LOCUS       sequence-401427-       15806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express MORC1-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15806)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Li Z, Feng S, Kuo HY, Kan RL, 
            Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, 
            Wohlschlegel J, Jacobsen SE
  TITLE     A gene silencing screen uncovers diverse tools for targeted gene 
            repression in Arabidopsis.
  JOURNAL   Nat Plants. 2023 Mar 6. doi: 10.1038/s41477-023-01362-8.
  PUBMED    36879017
REFERENCE   2  (bases 1 to 15806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2023 Mar 6. doi: 10.1038/s41477-023-01362-8."
COMMENT     Sequence Label: pMDC123-MORC1-ZF
FEATURES             Location/Qualifiers
     source          1..15806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1077..1868)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2276..2470
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2536..3609)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4038..4667)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    5968..5992
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6180..6202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6194..6211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6195..6211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8309..8353
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8381..8404
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8915..8986
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    9026..9050
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11029..11053)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11865..11881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11865..11881)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11878..11900)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11889..11905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11913..11919,11920..11937,11938..11943))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11958..11979
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12170..12846
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12783..12803
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             12891..13442
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    13449..13623
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13701..13725
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     mobile_element  14136..14903
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             14926..15720
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccttat
     1081 ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct
     1141 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     1201 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     1261 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     1321 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     1381 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     1441 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     1501 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     1561 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     1621 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     1681 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     1741 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     1801 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     1861 tccctcataa tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     1921 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     1981 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     2041 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatggc gccggcggtc
     2101 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     2161 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     2221 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     2281 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     2341 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     2401 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     2461 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     2521 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     2581 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     2641 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     2701 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     2761 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     2821 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     2881 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     2941 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     3001 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     3061 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     3121 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     3181 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     3241 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     3301 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     3361 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
     3421 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     3481 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     3541 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
     3601 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcaggca
     3661 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
     3721 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
     3781 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
     3841 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
     3901 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
     3961 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
     4021 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
     4081 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
     4141 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
     4201 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
     4261 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
     4321 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
     4381 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
     4441 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
     4501 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
     4561 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
     4621 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
     4681 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
     4741 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
     4801 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
     4861 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
     4921 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
     4981 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
     5041 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
     5101 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
     5161 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
     5221 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
     5281 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
     5341 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
     5401 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
     5461 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
     5521 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
     5581 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
     5641 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
     5701 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
     5761 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
     5821 actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac cctgtggttg
     5881 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt
     5941 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
     6001 tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagcattc
     6061 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     6121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     6181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtcgactc
     6241 tagaggatcc ccgggtaccg ggccccccct cgaggcgcgc cacgcgtagt ctagctcaac
     6301 agagctttta acccaaattg gtacaataga atacaacttt agatcataat tctcaaaaga
     6361 aagagattcc ttagctattc tatctgccac tccatttcct tctcggcttg tatgcacaag
     6421 cataaaatcc tcaaacttgc taagtagata ctttatgtct tggataattg gattgagact
     6481 tgacaagcat aactttcatg taaccaaaga cacaagttgc tgagaatcca cctcaaaaat
     6541 gatcttccta taattgaatc gggataatga cagcacagcc catctaagag cctccacttc
     6601 tacttccagc acgcttctta cttttaccac agctcttgca cctaaccata acaccttccc
     6661 tgtatgatcg cgaagcaccc accctaagcc acattttaat ccttctgttg gccatgcccc
     6721 atcaaagttg cacttaaccc aagattgtgg tggagcttcc catgtttctc gtctgtcccg
     6781 acggtgttgt ggttggtgct ttccttacat tctgagcctc tttccttcta atccactcat
     6841 ctgcatcttc ttgtgtcctt actaatacct cattggttcc aaattccctc cctttaagca
     6901 ccagctcgtt tctgttcttc cacagcctcc caagtatcca agggactaaa gcctccacat
     6961 tcttcagatc aggatattct tgtttaagat gttgaactct atggaggttt gtatgaactg
     7021 atgatctagg accggataag ttcccttctt catagcgaac ttattcaaag aatgttttgt
     7081 gtatcattct tgttacattg ttattaatga aaaaatatta ttggtcattg gactgaacac
     7141 gagtgttaaa tatggaccag gccccaaata agatccattg atatatgaat taaataacaa
     7201 gaataaatcg agtcaccaaa ccacttgcct tttttaacga gacttgttca ccaacttgat
     7261 acaaaagtca ttatcctatg caaatcaata atcatacaaa aatatccaat aacactaaaa
     7321 aattaaaaga aatggataat ttcacaatat gttatacgat aaagaagtta cttttccaag
     7381 aaattcactg attttataag cccacttgca ttagataaat ggcaaaaaaa aacaaaaagg
     7441 aaaagaaata aagcacgaag aattctagaa aatacgaaat acgcttcaat gcagtgggac
     7501 ccacggttca attattgcca attttcagct ccaccgtata tttaaaaaat aaaacgataa
     7561 tgctaaaaaa atataaatcg taacgatcgt taaatctcaa cggctggatc ttatgacgac
     7621 cgttagaaat tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg gttgcagccg
     7681 gcacacacga gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa cctaaaaata
     7741 aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt gtcattttat
     7801 tattagctat tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc gtcttttctt
     7861 cttcttcttc tataaaacaa tacccaaaga gctcttcttc ttcacaattc agatttcaat
     7921 ttctcaaaat cttaaaaact ttctctcaat tctctctacc gtgatcaagg taaatttctg
     7981 tgttccttat tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat ttcgtatatg
     8041 ttctttggtt tagattctgt taatcttaga tcgaagacga ttttctgggt ttgatcgtta
     8101 gatatcatct taattctcga ttagggtttc atagatatca tccgatttgt tcaaataatt
     8161 tgagttttgt cgaataatta ctcttcgatt tgtgatttct atctagatct ggtgttagtt
     8221 tctagtttgt gcgatcgaat ttgtcgatta atctgagttt ttctgattaa cagacgcgtc
     8281 catggttggg taccaacaaa tggctggtgg acttaacgat atcttcgaag ctcagaagat
     8341 tgaatggcat gaggatactg gtggatctag catccccgga cttgaggttc ttttccaagg
     8401 acctctcgag ccttataaat gccctgagtg tggaaaatct ttctctcaat ctggagatct
     8461 taggaggcac caaaggaccc ataccggtga aaaaccttat aaatgccctg agtgtggaaa
     8521 atcattcagt aggagtgatc atcttaccac ccatcagagg acccatactg gtgaaaaacc
     8581 ttataaatgc cctgagtgtg gaaaatcatt ctcacaatct tcatctcttg ttaggcatca
     8641 gaggactcat accggagaaa aaccttataa atgccctgag tgtggaaaat cattctcaac
     8701 atctggaaac cttgttaggc accaaaggac ccatactggt gaaaaacctt ataaatgccc
     8761 tgagtgtgga aaatcattct ctcagagagc aaaccttagg gctcatcaaa ggacacacac
     8821 tggagaaaaa ccttataaat gccctgagtg tggaaaatct ttctctagga gtgataagct
     8881 caccgagcat caaaggactc acaccggact cgaggactac aaagacgatg acgacaaaga
     8941 ctacaaagac gatgacgaca aagactacaa agacgatgac gacaaaaagc ttgatatcgc
     9001 catggggggc gcgccaagct atcaaacaag tttgtacaaa aaagcagaag ggccgtcaag
     9061 gccagaagga gatataacca tggcgaaaaa ttacacagtc gccgatgtcg taaatatcga
     9121 cagtgacagt gacagtgacg acgacaacgg aggagtaata ggaatggttc cgagcttggc
     9181 atctctaata gagaatcaga aagtttcgat cgctgatgct gcgacggtgg ctcctcgaga
     9241 aaccctagag tgccgaagtt tctggaaagc cggcgaaaac tttgtgatcc cttcaagtgt
     9301 tactctaact gccataggta tggttgagca tgctcgtgtt catccaaagt ttcttcactc
     9361 gaatgctact tcacataaat gggcttttgg agcaatcgcc gagctacttg acaatgcggt
     9421 tgatgagata caaaacggtg ctaccgttgt caagattgat aagatcaata ttgttaaaga
     9481 taatacacct gccttagttt tccaagataa tggtggtggg atggatccta atgggatcag
     9541 aaaatgcatg agcttaggct actcgtcaaa gaagtctaat acgacaattg gacaatatgg
     9601 aaatggtttc aagacaagta caatgagact tggagctgat gccatggttt ttagtcgctc
     9661 aactcgtgga ggcaaatcta cacagagcat tggtcttctg tcctatacat tccttaggaa
     9721 aactggtcag gatgatgtga tcgttcctat gattgatttc gatatatcca gtgatagccc
     9781 ccaaccaatt atttatgggt ctcccggaga ttggtctacc aaccttaaca ttcttctcaa
     9841 atggtccccg ttttcaacaa tggtcgaact tttgcagcag ttcgaggata ttggaacaca
     9901 tggaacaaaa gtaattatat acaacttgtg gcttaatgat gaaggaatct atgagctgag
     9961 ttttgacgac gatgatgtgg atatacggct ccgagatgaa aatgcccagg acgggaaacg
    10021 gttgcacgct aaaacattgg aagtaagatc tcatatttcg taccgctatc gacattctct
    10081 aagggcttac atttccatgt tatatctcaa aaagttcaaa aacttcaaaa ttattcttcg
    10141 gggagtctct gtggcgcaat tcaacattgc tgatgaattc agacatcctg agacaataat
    10201 gtacaaaccc caagcagctg cagtggatta tgctgccact ggaattaaag ttggattcat
    10261 caaggaggcc cctaaacttc caatttgtgg tttcaatgtc tatcacaaaa accgtctcat
    10321 ccggccattc tggaaggtcg ttctggaagg gtcaacgaga ggcaatggtg ttatgggagt
    10381 tcttgaggca aacttcatag aaccagccca cgacaagcaa gattttgaga ggtcttctct
    10441 atttctgcgg ctggaggcga ggctgaaaag gataacctct gattactggc aaaatcactg
    10501 tcacattttc ggataccaaa ctgctcaaat ccctgcagat aagtcgaaaa ggacagtaat
    10561 acctgatcaa ccgcctactg tcaatacata caatccttcg cctttgcctt ctgatagaat
    10621 cagtcatggt ggtcctataa tacgtgaaat caatcttagt aacgccactt caagccgaac
    10681 agctgctgtt gccgcgccac atttgagaaa ttacacaggt ttaagaaata acttccaacc
    10741 cgtgcagctc aacccacaac cacctgcagc tggcgatact ggaaacaacc tcgttggcaa
    10801 gttagcggct gagatacgcg aagagaactt acaactcttc atgaggtgcg aggaatatgt
    10861 aaagaaagag aatgaagtag aacagacggt aaagagctta gaaaaagaac tggaagaaat
    10921 taaaagtaaa tgtgcacaac ttgctctgct tgtggatgct aagaagaagg agatgcaaca
    10981 agtttagtaa aagcgttacg agttaaaact ctctggaagg cctcatgggc ccagctttct
    11041 tgtacaaagt ggttcgataa ttcttaatta actagttcta gagcggccgc caccgcggtg
    11101 gagctcctag gtgagtctag agagttaatt aagacccggg actagtccct agagtcctgc
    11161 tttaatgaga tatgcgagac gcctatgatc gcatgatatt tgctttcaat tctgttgtgc
    11221 acgttgtaaa aaacctgagc atgtgtagct cagatcctta ccgccggttt cggttcattc
    11281 taatgaatat atcacccgtt actatcgtat ttttatgaat aatattctcc gttcaattta
    11341 ctgattgtac cctactactt atatgtacaa tattaaaatg aaaacaatat attgtgctga
    11401 ataggtttat agcgacatct atgatagagc gccacaataa caaacaattg cgttttatta
    11461 ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa acctaaaaga ctgattacat
    11521 aaatcttatt caaatttcaa aagtgcccca ggggctagta tctacgacac accgagcggc
    11581 gaactaataa cgctcactga agggaactcc ggttccccgc cggcgcgcat gggtgagatt
    11641 ccttgaagtt gagtattggc cgtccgctct accgaaagtt acgggcacca ttcaacccgg
    11701 tccagcacgg cggccgggta accgacttgc tgccccgaga attatgcagc atttttttgg
    11761 tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac agtgacgaca aatcgttggg
    11821 cgggtccagg gcgaattttg cgacgagctc gaattcgtaa tcatgtcata gctgtttcct
    11881 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    11941 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    12001 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    12061 agaggcggtt tgcgtattgg ctagagcagc ttgccaacat ggtggagcac gacactctcg
    12121 tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc
    12181 aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca
    12241 tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa
    12301 aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
    12361 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
    12421 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
    12481 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
    12541 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
    12601 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
    12661 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
    12721 tcttcaaagc aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc
    12781 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca
    12841 cgctgaaatc accagtctct ctctacaaat ctatctctct cgagtctacc atgagcccag
    12901 aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga catgccggcg gtctgcacca
    12961 tcgtcaacca ctacatcgag acaagcacgg tcaacttccg taccgagccg caggaaccgc
    13021 aggagtggac ggacgacctc gtccgtctgc gggagcgcta tccctggctc gtcgccgagg
    13081 tggacggcga ggtcgccggc atcgcctacg cgggcccctg gaaggcacgc aacgcctacg
    13141 actggacggc cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg ggactgggct
    13201 ccacgctcta cacccacctg ctgaagtccc tggaggcaca gggcttcaag agcgtggtcg
    13261 ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc ggatatgccc
    13321 cccgcggcat gctgcgggcg gccggcttca agcacgggaa ctggcatgac gtgggtttct
    13381 ggcagctgga cttcagcctg ccggtaccgc cccgtccggt cctgcccgtc accgagattt
    13441 gactcgagtt tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct
    13501 atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta
    13561 aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag
    13621 atcccccgaa ttaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta
    13681 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
    13741 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg
    13801 gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta
    13861 ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag
    13921 catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg
    13981 gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct
    14041 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
    14101 cttcttgggg tatctttaaa tactgtagaa aagagggtaa tgactccaac ttattgatag
    14161 tgttttatgt tcagataatg cccgatgact ttgtcatgca gctccaccga ttttgagaac
    14221 gacagcgact tccgtcccag ccgtgccagg tgctgcctca gattcaggtt atgccgctca
    14281 attcgctgcg tatatcgctt gctgattacg tgcagctttc ccttcaggcg ggattcatac
    14341 agcggccagc catccgtcat ccatatcacc acgtcaaagg gtgacagcag gctcataaga
    14401 cgccccagcg tcgccatagt gcgttcaccg aatacgtgcg caacaaccgt cttccggaga
    14461 ctgtcatacg cgtaaaacag ccagcgctgg cgcgatttag ccccgacata gccccactgt
    14521 tcgtccattt ccgcgcagac gatgacgtca ctgcccggct gtatgcgcga ggttaccgac
    14581 tgcggcctga gttttttaag tgacgtaaaa tcgtgttgag gccaacgccc ataatgcggg
    14641 ctgttgcccg gcatccaacg ccattcatgg ccatatcaat gattttctgg tgcgtaccgg
    14701 gttgagaagc ggtgtaagtg aactgcagtt gccatgtttt acggcagtga gagcagagat
    14761 agcgctgatg tccggcggtg cttttgccgt tacgcaccac cccgtcagta gctgaacagg
    14821 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
    14881 atcagtaagt tggcagcatc accgaaaaga ggaaggaaat aataaatggc taaaatgaga
    14941 atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga
    15001 atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg
    15061 acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta
    15121 tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg
    15181 agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa
    15241 caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc
    15301 gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac
    15361 ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt
    15421 aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc
    15481 ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc
    15541 tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc
    15601 cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg
    15661 gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga attgttttag
    15721 tacctagaat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    15781 cccgtagaaa agatcaaagg atcttc
//
