LOCUS       sequence-401704-         282 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRB expression in Schneider cells for imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: pAc5.1-mEGFP-human FRB (HK168)
FEATURES             Location/Qualifiers
     source          1..282
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..282
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISK"
ORIGIN
        1 atgatcctct ggcatgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt
       61 ggggaaagga acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa
      121 cggggccccc agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg
      181 gaggcccaag agtggtgcag gaagtacatg aaatcaggga atgtcaagga cctcacccaa
      241 gcctgggacc tctattatca tgtgttccga cgaatctcaa ag
//
