LOCUS       sequence-401487-       15818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express SUVH9-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15818)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Li Z, Feng S, Kuo HY, Kan RL, 
            Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, 
            Wohlschlegel J, Jacobsen SE
  TITLE     A gene silencing screen uncovers diverse tools for targeted gene 
            repression in Arabidopsis.
  JOURNAL   Nat Plants. 2023 Mar 6. doi: 10.1038/s41477-023-01362-8.
  PUBMED    36879017
REFERENCE   2  (bases 1 to 15818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2023 Mar 6. doi: 10.1038/s41477-023-01362-8."
COMMENT     Sequence Label: pMDC123-SUVH9-ZF
FEATURES             Location/Qualifiers
     source          1..15818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1077..1868)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2276..2470
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2536..3609)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4038..4667)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    5968..5992
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6180..6202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6194..6211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6195..6211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8309..8353
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8381..8404
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8915..8986
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    9026..9050
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11040..11064)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11876..11892)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11876..11892)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11889..11911)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11900..11916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11924..11930,11931..11948,11949..11954))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11969..11990
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12181..12857
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12794..12814
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             12902..13453
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    13460..13634
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13712..13736
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     mobile_element  14148..14915
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             14938..15732
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccttat
     1081 ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct
     1141 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     1201 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     1261 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     1321 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     1381 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     1441 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     1501 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     1561 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     1621 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     1681 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     1741 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     1801 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     1861 tccctcataa tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     1921 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     1981 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     2041 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatggc gccggcggtc
     2101 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     2161 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     2221 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     2281 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     2341 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     2401 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     2461 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     2521 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     2581 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     2641 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     2701 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     2761 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     2821 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     2881 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     2941 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     3001 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     3061 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     3121 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     3181 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     3241 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     3301 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     3361 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
     3421 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     3481 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     3541 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
     3601 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcaggca
     3661 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
     3721 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
     3781 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
     3841 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
     3901 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
     3961 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
     4021 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
     4081 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
     4141 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
     4201 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
     4261 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
     4321 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
     4381 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
     4441 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
     4501 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
     4561 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
     4621 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
     4681 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
     4741 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
     4801 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
     4861 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
     4921 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
     4981 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
     5041 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
     5101 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
     5161 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
     5221 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
     5281 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
     5341 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
     5401 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
     5461 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
     5521 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
     5581 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
     5641 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
     5701 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
     5761 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
     5821 actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac cctgtggttg
     5881 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt
     5941 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
     6001 tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagcattc
     6061 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     6121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     6181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtcgactc
     6241 tagaggatcc ccgggtaccg ggccccccct cgaggcgcgc cacgcgtagt ctagctcaac
     6301 agagctttta acccaaattg gtacaataga atacaacttt agatcataat tctcaaaaga
     6361 aagagattcc ttagctattc tatctgccac tccatttcct tctcggcttg tatgcacaag
     6421 cataaaatcc tcaaacttgc taagtagata ctttatgtct tggataattg gattgagact
     6481 tgacaagcat aactttcatg taaccaaaga cacaagttgc tgagaatcca cctcaaaaat
     6541 gatcttccta taattgaatc gggataatga cagcacagcc catctaagag cctccacttc
     6601 tacttccagc acgcttctta cttttaccac agctcttgca cctaaccata acaccttccc
     6661 tgtatgatcg cgaagcaccc accctaagcc acattttaat ccttctgttg gccatgcccc
     6721 atcaaagttg cacttaaccc aagattgtgg tggagcttcc catgtttctc gtctgtcccg
     6781 acggtgttgt ggttggtgct ttccttacat tctgagcctc tttccttcta atccactcat
     6841 ctgcatcttc ttgtgtcctt actaatacct cattggttcc aaattccctc cctttaagca
     6901 ccagctcgtt tctgttcttc cacagcctcc caagtatcca agggactaaa gcctccacat
     6961 tcttcagatc aggatattct tgtttaagat gttgaactct atggaggttt gtatgaactg
     7021 atgatctagg accggataag ttcccttctt catagcgaac ttattcaaag aatgttttgt
     7081 gtatcattct tgttacattg ttattaatga aaaaatatta ttggtcattg gactgaacac
     7141 gagtgttaaa tatggaccag gccccaaata agatccattg atatatgaat taaataacaa
     7201 gaataaatcg agtcaccaaa ccacttgcct tttttaacga gacttgttca ccaacttgat
     7261 acaaaagtca ttatcctatg caaatcaata atcatacaaa aatatccaat aacactaaaa
     7321 aattaaaaga aatggataat ttcacaatat gttatacgat aaagaagtta cttttccaag
     7381 aaattcactg attttataag cccacttgca ttagataaat ggcaaaaaaa aacaaaaagg
     7441 aaaagaaata aagcacgaag aattctagaa aatacgaaat acgcttcaat gcagtgggac
     7501 ccacggttca attattgcca attttcagct ccaccgtata tttaaaaaat aaaacgataa
     7561 tgctaaaaaa atataaatcg taacgatcgt taaatctcaa cggctggatc ttatgacgac
     7621 cgttagaaat tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg gttgcagccg
     7681 gcacacacga gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa cctaaaaata
     7741 aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt gtcattttat
     7801 tattagctat tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc gtcttttctt
     7861 cttcttcttc tataaaacaa tacccaaaga gctcttcttc ttcacaattc agatttcaat
     7921 ttctcaaaat cttaaaaact ttctctcaat tctctctacc gtgatcaagg taaatttctg
     7981 tgttccttat tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat ttcgtatatg
     8041 ttctttggtt tagattctgt taatcttaga tcgaagacga ttttctgggt ttgatcgtta
     8101 gatatcatct taattctcga ttagggtttc atagatatca tccgatttgt tcaaataatt
     8161 tgagttttgt cgaataatta ctcttcgatt tgtgatttct atctagatct ggtgttagtt
     8221 tctagtttgt gcgatcgaat ttgtcgatta atctgagttt ttctgattaa cagacgcgtc
     8281 catggttggg taccaacaaa tggctggtgg acttaacgat atcttcgaag ctcagaagat
     8341 tgaatggcat gaggatactg gtggatctag catccccgga cttgaggttc ttttccaagg
     8401 acctctcgag ccttataaat gccctgagtg tggaaaatct ttctctcaat ctggagatct
     8461 taggaggcac caaaggaccc ataccggtga aaaaccttat aaatgccctg agtgtggaaa
     8521 atcattcagt aggagtgatc atcttaccac ccatcagagg acccatactg gtgaaaaacc
     8581 ttataaatgc cctgagtgtg gaaaatcatt ctcacaatct tcatctcttg ttaggcatca
     8641 gaggactcat accggagaaa aaccttataa atgccctgag tgtggaaaat cattctcaac
     8701 atctggaaac cttgttaggc accaaaggac ccatactggt gaaaaacctt ataaatgccc
     8761 tgagtgtgga aaatcattct ctcagagagc aaaccttagg gctcatcaaa ggacacacac
     8821 tggagaaaaa ccttataaat gccctgagtg tggaaaatct ttctctagga gtgataagct
     8881 caccgagcat caaaggactc acaccggact cgaggactac aaagacgatg acgacaaaga
     8941 ctacaaagac gatgacgaca aagactacaa agacgatgac gacaaaaagc ttgatatcgc
     9001 catggggggc gcgccaagct atcaaacaag tttgtacaaa aaagcaggct ccgcggccgc
     9061 ccccttcacc atgggttctt ctcacattcc tcttgatccg tctctaaatc cgtcaccttc
     9121 actgatccca aagcttgaac cagtcactga atcaacccaa aacttggcct ttcaacttcc
     9181 aaacacaaac ccacaagccc taatttcatc agccgtctcc gatttcaacg aagccacaga
     9241 cttttcctca gattacaaca ccgtcgccga gtcagcccgg tctgctttcg ctcaacggct
     9301 tcaacgtcac gatgatgttg cggttcttga ttccttaacc ggagcaatcg taccggttga
     9361 ggagaatccg gaaccggaac cgaatcctta ctcaaccagt gactcttcac cgtcggttgc
     9421 tactcaacga cctagaccgc agccacgttc gtcggagcta gtgaggatca ctgatgttgg
     9481 acctgaaagt gagagacagt ttcgtgaaca tgtgaggaag acgagaatga tttatgattc
     9541 tcttaggatg tttttgatga tggaagaagc taaacgtaat ggggttggtg gaagaagagc
     9601 tagagctgat ggtaaagctg gtaaagctgg ttcaatgatg agagattgta tgttgtggat
     9661 gaatcgtgat aaacgaatcg tcggttcgat tcccggtgtt caagttgggg atatcttctt
     9721 ctttaggttt gagttatgtg tcatgggctt acatggacac cctcaatctg ggatcgattt
     9781 tcttacaggg agtcttagtt ctaatgggga gccaatagct actagtgtga ttgtttctgg
     9841 tgggtatgag gatgatgatg atcaaggaga tgtgattatg tatacaggtc agggtggaca
     9901 agataggctt ggaaggcaag ctgaacatca gaggctggaa ggtggaaacc ttgcgatgga
     9961 acggagtatg tattatggga ttgaagtgag agttattaga gggttgaagt atgagaatga
    10021 ggtttctagt agagtttatg tttatgatgg gttgtttagg attgttgatt cttggtttga
    10081 tgttgggaag tctggtttcg gtgtgtttaa atatcggttg gagaggattg aaggacaggc
    10141 tgagatgggt agttcggtgt tgaagtttgc taggactctt aagactaatc cattgtctgt
    10201 gagaccgaga ggttacatca atttcgatat ctcgaatggg aaggagaatg ttcctgtcta
    10261 tttgtttaac gacattgaca gcgatcaaga acctttgtat tatgagtatc ttgcgcaaac
    10321 ttcgtttcct cctggcttat ttgttcagca aagtggtaat gcaagtggat gtgactgtgt
    10381 caatggttgt ggcagtggct gcctttgtga agccaagaat tcaggtgaga ttgcttatga
    10441 ttataatggg acgctaataa gacagaaacc gttgatacat gaatgtggat cagcgtgtca
    10501 gtgccctcca agctgtcgaa accgtgtgac tcaaaagggt ttgaggaata ggctagaagt
    10561 gtttaggtca ctggaaacgg gttggggagt tcggtctttg gatgtattac atgctggtgc
    10621 ttttatttgt gagtatgctg gggttgcttt gacaagggaa caagccaaca ttctgaccat
    10681 gaacggtgat acattggtat atcctgctcg tttctcttct gcgagatggg aagattgggg
    10741 agatctgtct caggtccttg ctgatttcga gcggccttct tatcctgata ttcctccggt
    10801 tgatttcgca atggatgtgt ccaagatgag gaatgttgct tgttacataa gccacagtac
    10861 tgatccgaat gtcattgtcc agtttgtact ccatgatcac aacagtctca tgttccctag
    10921 agtcatgctt ttcgctgctg aaaacatccc tcccatgact gagctcagcc ttgattacgg
    10981 agtagttgat gattggaacg ccaagcttgc catttgtaat taaaagggtg ggcgcgccga
    11041 cccagctttc ttgtacaaag tggttcgata attcttaatt aactagttct agagcggccg
    11101 ccaccgcggt ggagctccta ggtgagtcta gagagttaat taagacccgg gactagtccc
    11161 tagagtcctg ctttaatgag atatgcgaga cgcctatgat cgcatgatat ttgctttcaa
    11221 ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt
    11281 tcggttcatt ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc
    11341 cgttcaattt actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata
    11401 tattgtgctg aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt
    11461 gcgttttatt attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag
    11521 actgattaca taaatcttat tcaaatttca aaagtgcccc aggggctagt atctacgaca
    11581 caccgagcgg cgaactaata acgctcactg aagggaactc cggttccccg ccggcgcgca
    11641 tgggtgagat tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc
    11701 attcaacccg gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag
    11761 catttttttg gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac
    11821 aaatcgttgg gcgggtccag ggcgaatttt gcgacgagct cgaattcgta atcatgtcat
    11881 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    11941 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    12001 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    12061 aacgcgcggg gagaggcggt ttgcgtattg gctagagcag cttgccaaca tggtggagca
    12121 cgacactctc gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat
    12181 tgagactttt caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat
    12241 ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg
    12301 cgataaagga aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc
    12361 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
    12421 ggattgatgt gaacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
    12481 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
    12541 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
    12601 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg
    12661 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
    12721 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
    12781 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
    12841 tggagaggac acgctgaaat caccagtctc tctctacaaa tctatctctc tcgagtctac
    12901 catgagccca gaacgacgcc cggccgacat ccgccgtgcc accgaggcgg acatgccggc
    12961 ggtctgcacc atcgtcaacc actacatcga gacaagcacg gtcaacttcc gtaccgagcc
    13021 gcaggaaccg caggagtgga cggacgacct cgtccgtctg cgggagcgct atccctggct
    13081 cgtcgccgag gtggacggcg aggtcgccgg catcgcctac gcgggcccct ggaaggcacg
    13141 caacgcctac gactggacgg ccgagtcgac cgtgtacgtc tccccccgcc accagcggac
    13201 gggactgggc tccacgctct acacccacct gctgaagtcc ctggaggcac agggcttcaa
    13261 gagcgtggtc gctgtcatcg ggctgcccaa cgacccgagc gtgcgcatgc acgaggcgct
    13321 cggatatgcc ccccgcggca tgctgcgggc ggccggcttc aagcacggga actggcatga
    13381 cgtgggtttc tggcagctgg acttcagcct gccggtaccg ccccgtccgg tcctgcccgt
    13441 caccgagatt tgactcgagt ttctccataa taatgtgtga gtagttccca gataagggaa
    13501 ttagggttcc tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat
    13561 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagta
    13621 ctaaaatcca gatcccccga attaattcgg cgttaattca gtacattaaa aacgtccgca
    13681 atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag
    13741 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
    13801 catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
    13861 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
    13921 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg
    13981 tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca actttgaaaa
    14041 agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg agttcgtctt
    14101 gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggggt gatgctgcca
    14161 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
    14221 agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga
    14281 catcagcgct atctctgctc tcactgccgt aaaacatggc aactgcagtt cacttacacc
    14341 gcttctcaac ccggtacgca ccagaaaatc attgatatgg ccatgaatgg cgttggatgc
    14401 cgggcaacag cccgcattat gggcgttggc ctcaacacga ttttacgtca cttaaaaaac
    14461 tcaggccgca gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg
    14521 aaatggacga acagtggggc tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg
    14581 cgtatgacag tctccggaag acggttgttg cgcacgtatt cggtgaacgc actatggcga
    14641 cgctggggcg tcttatgagc ctgctgtcac cctttgacgt ggtgatatgg atgacggatg
    14701 gctggccgct gtatgaatcc cgcctgaagg gaaagctgca cgtaatcagc aagcgatata
    14761 cgcagcgaat tgagcggcat aacctgaatc tgaggcagca cctggcacgg ctgggacgga
    14821 agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg cattatctga
    14881 acataaaaca ctatcaataa gttggagtca ttaccgaaaa gaggaaggaa ataataaatg
    14941 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
    15001 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
    15061 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
    15121 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
    15181 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
    15241 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
    15301 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc
    15361 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
    15421 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
    15481 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
    15541 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
    15601 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
    15661 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
    15721 gaattgtttt agtacctaga atgcatgacc aaaatccctt aacgtgagtt ttcgttccac
    15781 tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
