcagcac gtaattcacg tattagcatg
     5641 gccgccaacg tattacgatc gagattgatc cccgcttcct gcagcgtgat ccacccctga
     5701 ttaacgacac tctcttcaac acggcgcatt gccatgttga tcccggctcc aatgactatt
     5761 tgcgccgcat cgccagtttt gccagtcagc tccaccagaa tgcctgccag cttgcgatcc
     5821 tgcagataga ggtcattagg ccatttaaca cgaactttat ctgcacccag cttgcgtaat
     5881 acttccgcca tcacgatacc gataaccaga cttaaaccaa tcgccgccgc cgggccttgt
     5941 tccagacgcc agaacatcga caaatataag tttgcgccaa aaggcgaaaa ccatttccga
     6001 ccccggcgac cacggccagc ctgctggtat tctgcaatgc aagcatcgcc cgatttaagc
     6061 tctccgatac gatcaagaag gtactgattc gtggagtcaa tcactggcag cacggctaca
     6121 ctaccgccat ccagctgacc caatatctgt ttagcattaa gtaactggat aggctcaggc
     6181 aggctgtatc ctttacccgg aacggtaaag acatcaacgc cccagtcacg cagtgtctga
     6241 atgtgtttat taatagccgc ccggctcatt cccagcgttt cacccaactg ctcgccagag
     6301 tgaaattcac cgttcgctaa cagggcaatc aatttcagtg gcacggtgtt atccttcatc
     6361 tcgaggtcac acacgattgg ttgaatatca tcagcctcag ccatttttta tgagctgcaa
     6421 acacacaaaa agagttcaat acagtcaaat aaaccctgat tgaatcagaa atagctttct
     6481 gttcaacgta cgacactaca aatcctaaag tttcgaagaa tctatacaca aacttcatct
     6541 aaccttaaac agtgttcaga ttcaatctaa cattgacagt gttcagattc aatctaactt
     6601 caacagttca gatttcaatt cacatataca aatcatttcc caggtaacat gaacatggat
     6661 ctctccatca aggtcaagcc aaattctggt agctataatc gagctaactg atcggtcaac
     6721 ttagatcgat tctagtaaaa ccaaaagatt tagtggaggt tcacagatcc aaattaaacg
     6781 atatccagat ctcaaggaaa ttgagaaaac aagatcgaga tccagcaaat aaagtagatc
     6841 cagaaagttc ctattacctt ggaaagaaag agcggaagaa gatgagattg aggaagattc
     6901 aaaacggaga atgaatctcc tggattatct cttactttct ctcttagtct tcttccttgt
     6961 tcttctctgt caagtcgccg gagattcaaa acggctgatg aaagtgagga ggacaacgag
     7021 acaattcaaa gcggagagga aaatatatga atttatatag gcgggtttat ctcttacaac
     7081 tttattttcg gcctttcaaa aaaataatta aaatcgacag acacgaatca tttcgaccac
     7141 aggtaaagat aacgtgacct ggctgtcaga cagccttttc cctcgtgtta actaattttt
     7201 aaactaatta atcatctcag cccttggatt agttcttttg ctttgatggc ttcatgactg
     7261 tgacctgctc gatccgcgtg ttacatgaca gctccgtttt tttagtggtt aacttaaacc
     7321 gagtcaatcc aggcaacgtt agtcgtcgtc gtggttggct tgttcaatta gatttcatac
     7381 aattcaacgt aatttaattc gttttctatt agaattgtat cataattaat tcagaccgtg
     7441 aaagaaagtg tctttcatga tgtgtttatg gatatttata caataagata caatgtttca
     7501 tcatattcac tattcacgat tagtatgtac attaaataat ggctactact acatccgaac
     7561 tcgtcaaaac gattctgaat caattataca tatgctgact cttgcataca taaaaaatag
     7621 ttgtttaaat tttgtctaac taatgtttgg tataagtata atgttgagtt gagataccaa
     7681 ttacatcgag tctagccatt ttgtcgtgcc atattcgtca aaactttctt acataatgat
     7741 aacctagatc tagatgagat atgtatcaat gtatttgaga tcataattaa gttcgttcta
     7801 aattttgtcc ctgcaggtcc tgctgagcct cgacatgttg tcgcaaaatt cgccctggac
     7861 ccgcccaacg atttgtcgtc actgtcaagg tttgacctgc acttcatttg gggcccacat
     7921 acaccaaaaa aatgctgcat aattctcggg gcagcaagtc ggttacccgg ccgccgtgct
     7981 ggaccgggtt gaatggtgcc cgtaactttc ggtagagcgg acggccaata ctcaacttca
     8041 aggaatctca cccatgcgcg ccggcgggga accggagttc ccttcagtga gcgttattag
     8101 ttcgccgctc ggtgtgtcgt agatactagc ccctggggca cttttgaaat ttgaataaga
     8161 tttatgtaat cagtctttta ggtttgaccg gttctgccgc tttttttaaa attggatttg
     8221 taataataaa acgcaattgt ttgttattgt ggcgctctat catagatgtc gctataaacc
     8281 tattcagcac aatatattgt tttcatttta atattgtaca tataagtagt agggtacaat
     8341 cagtaaattg aacggagaat attattcata aaaatacgat agtaacgggt gatatattca
     8401 ttagaatgaa ccgaaaccgg cggtaaggat ctgagctaca catgctcagg ttttttacaa
     8461 cgtgcacaac agaattgaaa gcaaatatca tgcgatcata ggcgtctcgc atatctcatt
     8521 aaagcaggac tctagggact agtcccgggt cttaattaac tctctagact cacctagcac
     8581 cactttgtac aagaaagctg ggtcggcgcg ccaagcttct actactaaga tccaccagta
     8641 tcctcatgcc attcaatctt ctgagcttcg aagatatcgt taagtccacc agccatagat
     8701 ccctcgaggg cctggcccga ggttttcttc ccgggctttt ctccggaacc gccacccagc
     8761 tcgccggtat gcgtacgctg atgcctcacc aggctaccgg aggtgctaaa cgacttcccg
     8821 cattccgggc aggcgtaggg tttctcgccg gtatgggtcc tctggtgacg cacaaggtca
     8881 tcgctacggc taaagctttt accgcattcg ggacacgcat acggtttctc gccggtatgc
     8941 gtacgctgat gcctcaccag gctaccggag gtgctaaacg acttcccgca ttccgggcag
     9001 gcgtagggtt tctcgccggt gggttcgagg gccgcctggg cctcgagatc gatatcctgc
     9061 aggatcccgg tacctgctag ctcgacctta cgcttctttt tcggaggctc aagctcgaga
     9121 ggtccttgga aaagaacctc aagggatcct ttgtcgtcat cgtctttgta gtctttgtcg
     9181 tcatcgtctt tgtagtcttt gtcgtcatcg tctttgtagt cggtaccagc ttggcgcgcc
     9241 cacccttagg acttatctcc atcttatcaa ccaaagtaga gagctcttcc ttgagctcgg
     9301 tgttgcgagt ctcaccacac agaagtccat gcatcattac taattcttca ggtgtcgcct
     9361 tcttcaacag tccacgcaga agccttgtgt cccggtccaa agagggatct gaagcccctc
     9421 ctttctctgt atcatccttt tctccttcct ttattggttc agcaccaaac agtttcactg
     9481 ctttttggtt ccaatactcc tccaattgat gctttggatc aagagcctca accacctgtg
     9541 cacaatggtc aactttcagt ccaaaacgct aaaaagtaca tatggaaatt tggtcatgta
     9601 ggcgagtaag agagatgctc aaggggactg aaacagaaga tgggagcttc tctcatccag
     9661 caaagtagaa gaaaaaaaaa aaaaaaaaaa ctttccaccg caagaagatt caggatatca
     9721 tagttttatg gctatgcatg agaaattgat tgtcacctta attacgtgcg gggagagaaa
     9781 tccaaacatt tcaagcccat ttatactctc caaaggttgt aaagaggtca aagagctaac
     9841 tggttgatca catttcttga tgatttcgag tttgagcctt tgtgtcacca tttgccagca
     9901 cttttcagca gatacattcg agaaattctc agtggggtaa tcttctactg agacctggaa
     9961 ataatactga ttgatcagta acttaccacc ttcatgtgtt ctgcatgata aatagatatt
    10021 cagttatatt ctcgaaacca ctacgtagtt agtgtaccct gaacaatgga ccaagaagcc
    10081 ctgcatccag aacctctgag atgtagttgg ttaggtttgt tgggtcaagc acacttaaga
    10141 acttaacacg gctcttgaac cctgtaacat acaatcaaaa gcaaaagtct cagaagacat
    10201 aaaaaataaa atactaaagt ataaagctca tccaaaattc tcaattcatc accttttggg
    10261 tatatggcct gtttactgca ccacagcttt ttaacaacga gagatccaga actcaaaagc
    10321 tcaacagaga gacttaacct attggttgca gcattttggt ccttccctcc taaacaacca
    10381 tccactttct tcgccagctc gctacaccct acatctgaaa ctgaatgtgg gttttgagat
    10441 tcaaccgaaa tcttcttctc atcaaactta cctcccacac caagttttac atccgtgaca
    10501 gctgacttat cagtgatccc atgtacatga tttctttcgc tttcatgacc atggcgattt
    10561 acatctgaat caccatcttg tttcaactga acttccttgt ttgaggttag atcactttgt
    10621 aagcgttcag acactaactg aagattgttt tgttctcgct gctgtacttt tgatgaacca
    10681 cggctcttta tacatggggc agacttgagg taagcatatt ctcttgctct tggatgctgt
    10741 gaaggatact ggtcacgaca tttacttgcc cataggtcta tggcatcaag atccccttct
    10801 agagctctga ccagtgccca taactcatcc aaggtgtggc gaattaggat atatctatcc
    10861 ttactttcac atgaacacag atccttagcg tggataagac atgcaaaccg gttgggggaa
    10921 cacttgcagc ttgaagcaga catatgcaaa tcatagaagc acaagaagca ctcccgttct
    10981 ctcttattgt cgaaatcacc ctccatcttt cgcaagctaa aaccatcttg aaggtgattt
    11041 agtctttctt cttccatctg cacacgcttc tgctaaccac agctatgtta gtgccattga
    11101 agatgtttgt ttcaatctta tcaaaaatca ggtagactag tacactatta cttcagagga
    11161 tagttgtcaa tctatgaaat gtaacatata gcctatgctt ttttgagtgt gtattgtaca
    11221 tgcatgcaca cagaaaaaag aacagagaac caaaattacc ttgactgcct tcgtaagcaa
    11281 tccatcctca ctacaaaccc ttttccatct cgcaatcaca ggagtcttct tctttgaaag
    11341 tgaaagctcc cagaggcaat aagtagcttc catagcagct ccaagaagca gcttgtcatg
    11401 tgacaatgaa ctctttcgcc gctgcttgct atagccttcc actgcattct gtccatgaac
    11461 cagccaatca actggtgcaa catttactgc ttccgcacag ttgaaaccac aattaaaccc
    11521 agaatgatag gccttaggga aggttagaat gaattctcca ctgcgctgga cagctcgata
    11581 gacaggtact ccctcctctt ttaagattct tggagataac tgagtgacct gataacaata
    11641 ggaggtgcag agtgagatac atattatctt aaccaaaaga cagctctcgc catatcatct
    11701 ccaaaattac acacgtaagg agttctaatt aatcagcaga gcttgaaatg aaaaacgaat
    11761 tgggagaaac tttcaccagt tgatgtagca agtcaggctg ttcttcaaac aaatctggaa
    11821 gacgcttttt cattacattt tcaaaagact cagcatggtt tccagggatc ccataccaaa
    11881 cttttggatc acctgtgtgt aagtagttca tggaataaag gtgatgatct tcaacatgct
    11941 gtttcaaaca aagtaagggg gtaaatagca gatggttagt acatagctcc tagaaagcac
    12001 acaaaaaatg catcaattcc ataactttgg tcccaaaggc atgacatcct ttaagtaata
    12061 aaattttgat tcaataacag gttttaatag aactccatca gctttttttc agcaacatat
    12121 aatttccagg aaatatcaaa agattattca tgcgattcag taattagcaa gagtctaccc
    12181 taagaaaatg aaacttaccc aacaaaaagt tgaaaagcac attccaacat aaagccatgg
    12241 cacaatgact cctgagatat cgcagctctc aaaagctaga actgatccgg gcagacggga
    12301 caaattattt aggttccaac cacattgaga gtattgatct gcttcactta taggataccc
    12361 cggtttatac tttgggaaac cacttccaaa tttctttgtt tccaagtcag ctccatagta
    12421 cacctgtcat aggaccaaaa acaagagtag caagttcgaa gaacatcaaa ttcaaataaa
    12481 tgcaaaagaa aatggtgaaa ccaagaaaga cacaaatagt tgatatgtca ttatattgct
    12541 gcatgtacat acctctactt catcagttgc ctgctccact atccgccaat attcaccttc
    12601 aaggtcctta accttaggtt taaatttctt attttcagac gctttggaac caggatgatc
    12661 ctctgactga aaatagcatt ccttaaagta ttcatcatac ttctgaaatt cttccagggt
    12721 aaaatctggc ccagtctgaa aaccaaattt tccttcacta tcactagagc cagaggaagc
    12781 cgtatcacag cctgaatctc tttttcttct tgtgtatcca attttagaga ttcttctccg
    12841 ttttctcttc ttggttttag tagatttttt aatcggttct cggttttgaa gcaagtcaat
    12901 gaactgaatc cgggtgggaa atttagaatt ctcccatatt tttttctcct tcagagggca
    12961 agggggcctc catgcaacag gcggcacaat tcgacatatg ccatatgatt ctgctttaga
    13021 gcgcaacttc tctatgtaac caagtggatc atcaaaatcc tgaccatgaa gcagtatcat
    13081 aaggatcaaa attttcagat aagataacaa tttaactacc aagacagaac caaaattgca
    13141 actcgagtat tttaaatacc tcattagtgg gataaaagat gggagcgtca tcaaccaaag
    13201 gcctgcaagc ttctgatgga ttccacctag cagttatctt caaatacgca acaaaacgat
    13261 atcattcatg aatcagattc atctgctctt ggacaatcag atagctaaca caacaaacaa
    13321 tctaataaga tccagatgag attgctaaat tcttaagacc caagtaaata gattttgctt
    13381 aatctaaaac aaatacaata agaacgactc tcagaaagat gagatctttg aaggaagata
    13441 ctgataagca aaagccacga acttaattaa aactagaatt gattggattt tcagactcaa
    13501 acgggtttag cacatagata agccatcaac ctctttaccc aaccaatact ttgctagaaa
    13561 cccaattaac ttactttagg actagaagga gtagagtcag gttcaagact actctctcct
    13621 ttaatcgatt gtttctcaga atcttcctgc aagaaataaa cacaattcca cattcagaaa
    13681 ctgcaacagt gctaattgaa tctagcaaac atgcaaatta gaggttacaa atggaggaga
    13741 aagcaaaaaa cacaaatcaa agaagcaaaa ggataaagat gagaaacctc catagccaca
    13801 gactctgcta gagatgcaag ctgatccatt tacagtgaga ttaagttcac aaagatcgat
    13861 acttttaacc cgtgacctaa ggaagaacaa agatctgtta aataaaaaac aaacccttac
    13921 ttgatgaaat gcagtgccgt tttcgaaacc ctaaaaaaac ccagaacgca tatagatttg
    13981 tgtctggaaa tagagagtga gatgataagt gtaccttcgt gaaggggcca agattcggaa
    14041 caaaagcttt tcccaagatt gaatttgact ctcgaatttt atttgtaacg tatagtataa
    14101 atctaaaacc ttttggttaa atatctgtga aagaggtttt tttttttctt tccttctttg
    14161 atgagttaag aaaaaaaaaa cttagagaag aatttattag gttaacgatt aaagcaagta
    14221 gatttcttgg agtccgatct aagaaaaggg tttagaacat aaaaagaaga agccgccaaa
    14281 tgtgattttg ttttgtaata aaatcttcaa tccatttata ccaaataaaa ttcagtagtt
    14341 ttataaacag aaaattaaaa aaaaaaaaaa ctgaattaat cttcaaatta agtagttttg
    14401 ttgatttgta tatctgaact aaattaaaca gttatactat ttacacttca tttttactaa
    14461 ccaaaaagaa aaggatttca atagttatga ttattgattc acatttatta gagacagtag
    14521 gatcattatt tacaacacaa aaattaagtt gtatgggaac tacaatgcga tagaactaat
    14581 actgatttgt tttttagtcg gttgatataa ttcattttaa tgtaaaaaac taacaattga
    14641 ttagaattat atttccataa atagaagaag tagttgagaa ataaaaggta taaattatct
    14701 tgaattggtc ttatctaatc atctattgta gatttttctc attgtttcaa tgtactatat
    14761 actttgtgaa tttttttttg tcattttaga attatataaa aagataagag agaaatgaaa
    14821 acttgaatac aaatgtgaaa gtgatagaac aaaggagtgc attacacaat ttgctagtga
    14881 cttgagagac aaacacacaa agtaagccac aacatctata aaatggtcac acacatgttg
    14941 ctgaattttt ggccacgatg ttttagacga cggaggttgt ggtggcggtg gtggagaagt
    15001 ttttagaatt gattaagagg cgggaaatag agacggcgga cacgaggagc attagaatga
    15061 ctccggcgaa tgctgcgatg attgctcctg cgccgtgatc acagtaagtg gtgaacctgt
    15121 cgcagacttt gttccacttg gcgtgcttgt ttccgttctt ccctagctct gccacgaaca
    15181 ccgccgcgtt tgcagccgca gatactagtg ttgcgtttag ctttttttcc aacgtttttt
    15241 tagattatca agaaaccgtt tagctttttt tccaaacgtt caaactaatt caattgaaat
    15301 caaaccagta aattgatttg aaaaccaatg tttataccat gtcaagaatg gcgatagaga
    15361 ggagacgaag acctttgtac tccagtttcc ggctgaaaat ctgaaccaca atcatcaaca
    15421 agttgtggaa gctcaccatt acattagcta taacaaagaa cctgtaaaac caagaatcaa
    15481 tgttaataac tcaaaaacat tttgctttca atatgtatcg aatcgatttc gtatagggtt
    15541 ttactcgctt acacaaaagc cggtgtgtgc tgaaacttag cggttaaagt ggctttaata
    15601 ggaacagtac caatggtggc cacaaccaag gtctttgtct ctttgtttag tgacattaca
    15661 atggctgcag ctaaagtagc catgaatgca aatattctca gccccaagag tagtttccag
    15721 ctctttgtgg taccaccagc caccacaatc ttctctctgg ccatggtggg tgaagggggc
    15781 ggccgcggag cctgcttttt tgtacaaact tgttgatgat cccgggcccg cggtaccgtc
    15841 gactgcagaa ttcaattctg agatttttca aatcagtgcg caagacgtga cgtaagtatc
    15901 cgagtcagtt tttatttttc tactaatttg gtcgtttatt tcggcgtgta ggacatggca
    15961 accgggcctg aatttcgcgg gtattctgtt tctattccaa ctttttcttg atccgcagcc
    16021 attaacgact tttgaataga tacgctgaca cgccaagcct cgctagtcaa aagtgtacca
    16081 aacaacgctt tacagcaaga acggaatgcg cgtgacgctc gcggtgacgc catttcgcct
    16141 tttcagaaat ggataaatag ccttgcttcc tattatatct tcccaaatta ccaatacatt
    16201 acactagcat ctgaatttca taaccaatct cgatacacca aatcgaatcg atgagcccag
    16261 aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga catgccggcg gtctgcacca
    16321 tcgtcaacca ctacatcgag acaagcacgg tcaacttccg taccgagccg caggaaccgc
    16381 aggagtggac ggacgacctc gtccgtctgc gggagcgcta tccctggctc gtcgccgagg
    16441 tggacggcga ggtcgccggc atcgcctacg cgggcccctg gaaggcacgc aacgcctacg
    16501 actggacggc cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg ggactgggct
    16561 ccacgctcta cacccacctg ctgaagtccc tggaggcaca gggcttcaag agcgtggtcg
    16621 ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc ggatatgccc
    16681 cccgcggcat gctgcgggcg gccggcttca agcacgggaa ctggcatgac gtgggtttct
    16741 ggcagctgga cttcagcctg ccggtaccgc cccgtccggt cctgcccgtc accgagattt
    16801 gactgcagaa tcttggactc ccatgttggc aaaggcaacc aaacaaacaa tgaatgatcc
    16861 gctcctgcat atggggcggt ttgagtattt caactgccat ttgggctgaa ttgaagacat
    16921 gctcctgtca gaaattccgt gatcttactc aatattcagt aatctcggcc aatatcctaa
    16981 atgtgcgtgg ctttatctgt ctttgtattg tttcatcaat tcatgtaacg tttgcttttc
    17041 ttatgaattt tcaaataaat tatcagatcc gagctcgaat taattcggcg ttaattcagt
    17101 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
    17161 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
    17221 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
    17281 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
    17341 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
    17401 tgcctgtgat caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt
    17461 tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag
    17521 tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa
    17581 agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
    17641 gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
    17701 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
    17761 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
    17821 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
    17881 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
    17941 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
    18001 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
    18061 gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt
    18121 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
    18181 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
    18241 gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa
    18301 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
    18361 aaatattata ttttactgga tgaattgttt tagtacctag aatgcatgac caaaatccct
    18421 taacgctgag agatcccctc ataatttccc caaagcgtaa ccatgtgtga ataaattttg
    18481 agctagtagg gttgcagcca cgagtaagtc ttcccttgtt attgtgtagc cagaatgccg
    18541 caaaacttcc atgcctaagc gaactgttga gagtacgttt cgatttctga ctgtgttagc
    18601 ctggaagtgc ttgtcccaac cttgtttctg agcatgaacg cccgcaagcc aacatgttag
    18661 ttgaagcatc agggcgatta gcagcatgat atcaaaacgc tctgagctgc tcgttcggct
    18721 atggcgtagg cctagtccgt aggcaggact tttcaagtct cggaaggttt cttcaatctg
    18781 cattcgcttc gaatagatat taacaagttg tttgggtgtt cgaatttcaa caggtaagtt
    18841 agttgctaga atccatggct cctttgccga cgctgagtag attttaggtg acgggtggtg
    18901 acaatgagtc cgtgtcgagc gctgattttt tcggccttta gagcgagatt tatacaatag
    18961 aatttggcat gagattggat tgcttttagt cagcctctta tagcctaaag tctttgagtg
    19021 actagatgac atatcatgta agttgctgat aggtttccag ttttccgctc ctaggtctgc
    19081 atattgtact tttcctctta ctcgacttaa ccagtaccaa cccagcttct caacggattt
    19141 ataccatggc actttaaagc cagcatcact gacaatgagc ggtgtggtgt tactcggtag
    19201 aatgctcgca aggtcggcta gaaattggtc atgagctttc tttgaacatt gctctgaaag
    19261 cgggaacgct ttctcataaa gagtaacaga acgaccgtgt agtgcgactg aagctcgcaa
    19321 taccataagc cgtttttgct cacggatatc agaccagtca acaagtacaa tgggcatcgt
    19381 attgcccgaa cagataaagc tagcatgcca acggtataca gcgagtcgct ctttgtggag
    19441 gtgacgatta cctaacaatc ggtcgattcg tttgatgtta tgttttgttc tcgctttggt
    19501 tggcaggtta cggccaagtt cggtaagagt gagagtttta cagtcaagta aggcgtggca
    19561 agccaacgtt aagctgttga gtcgttttaa gtgtaattcg gggcagaatt ggtaaagaga
    19621 gtcgtgtaaa atatcgagtt cgcacatttt gttgtctgat tattgatttt tggcgaaacc
    19681 atttgatcat atgacaagat gtgtatctac cttaacttaa tgattttgat aaaaatcatt
    19741 aggggattca tcagccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    19801 aagatcaaag gatcttc
//
