LOCUS       sequence-401667-       10040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10040)
  AUTHORS   Tee AR, Fingar DC, Manning BD, Kwiatkowski DJ, Cantley LC, Blenis J
  TITLE     Tuberous sclerosis complex-1 and -2 gene products function together 
            to inhibit mammalian target of rapamycin (mTOR)-mediated downstream 
            signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Oct 15. 99(21):13571-6.
  PUBMED    12271141
REFERENCE   2  (bases 1 to 10040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Oct 15. 99(21):13571-6."
COMMENT     Sequence Label: pRK7-FLAG-TSC2
FEATURES             Location/Qualifiers
     source          1..10040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        435..453
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     435..452
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     466..485
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             540..563
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      5947..5956
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5960..6094
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5997..6016)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6051..6070
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        6163..6520
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6163..6354
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6186..6206)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6371..6506
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6540..6556)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6549..6571)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6769..7224
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6856..6875)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7066..7087
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7241..7260)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7360..7382
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7420..7438)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7506..7610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7611..7679,7680..8471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7829..7848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8642..9230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9131..9150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9384..9401
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9518..9539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9554..9559,9560..9577,9578..9584)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9592..9608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9597..9619
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9616..9632
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9616..9632
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        9661..10040
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccgggaa
      301 cggtgcattg gaacgcggat tccccgtgcc aagagtgacg taagtaccgc ctatagagtc
      361 tataggccca cccccttggc ttcgttagaa cgcggctaca attaatacat aaccttatgt
      421 atcatacaca tacgatttag gtgacactat agaataacat ccactttgcc tttctctcca
      481 caggtgtcca ctcccaggtc caactgcacc tcggttctaa gcttctgcag gtcgacatgg
      541 actataagga cgatgatgac aaagccaaac caacaagcaa agattcaggc ttgaaggaga
      601 agtttaagat tctgttggga ctgggaacac cgaggccaaa tcccaggtct gcagagggta
      661 aacagacgga gtttatcatc accgcggaaa tactgagaga actgagcatg gaatgtggcc
      721 tcaacaatcg catccggatg atagggcaga tttgtgaagt cgcaaaaacc aagaaatttg
      781 aagagcacgc agtggaagca ctctggaagg cggtcgcgga tctgttgcag ccggagcggc
      841 cgctggaggc ccggcacgcg gtgctggctc tgctgaaggc catcgtgcag gggcagggcg
      901 agcgtttggg ggtcctcaga gccctcttct ttaaggtcat caaggattac ccttccaacg
      961 aagaccttca cgaaaggctg gaggttttca aggccctcac agacaatggg agacacatca
     1021 cctacttgga ggaagagctg gctgactttg tcctgcagtg gatggatgtt ggcttgtcct
     1081 cggaattcct tctggtgctg gtgaacttgg tcaaattcaa tagctgttac ctcgacgagt
     1141 acatcgcaag gatggttcag atgatctgtc tgctgtgcgt ccggaccgcg tcctctgtgg
     1201 acatagaggt ctccctgcag gtgctggacg ccgtggtctg ctacaactgc ctgccggctg
     1261 agagcctccc gctgttcatc gttaccctct gtcgcaccat caacgtcaag gagctctgcg
     1321 agccttgctg gaagctgatg cggaacctcc ttggcaccca cctgggccac agcgccatct
     1381 acaacatgtg ccacctcatg gaggacagag cctacatgga ggacgcgccc ctgctgagag
     1441 gagccgtgtt ttttgtgggc atggctctct ggggagccca ccggctctat tctctcagga
     1501 actcgccgac atctgtgttg ccatcatttt accaggccat ggcatgtccg aacgaggtgg
     1561 tgtcctatga gatcgtcctg tccatcacca ggctcatcaa gaagtatagg aaggagctcc
     1621 aggtggtggc gtgggacatt ctgctgaaca tcatcgaacg gctccttcag cagctccaga
     1681 ccttggacag cccggagctc aggaccatcg tccatgacct gttgaccacg gtggaggagc
     1741 tgtgtgacca gaacgagttc cacgggtctc aggagagata ctttgaactg gtggagagat
     1801 gtgcggacca gaggcctgag tcctccctcc tgaacctgat ctcctataga gcgcagtcca
     1861 tccacccggc caaggacggc tggattcaga acctgcaggc gctgatggag agattcttca
     1921 ggagcgagtc ccgaggcgcc gtgcgcatca aggtgctgga cgtgctgtcc tttgtgctgc
     1981 tcatcaacag gcagttctat gaggaggagc tgattaactc agtggtcatc tcgcagctct
     2041 cccacatccc cgaggataaa gaccaccagg tccgaaagct ggccacccag ttgctggtgg
     2101 acctggcaga gggctgccac acacaccact tcaacagcct gctggacatc atcgagaagg
     2161 tgatggcccg ctccctctcc ccacccccgg agctggaaga aagggatgtg gccgcatact
     2221 cggcctcctt ggaggatgtg aagacagccg tcctggggct tctggtcatc cttcagacca
     2281 agttgtacac cctgcctgca agccacgcca cgcgtgtgta tgagatgctg gtcagccaca
     2341 ttcagctcca ctacaagcac agctacaccc tgccaatcgc gagcagcatc cggctgcagg
     2401 cctttgactt cctgttgctg ctgcgggccg actcactgca ccgcctgggc ctgcccaaca
     2461 aggatggagt cgtgcggttc agcccctact gcgtctgcga ctacatggag ccagagagag
     2521 gctctgagaa gaagaccagc ggcccccttt ctcctcccac agggcctcct ggcccggcgc
     2581 ctgcaggccc cgccgtgcgg ctggggtccg tgccctactc cctgctcttc cgcgtcctgc
     2641 tgcagtgctt gaagcaggag tctgactgga aggtgctgaa gctggttctg ggcaggctgc
     2701 ctgagtccct gcgctataaa gtgctcatct ttacttcccc ttgcagtgtg gaccagctgt
     2761 gctctgctct ctgctccatg ctttcaggcc caaagacact ggagcggctc cgaggcgccc
     2821 cagaaggctt ctccagaact gacttgcacc tggccgtggt tccagtgctg acagcattaa
     2881 tctcttacca taactacctg gacaaaacca aacagcgcga gatggtctac tgcctggagc
     2941 agggcctcat ccaccgctgt gccagccagt gcgtcgtggc cttgtccatc tgcagcgtgg
     3001 agatgcctga catcatcatc aaggcgctgc ctgttctggt ggtgaagctc acgcacatct
     3061 cagccacagc cagcatggcc gtcccactgc tggagttcct gtccactctg gccaggctgc
     3121 cgcacctcta caggaacttt gccgcggagc agtatgccag tgtgttcgcc atctccctgc
     3181 cgtacaccaa cccctccaag tttaatcagt acatcgtgtg tctggcccat cacgtcatag
     3241 ccatgtggtt catcaggtgc cgcctgccct tccggaagga ttttgtccct ttcatcacta
     3301 agggcctgcg gtccaatgtc ctcttgtctt ttgatgacac ccccgagaag gacagcttca
     3361 gggcccggag tactagtctc aacgagagac ccaagagtct gaggatagcc agacccccca
     3421 aacaaggctt gaataactct ccacccgtga aagaattcaa ggagagctct gcagccgagg
     3481 ccttccggtg ccgcagcatc agtgtgtctg aacatgtggt ccgcagcagg atacagacgt
     3541 ccctcaccag tgccagcttg gggtctgcag atgagaactc cgtggcccag gctgacgata
     3601 gcctgaaaaa cctccacctg gagctcacgg aaacctgtct ggacatgatg gctcgatacg
     3661 tcttctccaa cttcacggct gtcccgaaga ggtctcctgt gggcgagttc ctcctagcgg
     3721 gtggcaggac caaaacctgg ctggttggga acaagcttgt cactgtgacg acaagcgtgg
     3781 gaaccgggac ccggtcgtta ctaggcctgg actcggggga gctgcagtcc ggcccggagt
     3841 cgagctccag ccccggggtg catgtaagac agaccaagga ggcgccggcc aagctggagt
     3901 cccaggctgg gcagcaggtg tcccgtgggg cccgggatcg ggtccgttcc atgtcggggg
     3961 gccatggtct tcgagttggc gccctggacg tgccggcctc ccagttcctg ggcagtgcca
     4021 cttctccagg accacggact gcaccagccg cgaaacctga gaaggcctca gctggcaccc
     4081 gggttcctgt gcaggagaag acgaacctgg cggcctatgt gcccctgctg acccagggct
     4141 gggcggagat cctggtccgg aggcccacag ggaacaccag ctggctgatg agcctggaga
     4201 acccgctcag ccctttctcc tcggacatca acaacatgcc cctgcaggag ctgtctaacg
     4261 ccctcatggc ggctgagcgc ttcaaggagc accgggacac agccctgtac aagtcactgt
     4321 cggtgccggc agccagcacg gccaaacccc ctcctctgcc tcgctccaac acagactccg
     4381 ccgtggtcat ggaggaggga agtccgggcg aggttcctgt gctggtggag cccccagggt
     4441 tggaggacgt tgaggcagcg ctaggcatgg acaggcgcac ggatgcctac agcaggtcgt
     4501 cctcagtctc cagccaggag gagaagtcgc tccacgcgga ggagctggtt ggcaggggca
     4561 tccccatcga gcgagtcgtc tcctcggagg gtggccggcc ctctgtggac ctctccttcc
     4621 agccctcgca gcccctgagc aagtccagct cctctcccga gctgcagact ctgcaggaca
     4681 tcctcgggga ccctggggac aaggccgacg tgggccggct gagccctgag gttaaggccc
     4741 ggtcacagtc agggaccctg gacggggaaa gtgctgcctg gtcggcctcg ggcgaagaca
     4801 gtcggggcca gcccgagggt cccttgcctt ccagctcccc ccgctcgccc agtggcctcc
     4861 ggccccgagg ttacaccatc tccgactcgg ccccatcacg caggggcaag agagtagaga
     4921 gggacgcctt aaagagcaga gccacagcct ccaatgcaga gaaagtgcca ggcatcaacc
     4981 ccagtttcgt gttcctgcag ctctaccatt cccccttctt tggcgacgag tcaaacaagc
     5041 caatcctgct gcccaatgag tcacagtcct ttgagcggtc ggtgcagctc ctcgaccaga
     5101 tcccatcata cgacacccac aagatcgccg tcctgtatgt tggagaaggc cagagcaaca
     5161 gcgagctcgc catcctgtcc aatgagcatg gctcctacag gtacacggag ttcctgacgg
     5221 gcctgggccg gctcatcgag ctgaaggact gccagccgga caaggtgtac ctgggaggcc
     5281 tggacgtgtg tggtgaggac ggccagttca cctactgctg gcacgatgac atcatgcaag
     5341 ccgtcttcca catcgccacc ctgatgccca ccaaggacgt ggacaagcac cgctgcgaca
     5401 agaagcgcca cctgggcaac gactttgtgt ccattgtcta caatgactcc ggtgaggact
     5461 tcaagcttgg caccatcaag ggccagttca actttgtcca cgtgatcgtc accccgctgg
     5521 actacgagtg caacctggtg tccctgcagt gcaggaaaga catggagggc cttgtggaca
     5581 ccagcgtggc caagatcgtg tctgaccgca acctgccctt cgtggcccgc cagatggccc
     5641 tgcacgcaaa tatggcctca caggtgcatc atagccgctc caaccccacc gatatctacc
     5701 cctccaagtg gattgcccgg ctccgccaca tcaagcggct ccgccagcgg atctgcgagg
     5761 aagccgccta ctccaacccc agcctacctc tggtgcaccc tccgtcccat agcaaagccc
     5821 ctgcacagac tccagccgag cccacacctg gctatgaggt gggccagcgg aagcgcctca
     5881 tctcctcggt ggaggacttc accgagtttg tgtgatctag aggatccccg gggaattcaa
     5941 tcgatggccg ccatggccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     6001 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6061 caaactcatc aatgtatctt atcatgtctg gatcgggaat taattcggcg cagcaccatg
     6121 gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg gaaagaacca
     6181 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6241 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6301 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6361 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6421 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6481 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctgttaa cagcttggca
     6541 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6601 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6661 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     6721 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     6781 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6841 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6901 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6961 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     7021 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7081 aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc
     7141 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7201 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     7261 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     7321 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     7381 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     7441 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     7501 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7561 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7621 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7681 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7741 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7801 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7861 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7921 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     7981 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8041 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8101 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8161 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8221 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8281 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8341 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8401 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8461 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8521 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8581 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8641 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8701 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8761 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8821 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8881 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     8941 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9001 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9061 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9121 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9181 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9241 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9301 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9361 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9421 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     9481 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     9541 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     9601 gataacaatt tcacacagga aacagctatg acatgattac gaattaattc gagctcgccc
     9661 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     9721 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     9781 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     9841 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     9901 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     9961 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
    10021 tagtcatcgc tattaccatg
//
