LOCUS       sequence-401488-       16382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express SUVH2-ZF108 in Arabidopsis target FWA gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16382)
  AUTHORS   Wang M, Zhong Z, Gallego-Bartolome J, Li Z, Feng S, Kuo HY, Kan RL, 
            Lam H, Richey JC, Tang L, Zhou J, Liu M, Jami-Alahmadi Y, 
            Wohlschlegel J, Jacobsen SE
  TITLE     A gene silencing screen uncovers diverse tools for targeted gene 
            repression in Arabidopsis.
  JOURNAL   Nat Plants. 2023 Mar 6. doi: 10.1038/s41477-023-01362-8.
  PUBMED    36879017
REFERENCE   2  (bases 1 to 16382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2023 Mar 6. doi: 10.1038/s41477-023-01362-8."
COMMENT     Sequence Label: pMDC123-SUVH2-ZF
FEATURES             Location/Qualifiers
     source          1..16382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1077..1868)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2276..2470
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2536..3609)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4038..4667)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    5968..5992
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6180..6202
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6194..6211
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6195..6211
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8309..8353
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8381..8404
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8915..8986
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    9026..9050
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(11043..11067)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(11879..11895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11879..11895)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11892..11914)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11903..11919
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11927..11933,11934..11951,11952..11957))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11972..11993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12184..12860
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12797..12817
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             12905..13456
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    13463..13637
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13715..13739
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14164..14958
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccttat
     1081 ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct
     1141 gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     1201 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc
     1261 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     1321 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     1381 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     1441 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     1501 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     1561 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     1621 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     1681 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     1741 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     1801 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     1861 tccctcataa tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     1921 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     1981 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     2041 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatggc gccggcggtc
     2101 gagtggcgac ggcgcggctt gtccgcgccc tggtagattg cctggccgta ggccagccat
     2161 ttttgagcgg ccagcggccg cgataggccg acgcgaagcg gcggggcgta gggagcgcag
     2221 cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg cgctggccag acagttatgc
     2281 acaggccagg cgggttttaa gagttttaat aagttttaaa gagttttagg cggaaaaatc
     2341 gccttttttc tcttttatat cagtcactta catgtgtgac cggttcccaa tgtacggctt
     2401 tgggttccca atgtacgggt tccggttccc aatgtacggc tttgggttcc caatgtacgt
     2461 gctatccaca ggaaagagac cttttcgacc tttttcccct gctagggcaa tttgccctag
     2521 catctgctcc gtacattagg aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg
     2581 catgactagg atcgggccag cctgccccgc ctcctccttc aaatcgtact ccggcaggtc
     2641 atttgacccg atcagcttgc gcacggtgaa acagaacttc ttgaactctc cggcgctgcc
     2701 actgcgttcg tagatcgtct tgaacaacca tctggcttct gccttgcctg cggcgcggcg
     2761 tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac
     2821 cgtcagcacg tccgggttct tgccttctgt gatctcgcgg tacatccaat cagctagctc
     2881 gatctcgatg tactccggcc gcccggtttc gctctttacg atcttgtagc ggctaatcaa
     2941 ggcttcaccc tcggataccg tcaccaggcg gccgttcttg gccttcttcg tacgctgcat
     3001 ggcaacgtgc gtggtgttta accgaatgca ggtttctacc aggtcgtctt tctgctttcc
     3061 gccatcggct cgccggcaga acttgagtac gtccgcaacg tgtggacgga acacgcggcc
     3121 gggcttgtct cccttccctt cccggtatcg gttcatggat tcggttagat gggaaaccgc
     3181 catcagtacc aggtcgtaat cccacacact ggccatgccg gccggccctg cggaaacctc
     3241 tacgtgcccg tctggaagct cgtagcggat cacctcgcca gctcgtcggt cacgcttcga
     3301 cagacggaaa acggccacgt ccatgatgct gcgactatcg cgggtgccca cgtcatagag
     3361 catcggaacg aaaaaatctg gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc
     3421 accggctgcc ggcggttgcc gggattcttt gcggattcga tcagcggccg cttgccacga
     3481 ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa
     3541 cttctccacc aggtcatcac ccagcgccgc gccgatttgt accgggccgg atggtttgcg
     3601 accgctcacg ccgattcctc gggcttgggg gttccagtgc cattgcaggg ccggcaggca
     3661 acccagccgc ttacgcctgg ccaaccgccc gttcctccac acatggggca ttccacggcg
     3721 tcggtgcctg gttgttcttg attttccatg ccgcctcctt tagccgctaa aattcatcta
     3781 ctcatttatt catttgctca tttactctgg tagctgcgcg atgtattcag atagcagctc
     3841 ggtaatggtc ttgccttggc gtaccgcgta catcttcagc ttggtgtgat cctccgccgg
     3901 caactgaaag ttgacccgct tcatggctgg cgtgtctgcc aggctggcca acgttgcagc
     3961 cttgctgctg cgtgcgctcg gacggccggc acttagcgtg tttgtgcttt tgctcatttt
     4021 ctctttacct cattaactca aatgagtttt gatttaattt cagcggccag cgcctggacc
     4081 tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg
     4141 cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa tgggcagctc gtacccggcc
     4201 agcgcctcgg caacctcacc gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag
     4261 gccgcttgta gccttccatc cgtgacctca atgcgctgct taaccagctc caccaggtcg
     4321 gcggtggccc atatgtcgta agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc
     4381 ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg
     4441 cgccggccga tggccttcac gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt
     4501 agcggttgat cttcccgcac ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg
     4561 actaacagaa catcggcccc ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc
     4621 gtcttgcctg acccgccttt ctggttaagt acagcgataa ccttcatgcg ttccccttgc
     4681 gtatttgttt atttactcat cgcatcatat acgcagcgac cgcatgacgc aagctgtttt
     4741 actcaaatac acatcacctt tttagacggc ggcgctcggt ttcttcagcg gccaagctgg
     4801 ccggccaggc cgccagcttg gcatcagaca aaccggccag gatttcatgc agccgcacgg
     4861 ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc gatcatctcc gcctcgatct
     4921 cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc
     4981 ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa
     5041 ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta
     5101 cagggtcgag cgatgcacgc caagcagtgc agccgcctct ttcacggtgc ggccttcctg
     5161 gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg agggtagggc gggggccaaa
     5221 cttcacgcct cgggccttgg cggcctcgcg cccgctccgg gtgcggtcga tgattaggga
     5281 acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt
     5341 ggtgtcggcc cacggctctg ccaggctacg caggcccgcg ccggcctcct ggatgcgctc
     5401 ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac
     5461 aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc tccgggcggt cgcgcctggt
     5521 gccggtgatc ttctcggaaa acagcttggt gcagccggcc gcgtgcagtt cggcccgttg
     5581 gttggtcaag tcctggtcgt cggtgctgac gcgggcatag cccagcaggc cagcggcggc
     5641 gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa gtattctact ttatgcgact
     5701 aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta
     5761 ggacttgtgc gacatgtcgt tttcagaaga cggctgcact gaacgtcaga agccgactgc
     5821 actatagcag cggaggggtt ggatcaaagt actttgatcc cgaggggaac cctgtggttg
     5881 gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa atatccgatt
     5941 attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt caaacactga
     6001 tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc tctagcattc
     6061 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     6121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     6181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtcgactc
     6241 tagaggatcc ccgggtaccg ggccccccct cgaggcgcgc cacgcgtagt ctagctcaac
     6301 agagctttta acccaaattg gtacaataga atacaacttt agatcataat tctcaaaaga
     6361 aagagattcc ttagctattc tatctgccac tccatttcct tctcggcttg tatgcacaag
     6421 cataaaatcc tcaaacttgc taagtagata ctttatgtct tggataattg gattgagact
     6481 tgacaagcat aactttcatg taaccaaaga cacaagttgc tgagaatcca cctcaaaaat
     6541 gatcttccta taattgaatc gggataatga cagcacagcc catctaagag cctccacttc
     6601 tacttccagc acgcttctta cttttaccac agctcttgca cctaaccata acaccttccc
     6661 tgtatgatcg cgaagcaccc accctaagcc acattttaat ccttctgttg gccatgcccc
     6721 atcaaagttg cacttaaccc aagattgtgg tggagcttcc catgtttctc gtctgtcccg
     6781 acggtgttgt ggttggtgct ttccttacat tctgagcctc tttccttcta atccactcat
     6841 ctgcatcttc ttgtgtcctt actaatacct cattggttcc aaattccctc cctttaagca
     6901 ccagctcgtt tctgttcttc cacagcctcc caagtatcca agggactaaa gcctccacat
     6961 tcttcagatc aggatattct tgtttaagat gttgaactct atggaggttt gtatgaactg
     7021 atgatctagg accggataag ttcccttctt catagcgaac ttattcaaag aatgttttgt
     7081 gtatcattct tgttacattg ttattaatga aaaaatatta ttggtcattg gactgaacac
     7141 gagtgttaaa tatggaccag gccccaaata agatccattg atatatgaat taaataacaa
     7201 gaataaatcg agtcaccaaa ccacttgcct tttttaacga gacttgttca ccaacttgat
     7261 acaaaagtca ttatcctatg caaatcaata atcatacaaa aatatccaat aacactaaaa
     7321 aattaaaaga aatggataat ttcacaatat gttatacgat aaagaagtta cttttccaag
     7381 aaattcactg attttataag cccacttgca ttagataaat ggcaaaaaaa aacaaaaagg
     7441 aaaagaaata aagcacgaag aattctagaa aatacgaaat acgcttcaat gcagtgggac
     7501 ccacggttca attattgcca attttcagct ccaccgtata tttaaaaaat aaaacgataa
     7561 tgctaaaaaa atataaatcg taacgatcgt taaatctcaa cggctggatc ttatgacgac
     7621 cgttagaaat tgtggttgtc gacgagtcag taataaacgg cgtcaaagtg gttgcagccg
     7681 gcacacacga gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa cctaaaaata
     7741 aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt gtcattttat
     7801 tattagctat tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc gtcttttctt
     7861 cttcttcttc tataaaacaa tacccaaaga gctcttcttc ttcacaattc agatttcaat
     7921 ttctcaaaat cttaaaaact ttctctcaat tctctctacc gtgatcaagg taaatttctg
     7981 tgttccttat tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat ttcgtatatg
     8041 ttctttggtt tagattctgt taatcttaga tcgaagacga ttttctgggt ttgatcgtta
     8101 gatatcatct taattctcga ttagggtttc atagatatca tccgatttgt tcaaataatt
     8161 tgagttttgt cgaataatta ctcttcgatt tgtgatttct atctagatct ggtgttagtt
     8221 tctagtttgt gcgatcgaat ttgtcgatta atctgagttt ttctgattaa cagacgcgtc
     8281 catggttggg taccaacaaa tggctggtgg acttaacgat atcttcgaag ctcagaagat
     8341 tgaatggcat gaggatactg gtggatctag catccccgga cttgaggttc ttttccaagg
     8401 acctctcgag ccttataaat gccctgagtg tggaaaatct ttctctcaat ctggagatct
     8461 taggaggcac caaaggaccc ataccggtga aaaaccttat aaatgccctg agtgtggaaa
     8521 atcattcagt aggagtgatc atcttaccac ccatcagagg acccatactg gtgaaaaacc
     8581 ttataaatgc cctgagtgtg gaaaatcatt ctcacaatct tcatctcttg ttaggcatca
     8641 gaggactcat accggagaaa aaccttataa atgccctgag tgtggaaaat cattctcaac
     8701 atctggaaac cttgttaggc accaaaggac ccatactggt gaaaaacctt ataaatgccc
     8761 tgagtgtgga aaatcattct ctcagagagc aaaccttagg gctcatcaaa ggacacacac
     8821 tggagaaaaa ccttataaat gccctgagtg tggaaaatct ttctctagga gtgataagct
     8881 caccgagcat caaaggactc acaccggact cgaggactac aaagacgatg acgacaaaga
     8941 ctacaaagac gatgacgaca aagactacaa agacgatgac gacaaaaagc ttgatatcgc
     9001 catggggggc gcgccaagct atcaaacaag tttgtacaaa aaagcaggct ccgcggccgc
     9061 ccccttcacc atgagtacat tgttaccatt tcctgacctc aacctcatgc cggattctca
     9121 atcctccacc gccggaacca cagccggcga cactgtagtc accggaaagt tagaagtgaa
     9181 atcggaacca attgaggaat ggcaaactcc accatcgtct acctccgatc aatcagccaa
     9241 taccgatctc atcgccgagt ttattcgtat ctcagagctc ttccgctcgg cattcaagcc
     9301 actgcaggtc aaaggattag acggagtctc cgtatacgga ttggattctg gtgccatcgt
     9361 tgctgtaccg gagaaagaaa accgggaatt gattgaaccg cctccaggat ttaaggataa
     9421 tcgagtctcc accgtcgttg tctcgccgaa attcgagaga ccgagagagc tagcgagaat
     9481 tgcaattcta ggtcatgaac aacggaagga actccgacaa gtcatgaaac gaactaggat
     9541 gacttatgag tctcttcgga ttcatctaat ggcggagagt atgaagaatc atgtacttgg
     9601 tcaagggcgt agacggagga gcgatatggc ggctgcgtat ataatgaggg atcggggact
     9661 gtggttgaac tatgataagc acatagttgg tccagtcaca ggggttgaag taggggatat
     9721 attcttctac cgtatggaat tgtgtgtgct gggcttacac ggccagacac aagccgggat
     9781 tgattgttta acggctgaac ggagcgccac tggagagcct atagctacca gcattgttgt
     9841 ctcaggtggt tatgaggatg atgaagatac aggagatgtt ttggtttata ctggtcacgg
     9901 tggtcaggat catcaacaca agcagtgtga taaccagagg ctagtaggtg ggaatctggg
     9961 aatggagaga agtatgcact acggtatcga ggtacgtgtg attagaggta ttaagtatga
    10021 gaacagtata agctcgaagg tgtatgtata tgatggtttg tataagatag tagattggtg
    10081 gtttgctgtg gggaagtctg gttttggggt ttttaaattt aggttagtaa gaattgaagg
    10141 gcaaccgatg atgggcagtg cggtaatgag gtttgcacaa actcttagaa ataagccatc
    10201 gatggttagg cctactggtt atgttagctt tgacctttct aacaagaagg agaatgtacc
    10261 cgtgtttcta tataatgatg tagatggtga tcaagaaccg agacattatg agtacattgc
    10321 aaaagctgtc tttcctcctg gaatttttgg tcaggggggg atcagcagga ctggctgcga
    10381 gtgtaagctc tcttgtactg atgattgtct ctgtgcaagg aagaacggtg gtgagtttgc
    10441 atatgatgat aacgggcatc tattgaaagg aaagcatgtg gtatttgaat gtggggaatt
    10501 ctgcacttgt ggtccgagct gtaagagccg tgtgacacag aagggattga ggaacaggct
    10561 agaggttttc agatcgaagg aaaccggttg gggtgtcagg acacttgatc taattgaagc
    10621 tggtgctttc atatgcgaat atgcaggtgt agttgtcacg agacttcaag ccgagattct
    10681 gtcaatgaat ggggatgtta tggtctatcc tggtcggttc acagaccaat ggcgtaactg
    10741 gggtgattta tctcaagtat acccagattt tgttaggccg aattatccat ctctccctcc
    10801 actggatttc tcaatggatg tgtcaaggat gaggaacgtg gcttgctaca ttagtcacag
    10861 caaagaacca aatgtgatgg tgcaatttgt tttgcatgac cacaatcacc tgatgttccc
    10921 ccgtgtgatg ctttttgcgc tggagaatat ctcaccgttg gccgagctaa gcctggatta
    10981 cggcttggca gatgaagtga acggcaagct cgccatctgc aactagaagg gtgggcgcgc
    11041 cgacccagct ttcttgtaca aagtggttcg ataattctta attaactagt tctagagcgg
    11101 ccgccaccgc ggtggagctc ctaggtgagt ctagagagtt aattaagacc cgggactagt
    11161 ccctagagtc ctgctttaat gagatatgcg agacgcctat gatcgcatga tatttgcttt
    11221 caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc cttaccgccg
    11281 gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat gaataatatt
    11341 ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa aatgaaaaca
    11401 atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca ataacaaaca
    11461 attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg tcaaacctaa
    11521 aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct agtatctacg
    11581 acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc ccgccggcgc
    11641 gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa agttacgggc
    11701 accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc gagaattatg
    11761 cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct tgacagtgac
    11821 gacaaatcgt tgggcgggtc cagggcgaat tttgcgacga gctcgaattc gtaatcatgt
    11881 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    11941 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    12001 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    12061 gccaacgcgc ggggagaggc ggtttgcgta ttggctagag cagcttgcca acatggtgga
    12121 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc
    12181 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
    12241 tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca
    12301 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg
    12361 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
    12421 agtggattga tgtgaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag
    12481 atacagtctc agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa
    12541 acctcctcgg attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg
    12601 aaggtggcac ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct
    12661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
    12721 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
    12781 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
    12841 atttggagag gacacgctga aatcaccagt ctctctctac aaatctatct ctctcgagtc
    12901 taccatgagc ccagaacgac gcccggccga catccgccgt gccaccgagg cggacatgcc
    12961 ggcggtctgc accatcgtca accactacat cgagacaagc acggtcaact tccgtaccga
    13021 gccgcaggaa ccgcaggagt ggacggacga cctcgtccgt ctgcgggagc gctatccctg
    13081 gctcgtcgcc gaggtggacg gcgaggtcgc cggcatcgcc tacgcgggcc cctggaaggc
    13141 acgcaacgcc tacgactgga cggccgagtc gaccgtgtac gtctcccccc gccaccagcg
    13201 gacgggactg ggctccacgc tctacaccca cctgctgaag tccctggagg cacagggctt
    13261 caagagcgtg gtcgctgtca tcgggctgcc caacgacccg agcgtgcgca tgcacgaggc
    13321 gctcggatat gccccccgcg gcatgctgcg ggcggccggc ttcaagcacg ggaactggca
    13381 tgacgtgggt ttctggcagc tggacttcag cctgccggta ccgccccgtc cggtcctgcc
    13441 cgtcaccgag atttgactcg agtttctcca taataatgtg tgagtagttc ccagataagg
    13501 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
    13561 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
    13621 gtactaaaat ccagatcccc cgaattaatt cggcgttaat tcagtacatt aaaaacgtcc
    13681 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
    13741 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
    13801 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
    13861 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
    13921 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaccg
    13981 cggtttcaaa atcggctccg tcgatactat gttatacgcc aactttgaaa acaactttga
    14041 aaaagctgtt ttctggtatt taaggtttta gaatgcaagg aacagtgaat tggagttcgt
    14101 cttgttataa ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa
    14161 taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc
    14221 gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa
    14281 aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg
    14341 gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt
    14401 gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg
    14461 gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc
    14521 aggctctttc actccatcga catatcggat tgtccctata cgaatagctt agacagccgc
    14581 ttagccgaat tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg
    14641 gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag
    14701 cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa
    14761 gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat
    14821 gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag
    14881 ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta
    14941 ctggatgaat tgttttagta cctagaatgc atgaccaaaa tcccttaacg tgagttttcg
    15001 ttccactgag cgctgatgaa tcccctaatg atttttatca aaatcattaa gttaaggtag
    15061 atacacatct tgtcatatga tcaaatggtt tcgccaaaaa tcaataatca gacaacaaaa
    15121 tgtgcgaact cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa
    15181 aacgactcaa cagcttaacg ttggcttgcc acgccttact tgactgtaaa actctcactc
    15241 ttaccgaact tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa
    15301 tcgaccgatt gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc
    15361 atgctagctt tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata
    15421 tccgtgagca aaaacggctt atggtattgc gagcttcagt cgcactacac ggtcgttctg
    15481 ttactcttta tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc
    15541 aatttctagc cgaccttgcg agcattctac cgagtaacac cacaccgctc attgtcagtg
    15601 atgctggctt taaagtgcca tggtataaat ccgttgagaa gctgggttgg tactggttaa
    15661 gtcgagtaag aggaaaagta caatatgcag acctaggagc ggaaaactgg aaacctatca
    15721 gcaacttaca tgatatgtca tctagtcact caaagacttt aggctataag aggctgacta
    15781 aaagcaatcc aatctcatgc caaattctat tgtataaatc tcgctctaaa ggccgaaaaa
    15841 atcagcgctc gacacggact cattgtcacc acccgtcacc taaaatctac tcagcgtcgg
    15901 caaaggagcc atggattcta gcaactaact tacctgttga aattcgaaca cccaaacaac
    15961 ttgttaatat ctattcgaag cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc
    16021 ctgcctacgg actaggccta cgccatagcc gaacgagcag ctcagagcgt tttgatatca
    16081 tgctgctaat cgccctgatg cttcaactaa catgttggct tgcgggcgtt catgctcaga
    16141 aacaaggttg ggacaagcac ttccaggcta acacagtcag aaatcgaaac gtactctcaa
    16201 cagttcgctt aggcatggaa gttttgcggc attctggcta cacaataaca agggaagact
    16261 tactcgtggc tgcaacccta ctagctcaaa atttattcac acatggttac gctttgggga
    16321 aattatgagg ggatctctca gcactgagcg tcagaccccg tagaaaagat caaaggatct
    16381 tc
//
