LOCUS       sequence-401519-        4500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF76 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF76
FEATURES             Location/Qualifiers
     source          1..4500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1811..1910)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1927..1946)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1928..1946)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1951..1967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1951..1967)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2398..3189
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3282..3870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3771..3790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4024..4041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4200..4227)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4319..4405)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4454..4476
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4468..4485
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4469..4485
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggagagctt ggggctgcac
      121 acggtgaccc ttagtgatgg gacaacagcc tacgtccagc aagctgtcaa aggagagaag
      181 cttcttgaag ggcaggtgat ccagctcgag gatgggacca ccgcatacat tcaccaggtg
      241 acggtacaga aagaagctct ctcctttgag gatggtcagc ctgtgcagct ggaagatggc
      301 agcatggctt acatacaccg cacacccaga gaaggctatg accccagcac cctggaagcc
      361 gtccaactgg aagatggctc cactgcctac attcaccacc ctgtggctgt gccatcggag
      421 agcaccatcc tggccgtaca gacagaggtg ggcttggagg acctggcagc agaggatgat
      481 gagggcttca gtgcagacgc agtggtggcc ctggagcagt atgccagcaa ggttcttcat
      541 gacagccaga ttccccgtaa tggaaaaggg cagcaagttg gagacagagc attccgctgt
      601 ggctacaagg gctgtgggcg tctctacacc accgctcatc acttaaaggt gcatgaacga
      661 gctcatacag gtgaccgtcc atacagatgt gacttcccca gctgtggaaa ggcctttgcc
      721 acaggctatg gactgaagag ccacgttcgt acccacactg gtgagaaacc atacaagtgc
      781 ccagaggagc tgtgcagcaa ggccttcaag acctcaggag acctgcagaa gcatgtccgt
      841 acccacactg gtgaacgccc gttccagtgc ccttttgagg gctgtggccg ctccttcacc
      901 acatctaaca tccgcaaggt acatgtgcgc acccacacag gcgagaggcc ctacacctgc
      961 ccggagcccc actgtggccg cggcttcacc agcgccacca actataagaa tcacgtgcgc
     1021 atccacacag gggagaagcc atacgtttgc acggtgccag gctgcgggaa acgcttcacc
     1081 gagtactcga gcttgtataa gcaccacgtg gtgcacacac actgcaagcc ctacacctgc
     1141 agcacctgcg gcaagaccta ccggcagacc tccaccttgg ccatgcacaa gcgcagtgcc
     1201 catggcgagc tggaggccac ggaggagagt gagcaggccc tctatgagca gcagcaactt
     1261 gaggccgcct ctgcagccga ggagagtccg ccacccaaac gaccccggat agcttacctt
     1321 tcggaggtga aggaagagag agatgacatc ccagcccagg tggcgatggt gactgaagaa
     1381 gatggggccc cccaggtggc tctgatcact caggatggtg cccagcaggt cagcctgtcc
     1441 ccggaagacc tgcaggccct ggggagtgcc atcagtatgg tcacccagca cggcagcacc
     1501 accctcacca tccccagtcc tgatgccgac ctggccacat ctggcacaca tacagtcacc
     1561 atggtcagcg ccgatggcac ccagacgcag cccgtcacaa tcattacctc tggggctgtg
     1621 gtggctgagg actcaagtgt agcatctctt cgtcatcaac aggtggcact gttggccaca
     1681 gccaacggaa cgcacattgc agtgcagctg gaggaacagc agaccttaga ggaggccatc
     1741 aatgtggcca ctgcggccat gcagcaaggg gctgtgaccc tggagacaac agtgtcggag
     1801 agtggctgct gcccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1861 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1921 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1981 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
     2041 tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
     2101 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     2161 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     2221 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     2281 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     2341 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
     2401 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     2461 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     2521 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     2581 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     2641 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     2701 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     2761 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     2821 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     2881 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     2941 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     3001 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     3061 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     3121 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     3181 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     3241 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3301 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3361 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3421 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3481 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3541 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3601 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3661 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3721 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3781 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3841 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3901 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3961 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     4021 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     4081 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     4141 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     4201 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     4261 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     4321 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4381 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     4441 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
