LOCUS       sequence-401688-        1947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vasa expression in Schneider cells for imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: pAc5.1-EGFP-Vasa open (K295N) (RR228)
FEATURES             Location/Qualifiers
     source          1..1947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(338..355)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgtctgacg actgggatga tgagcccatt gttgatactc gcggcgcccg cggtggagat
       61 tggagcgatg atgaggacac ggccaagagc ttcagcggcg aagctgaagg cgatggtgtt
      121 ggagggagcg gtggtgaagg cggcggctac caaggaggaa atcgagatgt gttcggaagg
      181 atcggcggag gcagaggagg aggagctgga ggttatcgag gaggaaatcg agatggaggg
      241 ggcttccacg gtggacgtcg cgagggagaa agggacttcc gcggtggaga aggcggcttc
      301 cgcggtggac aaggcggctc ccgcggtgga caaggcggct cccgcggggg acaaggcggc
      361 ttccgtggtg gagaaggcgg cttccgcggt cggctgtacg aaaacgagga tggtgatgaa
      421 cgtcgtggaa gactagatcg ggaggaacgc ggcggtgaac gtggtgaaag aggggacggt
      481 ggttttgcga ggcgccgtcg aaatgaagat gatataaata ataataataa tatcgccgaa
      541 gatgtcgaaa ggaagcgaga gttttatatt cctcccgagc cgtccaacga tgcaattgag
      601 atattcagca gtggcatcgc ttccgggatt catttttcga aatacaacaa cataccggtt
      661 aaggtgaccg gcagcgatgt tccacaacct atacaacatt ttacgagcgc tgatttgaga
      721 gatattatta tagacaatgt aaacaaatct ggatacaaaa taccgacacc aatacaaaag
      781 tgttccatac cagtaatatc ctctggtcga gacctaatgg cgtgtgccca gacgggttct
      841 ggaaacacgg cagctttttt gttgcccatt ttgagcaagt tgttggagga tccccatgaa
      901 ctggagcttg gaagacccca ggtagtgatt gtatccccaa caagggagct ggcaattcag
      961 atctttaacg aggcgaggaa gtttgctttc gagtcatatc taaagatcgg tattgtttac
     1021 ggaggcacct cgttcagaca tcaaaacgag tgcattacca gaggctgcca tgtagtgatc
     1081 gccactccgg gacgacttct ggatttcgtg gatcggacct ttatcacgtt tgaagacact
     1141 cgattcgttg tgctggatga ggccgatcgc atgctagata tgggtttctc agaagacatg
     1201 cgaagaatca tgacgcatgt aactatgcgt ccagaacatc agacattgat gttttccgcc
     1261 acgtttccag aagaaatcca aagaatggcc ggcgaattcc ttaaaaatta cgttttcgtc
     1321 gccattggca ttgtaggcgg agcttgctct gatgtgaagc agaccatata cgaagttaat
     1381 aagtacgcca agcgatccaa gctaatagaa atcctttccg agcaagcaga tggcaccatt
     1441 gtgtttgtgg agacaaagcg tggcgccgac tttttagctt ccttcctgtc agaaaaggag
     1501 tttccgacga cctccattca tggcgatcgt ctccagagtc aacgcgagca ggccttgcgt
     1561 gatttcaaga acggctctat gaaggttctc atagccactt cagtggcttc tcgtggatta
     1621 gacattaaaa acatcaagca tgtgatcaac tatgacatgc ccagcaagat cgatgactac
     1681 gtacatcgca ttggacgtac aggtcgtgta ggcaataatg gacgagccac aagcttcttt
     1741 gaccctgaaa aggatcgagc tattgctgcg gacttggtaa aaatcttgga gggatctggc
     1801 cagactgttc cggactttct acgcacctgt ggtgccggcg gtgatggggg ttactccaat
     1861 caaaatttcg gtggggtgga tgtgcgtggc aggggaaatt acgtcggcga tgccaccaat
     1921 gtcgaggaag aagagcaatg ggattga
//
