LOCUS       sequence-401678-        3852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond 
            isomerase (PDI) constitutively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Hennigan JN, Menacho-Melgar R, Sarkar P, Golovsky M, Lynch MD
  TITLE     
  JOURNAL   Metab Eng. 2024 Sep;85:116-130. doi: 10.1016/j.ymben.2024.07.012. 
            Epub 2024 Jul 24.
  PUBMED    39059674
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2024-09"; volume: "85:116-130. doi"; pages: " 
            10.1016/j.ymben.2024.07.012. Epub 2024 Jul 24"
COMMENT     Sequence Label: pCOLA-Gent-EM7-Erv1p-PDI
FEATURES             Location/Qualifiers
     source          1..3852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(181..816)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(326..345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1009..1056
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     terminator      3172..3199
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        3205..3295
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gaagtgccag acgatggaag gacgcaatga agctggagga ttggcgtggg aatcgtgctt
       61 ctgtctaagc aagaatgcct agcgtacagg gtgcactttg taacgatttg ggagtccaga
      121 gactcgctgt tttcgaaatt ctcggtcgct acgctccggg cgtgagacta tgcagcgcag
      181 aaacgtccta gaagatgcca ggaggatact tagcagagag acaataaggc cggagcgaag
      241 ccgtttttcc ataggctccg cccccctgac gaacatcacg aaatctgacg ctcaaatcag
      301 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctga tggctccctc
      361 ttgcgctctc ctgttcccgt cctgcggcgt ccgtgttgtg gtggaggctt tacccaaatc
      421 accacgtccc gttccgtgta gacagttcgc tccaagctgg gctgtgtgca agaacccccc
      481 gttcagcccg actgctgcgc cttatccggt aactatcatc ttgagtccaa cccggaaaga
      541 cacgacaaaa cgccactggc agcagccatt ggtaactgag aattagtgga tttagatatc
      601 gagagtcttg aagtggtggc ctaacagagg ctacactgaa aggacagtat ttggtatctg
      661 cgctccacta aagccagtta ccaggttaag cagttcccca actgacttaa ccttcgatca
      721 aaccgcctcc ccaggcggtt ttttcgttta cagagcagga tattacgacg atcgtaaaag
      781 gatctcaaga agatccttta cggattcccg acaccatcac tctagatttc agtgcaattt
      841 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta attctcatgt
      901 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
      961 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgtgt tgacaattaa
     1021 tcatcggcat agtatatcgg catagtataa tacgacgtgt aggaggataa tctatgaagg
     1081 caattgataa gatgaccgac aatcctcctc aggaaggctt gtccgggcgc aaaatcatct
     1141 atgatgagga cgggaagccg tgccgttcct gcaatacttt gctggacttc caatacgtca
     1201 cgggtaaaat ctcgaatgga ttgaagaatt tgtcttcaaa tggaaaactg gcagggactg
     1261 gggctcttac gggggaggca tcggaactta tgcctggttc ccgcacttat cgcaaagtag
     1321 atccacctga tgttgagcag cttggccgtt cttcgtggac cttgttgcac tctgtcgccg
     1381 cgtcgtatcc agctcagcct acggaccaac agaagggcga gatgaaacag ttcctgaaca
     1441 tcttctcgca catttaccct tgcaactggt gcgccaagga cttcgagaag tacattcgcg
     1501 agaatgcgcc gcaagtagaa tcccgcgaag aattgggacg ttggatgtgt gaagcacata
     1561 ataaggtgaa taagaagtta cgcaaaccaa agttcgactg taatttctgg gaaaaacgct
     1621 ggaaagacgg gtgggacgag taatgatcta gagattaaag aggagaatac tagatggatg
     1681 cgccagaaga agaagatcat gttcttgtgc ttcgtaaatc caatttcgcg gaagctttgg
     1741 cagcgcataa atatttgctg gttgaattct acgccccttg gtgtggtcat tgcaaggcgt
     1801 tggcgcccga gtatgcgaag gctgctggca agcttaaagc ggagggctcg gaaatccgtt
     1861 tagcgaaagt ggatgccacg gaggaatcag accttgcaca acagtacggc gtccgcggtt
     1921 atccgacgat taaattcttc cgcaatggag atacagcatc cccaaaggag tacacggctg
     1981 ggcgtgaggc agatgacatt gtcaattggc ttaaaaaacg cacaggccca gcggctacga
     2041 ctctgccaga tggtgctgcg gcagaaagtc ttgttgaatc gagtgaagtt gctgtaattg
     2101 gattttttaa ggacgtcgag tcagatagtg ctaagcaatt tttacaagcc gccgaagcaa
     2161 tcgacgacat tcctttcggc atcacaagta attctgatgt cttctcgaag taccaattgg
     2221 acaaggatgg agtcgtgtta tttaaaaagt tcgatgaggg ccgtaataat ttcgaaggcg
     2281 aggtgactaa ggagaactta ttagatttca tcaaacacaa ccaacttcct cttgtcattg
     2341 aattcactga gcaaactgcg ccgaagattt tcgggggaga aattaagacc cacatcttgt
     2401 tgtttctgcc gaaatcagtt tcggactacg atgggaaact gagcaatttt aagactgcgg
     2461 cggaatcatt taaagggaag attttattca tcttcattga cagtgaccac actgacaacc
     2521 agcgtattct ggagttcttc ggattaaaga aagaggaatg tccagccgta cgtttgatta
     2581 ctctggagga ggagatgact aaatacaaac ccgagtctga agagctgaca gcggagcgta
     2641 ttaccgagtt ctgccatcgc tttttagaag gcaaaattaa gccacatctt atgtcgcaag
     2701 aactgccgga agactgggac aagcagcctg tcaaggtgct tgtcggaaaa aacttcgagg
     2761 atgttgcgtt tgacgagaag aagaatgtct ttgttgagtt ctacgccccc tggtgcgggc
     2821 attgtaaaca gttggccccc atttgggaca agcttggcga gacgtacaaa gaccatgaga
     2881 atattgtaat tgcgaaaatg gattcaaccg caaatgaggt cgaagctgtg aaagtacaca
     2941 gcttcccaac actgaaattt tttcctgcat cggccgatcg cacagttatc gactacaacg
     3001 gagaacgtac cctggacggc tttaaaaaat tcctggagtc tgggggtcag gatggtgctg
     3061 gggatgacga cgacttagag gaccttgagg aagccgaaga acccgacatg gaggaggatg
     3121 atgatcagaa ggcggttaaa gatgagttgt aatgaggatc cgagtcacac tggctcacct
     3181 tcgggtgggc ctttctgcgt ttattgatcg gcacgtaaga ggttccaact ttcaccataa
     3241 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctggaag
     3301 gaagtaaaaa tgttgcgtag ctctaacgat gtgacgcaac aaggttcgcg tccaaagaca
     3361 aaattgggag gcagtagcat ggggatcatt cgcacttgtc gcctggggcc agaccaggtg
     3421 aagtcaatgc gtgcggctct ggacttattc gggcgcgaat ttggagatgt agccacttac
     3481 tcacagcacc aaccggacag tgattacttg gggaatttac ttcgcagtaa aacttttatc
     3541 gctttggccg ctttcgacca ggaggctgta gtaggtgcgt tggcagccta tgttcttcct
     3601 aaattcgagc aaccgcgtag cgaaatttac atctatgatc ttgcagtctc cggcgaacat
     3661 cgccgtcagg ggatcgccac agctttaatc aaccttttga agcatgaggc taatgcactt
     3721 ggagcgtacg tgatttatgt gcaggctgac tacggtgatg atcctgcagt cgctctgtac
     3781 accaaactgg gtatccgcga ggaggtcatg cactttgata ttgacccgtc tacggctacc
     3841 taatagaagc tt
//
