LOCUS       sequence-401572-       13974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing human phosphoinositide 3-kinase 
            catalytic subunit alpha (PIK3CA) under the spleen focus-forming 
            virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13974)
  AUTHORS   Nieuwenhuis B, Barber AC, Evans RS, Pearson CS, Fuchs J, MacQueen 
            AR, van Erp S, Haenzi B, Hulshof LA, Osborne A, Conceicao R, Khatib 
            TZ, Deshpande SS, Cave J, Ffrench-Constant C, Smith PD, Okkenhaug K,
            Eickholt BJ, Martin KR, Fawcett JW, Eva R
  TITLE     PI 3-kinase delta enhances axonal PIP3 to support axon regeneration 
            in the adult CNS.
  JOURNAL   EMBO Mol Med. 2020 Aug 7;12(8):e11674. doi: 10.15252/emmm.201911674.
            Epub 2020 Jun 17.
  PUBMED    32558386
REFERENCE   2  (bases 1 to 13974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Mol 
            Med."; date: "2020-08-7"; volume: "12(8):e11674. doi"; pages: " 
            10.15252/emmm.201911674. Epub 2020 Jun 17"
COMMENT     Sequence Label: pLV-SFFV-PIK3CA-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..13974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2320..2727
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(6055..6075)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6473..6484)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7523..7542
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      7716..7725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7722..7724,7725..7727,7728..8441)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7767..7788)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8028..8047)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8375..8396
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             8536..8769
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8794..8812)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8795..8812)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9102..9121)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9335..9354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9454..9476
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9514..9532)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9600..9704
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9705..9773,9774..10565)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9923..9942)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10736..11324
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11225..11244
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11478..11495
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11565..11585)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11570..11899
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11750..11885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          13033..13098
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13228..13248
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13673..13807
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13710..13729)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13764..13783
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattcctg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga
     2281 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca
     2341 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2401 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2461 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2521 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2581 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2641 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2701 gtcctccgac agactgagtc gcccgggggg gatccatgcc tccaagacca tcatcaggtg
     2761 aactgtgggg catccacttg atgcccccaa gaatcctagt agaatgttta ctaccaaatg
     2821 gaatgatagt gactttagaa tgcctccgtg aggctacatt aataaccata aagcatgaac
     2881 tatttaaaga agcaagaaaa taccccctcc atcaacttct tcaagatgaa tcttcttaca
     2941 ttttcgtaag tgttactcaa gaagcagaaa gggaagaatt ttttgatgaa acaagacgac
     3001 tttgtgacct tcggcttttt caaccctttt taaaagtaat tgaaccagta ggcaaccgtg
     3061 aagaaaagat cctcaatcga gaaattggtt ttgctatcgg catgccagtg tgtgaatttg
     3121 atatggttaa agatccagaa gtacaggact tccgaagaaa tgttctgaac gtttgtaaag
     3181 aagctgtgga tcttagggac ctcaattcac ctcatagtag agcaatgtat gtctatcctc
     3241 caaatgtaga atcttcacca gaattgccaa agcacatata taataaatta gataaagggc
     3301 aaataatagt ggtgatctgg gtaatagttt ctccaaataa tgacaagcag aagtatactc
     3361 tgaaaatcaa ccatgactgt gtaccagaac aagtaattgc tgaagcaatc aggaaaaaaa
     3421 ctcgaagtat gttgctatcc tctgaacaac taaaactctg tgttttagaa tatcagggca
     3481 agtatatttt aaaagtgtgt ggatgtgatg aatacttcct agaaaaatat cctctgagtc
     3541 agtataagta tataagaagc tgtataatgc ttgggaggat gcccaatttg atgttgatgg
     3601 ctaaagaaag cctttattct caactgccaa tggactgttt tacaatgcca tcttattcca
     3661 gacgcatttc cacagctaca ccatatatga atggagaaac atctacaaaa tccctttggg
     3721 ttataaatag tgcactcaga ataaaaattc tttgtgcaac ctacgtgaat gtaaatattc
     3781 gagacattga taagatctat gttcgaacag gtatctacca tggaggagaa cccttatgtg
     3841 acaatgtgaa cactcaaaga gtaccttgtt ccaatcccag gtggaatgaa tggctgaatt
     3901 atgatatata cattcctgat cttcctcgtg ctgctcgact ttgcctttcc atttgctctg
     3961 ttaaaggccg aaagggtgct aaagaggaac actgtccatt ggcatgggga aatataaact
     4021 tgtttgatta cacagacact ctagtatctg gaaaaatggc tttgaatctt tggccagtac
     4081 ctcatggatt agaagatttg ctgaacccta ttggtgttac tggatcaaat ccaaataaag
     4141 aaactccatg cttagagttg gagtttgact ggttcagcag tgtggtaaag ttcccagata
     4201 tgtcagtgat tgaagagcat gccaattggt ctgtatcccg agaagcagga tttagctatt
     4261 cccacgcagg actgagtaac agactagcta gagacaatga attaagggaa aatgacaaag
     4321 aacagctcaa agcaatttct acacgagatc ctctctctga aatcactgag caggagaaag
     4381 attttctatg gagtcacaga cactattgtg taactatccc cgaaattcta cccaaattgc
     4441 ttctgtctgt taaatggaat tctagagatg aagtagccca gatgtattgc ttggtaaaag
     4501 attggcctcc aatcaaacct gaacaggcta tggaacttct ggactgtaat tacccagatc
     4561 ctatggttcg aggttttgct gttcggtgct tggaaaaata tttaacagat gacaaacttt
     4621 ctcagtattt aattcagcta gtacaggtcc taaaatatga acaatatttg gataacttgc
     4681 ttgtgagatt tttactgaag aaagcattga ctaatcaaag gattgggcac tttttctttt
     4741 ggcatttaaa atctgagatg cacaataaaa cagttagcca gaggtttggc ctgcttttgg
     4801 agtcctattg tcgtgcatgt gggatgtatt tgaagcacct gaataggcaa gtcgaggcaa
     4861 tggaaaagct cattaactta actgacattc tcaaacagga gaagaaggat gaaacacaaa
     4921 aggtacagat gaagttttta gttgagcaaa tgaggcgacc agatttcatg gatgctctac
     4981 agggctttct gtctcctcta aaccctgctc atcaactagg aaacctcagg cttgaagagt
     5041 gtcgaattat gtcctctgca aaaaggccac tgtggttgaa ttgggagaac ccagacatca
     5101 tgtcagagtt actgtttcag aacaatgaga tcatctttaa aaatggggat gatttacggc
     5161 aagatatgct aacacttcaa attattcgta ttatggaaaa tatctggcaa aatcaaggtc
     5221 ttgatcttcg aatgttacct tatggttgtc tgtcaatcgg tgactgtgtg ggacttattg
     5281 aggtggtgcg aaattctcac actattatgc aaattcagtg caaaggcggc ttgaaaggtg
     5341 cactgcagtt caacagccac acactacatc agtggctcaa agacaagaac aaaggagaaa
     5401 tatatgatgc agccattgac ctgtttacac gttcatgtgc tggatactgt gtagctacct
     5461 tcattttggg aattggagat cgtcacaata gtaacatcat ggtgaaagac gatggacaac
     5521 tgtttcatat agattttgga cactttttgg atcacaagaa gaaaaaattt ggttataaac
     5581 gagaacgtgt gccatttgtt ttgacacagg atttcttaat agtgattagt aaaggagccc
     5641 aagaatgcac aaagacaaga gaatttgaga ggtttcagga gatgtgttac aaggcttatc
     5701 tagctattcg acagcatgcc aatctcttca taaatctttt ctcaatgatg cttggctctg
     5761 gaatgccaga actacaatct tttgatgaca ttgcatacat tcgaaagacc ctagccttag
     5821 ataaaactga gcaagaggct ttggagtatt tcatgaaaca aatgaatgat gcacatcatg
     5881 gtggctggac aacaaaaatg gattggatct tccacacaat taaacagcat gcattgaact
     5941 gagcggccgc gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga
     6001 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6061 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6121 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6181 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6241 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6301 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6361 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     6421 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6481 caatccagcg gaccttcctt cccgcgggcg cccccctcct cacggcgagc gctgccacgt
     6541 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
     6601 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
     6661 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
     6721 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
     6781 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
     6841 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
     6901 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
     6961 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
     7021 aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt
     7081 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
     7141 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
     7201 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
     7261 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
     7321 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
     7381 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     7441 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     7501 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     7561 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     7621 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     7681 ttagacgaag cttggtaccg agctcggatc aattcgccac catggtgagc aagggcgagg
     7741 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     7801 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     7861 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct
     7921 acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     7981 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     8041 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     8101 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     8161 acagccacaa ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca
     8221 agacccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     8281 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     8341 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     8401 ccgccgggat cactctcggc atggacgagc tgtacaagta actcgaggga attaattcga
     8461 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8521 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     8581 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8641 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8701 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8761 tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg
     8821 tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc
     8881 taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt
     8941 cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga
     9001 aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat
     9061 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     9121 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     9181 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     9241 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     9301 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9361 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     9421 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     9481 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     9541 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     9601 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9661 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9721 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9781 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9841 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9901 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     9961 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10021 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10081 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10141 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10201 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10261 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10321 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10381 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10441 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10501 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    10561 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    10621 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10861 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    10921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    10981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    11041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    11101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    11161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    11281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    11401 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    11461 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    11521 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
    11581 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    11641 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    11701 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
    11761 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    11821 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
    11881 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
    11941 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
    12001 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
    12061 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
    12121 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
    12181 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
    12241 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
    12301 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
    12361 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
    12421 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
    12481 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
    12541 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
    12601 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
    12661 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
    12721 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
    12781 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
    12841 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
    12901 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
    12961 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
    13021 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
    13081 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
    13141 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
    13201 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
    13261 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
    13321 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
    13381 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
    13441 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
    13501 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
    13561 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
    13621 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
    13681 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
    13741 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    13801 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    13861 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    13921 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
