LOCUS       sequence-401673-        9642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible WT SOX9 with myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Yang Y, Gomez N, Infarinato N, Adam RC, Sribour M, Baek I, Laurin M,
            Fuchs E
  TITLE     The pioneer factor SOX9 competes for epigenetic factors to switch 
            stem cell fates.
  JOURNAL   Nat Cell Biol. 2023 Jul 24. doi: 10.1038/s41556-023-01184-y.
  PUBMED    37488435
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2023 Jul 24. doi: 10.1038/s41556-023-01184-y."
COMMENT     Sequence Label: plko_TRE3G_Myc-Sox9WT_PGKpuroR
FEATURES             Location/Qualifiers
     source          1..9642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(111..130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      924..1512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1413..1432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1666..1683
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1800..1821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1836..1841,1842..1859,1860..1866)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1874..1890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1879..1901
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1898..1914
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1898..1914
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1933..1953
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1935..1953
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1981..2207
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2208..2388
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2435..2560
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3053..3286
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3471..3515
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3813..3930
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4373..4391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4409..4427
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4445..4463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4481..4499
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4517..4535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4553..4571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4589..4607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4652..4676
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4757..4766
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4766..4795
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        6327..6827
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6746..6764
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6836..7438
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7335..7355
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7455..8043
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7508..7528)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7926..7937)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8141..8374
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8446..8567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8483..8502)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8537..8556
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8607..8742
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8762..8781)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8763..8781)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8791..8808)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8791..8807)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8800..8822)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8949..9404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9036..9055)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9246..9267
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9430..9534
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9535..9603,9604..9642,1..753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
       61 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      121 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      181 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      241 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      301 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      361 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      421 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      481 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
      541 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
      601 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
      661 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
      721 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
      781 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
      841 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
      901 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      961 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1021 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1081 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1141 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1201 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1261 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1321 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1381 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1441 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1501 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1561 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1621 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1681 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1741 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     1801 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     1861 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     1921 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     1981 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     2041 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     2101 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     2161 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     2221 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2281 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2341 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2401 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     2461 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     2521 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     2581 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     2641 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     2701 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     2761 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     2821 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     2881 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     2941 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     3001 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     3061 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     3121 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     3181 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     3241 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     3301 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     3361 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     3421 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     3481 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     3541 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     3601 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     3661 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     3721 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     3781 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     3841 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     3901 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     3961 gtttggttag taccgggccc ggtgctttgc tctgagccag cccaccagtt tggaatgact
     4021 cctttttatg acttgaattt tcaagtataa agtctagtgc taaatttaat ttgaacaact
     4081 gtatagtttt tgctggttgg gggaaggaaa aaaaatggtg gcagtgtttt tttcagaatt
     4141 agaagtgaaa tgaaaacttg ttgtgtgtga ggatttctaa tgacatgtgg tggttgcata
     4201 ctgagtgaag ccggtgagca ttctgccatg tcaccccctc gtgctcagta atgtacttta
     4261 cagaaatcct aaactcaaaa gattgatata aaccatgctt cttgtgtata tccggtctct
     4321 tctctgggta gtctcactca gcctgcattt ctgccatcga tggtaccttt actccctatc
     4381 agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg tatgcagact
     4441 ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag tgatagagaa
     4501 cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt actccctatc
     4561 agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg tataagcttt
     4621 aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt cagatcgcct
     4681 ggagcaattc cacaacactt ttgtcttata ccaactttcc gtaccacttc ctaccctcgt
     4741 aaagctagca ccggtcgcca ccatggaaca aaaacttatt tctgaagaag atctgaatct
     4801 cctggacccc ttcatgaaga tgaccgacga gcaggagaag ggcctgtctg gcgcccccag
     4861 ccccaccatg tcggaggact cggctggttc gccctgtccc tcgggctccg gctcggacac
     4921 ggagaacacc cggccccagg agaacacctt ccccaagggc gagccggatc tgaagaagga
     4981 gagcgaggaa gataagttcc ccgtgtgcat ccgcgaggcg gtcagccagg tgctgaaggg
     5041 ctacgactgg acgctggtgc ccatgcccgt gcgcgtcaac ggctccagca agaacaagcc
     5101 acacgtcaag cgacccatga acgccttcat ggtgtgggcg caggctgcgc gcaggaagct
     5161 ggcagaccag tacccgcatc tgcacaacgc ggagctcagc aagactctgg gcaagctctg
     5221 gaggctgctg aacgagagcg agaagagacc cttcgtggag gaggcggagc ggctgcgcgt
     5281 gcagcacaag aaagaccacc ccgattacaa gtaccagccc cggcggagga agtcggtgaa
     5341 gaacggacaa gcggaggccg aagaggccac ggaacagact cacatctctc ctaatgctat
     5401 cttcaaggcg ctgcaagccg actccccaca ttcctcctcc ggcatgagtg aggtgcactc
     5461 cccgggcgag cactctgggc aatctcaggg tccgccgacc ccacccacca ctcccaaaac
     5521 cgacgtgcaa gctggcaaag ttgatctgaa gcgagagggg cgccctctgg cagagggggg
     5581 cagacagccc cccatcgact tccgcgacgt ggacatcggt gaactgagca gcgacgtcat
     5641 ctccaacatt gagaccttcg acgtcaatga gtttgaccaa tacttgccac ccaacggcca
     5701 cccaggggtt ccggccaccc acggccaggt cacctacact ggcagttacg gcatcagcag
     5761 caccgcaccc acccctgcga ccgcgggcca cgtgtggatg tcgaagcagc aggcgccgcc
     5821 ccctcctccg cagcagcctc cgcaggcccc gcaagcccca caggcgcctc cgcagcagca
     5881 agcacccccg cagcagccgc aggcacccca gcagcagcag gcacacacgc tcaccacgct
     5941 gagcagcgag ccaggccagt cccagcgaac gcacatcaag acggagcagc tgagccccag
     6001 ccactacagc gagcagcagc agcactcccc gcaacagatc tcctacagcc ccttcaacct
     6061 tcctcactac agcccctcct acccgcccat cacccgctcg caatacgact acgctgacca
     6121 tcagaactcc ggctcctact acagtcacgc agccggccag ggctcagggc tctactccac
     6181 cttcacttac atgaaccccg cgcagcgccc catgtacacc cccatcgctg acacctccgg
     6241 ggtcccttcc atcccgcaga cccacagccc gcagcactgg gaacaaccag tctacacaca
     6301 gctcaccaga ccctgaacgc gtggggttgc gccttttcca aggcagccct gggtttgcgc
     6361 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     6421 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     6481 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     6541 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     6601 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     6661 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     6721 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     6781 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatccatggc
     6841 caccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     6901 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     6961 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     7021 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     7081 cgtcgaagcg ggggcggtgt tcgccgagat cggctcgcgc atggccgagt tgagcggttc
     7141 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     7201 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     7261 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgct ggggtgcccg ccttcctgga
     7321 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     7381 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagg
     7441 atcctctaga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7501 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7561 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7621 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7681 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7741 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7801 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     7861 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7921 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7981 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     8041 cgcctgagat cctttaagac caatgcatcg gtacctttaa gaccaatgac ttacaaggca
     8101 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     8161 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     8221 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8281 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8341 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     8401 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     8461 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8521 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     8581 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8641 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8701 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     8761 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     8821 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     8881 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     8941 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     9001 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     9061 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     9121 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     9181 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     9241 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     9301 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     9361 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     9421 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     9481 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     9541 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     9601 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag at
//
