LOCUS       sequence-401533-        9661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-FCRL5-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3500..3535
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             3545..3568
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5228..5272
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             5273..5299
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(5439..5456)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5445..5556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5674..5694)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5679..6008
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5859..5994
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6056..6103
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6122..6721
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6122..6141)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6618..6638
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6851..6972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6888..6907)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6942..6961
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(7025..7816,7817..7885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7648..7667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7886..7977)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8019..8038
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8044..8063
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8149..8737
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8638..8657
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8891..8908
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9006..9023
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9007..9023
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9479..9495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg cccaatttgc cagaactccc cggcctatca tcttcctgca
     1021 gcctccatgg accaccgtgt ttcagggcga aagagtgacc ctgacctgca agggcttcag
     1081 attctacagc cctcaaaaga ccaagtggta tcaccgctac ctgggcaaag agatcctgag
     1141 agagacaccc gacaacatcc tggaagtgca agagtccggc gagtacagat gccaggctca
     1201 gggatctcct ctgtctagcc ctgtgcacct ggatttttcc agcgccagcc tgattctgca
     1261 ggcccctctg tctgtgttcg agggcgattc tgtggtgctg agatgcagag ccaaggccga
     1321 agtgacactg aacaacacca tctacaagaa cgacaacgtg ctggccttcc tgaacaagcg
     1381 gaccgacttt cacatccctc acgcctgcct gaaggacaac ggcgcctaca gatgtaccgg
     1441 ctacaaagag agctgctgcc ccgtcagcag caacaccgtg aagattcagg tgcaagagcc
     1501 cttcaccaga cctgtgctga gagccagcag ctttcagccc atcagcggca atcctgtgac
     1561 tctgacatgc gagacacagc tgtccctgga aagatccgac gtgcccctga gattccggtt
     1621 cttcagagat gatcagaccc tcggcctcgg ctggtccctg tctcctaatt ttcagatcac
     1681 cgccatgtgg tccaaggaca gcggctttta ctggtgcaag gccgccacaa tgccctacag
     1741 cgtgatcagc gatagcccca gatcctggat acaggtgcag atccctgcca gccatccagt
     1801 gctgacactg tctccagaga aggccctgaa cttcgagggc accaaagtga ctctgcactg
     1861 tgaaacccaa gaggacagcc tgcggaccct gtacagattc tatcacgagg gcgtgccact
     1921 gcggcacaag tctgttagat gcgagagagg cgccagcatc agctttagcc tgaccaccga
     1981 gaacagcggc aactactact gcaccgccga taatggactg ggcgccaagc cttctaaggc
     2041 cgtgtcactg tctgtgaccg tgcctgtgtc tcaccccgtt ctgaatctga gcagccccga
     2101 ggatctgatt tttgaaggcg ccaaagtcac cctgcattgc gaggctcaaa gaggctccct
     2161 gcctatcctg taccagttcc atcatgaagg cgctgccctc gagagaagaa gcgctaattc
     2221 tgccggcgga gtggccatct cattttctct gacagccgag cactccggga attactactg
     2281 tacagccgac aacggcttcg gcccacagag aagtaaagcc gtgtctctga gcgtgacagt
     2341 gcccgtttct catcctgtgc ttaccctgtc tagcgccgag gctcttacat ttgagggcgc
     2401 cactgttaca ctccactgcg aggttcagag gggctctccc cagattctgt atcagtttta
     2461 ccacgaggac atgcccctgt ggtctagctc tacaccttct gtgggcagag tgtcctttag
     2521 tttcagcctg acagagggcc acagcgggaa ctattattgc acagctgaca atggctttgg
     2581 accccagcgg agcgaggtgg tgtctctgtt tgtgacagtc ccagtgtcca gacctatcct
     2641 gacactgaga gtgcctagag cacaggccgt tgtgggagat ctgctggaac tgcactgcga
     2701 agcccctaga ggaagccctc caatcctgta ttggttctac catgaggacg tgaccctggg
     2761 aagcagctct gctccttctg gcggcgaggc ctcttttaat ctgagcctga ctgccgaaca
     2821 ctctggcaac tatagctgcg aggccaacaa tggcctggtg gcccagcact ctgataccat
     2881 cagtctgtcc gtgattgtgc ccgtgtctcg gcccatcctg acctttagag cacctagggc
     2941 tcaagctgtt gtgggcgacc tgctcgagct tcattgtgaa gccctgagag gctctagccc
     3001 cattctgtac tggttttatc acgaagatgt caccctgggc aagatcagcg ctcctagcgg
     3061 aggcggagct tcctttaatc tgtccctgac cacagagcat tccggcatct actcttgcga
     3121 agccgataac ggcctggaag cccagagaag cgagatggtc acactgaagg tggccgtgcc
     3181 agttagtaga cccgtgctga ctctgagagc acctggaaca catgctgccg ttggcgatct
     3241 cctcgaactc cactgtgaag ctctgcgggg aagcccactg atcctctacc ggttctttca
     3301 cgaggatgtg acactgggga acagaagctc tccaagcggc ggagcaagcc tgaacctgtc
     3361 tctgaccgct gagcatagcg gaaactacag ctgtgaagca gacaacggac tgggtgctca
     3421 gagatccgaa accgtgacac tgtacatcac aggcctgacc gccaacagaa gcggaccttt
     3481 tgctaccggt ggcgcgccgg aagatcaagt tgatccaaga ttgattgatg gcaagggcgg
     3541 atctctggag gtgctgttcc agggaccagg cggaagcggc aatcccggcg gcgatctggc
     3601 cccccagatg ctgcgcgagc tgcaggagac caatgccgcc ctgcaggacg tgcgcgagct
     3661 gctgcgccac cgcgtgaagg agatcacctt cctgaagaac accgtgatgg agtgcgacgc
     3721 ctgcggtatg cagccctcta gaggctccgg ctccatcatc ccagttgagg aggagaaccc
     3781 ggacttctgg aaccgcgagg cagccgaggc cctgggtgcc gccaagaagc tgcagcctgc
     3841 acagacagcc gccaagaacc tcatcatctt cctgggcgat gggatggggg tgtctacggt
     3901 gacagctgcc aggatcctaa aagggcagaa gaaggacaaa ctggggcctg agatacccct
     3961 ggccatggac cgcttcccat atgtggctct gtccaagaca tacaatgtag acaaacatgt
     4021 gccagacagt ggagccacag ccacggccta cctgtgcggg gtcaagggca acttccagac
     4081 cattggcttg agtgcagccg cccgctttaa ccagtgcaac acgacacgcg gcaacgaggt
     4141 catctccgtg atgaatcggg ccaagaaagc agggaagtca gtgggagtgg taaccaccac
     4201 acgagtgcag cacgcctcgc cagccggcac ctacgcccac acggtgaacc gcaactggta
     4261 ctcggacgcc gacgtgcctg cctcggcccg ccaggagggg tgccaggaca tcgctacgca
     4321 gctcatctcc aacatggaca ttgacgtgat cctaggtgga ggccgaaagt acatgtttcg
     4381 catgggaacc ccagaccctg agtacccaga tgactacagc caaggtggga ccaggctgga
     4441 cgggaagaat ctggtgcagg aatggctggc gaagcgccag ggtgcccggt atgtgtggaa
     4501 ccgcactgag ctcatgcagg cttccctgga cccgtctgtg acccatctca tgggtctctt
     4561 tgagcctgga gacatgaaat acgagatcca ccgagactcc acactggacc cctccctgat
     4621 ggagatgaca gaggctgccc tgcgcctgct gagcaggaac ccccgcggct tcttcctctt
     4681 cgtggagggt ggtcgcatcg accatggtca tcatgaaagc agggcttacc gggcactgac
     4741 tgagacgatc atgttcgacg acgccattga gagggcgggc cagctcacca gcgaggagga
     4801 cacgctgagc ctcgtcactg ccgaccactc ccacgtcttc tccttcggag gctaccccct
     4861 gcgagggagc tccatcttcg ggctggcccc tggcaaggcc cgggacagga aggcctacac
     4921 ggtcctccta tacggaaacg gtccaggcta tgtgctcaag gacggcgccc ggccggatgt
     4981 taccgagagc gagagcggga gccccgagta tcggcagcag tcagcagtgc ccctggacga
     5041 agagacccac gcaggcgagg acgtggcggt gttcgcgcgc ggcccgcagg cgcacctggt
     5101 tcacggcgtg caggagcaga ccttcatagc gcacgtcatg gccttcgccg cctgcctgga
     5161 gccctacacc gcctgcgacc tggcgccccc cgccggcacc accgacgccg cgcacccggg
     5221 tggcggagga ctgaacgaca tcttcgaggc tcagaaaatc gaatggcacg aacatcacca
     5281 tcaccatcac catcaccatt aaggccggcc tgtagtcact ggcacaacgc gttacccggt
     5341 aagccaatcg ggtatacacg gtcgtcatac tgcagacagg gttcttctac tttgcaagat
     5401 agtcttgagt agtaaaataa atagatagag aaaatcagcc tcgactgtgc cttctagttg
     5461 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     5521 cactgtcctt tcctaataaa atgaggaaat tgcatcacaa cactcaaccc tatctcggtc
     5581 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5641 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     5701 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5761 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5821 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5881 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5941 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6001 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     6061 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     6121 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6181 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6241 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6301 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6361 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6421 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6481 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6541 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6601 ggagacatcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6661 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6721 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     6781 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     6841 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     6901 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6961 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     7021 gtcattacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7081 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7141 gccccagcgc tgcgatgata ccgcgagaac cacgctcacc ggctccggat ttatcagcaa
     7201 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7261 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7321 gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7381 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7441 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7501 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7561 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7621 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7681 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7741 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7801 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7861 cgacacggaa atgttgaata ctcatattct tcctttttca atattattga agcatttatc
     7921 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7981 gggtcagtgt tacaaccaat taaccaattc tgaacattat cgcgagccca tttatacctg
     8041 aatatggctc ataacacccc ttgctcatga ccaaaatccc ttaacgtgag ttacgcgcgc
     8101 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8161 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8221 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8281 gataccaaat actgttcttc tagtgtagcc gtagttagcc caccacttca agaactctgt
     8341 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8401 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8461 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8521 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8581 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8641 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8701 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8761 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8821 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8881 gaccgagcgc agcgagtcag tgagcgagga agcggaaggc gagagtaggg aactgccagg
     8941 catcaaacta agcagaaggc ccctgacgga tggccttttt gcgtttctac aaactctttc
     9001 tgtgttgtaa aacgacggcc agtcttaagc tcgggccccc tgggcggttc tgataacgag
     9061 taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt atggggggag
     9121 tttagggaaa gagcatttgt cagaatattt aagggcgcct gtcactttgc ttgatatatg
     9181 agaattattt aaccttataa atgagaaaaa agcaacgcac tttaaataag atacgttgct
     9241 ttttcgattg atgaacacct ataattaaac tattcatcta ttatttatga ttttttgtat
     9301 atacaatatt tctagtttgt taaagagaat taagaaaata aatctcgaaa ataataaagg
     9361 gaaaatcagt ttttgatatc aaaattatac atgtcaacga taatacaaaa tataatacaa
     9421 actataagat gttatcagta tttattatca tttagaataa attttgtgtc gcccttaatt
     9481 gtgagcggat aacaattacg agcttcatgc acagtggcgt tgactcaata ttggccatta
     9541 gccatattat tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg
     9601 ttgtatctat atcataatat gtacatttat attggctcat gtccaatatg accgccatgt
     9661 t
//
