LOCUS       sequence-401486-       14354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express DMS3-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14354)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Li Z, Feng S, Kuo HY, Kan RL, 
            Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, 
            Wohlschlegel J, Jacobsen SE
  TITLE     A gene silencing screen uncovers diverse tools for targeted gene 
            repression in Arabidopsis.
  JOURNAL   Nat Plants. 2023 Mar 6. doi: 10.1038/s41477-023-01362-8.
  PUBMED    36879017
REFERENCE   2  (bases 1 to 14354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2023 Mar 6. doi: 10.1038/s41477-023-01362-8."
COMMENT     Sequence Label: pMDC123-DMS3-ZF
FEATURES             Location/Qualifiers
     source          1..14354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1077..1868)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2276..2470
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2536..3609)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4038..4667)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    5968..5992
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6180..6202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6194..6211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6195..6211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8309..8353
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8381..8404
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8915..8986
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    9026..9050
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10353..10377)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11189..11205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11189..11205)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11202..11224)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11213..11229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11237..11243,11244..11261,11262..11267))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11282..11303
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11494..12170
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12107..12127
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             12215..12766
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    12773..12947
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13025..13049
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             13474..14268
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccttat
     1081 ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct
     1141 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     1201 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     1261 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     1321 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     1381 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     1441 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     1501 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     1561 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     1621 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     1681 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     1741 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     1801 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     1861 tccctcataa tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     1921 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     1981 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     2041 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatggc gccggcggtc
     2101 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     2161 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     2221 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     2281 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     2341 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     2401 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     2461 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     2521 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     2581 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     2641 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     2701 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     2761 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     2821 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     2881 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     2941 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     3001 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     3061 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     3121 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     3181 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     3241 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     3301 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     3361 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
     3421 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     3481 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     3541 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
     3601 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcaggca
     3661 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
     3721 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
     3781 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
     3841 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
     3901 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
     3961 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
     4021 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
     4081 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
     4141 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
     4201 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
     4261 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
     4321 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
     4381 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
     4441 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
     4501 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
     4561 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
     4621 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
     4681 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
     4741 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
     4801 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
     4861 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
     4921 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
     4981 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
     5041 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
     5101 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
     5161 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
     5221 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
     5281 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
     5341 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
     5401 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
     5461 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
     5521 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
     5581 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
     5641 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
     5701 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
     5761 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
     5821 actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac cctgtggttg
     5881 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt
     5941 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
     6001 tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagcattc
     6061 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     6121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     6181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtcgactc
     6241 tagaggatcc ccgggtaccg ggccccccct cgaggcgcgc cacgcgtagt ctagctcaac
     6301 agagctttta acccaaattg gtacaataga atacaacttt agatcataat tctcaaaaga
     6361 aagagattcc ttagctattc tatctgccac tccatttcct tctcggcttg tatgcacaag
     6421 cataaaatcc tcaaacttgc taagtagata ctttatgtct tggataattg gattgagact
     6481 tgacaagcat aactttcatg taaccaaaga cacaagttgc tgagaatcca cctcaaaaat
     6541 gatcttccta taattgaatc gggataatga cagcacagcc catctaagag cctccacttc
     6601 tacttccagc acgcttctta cttttaccac agctcttgca cctaaccata acaccttccc
     6661 tgtatgatcg cgaagcaccc accctaagcc acattttaat ccttctgttg gccatgcccc
     6721 atcaaagttg cacttaaccc aagattgtgg tggagcttcc catgtttctc gtctgtcccg
     6781 acggtgttgt ggttggtgct ttccttacat tctgagcctc tttccttcta atccactcat
     6841 ctgcatcttc ttgtgtcctt actaatacct cattggttcc aaattccctc cctttaagca
     6901 ccagctcgtt tctgttcttc cacagcctcc caagtatcca agggactaaa gcctccacat
     6961 tcttcagatc aggatattct tgtttaagat gttgaactct atggaggttt gtatgaactg
     7021 atgatctagg accggataag ttcccttctt catagcgaac ttattcaaag aatgttttgt
     7081 gtatcattct tgttacattg ttattaatga aaaaatatta ttggtcattg gactgaacac
     7141 gagtgttaaa tatggaccag gccccaaata agatccattg atatatgaat taaataacaa
     7201 gaataaatcg agtcaccaaa ccacttgcct tttttaacga gacttgttca ccaacttgat
     7261 acaaaagtca ttatcctatg caaatcaata atcatacaaa aatatccaat aacactaaaa
     7321 aattaaaaga aatggataat ttcacaatat gttatacgat aaagaagtta cttttccaag
     7381 aaattcactg attttataag cccacttgca ttagataaat ggcaaaaaaa aacaaaaagg
     7441 aaaagaaata aagcacgaag aattctagaa aatacgaaat acgcttcaat gcagtgggac
     7501 ccacggttca attattgcca attttcagct ccaccgtata tttaaaaaat aaaacgataa
     7561 tgctaaaaaa atataaatcg taacgatcgt taaatctcaa cggctggatc ttatgacgac
     7621 cgttagaaat tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg gttgcagccg
     7681 gcacacacga gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa cctaaaaata
     7741 aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt gtcattttat
     7801 tattagctat tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc gtcttttctt
     7861 cttcttcttc tataaaacaa tacccaaaga gctcttcttc ttcacaattc agatttcaat
     7921 ttctcaaaat cttaaaaact ttctctcaat tctctctacc gtgatcaagg taaatttctg
     7981 tgttccttat tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat ttcgtatatg
     8041 ttctttggtt tagattctgt taatcttaga tcgaagacga ttttctgggt ttgatcgtta
     8101 gatatcatct taattctcga ttagggtttc atagatatca tccgatttgt tcaaataatt
     8161 tgagttttgt cgaataatta ctcttcgatt tgtgatttct atctagatct ggtgttagtt
     8221 tctagtttgt gcgatcgaat ttgtcgatta atctgagttt ttctgattaa cagacgcgtc
     8281 catggttggg taccaacaaa tggctggtgg acttaacgat atcttcgaag ctcagaagat
     8341 tgaatggcat gaggatactg gtggatctag catccccgga cttgaggttc ttttccaagg
     8401 acctctcgag ccttataaat gccctgagtg tggaaaatct ttctctcaat ctggagatct
     8461 taggaggcac caaaggaccc ataccggtga aaaaccttat aaatgccctg agtgtggaaa
     8521 atcattcagt aggagtgatc atcttaccac ccatcagagg acccatactg gtgaaaaacc
     8581 ttataaatgc cctgagtgtg gaaaatcatt ctcacaatct tcatctcttg ttaggcatca
     8641 gaggactcat accggagaaa aaccttataa atgccctgag tgtggaaaat cattctcaac
     8701 atctggaaac cttgttaggc accaaaggac ccatactggt gaaaaacctt ataaatgccc
     8761 tgagtgtgga aaatcattct ctcagagagc aaaccttagg gctcatcaaa ggacacacac
     8821 tggagaaaaa ccttataaat gccctgagtg tggaaaatct ttctctagga gtgataagct
     8881 caccgagcat caaaggactc acaccggact cgaggactac aaagacgatg acgacaaaga
     8941 ctacaaagac gatgacgaca aagactacaa agacgatgac gacaaaaagc ttgatatcgc
     9001 catggggggc gcgccaagct atcaaacaag tttgtacaaa aaagcagaag ggccgtcaag
     9061 gccagaagga gatataacca tgtatccgac tggtcaacag atttcgtttc agaccactcc
     9121 gttgaatgtt caagatccaa caaggatgat gaatttggat caatcttcac cagttgcaag
     9181 aaatgaaact cagaatggag gaggcattgc tcacgctgag tttgctatgt tcaattccaa
     9241 gaggcttgaa tctgatcttg aagctatggg taacaaaatc aaacagcatg aggataatct
     9301 caagtttctc aagtctcaga aaaacaaaat ggatgaagct atcgtcgact tgcaagttca
     9361 tatgagtaag cttaactcgt ctcctactcc tagaagtgaa aactctgata acagtcttca
     9421 gggtgaagat atcaatgcac agatccttcg ccacgaaaac tcagctgctg gagttttaag
     9481 tctggttgag actcttcatg gtgctcaagc ttctcagttg atgttgacaa aaggtgttgt
     9541 tggtgttgta gcgaaacttg ggaaggtcaa tgatgaaaat ctcagccaga ttttgtcgaa
     9601 ttatttgggg actcgctcaa tgttggcagt tgtatgcagg aattatgaaa gtgttacggc
     9661 tttagaggct tatgataacc atggcaacat tgatataaat gctggccttc attgccttgg
     9721 ctcttcgatt ggaagagaga ttggagacag ttttgatgcc atctgccttg aaaacctgag
     9781 gccgtatgtt ggtcagcaca tagctgatga tctgcaaaga aggcttgatc ttctgaaacc
     9841 aaaattacct aatggtgagt gtcctcccgg gtttctcgga tttgcagtga atatgataca
     9901 gatcgatcca gcttacttgc tctgtgtcac atcatatgga tatggtcttc gtgagacctt
     9961 gttctacaat ctattctctc gccttcaagt ttacaaaaca agggccgata tgattagtgc
    10021 cctcccatgc ataagtgatg gtgcggtatc tttggatgga ggaatcatca ggaaaacggg
    10081 gatcttcaat cttggaaacc gtgatgaggt gaacgtgaga tttgcaaagc caactgcttc
    10141 tcgcacgatg gacaactata gtgaggcgga gaagaaaatg aaagagctga aatggaaaaa
    10201 ggagaaaacg ctggaggaca ttaagcggga gcaagtgctc cgtgagcatg ccgtcttcaa
    10261 ctttggcaag aagaaagaag aatttgttcg atgcttggct cagagttcat gcactaatca
    10321 gccaatgaac acacccagat gaggcctcat gggcccagct ttcttgtaca aagtggttcg
    10381 ataattctta attaactagt tctagagcgg ccgccaccgc ggtggagctc ctaggtgagt
    10441 ctagagagtt aattaagacc cgggactagt ccctagagtc ctgctttaat gagatatgcg
    10501 agacgcctat gatcgcatga tatttgcttt caattctgtt gtgcacgttg taaaaaacct
    10561 gagcatgtgt agctcagatc cttaccgccg gtttcggttc attctaatga atatatcacc
    10621 cgttactatc gtatttttat gaataatatt ctccgttcaa tttactgatt gtaccctact
    10681 acttatatgt acaatattaa aatgaaaaca atatattgtg ctgaataggt ttatagcgac
    10741 atctatgata gagcgccaca ataacaaaca attgcgtttt attattacaa atccaatttt
    10801 aaaaaaagcg gcagaaccgg tcaaacctaa aagactgatt acataaatct tattcaaatt
    10861 tcaaaagtgc cccaggggct agtatctacg acacaccgag cggcgaacta ataacgctca
    10921 ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga agttgagtat
    10981 tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc acggcggccg
    11041 ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg tgggccccaa
    11101 atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat
    11161 tttgcgacga gctcgaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
    11221 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    11281 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    11341 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    11401 ttggctagag cagcttgcca acatggtgga gcacgacact ctcgtctact ccaagaatat
    11461 caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa gggtaatatc
    11521 gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa ggacagtaga
    11581 aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga
    11641 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
    11701 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgaacatg gtggagcacg
    11761 acactctcgt ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg
    11821 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
    11881 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
    11941 ataaaggaaa ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc
    12001 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
    12061 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
    12121 acccttcctc tatataagga agttcatttc atttggagag gacacgctga aatcaccagt
    12181 ctctctctac aaatctatct ctctcgagtc taccatgagc ccagaacgac gcccggccga
    12241 catccgccgt gccaccgagg cggacatgcc ggcggtctgc accatcgtca accactacat
    12301 cgagacaagc acggtcaact tccgtaccga gccgcaggaa ccgcaggagt ggacggacga
    12361 cctcgtccgt ctgcgggagc gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc
    12421 cggcatcgcc tacgcgggcc cctggaaggc acgcaacgcc tacgactgga cggccgagtc
    12481 gaccgtgtac gtctcccccc gccaccagcg gacgggactg ggctccacgc tctacaccca
    12541 cctgctgaag tccctggagg cacagggctt caagagcgtg gtcgctgtca tcgggctgcc
    12601 caacgacccg agcgtgcgca tgcacgaggc gctcggatat gccccccgcg gcatgctgcg
    12661 ggcggccggc ttcaagcacg ggaactggca tgacgtgggt ttctggcagc tggacttcag
    12721 cctgccggta ccgccccgtc cggtcctgcc cgtcaccgag atttgactcg agtttctcca
    12781 taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat
    12841 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
    12901 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcccc cgaattaatt
    12961 cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
    13021 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
    13081 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
    13141 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
    13201 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
    13261 atgacagagc gttgctgcct gtgatcaccg cggtttcaaa atcggctccg tcgatactat
    13321 gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta
    13381 gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt
    13441 taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa
    13501 ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct
    13561 aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg
    13621 tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga
    13681 aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc
    13741 atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa
    13801 aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat
    13861 tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac
    13921 gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc
    13981 gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc
    14041 gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt
    14101 gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg
    14161 gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
    14221 gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta cctagaatgc
    14281 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    14341 atcaaaggat cttc
//
