LOCUS       sequence-401645-       11384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector plasmid expressing human RAB10 mutation T23N 
            (dominant negative mutant) under the spleen focus-forming virus 
            (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11384)
  AUTHORS   Nieuwenhuis, Eva Labs
  TITLE     Plasmid DNA for mammalian expression and viral vectors
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 11384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald
FEATURES             Location/Qualifiers
     source          1..11384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    305..430
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    927..1160
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1345..1389
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1674..1791
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1944..2351
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2367..2376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3089..3109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3507..3518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4557..4576
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4750..4759
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4756..4758,4759..4761,4762..5475)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4801..4822)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5062..5081)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5409..5430
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5570..5803
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5828..5846)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5829..5846)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6136..6155)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6369..6388)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6488..6510
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6548..6566)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6634..6738
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6739..6807,6808..7599)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6957..6976)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7770..8358
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8259..8278
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8512..8529
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8599..8619)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8604..8933
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8784..8919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10067..10132
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10262..10282
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10707..10841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10744..10763)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10798..10817
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(11009..11384,1..258)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc
       61 tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      121 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      181 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      241 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      301 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      361 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      421 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      481 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
      541 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
      601 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
      661 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
      721 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg
      781 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
      841 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
      901 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
      961 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     1021 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     1081 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     1141 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     1201 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     1261 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     1321 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     1381 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     1441 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     1501 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     1561 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     1621 cgattagtga acggatctcg acggtatcgc caaatggcag tattcatcca caattttaaa
     1681 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     1741 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     1801 tacagggaca gcagagatcc agtttggatc gataagcttg atatcgaatt cctgcagccc
     1861 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     1921 gtaggtttgg caagctagct gcagtaacgc cattttgcaa ggcatggaaa aataccaaac
     1981 caagaataga gaagttcaga tcaagggcgg gtacatgaaa atagctaacg ttgggccaaa
     2041 caggatatct gcggtgagca gtttcggccc cggcccgggg ccaagaacag atggtcaccg
     2101 cagtttcggc cccggcccga ggccaagaac agatggtccc cagatatggc ccaaccctca
     2161 gcagtttctt aagacccatc agatgtttcc aggctccccc aaggacctga aatgaccctg
     2221 cgccttattt gaattaacca atcagcctgc ttctcgcttc tgttcgcgcg cttctgcttc
     2281 ccgagctcta taaaagagct cacaacccct cactcggcgc gccagtcctc cgacagactg
     2341 agtcgcccgg gggggatcca ccggtcgcca ccatggcgaa gaagacgtac gacctgcttt
     2401 tcaagctgct cctgatcggg gattccggag tggggaagaa ctgcgtcctt tttcgttttt
     2461 cggatgatgc cttcaatact acctttattt ccaccatagg aatagacttc aagatcaaaa
     2521 cagttgaatt acaaggaaag aagatcaagc tacagatatg ggatacagca ggccaggagc
     2581 gatttcacac catcacaacc tcctactaca gaggcgcaat gggtatcatg ctagtatatg
     2641 acatcaccaa tggtaaaagt tttgaaaaca tcagcaaatg gcttagaaac atagatgagc
     2701 atgccaatga agatgtggaa agaatgttac taggaaacaa gtgtgatatg gacgacaaaa
     2761 gagttgtacc taaaggaaaa ggagaacaga ttgcaaggga gcatggtatt aggttttttg
     2821 agactagtgc aaaagcaaat ataaacatcg aaaaggcgtt cctcacgtta gctgaagata
     2881 tccttcgaaa gacccctgta aaagagccca acagtgaaaa tgtagatatc agcagtggag
     2941 gaggcgtgac aggctggaag agcaaatgct gctgaagcgg ccgcgactct agagtcgacc
     3001 tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt
     3061 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3121 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3181 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3241 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3301 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3361 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3421 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     3481 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3541 ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct
     3601 gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc ggccttagaa
     3661 ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag ggcactggtt
     3721 ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga ttctgcggag
     3781 ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg tgtggcacag
     3841 ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt
     3901 cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc gggccgctcg
     3961 gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg cgagcaaggt
     4021 tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa
     4081 tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg
     4141 cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta ttcgggtgag
     4201 atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact ggagaactcg
     4261 gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt gcacccgtac
     4321 ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc
     4381 agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg
     4441 ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt gaggggaggg
     4501 ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta agtagctgaa
     4561 gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc
     4621 accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt
     4681 gtccgctaaa ttctggccgt ttttggcttt tttgttagac gaagcttggt accgagctcg
     4741 gatcaattcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4801 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4861 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4921 gtgccctggc ccaccctcgt gaccaccttg acctacggcg tgcagtgctt cgcccgctac
     4981 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     5041 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     5101 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     5161 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaaggtcta tatcaccgcc
     5221 gacaagcaga agaacggcat caaggtgaac ttcaagaccc gccacaacat cgaggacggc
     5281 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5341 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     5401 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5461 gagctgtaca agtaactcga gggaattaat tcgagctcgg tacctttaag accaatgact
     5521 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5581 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5641 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5701 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5761 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc
     5821 cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat
     5881 tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga
     5941 agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct
     6001 tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg
     6061 tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
     6121 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
     6181 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
     6241 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
     6301 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc
     6361 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     6421 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     6481 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     6541 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     6601 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6661 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6721 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     6781 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     6841 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     6901 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     6961 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7021 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7081 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7141 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7201 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7261 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7321 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7381 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7441 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7501 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7561 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     7621 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     7681 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     7741 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     7801 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     7861 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7921 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7981 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8041 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8101 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8161 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8221 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8281 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8341 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8401 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8461 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8521 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     8581 attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8641 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     8701 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8761 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8821 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     8881 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
     8941 gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga
     9001 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
     9061 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
     9121 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
     9181 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
     9241 cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata
     9301 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
     9361 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
     9421 cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
     9481 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac
     9541 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
     9601 gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta
     9661 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
     9721 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
     9781 cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
     9841 ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt
     9901 gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt
     9961 ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg
    10021 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
    10081 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
    10141 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
    10201 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
    10261 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
    10321 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
    10381 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
    10441 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
    10501 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
    10561 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
    10621 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    10681 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    10741 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    10801 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    10861 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    10921 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    10981 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
    11041 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
    11101 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
    11161 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
    11221 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
    11281 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
    11341 tatcgagctt gctacaaggg actttccgct ggggactttc cagg
//
