LOCUS       sequence-401592-       11384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human RAB10 mutation Q68L 
            (constitutively active mutant) under the spleen focus-forming virus 
            (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Nieuwenhuis, Eva Labs
  TITLE     Plasmid DNA for mammalian expression and viral vectors
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 11384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pLV-SFFV-RAB10 Q68L-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..11384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2050..2167
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2320..2727
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2743..2752
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3465..3485)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3883..3894)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4933..4952
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5126..5135
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5132..5134,5135..5137,5138..5851)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5177..5198)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5438..5457)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5785..5806
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5946..6179
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6204..6222)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6205..6222)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6512..6531)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6745..6764)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6864..6886
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6924..6942)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7010..7114
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7115..7183,7184..7975)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7333..7352)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8146..8734
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8635..8654
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8888..8905
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8975..8995)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8980..9309
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9160..9295
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10443..10508
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10638..10658
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11083..11217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11120..11139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11174..11193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattcctg cagccccgat aaaataaaag attttattta gtctccagaa aaagggggga
     2281 atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt ttgcaaggca
     2341 tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac atgaaaatag
     2401 ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc ccggggccaa
     2461 gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat ggtccccaga
     2521 tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc tcccccaagg
     2581 acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct cgcttctgtt
     2641 cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact cggcgcgcca
     2701 gtcctccgac agactgagtc gcccgggggg gatccaccgg tcgccaccat ggcgaagaag
     2761 acgtacgacc tgcttttcaa gctgctcctg atcggggatt ccggagtggg gaagacctgc
     2821 gtcctttttc gtttttcgga tgatgccttc aatactacct ttatttccac cataggaata
     2881 gacttcaaga tcaaaacagt tgaattacaa ggaaagaaga tcaagctaca gatatgggat
     2941 acagcaggcc tggagcgatt tcacaccatc acaacctcct actacagagg cgcaatgggt
     3001 atcatgctag tatatgacat caccaatggt aaaagttttg aaaacatcag caaatggctt
     3061 agaaacatag atgagcatgc caatgaagat gtggaaagaa tgttactagg aaacaagtgt
     3121 gatatggacg acaaaagagt tgtacctaaa ggaaaaggag aacagattgc aagggagcat
     3181 ggtattaggt tttttgagac tagtgcaaaa gcaaatataa acatcgaaaa ggcgttcctc
     3241 acgttagctg aagatatcct tcgaaagacc cctgtaaaag agcccaacag tgaaaatgta
     3301 gatatcagca gtggaggagg cgtgacaggc tggaagagca aatgctgctg aagcggccgc
     3361 gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga taatcaacct
     3421 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3481 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3541 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3601 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3661 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3721 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3781 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     3841 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3901 gaccttcctt cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
     3961 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
     4021 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
     4081 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
     4141 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
     4201 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
     4261 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
     4321 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
     4381 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
     4441 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
     4501 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
     4561 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
     4621 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
     4681 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
     4741 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     4801 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
     4861 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
     4921 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
     4981 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
     5041 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacgaag
     5101 cttggtaccg agctcggatc aattcgccac catggtgagc aagggcgagg agctgttcac
     5161 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     5221 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     5281 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttgacct acggcgtgca
     5341 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     5401 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     5461 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     5521 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     5581 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca
     5641 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     5701 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     5761 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     5821 cactctcggc atggacgagc tgtacaagta actcgaggga attaattcga gctcggtacc
     5881 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5941 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     6001 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6061 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6121 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6181 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     6241 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     6301 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     6361 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     6421 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     6481 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     6541 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     6601 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     6661 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     6721 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     6781 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     6841 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     6901 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     6961 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7021 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7081 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7141 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7201 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7261 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7321 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     7381 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7441 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7501 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7561 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7621 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7681 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7741 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7801 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7861 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7921 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7981 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8041 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8101 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8161 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8221 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8281 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8341 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8401 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8461 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8521 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8581 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8641 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8701 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8761 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8821 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8881 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     8941 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
     9001 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9061 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     9121 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9181 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9241 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9301 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     9361 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     9421 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     9481 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     9541 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     9601 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
     9661 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     9721 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
     9781 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
     9841 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
     9901 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
     9961 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
    10021 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
    10081 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
    10141 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
    10201 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
    10261 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
    10321 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
    10381 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
    10441 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
    10501 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
    10561 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
    10621 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
    10681 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
    10741 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
    10801 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
    10861 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
    10921 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
    10981 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
    11041 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
    11101 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    11161 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    11221 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    11281 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    11341 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
