LOCUS       sequence-401525-       14499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14499)
  AUTHORS   Wickersham IR, Sullivan HA, Seung HS
  TITLE     Production of glycoprotein-deleted rabies viruses for monosynaptic 
            tracing and high-level gene expression in neurons.
  JOURNAL   Nat Protoc. 2010 Mar;5(3):595-606. doi: 10.1038/nprot.2009.248. Epub
            2010 Mar 4.
  PUBMED    20203674
REFERENCE   2  (bases 1 to 14499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2009.248"; journalName: "Nat Protoc"; date: 
            "2010-03"; volume: "5"; issue: "3"; pages: "595-606"
COMMENT     Sequence Label: cSPBN-4GFP
FEATURES             Location/Qualifiers
     source          1..14499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(90..109)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     300..321
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        658..1037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1038..1241
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1192..1212
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1286..1305
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1286..1304
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4689..4698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4695..4697,4698..4700,4701..5414)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4695..4697,4698..4700,4701..5411)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4740..4761)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5001..5020)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5348..5369
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7163..7174
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12173..12191)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12187..12234
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12257..12273)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12257..12273)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12310..12330)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12310..12328)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12349..12365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12349..12365)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12362..12384)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12373..12389
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12397..12403,12404..12421,12422..12427))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12442..12463
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12580..12597)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12751..13339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12831..12850)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13510..14301,14302..14370))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14133..14152
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14371..14475)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgataag cttgatatcg aattcctgca gcgcgttgac
      661 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      721 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      781 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      841 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      901 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      961 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     1021 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     1081 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     1141 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     1201 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     1261 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1321 agattaagcg tctgatgagt ccgtgaggac gaaacccggc gtaccgggtc acgcttaaca
     1381 accagatcaa agaaaaaaca gacattgtca attgcaaagc aaaaatgtaa cacccctaca
     1441 atggatgccg acaagattgt attcaaagtc aataatcagg tggtctcttt gaagcctgag
     1501 attatcgtgg atcaatatga gtacaagtac cctgccatca aagatttgaa aaagccctgt
     1561 ataaccctag gaaaggctcc cgatttaaat aaagcataca agtcagtttt gtcaggcatg
     1621 agcgccgcca aacttaatcc tgacgatgta tgttcctatt tggcagcggc aatgcagttt
     1681 tttgagggga catgtccgga agactggacc agctatggaa ttgtgattgc acgaaaagga
     1741 gataagatca ccccaggttc tctggtggag ataaaacgta ctgatgtaga agggaattgg
     1801 gctctgacag gaggcatgga actgacaaga gaccccactg tccctgagca tgcgtcctta
     1861 gtcggtcttc tcttgagtct gtataggttg agcaaaatat ccgggcaaaa cactggtaac
     1921 tataagacaa acattgcaga caggatagag cagatttttg agacagcccc ttttgttaaa
     1981 atcgtggaac accatactct aatgacaact cacaaaatgt gtgctaattg gagtactata
     2041 ccaaacttca gatttttggc cggaacctat gacatgtttt tctcccggat tgagcatcta
     2101 tattcagcaa tcagagtggg cacagttgtc actgcttatg aagactgttc aggactggta
     2161 tcatttactg ggttcataaa acaaatcaat ctcaccgcta gagaggcaat actatatttc
     2221 ttccacaaga actttgagga agagataaga agaatgtttg agccagggca ggagacagct
     2281 gttcctcact cttatttcat ccacttccgt tcactaggct tgagtgggaa atctccttat
     2341 tcatcaaatg ctgttggtca cgtgttcaat ctcattcact ttgtaggatg ctatatgggt
     2401 caagtcagat ccctaaatgc aacggttatt gctgcatgtg ctcctcatga aatgtctgtt
     2461 ctagggggct atctgggaga ggaattcttc gggaaaggga catttgaaag aagattcttc
     2521 agagatgaga aagaacttca agaatacgag gcggctgaac tgacaaagac tgacgtagca
     2581 ctggcagatg atggaactgt caactctgac gacgaggact acttttcagg tgaaaccaga
     2641 agtccggagg ctgtttatac tcgaatcatg atgaatggag gtcgactaaa gagatctcac
     2701 atacggagat atgtctcagt cagttccaat catcaagccc gtccaaactc attcgccgag
     2761 tttctaaaca agacatattc gagtgactca taagaagttg aataacaaaa tgccggaaat
     2821 ctacggattg tgtatatcca tcatgaaaaa aactaacacc cctcctttcg aaccatccca
     2881 aacatgagca agatctttgt caatcctagt gctattagag ccggtctggc cgatcttgag
     2941 atggctgaag aaactgttga tctgatcaat agaaatatcg aagacaatca ggctcatctc
     3001 caaggggaac ccatagaggt ggacaatctc cctgaggata tggggcgact tcacctggat
     3061 gatggaaaat cgcccaacca tggtgagata gccaaggtgg gagaaggcaa gtatcgagag
     3121 gactttcaga tggatgaagg agaggatcct agcttcctgt tccagtcata cctggaaaat
     3181 gttggagtcc aaatagtcag acaaatgagg tcaggagaga gatttctcaa gatatggtca
     3241 cagaccgtag aagagattat atcctatgtc gcggtcaact ttcccaaccc tccaggaaag
     3301 tcttcagagg ataaatcaac ccagactact ggccgagagc tcaagaagga gacaacaccc
     3361 actccttctc agagagaaag ccaatcatcg aaagccagga tggcggctca aattgcttct
     3421 ggccctccag cccttgaatg gtcggctacc aatgaagagg atgatctatc agtggaggct
     3481 gagatcgctc accagattgc agaaagtttc tccaaaaaat ataagtttcc ctctcgatcc
     3541 tcagggatac tcttgtataa ttttgagcaa ttgaaaatga accttgatga tatagttaaa
     3601 gaggcaaaaa atgtaccagg tgtgacccgt ttagcccatg acgggtccaa actcccccta
     3661 agatgtgtac tgggatgggt cgctttggcc aactctaaga aattccagtt gttagtcgaa
     3721 tccgacaagc tgagtaaaat catgcaagat gacttgaatc gctatacatc ttgctaaccg
     3781 aacctctccc ctcagtccct ctagacaata aaatccgaga tgtcccaaag tcaacatgaa
     3841 aaaaacaggc aacaccactg ataaaatgaa cctcctacgt aagatagtga aaaaccgcag
     3901 ggacgaggac actcaaaaat cctctcccgc gtcagcccct ctggatgacg atgacttgtg
     3961 gcttccaccc cctgaatacg tcccgctgaa agaacttaca ggcaagaaga acatgaggaa
     4021 cttttgtatc aacggaaggg ttaaagtgtg tagcccgaat ggttactcgt tcaggatcct
     4081 gcggcacatt ctgaaatcat tcgacgagat atattctggg aatcatagga tgatcgggtt
     4141 agtcaaagtg gttattggac tggctttgtc aggatctcca gtccctgagg gcctgaactg
     4201 ggtatacaaa ttgaggagaa cctttatctt ccagtgggct gattccaggg gccctcttga
     4261 aggggaggag ttggaatact ctcaggagat cacttgggat gatgatactg agttcgtcgg
     4321 attgcaaata agagtgattg caaaacagtg tcatatccag ggcagagtct ggtgtatcaa
     4381 catgaacccg agagcatgtc aactatggtc tgacatgtct cttcagacac aaaggtccga
     4441 agaggacaaa gattcctctc tgcttctaga ataatcagat tatatcccgc aaatttatca
     4501 cttgtttacc tctggaggag agaacatatg ggctcaactc caacccttgg gagcaatata
     4561 acaaaaaaca tgttatggtg ccattaaacc gctgcatttc atcaaagtca agttgattac
     4621 ctttacattt tgatcctctt ggatgtgaaa aaaactatta acatccctca aaagaccccg
     4681 ggaaagccgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     4741 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     4801 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     4861 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     4921 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     4981 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     5041 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     5101 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     5161 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     5221 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     5281 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     5341 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     5401 agctgtacaa gtaagctagc ttataaagtg ctgggtcatc taagcttttc agtcgagaaa
     5461 aaaacattag atcagaagaa caactggcaa cacttctcaa cctgagactt acttcaagat
     5521 gctcgatcct ggagaggtct atgatgaccc tattgaccca atcgagttag aggctgaacc
     5581 cagaggaacc cccattgtcc ccaacatctt gaggaactct gactacaatc tcaactctcc
     5641 tttgatagaa gatcctgcta gactaatgtt agaatggtta aaaacaggga atagacctta
     5701 tcggatgact ctaacagaca attgctccag gtctttcaga gttttgaaag attatttcaa
     5761 gaaggtagat ttgggttctc tcaaggtggg cggaatggct gcacagtcaa tgatttctct
     5821 ctggttatat ggtgcccact ctgaatccaa caggagccgg agatgtataa cagacttggc
     5881 ccatttctat tccaagtcgt cccccataga gaagctgttg aatctcacgc taggaaatag
     5941 agggctgaga atccccccag agggagtgtt aagttgcctt gagagggttg attatgataa
     6001 tgcatttgga aggtatcttg ccaacacgta ttcctcttac ttgttcttcc atgtaatcac
     6061 cttatacatg aacgccctag actgggatga agaaaagacc atcctagcat tatggaaaga
     6121 tttaacctca gtggacatcg ggaaggactt ggtaaagttc aaagaccaaa tatggggact
     6181 gctgatcgtg acaaaggact ttgtttactc ccaaagttcc aattgtcttt ttgacagaaa
     6241 ctacacactt atgctaaaag atcttttctt gtctcgcttc aactccttaa tggtcttgct
     6301 ctctccccca gagccccgat actcagatga cttgatatct caactatgcc agctgtacat
     6361 tgctggggat caagtcttgt ctatgtgtgg aaactccggc tatgaagtca tcaaaatatt
     6421 ggagccatat gtcgtgaata gtttagtcca gagagcagaa aagtttaggc ctctcattca
     6481 ttccttggga gactttcctg tatttataaa agacaaggta agtcaacttg aagagacgtt
     6541 cggtccctgt gcaagaaggt tctttagggc tctggatcaa ttcgacaaca tacatgactt
     6601 ggtttttgtg tttggctgtt acaggcattg ggggcaccca tatatagatt atcgaaaggg
     6661 tctgtcaaaa ctatatgatc aggttcacct taaaaaaatg atagataagt cctaccagga
     6721 gtgcttagca agcgacctag ccaggaggat ccttagatgg ggttttgata agtactccaa
     6781 gtggtatctg gattcaagat tcctagcccg agaccacccc ttgactcctt atatcaaaac
     6841 ccaaacatgg ccacccaaac atattgtaga cttggtgggg gatacatggc acaagctccc
     6901 gatcacgcag atctttgaga ttcctgaatc aatggatccg tcagaaatat tggatgacaa
     6961 atcacattct ttcaccagaa cgagactagc ttcttggctg tcagaaaacc gaggggggcc
     7021 tgttcctagc gaaaaagtta ttatcacggc cctgtctaag ccgcctgtca atccccgaga
     7081 gtttctgagg tctatagacc tcggaggatt gccagatgaa gacttgataa ttggcctcaa
     7141 gccaaaggaa cgggaattga agattgaagg tcgattcttt gctctaatgt catggaatct
     7201 aagattgtat tttgtcatca ctgaaaaact cttggccaac tacatcttgc cactttttga
     7261 cgcgctgact atgacagaca acctgaacaa ggtgtttaaa aagctgatcg acagggtcac
     7321 cgggcaaggg cttttggact attcaagggt cacatatgca tttcacctgg actatgaaaa
     7381 gtggaacaac catcaaagat tagagtcaac agaggatgta ttttctgtcc tagatcaagt
     7441 gtttggattg aagagagtgt tttctagaac acacgagttt tttcaaaagg cctggatcta
     7501 ttattcagac agatcagacc tcatcgggtt acgggaggat caaatatact gcttagatgc
     7561 gtccaacggc ccaacctgtt ggaatggcca ggatggcggg ctagaaggct tacggcagaa
     7621 gggctggagt ctagtcagct tattgatgat agatagagaa tctcaaatca ggaacacaag
     7681 aaccaaaata ctagctcaag gagacaacca ggttttatgt ccgacataca tgttgtcgcc
     7741 agggctatct caagaggggc tcctctatga attggagaga atatcaagga atgcactttc
     7801 gatatacaga gccgtcgagg aaggggcatc taagctaggg ctgatcatca agaaagaaga
     7861 gaccatgtgt agttatgact tcctcatcta tggaaaaacc cctttgttta gaggtaacat
     7921 attggtgcct gagtccaaaa gatgggccag agtctcttgc gtctctaatg accaaatagt
     7981 caacctcgcc aatataatgt cgacagtgtc caccaatgcg ctaacagtgg cacaacactc
     8041 tcaatctttg atcaaaccga tgagggattt tctgctcatg tcagtacagg cagtctttca
     8101 ctacctgcta tttagcccaa tcttaaaggg aagagtttac aagattctga gcgctgaagg
     8161 ggagagcttt ctcctagcca tgtcaaggat aatctatcta gatccttctt tgggagggat
     8221 atctggaatg tccctcggaa gattccatat acgacagttc tcagaccctg tctctgaagg
     8281 gttatccttc tggagagaga tctggttaag ctcccaagag tcctggattc acgcgttgtg
     8341 tcaagaggct ggaaacccag atcttggaga gagaacactc gagagcttca ctcgccttct
     8401 agaagatccg accaccttaa atatcagagg aggggccagt cctaccattc tactcaagga
     8461 tgcaatcaga aaggctttat atgacgaggt ggacaaggtg gaaaattcag agtttcgaga
     8521 ggcaatcctg ttgtccaaga cccatagaga taattttata ctcttcttaa tatctgttga
     8581 gcctctgttt cctcgatttc tcagtgagct attcagttcg tcttttttgg gaatccccga
     8641 gtcaatcatt ggattgatac aaaactcccg aacgataaga aggcagttta gaaagagtct
     8701 ctcaaaaact ttagaagaat ccttctacaa ctcagagatc cacgggatta gtcggatgac
     8761 ccagacacct cagagggttg ggggggtgtg gccttgctct tcagagaggg cagatctact
     8821 tagggagatc tcttggggaa gaaaagtggt aggcacgaca gttcctcacc cttctgagat
     8881 gttgggatta cttcccaagt cctctatttc ttgcacttgt ggagcaacag gaggaggcaa
     8941 tcctagagtt tctgtatcag tactcccgtc ctttgatcag tcattttttt cacgaggccc
     9001 cctaaaggga tacttgggct cgtccacctc tatgtcgacc cagctattcc atgcatggga
     9061 aaaagtcact aatgttcatg tggtgaagag agctctatcg ttaaaagaat ctataaactg
     9121 gttcattact agagattcca acttggctca agctctaatt aggaacatta tgtctctgac
     9181 aggccctgat ttccctctag aggaggcccc tgtcttcaaa aggacggggt cagccttgca
     9241 taggttcaag tctgccagat acagcgaagg agggtattct tctgtctgcc cgaacctcct
     9301 ctctcatatt tctgttagta cagacaccat gtctgatttg acccaagacg ggaagaacta
     9361 cgatttcatg ttccagccat tgatgcttta tgcacagaca tggacatcag agctggtaca
     9421 gagagacaca aggctaagag actctacgtt tcattggcac ctccgatgca acaggtgtgt
     9481 gagacccatt gacgacgtga ccctggagac ctctcagatc ttcgagtttc cggatgtgtc
     9541 gaaaagaata tccagaatgg tttctggggc tgtgcctcac ttccagaggc ttcccgatat
     9601 ccgtctgaga ccaggagatt ttgaatctct aagcggtaga gaaaagtctc accatatcgg
     9661 atcagctcag gggctcttat actcaatctt agtggcaatt cacgactcag gatacaatga
     9721 tggaaccatc ttccctgtca acatatacgg caaggtttcc cctagagact atttgagagg
     9781 gctcgcaagg ggagtattga taggatcctc gatttgcttc ttgacaagaa tgacaaatat
     9841 caatattaat agacctcttg aattggtctc aggggtaatc tcatatattc tcctgaggct
     9901 agataaccat ccctccttgt acataatgct cagagaaccg tctcttagag gagagatatt
     9961 ttctatccct cagaaaatcc ccgccgctta tccaaccact atgaaagaag gcaacagatc
    10021 aatcttgtgt tatctccaac atgtgctacg ctatgagcga gagataatca cggcgtctcc
    10081 agagaatgac tggctatgga tcttttcaga ctttagaagt gccaaaatga cgtacctatc
    10141 cctcattact taccagtctc atcttctact ccagagggtt gagagaaacc tatctaagag
    10201 tatgagagat aacctgcgac aattgagttc tttgatgagg caggtgctgg gcgggcacgg
    10261 agaagatacc ttagagtcag acgacaacat tcaacgactg ctaaaagact ctttacgaag
    10321 gacaagatgg gtggatcaag aggtgcgcca tgcagctaga accatgactg gagattacag
    10381 ccccaacaag aaggtgtccc gtaaggtagg atgttcagaa tgggtctgct ctgctcaaca
    10441 ggttgcagtc tctacctcag caaacccggc ccctgtctcg gagcttgaca taagggccct
    10501 ctctaagagg ttccagaacc ctttgatctc gggcttgaga gtggttcagt gggcaaccgg
    10561 tgctcattat aagcttaagc ctattctaga tgatctcaat gttttcccat ctctctgcct
    10621 tgtagttggg gacgggtcag gggggatatc aagggcagtc ctcaacatgt ttccagatgc
    10681 caagcttgtg ttcaacagtc ttttagaggt gaatgacctg atggcttccg gaacacatcc
    10741 actgcctcct tcagcaatca tgaggggagg aaatgatatc gtctccagag tgatagatct
    10801 tgactcaatc tgggaaaaac cgtccgactt gagaaacttg gcaacctgga aatacttcca
    10861 gtcagtccaa aagcaggtca acatgtccta tgacctcatt atttgcgatg cagaagttac
    10921 tgacattgca tctatcaacc ggatcaccct gttaatgtcc gattttgcat tgtctataga
    10981 tggaccactc tatttggtct tcaaaactta tgggactatg ctagtaaatc caaactacaa
    11041 ggctattcaa cacctgtcaa gagcgttccc ctcggtcaca gggtttatca cccaagtaac
    11101 ttcgtctttt tcatctgagc tctacctccg attctccaaa cgagggaagt ttttcagaga
    11161 tgctgagtac ttgacctctt ccacccttcg agaaatgagc cttgtgttat tcaattgtag
    11221 cagccccaag agtgagatgc agagagctcg ttccttgaac tatcaggatc ttgtgagagg
    11281 atttcctgaa gaaatcatat caaatcctta caatgagatg atcataactc tgattgacag
    11341 tgatgtagaa tcttttctag tccacaagat ggttgatgat cttgagttac agaggggaac
    11401 tctgtctaaa gtggctatca ttatagccat catgatagtt ttctccaaca gagtcttcaa
    11461 cgtttccaaa cccctaactg acccctcgtt ctatccaccg tctgatccca aaatcctgag
    11521 gcacttcaac atatgttgca gtactatgat gtatctatct actgctttag gtgacgtccc
    11581 tagcttcgca agacttcacg acctgtataa cagacctata acttattact tcagaaagca
    11641 agtcattcga gggaacgttt atctatcttg gagttggtcc aacgacacct cagtgttcaa
    11701 aagggtagcc tgtaattcta gcctgagtct gtcatctcac tggatcaggt tgatttacaa
    11761 gatagtgaag actaccagac tcgttggcag catcaaggat ctatccagag aagtggaaag
    11821 acaccttcat aggtacaaca ggtggatcac cctagaggat atcagatcta gatcatccct
    11881 actagactac agttgcctgt gaaccggata ctcctggaag cctgcccatg ctaagactct
    11941 tgtgtgatgt atcttgaaaa aaacaagatc ctaaatctga acctttggtt gtttgattgt
    12001 ttttctcatt tttgttgttt atttgttaag cgtgggtcgg catggcatct ccacctcctc
    12061 gcggtccgac ctgggcatcc gaaggaggac gcacgtccac tcggatggct aagggagggc
    12121 ggggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
    12181 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
    12241 ggaggaacta tatccggatc cactagttct agagcggccg ccaccgcggt ggagctccag
    12301 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
    12361 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    12421 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    12481 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    12541 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    12601 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    12661 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    12721 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    12781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    12841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    12901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    12961 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    13021 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    13081 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    13141 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    13201 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    13261 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    13321 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    13381 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    13441 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    13501 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    13561 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    13621 atctggcccc agtgctgcaa tgataccgcg agacccaccc ttggaggctc cagatttatc
    13681 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    13741 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    13801 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    13861 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    13921 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    13981 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    14041 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    14101 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    14161 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    14221 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    14281 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    14341 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    14401 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    14461 aataggggtt ccgcgcacat ttccccgaaa agtgccacc
//
