LOCUS       sequence-401586-        9988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For transfection and in vitro assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Margvelani G, Welden JR, Maquera AA, Van Eyk JE, Murray C, Miranda 
            Sardon SC, Stamm S
  TITLE     Influence of FTDP-17 mutants on circular tau RNAs.
  JOURNAL   Biochim Biophys Acta Mol Basis Dis. 2024 Apr;1870(4):167036. doi: 
            10.1016/j.bbadis.2024.167036. Epub 2024 Jan 28.
  PUBMED    38286213
REFERENCE   2  (bases 1 to 9988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta Mol Basis Dis."; date: "2024-04"; volume: 
            "1870(4):167036. doi"; pages: " 10.1016/j.bbadis.2024.167036. Epub 
            2024 Jan 28"
COMMENT     Sequence Label: Tau BA 12->10 S305N
FEATURES             Location/Qualifiers
     source          1..9988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        195..405
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        217..413
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      264..399
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             480..1274
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     1144..1163
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1448..1569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1618..1634)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1618..1634)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1631..1653)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1642..1658
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1666..1672,1673..1690,1691..1696))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1711..1732
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1849..1866)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2020..2608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2100..2119)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2779..3570,3571..3639))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3402..3421
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3640..3744)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3819..3838)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4010..4389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4390..4593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4544..4564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4638..4657
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4638..4656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6077..6142
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7024..7035
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(7417..7434)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7423..7647
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7693..8121
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7780..7799)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8135..8465
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8316..8451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     gap             8937..8949
                     /estimated_length=13
     gap             8956..9146
                     /estimated_length=191
     misc_feature    9177..9765
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9230..9250)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9648..9659)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9768..9784)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9769..9785)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    9814..9935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
       61 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
      121 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
      181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      241 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      301 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
      361 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
      421 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
      481 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
      541 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
      601 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
      661 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
      721 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
      781 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
      841 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
      901 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
      961 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     1021 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     1081 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1141 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1201 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     1261 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     1321 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     1381 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     1441 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     1501 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     1561 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     1621 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     1681 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     1741 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1801 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1861 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1921 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1981 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2041 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2101 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2161 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2221 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2281 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2341 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2401 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2461 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2521 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2581 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2641 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2701 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2761 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2821 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2881 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2941 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3001 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3061 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3121 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3181 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3241 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3301 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3361 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3421 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3481 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3541 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3601 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3661 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3721 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     3781 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     3841 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     3901 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     3961 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
     4021 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     4081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     4141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     4201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     4261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     4321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     4381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     4441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     4501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     4561 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
     4621 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta
     4681 aacttaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat tcccaggctg
     4741 atggggcctc cagggcagtg gtttccaccc agcattctca aaagagccac tgaggtctca
     4801 gtgccatttt caagatttcg gaagcggcct gggcacggct ggtccttcac tgggatcacc
     4861 acttggcaat tatttacacc tgagacgaat aaaaaccaga gtgctgagat tacaggcatg
     4921 gtggcttacg cttgtaatcg gctttgggaa gccgaggtgg gctgattgct tgagcccagg
     4981 agtttcaaac tatcctggac aacatagcat gacctcgtct ctacaaaaaa tacaaaaaat
     5041 ttgccaggtg tggtggcatg tgcctgtggt cccagctact tgggaggctg aagtaggaga
     5101 atcccctgag ccctgggaag tcgaggctgc actgagccgt gatgatgtca ctgcactcca
     5161 gcctgggtga caaagtgaga ccctatctca caaagaaaaa aaacaaaaca aaaaacccaa
     5221 agcacactgt ttccactgtt tccagagttc ctgagaggaa aggtcaccgg gtgaggaaga
     5281 cgttctcact gatctggcag agaaaatgtc cagtttttcc aactccctaa accatggttt
     5341 tctatttcac agccccccct tgcagggaca gagccctcgt acagaaggga caccccacat
     5401 ttgtcttccc cacaaagcgg cctgtgtcct gcctacgggg tcagggcttc tcaaacctgg
     5461 ctgtgtgtca gaatcaccag gggaactttt caaaactaga gagactgaag ccagactcct
     5521 agattctaat tctaggtcag ggctaggggc tgagattgta aaaatccaca ggtgattctg
     5581 atgcccggca ggcttgagaa cagccgcagg gagttctctg ggaatgtgcc ggtgggtcta
     5641 gccaggtgtg agtggagatg ccggggaact tcctattact cactcgtcag tgtggccgaa
     5701 cacatttttc acttgacctc aggctggtga acgctcccct ctggggttca ggcctcacga
     5761 tgccatcctt ttgtgaagtg aggacctgca atcccagctt cgtaaagccc gctggaaatc
     5821 actcacactt ctgggatgcc ttcagagcag ccctctatcc cttcagctcc cctgggatgt
     5881 gactcgacct cccgtcactc cccagactgc ctctgccaag tccgaaagtg gaggcatcct
     5941 tgcgagcaag taggcgggtc cagggtggcg catgtcactc atcgaaagtg gaggcgtcct
     6001 tgcgagcaag caggcgggtc cagggtggcg tgtcactcat ccttttttct ggctaccaaa
     6061 ggtgcagata attaatgact acaaagacca tgacggtgat tataaagatc atgacatcga
     6121 ttacaaggat gacgatgaca agaagaagct ggatcttagc aacgtccagt ccaagtgtgg
     6181 ctcaaaggat aatatcaaac acgtcccggg aggcggcaat gtgcaaatag tctacaaacc
     6241 agttgacctg agcaaggtga cctccaagtg tggctcatta ggcaacatcc atcataaacc
     6301 aggaggtggc caggtggaag taaaatctga gaagcttgac ttcaaggaca gagtccagtc
     6361 gaagattggg tccctggaca atatcaccca cgtccctggc ggaggaaata aaaaggtaaa
     6421 gggggtaggg tgggttggat gctgcccttg ggtatatggg cattaatcaa gttgagtgga
     6481 caaaggctgg tccagttccc agaggaggaa aacagaggct tctgtgttga ctggctggat
     6541 gtgggccctc agcagcatcc agtgggtctc cactgcctgt ctcaatcacc tggagcttta
     6601 gcacgtttca cacctgggcc ccaacctgga gaggctgacc aatgggtctc aggggcagct
     6661 cggttgctgg agtttttgtt tttatttatt tttatgtatt taaggcaggg tctctgtatt
     6721 agtccattct cacactgcta ataaagacat acccaagact gggtaattta taaaggaaag
     6781 aggtttaatg gactcacagt tccatatggc tggggaggcc tcaaaatcat ggcggaaggc
     6841 aaaggagaag caaaggcatg tcttacatgg caacaggcaa gagagcgtgt gcaggggaac
     6901 tcccatttat aaaaccatca gacctcatga gatttattca ctatcatgag aacagcatgg
     6961 gaaagacccg cccccatgat tcagttacct cccactgggt ccctcccatg acacatggaa
     7021 ttatgggagc tacaattcaa gatgagattt gggtggggac acagccaaac catatcagtc
     7081 tccctctgtc atccaggctg gagtgcactg gcatgatctc ggctcactgc agcctctacc
     7141 tccctgggtc aggtgatctt cccacctcag cctcccaggt agctggaact acaggtacct
     7201 gccactatgc ctggctaaat attttgtatt tcctgtggag acgaggtttt gccacgttgc
     7261 ccaggctggt cttgaactcc tgaggtcaag caatatgccc acctcggcct cccaaggtgc
     7321 tgggattaca ggtgtgagcc acagtgctcg gcctaagtca ctgcagtttt tagcggccgc
     7381 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     7441 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     7501 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     7561 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     7621 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     7681 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     7741 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     7801 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     7861 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     7921 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     7981 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     8041 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     8101 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     8161 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     8221 gtgtnggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8281 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8341 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8401 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     8461 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     8521 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     8581 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     8641 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     8701 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     8761 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     8821 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     8881 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccacnnnn
     8941 nnnnnnnnnr catcgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnstnt ayragtwmra dwwmnngctt atcgataatc
     9181 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     9241 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     9301 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     9361 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     9421 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     9481 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     9541 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     9601 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     9661 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     9721 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     9781 ctcgacccgg gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     9841 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     9901 tatttgtaac cattataagc tgcaataaac aagttrrsrw srrcrawkrm mtycakywtr
     9961 wgwtycmsgy kcwggrggak mwkysrgm
//
