LOCUS       sequence-401675-       12452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TVA receptor, rabies oG and "spaghetti monster" 
            fluorescent protein in mature excitatory neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Chiola S, Napan KL, Wang Y, Lazarenko RM, Armstrong CJ, Cui J, 
            Shcheglovitov A
  TITLE     Defective AMPA-mediated synaptic transmission and morphology in 
            human neurons with hemizygous SHANK3 deletion engrafted in mouse 
            prefrontal cortex.
  JOURNAL   Mol Psychiatry. 2021 Feb 8. pii: 10.1038/s41380-021-01023-2. doi: 
            10.1038/s41380-021-01023-2.
  PUBMED    33558651
REFERENCE   2  (bases 1 to 12452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Psychiatry. 2021 Feb 8. pii: 10.1038/s41380-021-01023-2. doi: 
            10.1038/s41380-021-01023-2."
COMMENT     Sequence Label: CamKIIa:smFP-TVA-RGV
FEATURES             Location/Qualifiers
     source          1..12452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        105..152
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             171..545
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    675..796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(712..731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     766..785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(845..861)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(845..861)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(858..880)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    869..885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(893..899,900..917,918..923))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    938..959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1076..1093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1247..1835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1327..1346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2006..2797,2798..2866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2629..2648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2867..2971)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3046..3065)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3237..3616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3618..3816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3771..3791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3834..4014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4061..4186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4679..4912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5097..5141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5439..5556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5601..6889
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      6912..6921
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6921..6944
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6948..6971
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6975..6998
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7527..7550
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7557..7580
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7593..7616
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7623..7646
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7857..7880
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7884..7907
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7911..7934
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7944..8009
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             8490..8543
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    10143..10731
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10196..10216)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10614..10625)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10734..10750)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10735..10751)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11256..11436
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11462..11479)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11468..11692
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11738..12166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11825..11844)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12035..12056
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12175..12195)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(12180..12452,1..57)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(12360..12452,1..43)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12422..12441
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
       61 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
      121 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
      181 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
      241 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
      301 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
      361 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
      421 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
      481 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
      541 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
      601 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
      661 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      721 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      781 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
      841 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
      901 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
      961 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     1021 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1681 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1921 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1981 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2041 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2101 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2161 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2221 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2281 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2341 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2401 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2461 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2521 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2581 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2641 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     2701 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     2761 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2821 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2881 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2941 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     3001 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     3061 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     3121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     3181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     3241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     3301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     3361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     3421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     3481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     3541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     3601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     3661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     3721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     3781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
     3841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     3901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     3961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     4021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     4081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     4141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     4201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     4261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     4321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     4381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     4441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     4501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     4561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     4621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     4681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     4741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     4801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     4861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     4921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     4981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     5041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     5101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     5161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     5221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     5281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     5341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     5401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     5461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     5521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     5581 gatccagttt ggttaattaa cattatggcc ttaggtcact tcatctccat ggggttcttc
     5641 ttctgatttt ctagaaaatg agatgggggt gcagagagct tcctcagtga cctgcccagg
     5701 gtcacatcag aaatgtcaga gctagaactt gaactcagat tactaatctt aaattccatg
     5761 ccttgggggc atgcaagtac gatatacaga aggagtgaac tcattagggc agatgaccaa
     5821 tgagtttagg aaagaagagt ccagggcagg gtacatctac accacccgcc cagccctggg
     5881 tgagtccagc cacgttcacc tcattatagt tgcctctctc cagtcctacc ttgacgggaa
     5941 gcacaagcag aaactgggac aggagcccca ggagaccaaa tcttcatggt ccctctggga
     6001 ggatgggtgg ggagagctgt ggcagaggcc tcaggagggg ccctgctgct cagtggtgac
     6061 agataggggt gagaaagcag acagagtcat tccgtcagca ttctgggtct gtttggtact
     6121 tcttctcacg ctaaggtggc ggtgtgatat gcacaatggc taaaaagcag ggagagctgg
     6181 aaagaaacaa ggacagagac agaggccaag tcaaccagac caattcccag aggaagcaaa
     6241 gaaaccatta cagagactac aagggggaag ggaaggagag atgaattagc ttcccctgta
     6301 aaccttagaa cccagctgtt gccagggcaa cggggcaata cctgtctctt cagaggagat
     6361 gaagttgcca gggtaactac atcctgtctt tctcaaggac catcccagaa tgtggcaccc
     6421 actagccgtt accatagcaa ctgcctcttt gccccactta atcccatccc gtctgttaaa
     6481 agggccctat agttggaggt gggggaggta ggaagagcga tgatcacttg tggactaagt
     6541 ttgttcgcat ccccttctcc aaccccctca gtacatcacc ctgggggaac agggtccact
     6601 tgctcctggg cccacacagt cctgcagtat tgtgtatata aggccagggc aaagaggagc
     6661 aggttttaaa gtgaaaggca ggcaggtgtt ggggaggcag ttaccggggc aacgggaaca
     6721 gggcgtttcg gaggtggttg ccatggggac ctggatgctg acgaaggctc gcgaggctgt
     6781 gagcagccac agtgccctgc tcagaagccc caagctcgtc agtcaagccg gttctccgtt
     6841 tgcactcagg agcacgggca ggcgagtggc ccctagttct gggggcagcg ggggatcccc
     6901 gggtaccggt cgccaccatg gactacaagg acgacgacga taaaggagat tacaaagatg
     6961 acgatgataa gggcgattat aaggacgacg atgacaaggg cggcgtgtcg aaaggcgagg
     7021 agctgttcac aggcgtggtc cccatcctgg ttgagctgga cggtgacgtt aacggccaca
     7081 aatttagcgt acgtggtgag ggcgagggtg acgctaccaa cggaaagctg accctgaaat
     7141 tcatctgcac aactggcaag ctgccggtgc cctggcccac gctggtaacc actctgggcg
     7201 gaggcgttca atgtttcagt cgttacccgg atcacatgaa gcagcatgac ttcttcaagt
     7261 cggccatgcc cgaaggctac gtgcaggagc gcaccatcag ttttaaggat gacggcacct
     7321 acaagactcg cgccgaggtc aagttcgaag gagacaccct ggttaatcgt atcgagctca
     7381 aaggcatcga tttcaaggag gacggaaata ttctgggcca caagctggag tacaatttta
     7441 acagccacaa tgtttacatt actgccgaca agcagaagaa cggcatcaag gctaatttca
     7501 agattcgtca caatgtggag ggaggcgact ataaggatga cgatgacaaa caacaagact
     7561 ataaggacga tgatgacaaa ggtcagcaag gcgattacaa ggacgatgac gataagcagc
     7621 aagattacaa ggatgatgat gataagggag gcgacggaag cgtgcagctg gcagatcatt
     7681 accaacagaa cacgcccatt ggtgacggtc cagtcctgct gcctgataac cactacttgt
     7741 ccacccagtc ggtgctgtcg aaggacccga acgagaagcg tgaccacatg gtgctgctgg
     7801 agttcgtgac cgccgcagga atcaccctcg gcatggatga gctttacaag ggcggagatt
     7861 ataaagatga cgacgacaag ggcgactaca aagatgatga tgacaagggt gactacaaag
     7921 acgacgatga taaaggtagt ggtgtgaaac agactttgaa ttttgacctt ctcaagttgg
     7981 cgggagacgt ggagtccaac ccagggccca tggcgcggct gctgcccgcg ctgctgctgc
     8041 tgctgctgcc cggtaacgtg accggtaacg ggtccggtaa cggttctttg tcccgttgcc
     8101 cccccggtca gttccgctgc tcggagccgc ccggtgccca cggggagtgt tacccgcagg
     8161 actggctgtg cgacggacac cccgactgcg acgacgggcg ggacgagtgg ggctgcggga
     8221 ccagcgcgat ccccgcggtg cccacggaca acggcacaga ggctcccact gcccctgctc
     8281 ctggacgtgc tctgccagcc aggaatcacg gccgcatgtg gatgctgatc actgcagtgc
     8341 tcctgtgctg cctggtagct gtgggtggta tcgctgcatg ggggaagtcc aaagcaaaaa
     8401 gcaggtctga catcttcagt cttgaaagcg catccaagga gctgctggtg cctgacaaga
     8461 gccaggcaga cttgttctcc ggtagtggag agggcagagg aagtctgcta acatgcggtg
     8521 acgtcgagga gaatcctggc ccaatggtcc cacaggctct cctcttcgtc cctctgctcg
     8581 tcttcccact ctgctttggt aaattcccta tctacactat ccccgacaag ctgggcccct
     8641 ggagtcctat cgatattcac catctgtcat gccctaacaa tctcgtggtc gaggacgaag
     8701 gctgtaccaa cctgagcgga ttctcctaca tggagctgaa agtgggctat atctctgcta
     8761 ttaagatgaa cgggttcaca tgcactggtg tggtcaccga ggcagaaacc tacacaaatt
     8821 ttgtgggata tgtcaccaca actttcaaga gaaaacactt tcggccaaca ccagacgcct
     8881 gtcgggcagc ttacaactgg aagatggctg gcgacccccg ctatgaggaa agcctgcaca
     8941 atccttaccc agactatcat tggctgcgca ctgtgaagac cacaaaagag agcctggtca
     9001 tcatttcccc atctgtcgcc gacctggacc cctacgaccg atctctccac agtccagtgt
     9061 ttcccggcgg aaactgctcc ggagtggccg tcagctccac ttactgttcc accaaccatg
     9121 attatacaat ctggatgcct gagaatccaa ggctggggat gtcctgcgac atcttcacca
     9181 acagcagggg caagagggcc tcaaaaggca gcgagacttg tggatttgtg gatgaaagag
     9241 ggctgtataa gagcctcaaa ggtgcctgca agctgaaact ctgtggcgtg ctgggactcc
     9301 ggctgatgga cggaacctgg gtcgctatgc agacatctaa cgagactaag tggtgccccc
     9361 ctgggcagct cgtgaatctg cacgacttcc gaagcgatga gatcgaacat ctggtggtcg
     9421 aggaactcgt gaagaaaagg gaggaatgtc tcgatgctct ggagtcaatt atgactacca
     9481 agagcgtgag cttcaggagg ctgagccacc tgaggaagct cgtccctggc ttcggaaaag
     9541 catacaccat ctttaacaag acactgatgg aggcagacgc ccattataaa tctgtgagaa
     9601 cctggaatga aatcattcct agtaagggat gcctgcgagt cgggggtcga tgtcacccac
     9661 atgtgaacgg ggtcttcttt aatggtatca ttctggggcc agatggtaac gtgctgatcc
     9721 ccgagatgca gtctagtctg ctccagcagc acatggaact gctcgtgtca agcgtcattc
     9781 ccctgatgca tcctctcgca gacccaagca cagtgttcaa aaacggcgac gaggccgaag
     9841 attttgtgga ggtccacctg cccgacgtgc acgaacggat cagtggcgtc gatctcggac
     9901 tgcctaattg gggcaagtac gtgctgctct ccgctggagc actgactgct ctcatgctga
     9961 tcattttcct gatgacctgc tggcgacgag tgaaccgctc cgagccaact cagcacaatc
    10021 tgagggggac cggtagagaa gtgtctgtca caccccagag cggtaaaatc atcagcagtt
    10081 gggaaagtca taaatctggg ggcgagacac ggctctaaga attcgatatc aagcttatcg
    10141 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
    10201 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
    10261 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
    10321 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
    10381 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
    10441 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
    10501 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
    10561 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
    10621 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    10681 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
    10741 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
    10801 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
    10861 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
    10921 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
    10981 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
    11041 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
    11101 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
    11161 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
    11221 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
    11281 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
    11341 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
    11401 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
    11461 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
    11521 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
    11581 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
    11641 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
    11701 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
    11761 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    11821 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    11881 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    11941 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    12001 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    12061 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    12121 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
    12181 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    12241 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
    12301 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
    12361 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
    12421 ttatttatgc agaggccgag gccgcctctg cc
//
