LOCUS       sequence-401579-        5850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting Neurexin 1beta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5850)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Nxn-1 beta Flag/pCMV
FEATURES             Location/Qualifiers
     source          1..5850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1163..1618
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1250..1269)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1460..1481
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        1936..2315
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2316..2519
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2470..2490
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2588..2632
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /translation="MSALLILALVGAAVA"
     CDS             2633..2656
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3858..4480
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4057..4076)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4504..4524)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4522..4819
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(4827..4846)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4828..4846)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4860..4876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4860..4876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4873..4895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4884..4900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4908..4914,4915..4932,4933..4938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4953..4974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5091..5108)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5262..5850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5342..5361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     1201 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     1261 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     1321 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     1381 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     1441 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     1501 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     1561 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctgatctat acattgaatc
     1801 aatattggca attagccata ttagtcattg gttatatagc ataaatcaat attggctatt
     1861 ggccattgca tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa
     1921 tatgaccgcc atgttgacat tgattattga ctagttatta atagtaatca attacggggt
     1981 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     2041 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     2101 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     2161 acttggcagt acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg
     2221 gtaaatggcc cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc
     2281 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca
     2341 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     2401 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg
     2461 ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
     2521 gtttagtgaa ccgtcagaat tcagatctga attcgatcgt ttagtgaacc gtcagaatta
     2581 attcaccatg tctgcacttc tgatcctagc tcttgttgga gctgcagttg ctgactacaa
     2641 agacgatgac gacaagcttg cggccgcggc atccagtttg ggagcgcacc acatccacca
     2701 tttccatggc agcagcaagc atcattcagt gcctattgca atctacaggt caccagcatc
     2761 cttgcgaggc ggacacgctg ggacgacgta tatctttagc aaaggtggtg gacaaattac
     2821 atataagtgg cctcctaatg accgacccag tacacgagca gacaggctgg ccatcgggtt
     2881 tagcactgtt cagaaggaag cagtgttggt gcgtgtggac agttcctcgg gcctgggtga
     2941 ctaccttgag ctgcacatac accaaggaaa aattggagtt aaatttaatg ttgggacaga
     3001 tgacatcgcc attgaggagt ccaatgcaat cattaatgat ggaaaatacc atgtagtgcg
     3061 tttcacgagg agtggtggca atgccacgtt acaggtggac agctggccag tcatcgaacg
     3121 ataccctgca gggcgtcagc tcacaatctt caatagccaa gcaaccataa taattggcgg
     3181 gaaagagcag ggccagccct tccagggcca gctctctggg ctttactaca atggcttgaa
     3241 agttctgaat atggcggcag agaacgatgc caacatcgcc atagtgggaa atgtgaggct
     3301 ggtcggtgaa gtgccttcct ctatgacaac tgagtcgaca gccactgcca tgcagtccga
     3361 gatgtccacc tcaatcatgg agaccaccac aaccctggct accagcacag ctcgacgagg
     3421 aaagcccccc acaaaggagc ctatcagcca gaccacggat gatatccttg tggcctcggc
     3481 agagtgtccc agcgacgatg aggacattga cccctgtgag ccgagctcag ccaaccccac
     3541 ccgagtgggc ggccgcgaac catacccagg ctcggcagag gtgatccggg agtctagcag
     3601 taccactggc atggtggtgg ggattgtcgc agcagcagct ctgtgcatcc tcatcctcct
     3661 ctatgccatg tacaagtaca gaaaccggga tgaaggttca tatcacgtgg atgagagtcg
     3721 aaactacatc agtaactcag cacagtccaa tggggctgtg gtcaaggaga agcagcccag
     3781 cagtgcgaaa agcgccaaca aaaacaagaa gaacaaggat aaggagtatt atgtctgact
     3841 cgactctaga ggatcccggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
     3901 tggaagttgc cactccagtg cccacgagcc ttgtcctaat aaaattaagt tgcatcattt
     3961 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
     4021 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
     4081 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
     4141 ctcagcctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
     4201 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
     4261 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
     4321 ccttccctgt ccttctgatt ttaaaataac tataccagca ggaggacgtc cagacacagc
     4381 ataggctacc tggccatgcc caaccggtgg gacatttgag ttgcttgctt ggcactgtcc
     4441 tctcatgcgt tgggtccact cagtagatgc ctgttgaatt gggtacgcgg ccagcttggc
     4501 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     4561 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     4621 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     4681 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4741 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4801 agtgaggagg cttttttgga ggaattccct atagtgagtc gtattaaatt cgtaatcatg
     4861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     5041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     5101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     5161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     5221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     5281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     5341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5701 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5821 cagattacgc gcagaaaaaa aggatctcaa
//
