LOCUS       sequence-401677-       10444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad host range IncP promoter probe using lacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Spaink HP, Okker RJ, Wijffelman CA, Pees E, Lugtenberg BJ
  TITLE     Promoters in the nodulation region of the Rhizobium leguminosarum 
            Sym plasmid pRL1JI.
  JOURNAL   Plant Mol Biol. 1987 Jan;9(1):27-39. doi: 10.1007/BF00017984.
  PUBMED    24276795
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Mol Biol"; date: "1987-01"; volume: "9"; issue: "1"; pages: 
            "27-39"
COMMENT     Sequence Label: pMP220
FEATURES             Location/Qualifiers
     source          1..10444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1075..1097
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1191..1210
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     misc_feature    1309..1365
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     rep_origin      1471..2102
                     /label=oriV
                     /note="incP origin of replication"
     oriT            2255..2364
                     /note="incP origin of transfer"
     CDS             2397..2768
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     CDS             3040..4188
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(5461..6660)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             6766..7416
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             complement(join(8496..10444,1..1099))
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
ORIGIN
        1 cctgcaccat cgtctgctca tccatgacct gaccatgcag aggatgatgc tcgtgacggt
       61 taacgcctcg aatcagcaac ggcttgccgt tcagcagcag cagaccattt tcaatccgca
      121 cctcgcggaa accgacatcg caggcttctg cttcaatcag cgtgccgtcg gcggtgtgca
      181 gttcaaccac cgcacgatag agattcggga tttcggcgct ccacagtttc gggttttcga
      241 cgttcagacg tagtgtgacg cgatcggcat aaccaccacg ctcatcgata atttcaccgc
      301 cgaaaggcgc ggtgccgctg gcgacctgcg tttcaccctg ccataaagaa actgttaccc
      361 gtaggtagtc acgcaactcg ccgcacatct gaacttcagc ctccagtaca gcgcggctga
      421 aatcatcatt aaagcgagtg gcaacatgga aatcgctgat ttgtgtagtc ggtttatgca
      481 gcaacgagac gtcacggaaa atgccgctca tccgccacat atcctgatct tccagataac
      541 tgccgtcact ccagcgcagc accatcaccg cgaggcggtt ttctccggcg cgtaaaaatg
      601 cgctcaggtc aaattcagac ggcaaacgac tgtcctggcc gtaaccgacc cagcgcccgt
      661 tgcaccacag atgaaacgcc gagttaacgc catcaaaaat aattcgcgtc tggccttcct
      721 gtagccagct ttcatcaaca ttaaatgtga gcgagtaaca acccgtcgga ttctccgtgg
      781 gaacaaacgg cggattgacc gtaatgggat aggtcacgtt ggtgtagatg ggcgcatcgt
      841 aaccgtgcat ctgccagttt gaggggacga cgacagtatc ggcctcagga agatcgcact
      901 ccagccagct ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc cattcgccat
      961 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     1021 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     1081 cacgacgttg taaaacgacg ggatccggat aaaacttgtg cttatttttc tttacggtct
     1141 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     1201 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     1261 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgtcgaa gcttgcatgc
     1321 ctgcaggtcg actctagagg atccccgggt accgagctcg aattcatcga tatctagatc
     1381 tcgagctcgc gaaagcttgg ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg
     1441 cagcccctgg ggggatggga ggcccgcgtt agcgggccgg gagggttcga gaaggggggg
     1501 cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt
     1561 ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc
     1621 ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt
     1681 gcgcccctca tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt
     1741 cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca
     1801 tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc
     1861 tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt
     1921 cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga
     1981 ggtatccaca acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg
     2041 cgtttgcagg gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg
     2101 tcggaaaggg tcgacggatc ttttccgctg cataaccctg cttcggggtc attatagcga
     2161 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg
     2221 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac
     2281 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga
     2341 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg
     2401 ctgatgaaac caagccaacc aggaagggca gcccacctat caaggtgtac tgccttccag
     2461 acgaacgaag agcgattgag gaaaaggcgg cggcggccgg catgagcctg tcggcctacc
     2521 tgctggccgt cggccagggc tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg
     2581 agctggcccg catcaatggc gacctgggcc gcctgggcgg cctgctgaaa ctctggctca
     2641 ccgacgaccc gcgcacggcg cggttcggtg atgccacgat cctcgccctg ctggcgaaga
     2701 tcgaagagaa gcaggacgag cttggcaagg tcatgatggg cgtggtccgc ccgagggcag
     2761 agccatgact tttttagccg ctaaaacggc cggggggtgc gcgtgattgc caagcacgtc
     2821 cccatgcgct ccatcaagaa gagcgacttc gcggagctgg tattcgtgca gggcaagatt
     2881 cggaatacca agtacgagaa ggacggccag acggtctacg ggaccgactt cattgccgat
     2941 aaggtggatt atctggacac caaggcacca ggcgggtcaa atcaggaata agggcacatt
     3001 gccccggcgt gagtcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg
     3061 aaggcataca ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc
     3121 gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc
     3181 cagcaagcta cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc
     3241 gcgccatcgg ccgccgtgga gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg
     3301 aagtcgatga ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc
     3361 gaggacctgg caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag
     3421 cagcagatca aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg
     3481 cgagcgatgc caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc
     3541 ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc
     3601 tacaccggcg tcgagctgcg ggccgacgat gacgaactgg tgtggcagca ggtgttggag
     3661 tacgcgaagc gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag
     3721 gacctgggct ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc
     3781 ctacaggcga cggcgatggg cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg
     3841 ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg
     3901 atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag
     3961 aagtaccgca agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg
     4021 gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc
     4081 gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg
     4141 gtggaacacg cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg
     4201 tcagttccgg ctgggggttc agcagccagc gctttactgg catttcagga acaagcgggc
     4261 actgctcgac gcacttgctt cgctcagtat cgctcgggac gcacggcgcg ctctacgaac
     4321 tgccgataaa cagaggatta aaattgacaa ttgtgattaa ggctcagatt cgacggcttg
     4381 gagcggccga cgtgcaggat ttccgcgaga tccgattgtc ggccctgaag aaagctccag
     4441 agatgttcgg gtccgtttac gagcacgagg agaaaaagcc catggaggcg ttcgctgaac
     4501 ggttgcgaga tgccgtggca ttcggcgcct acatcgacgg cgagatcatt gggctgtcgg
     4561 tcttcaaaca ggaggacggc cccaaggacg ctcacaaggc gcatctgtcc ggcgttttcg
     4621 tggagcccga acagcgaggc cgaggggtcg ccggtatgct gctgcgggcg ttgccggcgg
     4681 gtttattgct cgtgatgatc gtccgacaga ttccaacggg aatctggtgg atgcgcatct
     4741 tcatcctcgg cgcacttaat atttcgctat tctggagctt gttgtttatt tcggtctacc
     4801 gcctgccggg cggggtcgcg gcgacggtag gcgctgtgca gccgctgatg gtcgtgttca
     4861 tctctgccgc tctgctaggt agcccgatac gattgatggc ggtcctgggg gctatttgcg
     4921 gaactgcggg cgtggcgctg ttggtgttga caccaaacgc agcgctagat cctgtcggcg
     4981 tcgcagcggg cctggcgggg gcggtttcca tggcgttcgg aaccgtgctg acccgcaagt
     5041 ggcaacctcc cgtgcctctg ctcaccttta ccgcctggca actggcggcc ggaggacttc
     5101 tgctcgttcc agtagcttta gtgtttgatc cgccaatccc gatgcctaca ggaaccaatg
     5161 ttctcggcct ggcgtggctc ggcctgatcg gagcgggttt aacctacttc ctttggttcc
     5221 gggggatctc gcgactcgaa cctacagttg tttccttact gggctttctc agcccgggga
     5281 ccgccgtgtt gctaggatgg ttgttcttgg atcagacgct gagtgcgctt caaatcatcg
     5341 gcgtcctgct cgtgatcggg agtatctggc tgggccaacg ttccaaccgc actcctaggg
     5401 cgcgtatagc ttgccggaag tcgccttgac ccgcatggca taggcctatc gtttccacga
     5461 tcagcgatcg gctcgttgcc ctgcgccgct ccaaagcccg cgacgcagcg ccggcaggca
     5521 gagcaagtag agggcagcgc ctgcaatcca tgcccacccg ttccacgttg ttatagaagc
     5581 cgcatagatc gccgtgaaga ggaggggtcc gacgatcgag gtcaggctgg tgagcgccgc
     5641 cagtgagcct tgcagctgcc cctgacgttc ctcatccacc tgcctggaca acattgcttg
     5701 cagcgccggc attccgatgc cacccgaagc aagcaggacc atgatcggga acgccatcca
     5761 tccccgtgtc gcgaaggcaa gcaggatgta gcctgtgccg tcggcaatca ttccgagcat
     5821 gagtgcccgc ctttcgccga gccgggcggc tacagggccg gtgatcattg cctgggcgag
     5881 tgaatgcaga atgccaaatg cggcaagcga aatgccgatc gtggtcgcgt cccagtgaaa
     5941 gcgatcctcg ccgaaaatga cccaaagcgc ggccggcacc tgtccgacaa gttgcatgat
     6001 gaagaagacc gccatcaggg cggcgacgac ggtcatgccc cgggcccacc ggaacgaagc
     6061 gagcgggttg agagcctccc ggcgtaacgg ccggcgttcg cctttgtgcg actccggcaa
     6121 aaggaaacag cccgtcagga aattgaggcc gttcaaggct gccgcggcga agaacggagc
     6181 gtggggggag aaaccgccca tcagcccacc gagcacaggt cccgcgacca tcccgaaccc
     6241 gaaacaggcg ctcatgaagc cgaagtgccg cgcgcgctca tcgccatcag tgatatcggc
     6301 aatataagcg ccggctaccg ccccagtcgc cccggtgatg ccggccacga tccgcccgat
     6361 atagagaacc caaaggaaag gcgctgtcgc catgatggcg tagtcgacag tggcgccggc
     6421 cagcgagacg agcaagattg gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg
     6481 tgcgcaggca aattgcacca acgcatacag cgccagcaga atgccatagt gggcggtgac
     6541 gtcgttcgag tgaaccagat cgcgcaggag gcccggcagc accggcataa tcaggccgat
     6601 gccgacagcg tcgagcgcga cagtgctcag aattacgatc aggggtatgt tgggtttcac
     6661 gtctggcctc cggaccagcc tccgctggtc cgattgaacg cgcggattct ttatcactga
     6721 taagttggtg gacatattat gtttatcagt gataaagtgt caagcatgac aaagttgcag
     6781 ccgaatacag tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt agacggtctg
     6841 acgacacgca aactggcgga acggttgggg gttcagcagc cggcgcttta ctggcacttc
     6901 aggaacaagc gggcgctgct cgacgcactg gccgaagcca tgctggcgga gaatcatacg
     6961 cattcggtgc cgagagccga cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc
     7021 ttcaggcagg cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg
     7081 ggcgcaccgc agatggaaac ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt
     7141 tcggccgggg acgccgtcaa tgcgctgatg acaatcagct acttcactgt tggggccgtg
     7201 cttgaggagc aggccggcga cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct
     7261 ccgctctcgc cgctgttgcg ggccgcgata gacgccttcg acgaagccgg tccggacgca
     7321 gcgttcgagc agggactcgc ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc
     7381 aggaacgttg aaggaccgag aaagggtgac gattgatcag gaccgctgcc ggagcgcaac
     7441 ccactcacta cagcagagcc atgtagacaa catcccctcc ccctttccac cgcgtcagac
     7501 gcccgtagca gcccgctacg ggctttttca tgccctgccc tagcgtccaa gcctcacggc
     7561 cgcgctcggc ctctctggcg gccttctggc gctcctgctg cggcgtccgc tcgtgggccg
     7621 tggcgcgggt ccgcgcgccg gcctcgtgcg cctggcgctc gcgggcgagg tccagggcgg
     7681 ccgtcttcac gttctgcctt gcgcagatga gatagatcca gtctgcaagt gtcagaaacg
     7741 agtctgcatt tgatgccctg aacgtcttca tacatctgat aagcccctcc cggaagtctg
     7801 ctttctctcc gtcagaaagc ataccctgac cggtttcaat cacggaccgt tctgtcaggt
     7861 aatgctctgt ccagtcatac agagccgggt ttgtcacccg tataagctga agcaggcaca
     7921 aatcagggaa ataaacaaaa tcccgcatcc ccggataaag aaaaatcagg gaattaatgg
     7981 cctgatggat ttcccgtggc gtcgaaagtg cggcaccata ggtatcagtc accgcgatga
     8041 gatcccttac cattccagag tctggcggtt gattattaat ttgctgatat agagcctcag
     8101 cccgctggcg aaattcatta cgtaaatcaa aggcttcagg tcggggtaat ttaaaactaa
     8161 gctgaatgat tttctggaga taacggctgc catcttcgat attcagcgca tgttcaacgg
     8221 catgagtgat aatctgcctg tcataacaga gaatatgggt aaagcggggc agatcggcta
     8281 ctgcacgcac aagcctgaac acttccgcca cctgggatgg ctccagtcgg tccagatcat
     8341 ccatgacaac aatgaacttc agatccagac tcaccagttg tcctgcaatt tcagcccgaa
     8401 gctagctttc gcgagctcga gatcgatcta gataaatagt acataatgga tttccttacg
     8461 cgaaatacgg gcagacatgg cctgcccggt tattattatt tttgacacca gaccaactgg
     8521 taatggtagc gaccggcgct cagctgtaat tccgccgata ctgacgggct ccaggagtcg
     8581 tcgccaccaa tccccatatg gaaaccgtcg atattcagcc atgtgccttc ttccgcgtgc
     8641 agcagatggc gatggctggt ttccatcagt tgctgttgac tgtagcggct gatgttgaac
     8701 tggaagtcgc cgcgccactg gtgtgggcca taattcaatt cgcgcgtccc gcagcgcaga
     8761 ccgttttcgc tcgggaagac gtacggggta tacatgtctg acaatggcag atcccagcgg
     8821 tcaaaacagg cggcagtaag gcggtcggga tagttttctt gcggccctaa tccgagccag
     8881 tttacccgct ctgctacctg cgccagctgg cagttcaggc caatccgcgc cggatgcggt
     8941 gtatcgctcg ccacttcaac atcaacggta atcgccattt gaccactacc atcaatccgg
     9001 taggttttcc ggctgataaa taaggttttc ccctgatgct gccacgcgtg agcggtcgta
     9061 atcagcaccg catcagcaag tgtatctgcc gtgcactgca acaacgctgc ttcggcctgg
     9121 taatggcccg ccgccttcca gcgttcgacc caggcgttag ggtcaatgcg ggtcgcttca
     9181 cttacgccaa tgtcgttatc cagcggtgca cgggtgaact gatcgcgcag cggcgtcagc
     9241 agttgttttt tatcgccaat ccacatctgt gaaagaaagc ctgactggcg gttaaattgc
     9301 caacgcttat tacccagctc gatgcaaaaa tccatttcgc tggtggtcag atgcgggatg
     9361 gcgtgggacg cggcggggag cgtcacactg aggttttccg ccagacgcca ctgctgccag
     9421 gcgctgatgt gcccggcttc tgaccatgcg gtcgcgttcg gttgcactac gcgtactgtg
     9481 agccagagtt gcccggcgct ctccggctgc ggtagttcag gcagttcaat caactgttta
     9541 ccttgtggag cgacatccag aggcacttca ccgcttgcca gcggcttacc atccagcgcc
     9601 accatccagt gcaggagctc gttatcgcta tgacggaaca ggtattcgct ggtcacttcg
     9661 atggtttgcc cggataaacg gaactggaaa aactgctgct ggtgttttgc ttccgtcagc
     9721 gctggatgcg gcgtgcggtc ggcaaagacc agaccgttca tacagaactg gcgatcgttc
     9781 ggcgtatcgc caaaatcacc gccgtaagcc gaccacgggt tgccgttttc atcatattta
     9841 atcagcgact gatccaccca gtcccagacg aagccgccct gtaaacgggg atactgacga
     9901 aacgcctgcc agtatttagc gaaaccgcca agactgttac ccatcgcgtg ggcgtattcg
     9961 caaaggatca gcgggcgcgt ctctccaggt agcgaaagcc attttttgat ggaccatttc
    10021 ggcacagccg ggaagggctg gtcttcatcc acgcgcgcgt acatcgggca aataatatcg
    10081 gtggccgtgg tgtcggctcc gccgccttca tactgcaccg ggcgggaagg atcgacagat
    10141 ttgatccagc gatacagcgc gtcgtgatta gcgccgtggc ctgattcatt ccccagcgac
    10201 cagatgatca cactcgggtg attacgatcg cgctgcacca ttcgcgttac gcgttcgctc
    10261 atcgccggta gccagcgcgg atcatcggtc agacgattca ttggcaccat gccgtgggtt
    10321 tcaatattgg cttcatccac cacatacagg ccgtagcggt cgcacagcgt gtaccacagc
    10381 ggatggttcg gataatgcga acagcgcacg gcgttaaagt tgttctgctt catcagcagg
    10441 atat
//
