LOCUS       sequence-401674-        8790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible myc tag truncated SOX9 without trans-activating domain
            (noTA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Yang Y, Gomez N, Infarinato N, Adam RC, Sribour M, Baek I, Laurin M,
            Fuchs E
  TITLE     The pioneer factor SOX9 competes for epigenetic factors to switch 
            stem cell fates.
  JOURNAL   Nat Cell Biol. 2023 Jul 24. doi: 10.1038/s41556-023-01184-y.
  PUBMED    37488435
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2023 Jul 24. doi: 10.1038/s41556-023-01184-y."
COMMENT     Sequence Label: plko_TRE3G_Myc-Sox9HMGonlySTOP_PGKpuroR
FEATURES             Location/Qualifiers
     source          1..8790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(111..130)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      924..1512
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1413..1432
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1666..1683
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1800..1821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1836..1841,1842..1859,1860..1866)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1874..1890
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1879..1901
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1898..1914
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1898..1914
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1933..1953
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1935..1953
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1981..2207
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2208..2388
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2435..2560
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3053..3286
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3471..3515
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3813..3930
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4373..4391
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4409..4427
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4445..4463
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4481..4499
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4517..4535
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4553..4571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4589..4607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4652..4676
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4757..4766
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4766..4795
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        5475..5975
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5894..5912
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5984..6586
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6483..6503
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6603..7191
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6656..6676)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7074..7085)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7289..7522
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7594..7715
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7631..7650)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7685..7704
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7755..7890
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7910..7929)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7911..7929)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7939..7956)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7939..7955)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7948..7970)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8097..8552
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8184..8203)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8394..8415
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8578..8682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8683..8751,8752..8790,1..753)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
       61 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      121 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      181 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      241 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      301 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      361 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      421 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      481 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
      541 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
      601 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
      661 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
      721 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
      781 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
      841 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
      901 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      961 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1021 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1081 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1141 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1201 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1261 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1321 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1381 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1441 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1501 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1561 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1621 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1681 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1741 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     1801 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     1861 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     1921 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     1981 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     2041 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     2101 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     2161 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     2221 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2281 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2341 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2401 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     2461 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     2521 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     2581 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     2641 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     2701 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     2761 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     2821 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     2881 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     2941 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     3001 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     3061 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     3121 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     3181 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     3241 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     3301 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     3361 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     3421 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     3481 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     3541 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     3601 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     3661 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     3721 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     3781 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     3841 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     3901 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     3961 gtttggttag taccgggccc ggtgctttgc tctgagccag cccaccagtt tggaatgact
     4021 cctttttatg acttgaattt tcaagtataa agtctagtgc taaatttaat ttgaacaact
     4081 gtatagtttt tgctggttgg gggaaggaaa aaaaatggtg gcagtgtttt tttcagaatt
     4141 agaagtgaaa tgaaaacttg ttgtgtgtga ggatttctaa tgacatgtgg tggttgcata
     4201 ctgagtgaag ccggtgagca ttctgccatg tcaccccctc gtgctcagta atgtacttta
     4261 cagaaatcct aaactcaaaa gattgatata aaccatgctt cttgtgtata tccggtctct
     4321 tctctgggta gtctcactca gcctgcattt ctgccatcga tggtaccttt actccctatc
     4381 agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg tatgcagact
     4441 ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag tgatagagaa
     4501 cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt actccctatc
     4561 agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg tataagcttt
     4621 aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt cagatcgcct
     4681 ggagcaattc cacaacactt ttgtcttata ccaactttcc gtaccacttc ctaccctcgt
     4741 aaagctagca ccggtcgcca ccatggaaca aaaacttatt tctgaagaag atctgaatct
     4801 cctggacccc ttcatgaaga tgaccgacga gcaggagaag ggcctgtctg gcgcccccag
     4861 ccccaccatg tcggaggact cggctggttc gccctgtccc tcgggctccg gctcggacac
     4921 ggagaacacc cggccccagg agaacacctt ccccaagggc gagccggatc tgaagaagga
     4981 gagcgaggaa gataagttcc ccgtgtgcat ccgcgaggcg gtcagccagg tgctgaaggg
     5041 ctacgactgg acgctggtgc ccatgcccgt gcgcgtcaac ggctccagca agaacaagcc
     5101 acacgtcaag cgacccatga acgccttcat ggtgtgggcg caggctgcgc gcaggaagct
     5161 ggcagaccag tacccgcatc tgcacaacgc ggagctcagc aagactctgg gcaagctctg
     5221 gaggctgctg aacgagagcg agaagagacc cttcgtggag gaggcggagc ggctgcgcgt
     5281 gcagcacaag aaagaccacc ccgattacaa gtaccagccc cggcggagga agtcggtgaa
     5341 gaacggacaa gcggaggccg aagaggccac ggaacagact cacatctctc ctaatgctat
     5401 cttcaaggcg ctgcaagccg actccccaca ttcctcctcc ggcatgagtg aggtgcactc
     5461 ctgaacgcgt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     5521 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     5581 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     5641 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     5701 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     5761 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     5821 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     5881 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct
     5941 ccctcgttga ccgaatcacc gacctctctc cccaggggga tccatggcca ccgagtacaa
     6001 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc
     6061 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg
     6121 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6181 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6241 ggcggtgttc gccgagatcg gctcgcgcat ggccgagttg agcggttccc ggctggccgc
     6301 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6361 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6421 ccccggagtg gaggcggccg agcgcgctgg ggtgcccgcc ttcctggaga cctccgcgcc
     6481 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6541 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaggat cctctagatc
     6601 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6661 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6721 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6781 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6841 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6901 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6961 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7021 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7081 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7141 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc
     7201 tttaagacca atgcatcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7261 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7321 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7381 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7441 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7501 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     7561 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7621 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7681 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     7741 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7801 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7861 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     7921 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7981 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8041 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     8101 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8161 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8221 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8281 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8341 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8401 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8461 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8521 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     8581 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8641 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8701 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8761 acgctggtga aagtaaaaga tgctgaagat
//
