LOCUS       sequence-401577-       13892 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing mouse Llgl1 under the spleen 
            focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin 
            C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13892)
  AUTHORS   Nieuwenhuis, Eva Labs
  TITLE     Plasmid DNA for mammalian expression and viral vectors
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 13892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pLV-SFFV-Llgl1-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..13892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     primer_bind     complement(5644..5664)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6062..6073)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7112..7131
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      7305..7314
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7311..7313,7314..7316,7317..8030)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7356..7377)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7617..7636)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7964..7985
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             8125..8358
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8383..8401)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8384..8401)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8691..8710)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8924..8943)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9043..9065
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9103..9121)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9189..9293
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9294..9362,9363..10154)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9512..9531)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10325..10913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10814..10833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11067..11084
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11154..11174)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11159..11488
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11339..11474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12622..12687
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12817..12837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13262..13396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13299..13318)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13353..13372
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(13564..13892,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttggaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tgatgaagtt tcggttccgg cggcagggcg ccgacccgca
     2461 gcgcgagaag cttaagcagg agctcttcgc cttccacaag actgtggagc atggcttccc
     2521 caatcagccc agcgccttgg ccttcgatcc tgagctccgc atcatggcca tcggcaccag
     2581 atctggggcc gtcaagatct atggtgcacc cggagtggaa tttacaggcc tacatcggga
     2641 cgcagccacc gtcacccaga tgcatttcct ccctggtcag ggccgcctcc tgaccctgct
     2701 agatgacagc agcttgcatc tctgggagat catccatcat aatggctgcg cccacctgga
     2761 ggaaggcctc agcttccacc cacccagcag acccagtttt gacaatgcca gtttccctgc
     2821 cagtctaaca cgtgtcactg tggtcctgct cgtagctggc aatacagcag ccctgggaac
     2881 tgagagtggt agcatattct tcctggatgt agccaccctg gcactgctgg aggggcagac
     2941 tctcagccca gatgtggttc tgcgcagtgt gccagatgat taccggtgtg gaaaggcctt
     3001 gggccctgtg gagtcactcc agggacatct gcaagacccc agcaagatcc tcataggcta
     3061 cagtcggggt ttactggtca tctggagcca ggccacacag tctgtggaca acgttttcct
     3121 aggtaaccag cagctggaga gcctgtgttg gggccgtgat ggcagcagca ttatcagctc
     3181 acacagtgat ggcagctatg ccatctggtc cacagacact ggcagccccc caacgctgca
     3241 gcccactgta gtgaccacac cctacggccc cttcccctgc aaggccatca acaagattct
     3301 gtggcggagc tgtgagtcag gagaccactt tatcatcttc agtggtggca tgcctcgagc
     3361 cagctatggt gaccgccact gtgtgagtgt actgcgagca gagacactgg tgaccctgga
     3421 cttcacctct cgtgtcattg acttcttcac ggtgcacagc acacagccag aggatgagtg
     3481 tgacaacccc caggccttag ccgtgcttct ggaggaggag ctggtggtgc tggacctgca
     3541 gacaccaggc tggccagctg tgcccgcccc ttacctggcc ccactgcatt cgtcagctat
     3601 cacctgctct gcccatgttg ccaatgtccc cagcaagctg tgggcccgca ttgtaagtgc
     3661 tggtgagcag cagagcccac agcctgcctc cagtgccttg agttggccca ttaccggggg
     3721 ccggaacttg gcccaggaac cctcgcagcg tgggctgctg ctcactggcc atgaggatgg
     3781 cactgtgcgg ttctgggacg cctctggtgt ggcgctaagg ccactctaca aactgagcac
     3841 agctggcctc ttccagacgg actgtgaaca tgctgacagc ctggcccagg ctgtggagga
     3901 tgactggccg cccttccgca aggtgggctg ctttgatccc tacagtgatg acccccggct
     3961 aggaatccag aaggttgcgc tttgcaagta cacagcccag atggtggtag ctggcactgc
     4021 aggccaggtg ctggtgctgg agctcagtga ggtcccggca gagcatgccg tcagtgtggc
     4081 caacgtggat cttcttcagg atcgcgaggg cttcacgtgg aagggtcacg agcggctgaa
     4141 cccacacacg gggctgctgc cgtggcctgc cggattccag ccccgcatgc tgatacagtg
     4201 cctcccaccc gccgctgtca ctgctgtcac actccatgct gagtggagcc tcgtggcctt
     4261 tggtaccagt catggctttg gcctttttga ctaccagcgc aagagccctg tgctggctag
     4321 gtgcaccctt caccccaatg attctttggc catggagggg ccactgtcac gggtgaagtc
     4381 cctcaagaag tcactgagac agtcattccg gcgaatccgc aagagccgtg tctcaggcaa
     4441 aaaacggact cctgctgcca gtagcaagtt gcaggaggcc aatgcccagc tggccgagca
     4501 gacctgccca cacgacctgg agatgacacc cgtgcagcgc cgcattgagc ctcggtctgc
     4561 tgacgactcg ctctccggtg ttgtacgctg cctctacttt gctgacacgt tccttcgaga
     4621 tgcgacccac cacgggccca ccatgtgggc gggcaccaac tcgggctctg tgttcgccta
     4681 tgcgctggag gttccagcag ccacagcagg cggagagaag cggcctgagc aggcagtgga
     4741 ggcggtgctg ggcaaggagg tgcagctaat gcaccgagca cctgtggtgg ccattgctgt
     4801 gctggatggt cgtggccggc cactgcctga gccctatgag gcttcccggg acctggccca
     4861 ggcgccagac atgcaaggcg gccatgctgt gctcattgca tctgaggaac aattcaaggt
     4921 gttcacacta cccaaggtga gtgctaagac taaattcaag cttacagccc atgaaggctg
     4981 tcgtgtgcgg aaggtagccc tggctacatt tgccagcgtg atgtctgagg actatgccga
     5041 gacctgcctt gcctgcctca ccaacctggg tgatgtccac gtcttctcgg tgcctggcct
     5101 gaggcctcag gtgcactact cctgtatccg gaaggaggac atcagtggca tcgcttcctg
     5161 tgtcttcaca cgtcacggcc agggctttta cttgatttct ccatcggaat ttgagcgctt
     5221 ctcactgagt gctcgcaaca tcacagaacc actatgttct ctggatataa gctggcccca
     5281 aaatgccacc cagcccaggc ttcaagagtc acccaagctg agccaggcta atgggaccag
     5341 agacatcatt ctggccccag agagctgcga aggaagccct agctctgccc acagcaagcg
     5401 agctgatacc atggaaccac ccgaggccgc tctctcgcct gtgtccattg attcagctgc
     5461 tagtggggat accatgctgg acacaacagg ggatgtcacc gtggaatatg tgaaggattt
     5521 tctgggctga agcggccgcg actctagagt cgacctgcag gcatgcaagc ttgatatcaa
     5581 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5641 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5701 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5761 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5821 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5881 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5941 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6001 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6061 ttcggccctc aatccagcgg accttccttc ccgcgggcgc ccccctcctc acggcgagcg
     6121 ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga
     6181 cagcggcccg ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt
     6241 taggacggga cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg
     6301 aggaaaagta gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc
     6361 gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg
     6421 ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg
     6481 gctggccggg gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac
     6541 cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga
     6601 gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg
     6661 tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc
     6721 cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac
     6781 cctgacgtga agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc
     6841 agttatggcg gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt
     6901 gtcgtgacgt cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt
     6961 gtgcggtagg cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg
     7021 aatcgacagg cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg
     7081 tcggttttat gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg
     7141 gggttggcga gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg
     7201 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
     7261 gcttttttgt tagacgaagc ttggtaccga gctcggatca attcgccacc atggtgagca
     7321 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     7381 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     7441 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     7501 ccttgaccta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
     7561 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     7621 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     7681 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     7741 acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     7801 tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     7861 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     7921 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     7981 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ctcgagggaa
     8041 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8101 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     8161 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8221 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8281 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8341 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
     8401 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
     8461 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
     8521 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
     8581 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
     8641 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     8701 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     8761 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     8821 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     8881 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     8941 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     9001 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     9061 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     9121 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     9181 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     9241 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     9301 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     9361 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     9421 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     9481 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     9541 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9601 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9661 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9721 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9781 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9841 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9901 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9961 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    10021 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    10081 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    10141 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    10201 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    10381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    10441 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    10501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    11041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    11101 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
    11161 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    11221 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
    11281 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
    11341 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    11401 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    11461 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
    11521 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
    11581 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
    11641 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
    11701 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
    11761 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
    11821 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
    11881 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
    11941 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
    12001 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
    12061 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
    12121 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
    12181 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
    12241 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
    12301 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
    12361 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
    12421 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
    12481 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
    12541 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
    12601 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
    12661 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
    12721 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
    12781 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
    12841 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
    12901 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
    12961 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
    13021 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
    13081 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
    13141 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
    13201 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
    13261 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    13321 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    13381 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    13441 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    13501 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    13561 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
    13621 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
    13681 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
    13741 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
    13801 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
    13861 gagagctgca tccggagtac ttcaagaact gc
//
