LOCUS       sequence-401585-       11384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human RAB10 under the spleen 
            focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin 
            C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Nieuwenhuis, Eva Labs
  TITLE     Plasmid DNA for mammalian expression and viral vectors
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 11384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pLV-SFFV-RAB10-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..11384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    352..477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    974..1207
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1392..1436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1721..1838
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1991..2398
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2414..2423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3136..3156)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3554..3565)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4604..4623
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4797..4806
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4803..4805,4806..4808,4809..5522)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4848..4869)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5109..5128)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5456..5477
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5617..5850
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5875..5893)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5876..5893)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6183..6202)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6416..6435)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6535..6557
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6595..6613)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6681..6785
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6786..6854,6855..7646)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7004..7023)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7817..8405
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8306..8325
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8559..8576
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8646..8666)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8651..8980
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8831..8966
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10114..10179
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10309..10329
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10754..10888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10791..10810)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10845..10864
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11056..11384,1..305)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
       61 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      301 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      361 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      421 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      481 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      541 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      601 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      661 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      721 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      781 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
      841 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
      901 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
      961 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1021 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1081 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1141 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1201 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1261 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1321 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1381 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1441 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1501 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1561 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1621 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1681 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     1741 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     1801 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     1861 gagatccagt ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa
     1921 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
     1981 gctagctgca gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa
     2041 gttcagatca agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg
     2101 gtgagcagtt tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc
     2161 ggcccgaggc caagaacaga tggtccccag atatggccca accctcagca gtttcttaag
     2221 acccatcaga tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa
     2281 ttaaccaatc agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa
     2341 aagagctcac aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg
     2401 ggatccaccg gtcgccacca tggcgaagaa gacgtacgac ctgcttttca agctgctcct
     2461 gatcggggat tccggagtgg ggaagacctg cgtccttttt cgtttttcgg atgatgcctt
     2521 caatactacc tttatttcca ccataggaat agacttcaag atcaaaacag ttgaattaca
     2581 aggaaagaag atcaagctac agatatggga tacagcaggc caggagcgat ttcacaccat
     2641 cacaacctcc tactacagag gcgcaatggg tatcatgcta gtatatgaca tcaccaatgg
     2701 taaaagtttt gaaaacatca gcaaatggct tagaaacata gatgagcatg ccaatgaaga
     2761 tgtggaaaga atgttactag gaaacaagtg tgatatggac gacaaaagag ttgtacctaa
     2821 aggaaaagga gaacagattg caagggagca tggtattagg ttttttgaga ctagtgcaaa
     2881 agcaaatata aacatcgaaa aggcgttcct cacgttagct gaagatatcc ttcgaaagac
     2941 ccctgtaaaa gagcccaaca gtgaaaatgt agatatcagc agtggaggag gcgtgacagg
     3001 ctggaagagc aaatgctgct gaagcggccg cgactctaga gtcgacctgc aggcatgcaa
     3061 gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     3121 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3181 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3241 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     3301 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3361 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3421 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     3481 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     3541 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcgggc gcccccctcc
     3601 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
     3661 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
     3721 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
     3781 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
     3841 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
     3901 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
     3961 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
     4021 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
     4081 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     4141 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     4201 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
     4261 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     4321 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     4381 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     4441 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     4501 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     4561 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     4621 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     4681 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     4741 tggccgtttt tggctttttt gttagacgaa gcttggtacc gagctcggat caattcgcca
     4801 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4861 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4921 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4981 ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc ccgctacccc gaccacatga
     5041 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5101 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5161 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5221 acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac aagcagaaga
     5281 acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc gtgcagctcg
     5341 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5401 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5461 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5521 aactcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg
     5581 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5641 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     5701 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5761 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     5821 ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata
     5881 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
     5941 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
     6001 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
     6061 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
     6121 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     6181 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     6241 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     6301 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     6361 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa
     6421 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     6481 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     6541 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     6601 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     6661 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6721 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6781 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     6841 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     6901 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     6961 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     7021 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     7081 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     7141 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     7201 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     7261 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     7321 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     7381 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     7441 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     7501 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     7561 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     7621 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     7681 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     7741 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7801 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7861 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7921 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     7981 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8041 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8101 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8161 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8221 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8281 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8341 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8401 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8461 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8521 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8581 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     8641 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     8701 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     8761 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     8821 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8881 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8941 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
     9001 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
     9061 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
     9121 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
     9181 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
     9241 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
     9301 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     9361 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
     9421 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
     9481 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     9541 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
     9601 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     9661 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
     9721 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     9781 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     9841 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     9901 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     9961 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
    10021 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
    10081 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
    10141 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
    10201 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
    10261 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
    10321 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
    10381 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
    10441 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
    10501 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
    10561 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
    10621 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
    10681 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
    10741 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10801 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10861 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    10921 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
    10981 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
    11041 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
    11101 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
    11161 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
    11221 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
    11281 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
    11341 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgc
//
