LOCUS       sequence-401747-       14039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tudor expression in Schneider cells for imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14039)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 14039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: pAc5.1-PH-mCherry-Tudor isoform A (HK130)
FEATURES             Location/Qualifiers
     source          1..14039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2987..3694
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3132..3150)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3400..3419
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             11324..11365
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             11375..11392
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(11415..11432)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11465..11599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11489..11508)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11543..11562
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(12221..12809)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12301..12320)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12980..13771,13772..13840))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13603..13622
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13841..13945)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     14013..14031
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg cacggtctcc aggatgatcc agatctgcag gctctgctga aaggttctca
     2641 gctcctcaaa gtcaaaagca gctcctggcg tcgtgaacgc ttctacaaac tgcaggagga
     2701 ctgcaaaacc atctggcagg aatcccgtaa agtaatgcgc tccccagagt ctcagctctt
     2761 cagcattgag gacattcagg aggtacgtat gggtcaccgt accgaaggtc tggagaaatt
     2821 cgctcgcgat atcccagaag atcgctgctt ctctatcgtc ttcaaagacc agcgcaacac
     2881 cctggatctg attgctccat ctccagcaga tgctcagcac tgggttcagg gtctccgcaa
     2941 aatcattcac cactccggtt ctatggacca gcgtcagaaa ggtaccatgg tgagcaaggg
     3001 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     3061 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     3121 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     3181 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     3241 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     3301 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     3361 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     3421 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     3481 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     3541 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     3601 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     3661 ccactccacc ggcggcatgg acgagctgta caagccaagc ttgaattctg caacgtgcat
     3721 gaatggacag gcgcgcattg ctcttccctc caaagtggac ctgtacatca ctcatgtgga
     3781 tcacgtgggt ccgtatctga aggtctacgg ccacgttaac cgagacgccg cctccctgat
     3841 cagcgaacga atcaggaatc tgttgcccac atgctttgcc attgagccca gttggtccgt
     3901 ggagcgccaa caggctctcc tcatacccgg cacgttttgc atattcaaga atatcaatgg
     3961 cccggctcct ggtgatgtgg agtacaggcg gatacgtgtg gtcagtgcag acctggaggg
     4021 tcagtcgatg cgggcggaga tcgatttcgt ggactttggc tataagcgta ctgtagatag
     4081 tcatgattta atgttcccca agcagcccaa gctgcttcag aacattcccc tgcactgctt
     4141 ccagtacatt gtgctgggaa tctgctccga gtgggatcag accgatctgg ctgaggttcg
     4201 gcggctggtt gtcaaccaga tcgttaaaat cactgtggaa cctacacaaa tctgtgacca
     4261 aaagtttgcc agtctgcgct ggaaggattt tgagctcaac gagttcttag tgcaacaaaa
     4321 acaaatcgga gtttcggtcg acaagcaact catgatggac cactgcaaga agctgtggaa
     4381 ggataatccg cagtcgccgg tcaccgaata caacaacaat agtatacaca actcaaagac
     4441 gcccatggag attgcccgtg agcaactagc ggtgcgacaa tccttagctg ctcgcttgga
     4501 tgcacagcga tcggtccaag tgacaccgtc aaggccgcta aacgccgatg cccctgacta
     4561 tacgcctaaa catctgccgc tggtcaatgt gacaaatgta caggtacaaa tgccaggcct
     4621 aaacaatact cagaaaccgg tatttgtatc gacaaatcct tataatcgtg ccaactatca
     4681 gcccgctgtg ccggctgctc aaccatatgt gcccaaggcg aacccccgat cgcaatacac
     4741 ctattacaat gttcgcatga acaaaccgat taacgccatg ccgccgccgg ccggtccaca
     4801 tgtgcccatt cagcacttta atcaacaggc taacaacgtg tcactgagct atgttcctgc
     4861 acggttcact ccacctccaa cgccatcgat tgcgcaacat caaatcccca ttcctgcctt
     4921 cagaaccacc agcttaacgg tgggcctcac ctacgacgtg gttataagct atgtggagaa
     4981 cggtccgtac ctattttggg tgcacctgaa aagcagcgat catgatctga gcaccatgat
     5041 gggtcaaatt gaacggacga agctcaaggc actcgcgcag gctcctgagc ttggaactgc
     5101 ctgtgtggcc cgtttctcag aagatggtca tctgtatcgc gctatggtgt gcgctgttta
     5161 cgcccaacgt taccgcgttg tttacgtgga ctacggaaac tctgagttgt tgtccgcaag
     5221 tgatctgttt caaatacccc cagaactgtt ggaaattaaa ccgtttgctt tccgatttgc
     5281 tttggccgga accaaggaaa ttgagcccat tgacgacagc atgaagcgga ttttcaagaa
     5341 atcagccatt tatcgtaact ttgaactgac tgtccaggcg ccagagagtg tgggctccat
     5401 gcaaacctgc catctcaacc agaatggcac caatatgctg gagcttctta ggcagctaaa
     5461 gaactcacgt cagtcctaca aaaaggcgga gcaacttgaa aacgacgacg ccgtggagat
     5521 tcgttttatt gattcgccca gcaacttcta tgtgcagaaa gtggccaaca taggaaagtt
     5581 cgagcagctc atggacgaga tgttctccta ctacaatgca aaccagagag ttcccgatca
     5641 gttgatcttg ggcgcaccct gcattgttaa atgcgatcag gaatggtacc gtgcagagat
     5701 tctgcgcgtt gatgactctg tgattgtgcg gcatgtagat tttggttatg aacagaatgt
     5761 gaagcggcat ttgattggcc atattgctga gaagcatctg gaaatgcctc gccaggcaat
     5821 taagtgctgc ttgaaaggat tcgagaacag tgaacttagc gaggacaaga ttaccgatca
     5881 gtttgaaatg ttagctgagg aatccaatat ccgaaggcgc acatttagtg tgcgtatctt
     5941 tcgcattgaa cccgatggtc tgaacgtagt aaacctgctg gccaagaacc tgaatgtgat
     6001 gaagaagctc tacaagcttt ctatgccttt tgaacaatat ctgtccctgg aaaagggtca
     6061 gtttaatgca aacaacaccc gtgccgagtc tgtgatctct tctgagttga ataagagcca
     6121 tattttgaac tctacaagca ttggcgagac cgaaaatcgt ttgcaagaac aggaaaaaga
     6181 gcagcaacag aaaaaggtcg atgttaggca gcagcaacta gcagttgaaa ttccgcaagc
     6241 agtaaagtcc gttagcggaa gcaaaaactc gacagactgg gacaaacgta gctccacctc
     6301 ggcaggctca aaggacagta agcgtcagca gcagcaacaa atacagcgaa tcgatcgcca
     6361 tttagacttc agctgtgaga cacagagcac cggtagttac agcagtggca tgagttcgcc
     6421 gcgcaagggt aaccgacaac agaacggtcg cacaccgatc caatcaccac gccataatga
     6481 aaagcaggaa gcgaaaaaaa atgcgcgctt tagcaacagc gagtcacccc gcaggagtcg
     6541 agatggccag caggggaacc aacgctccca aaatgcgcca cagggctatg ctcaaaagcc
     6601 acagcgtcaa aaatccacgt tagacggaaa tatcagctca aagcgttcca gcggagtggg
     6661 gagtgatatt gcctcatcca gctccgaatc ggtggctgcc gccaagccgg agaaatacgt
     6721 ttccttggat aaaccgtatg cgctgcagga gatgaaaaca cctagcaagg aggcagctag
     6781 tttgtcttgg tggctctcgc cattccagtt ctacatcgtg cccaagtctg tctctgcaaa
     6841 gtacgataat ataatgcgtg atatgcgaga gttctaccgc cagaagcaac accagccact
     6901 tcaattaaag gttggatcca ccgtagttgt gcgccagcga aaggacaatg ccattctgcg
     6961 ggctactgtg accgcatgca atcacatgat gcgcaagtat cgcgtttttt gcgtggacac
     7021 aggcagctta ataacggtca cctcggagga tatttggcaa ttggagcagc gctttgcaga
     7081 tccgccctgt atggcacatc gctgcagctt tcacagcgtc gttaccaact atgatccatt
     7141 gtacattgtg gatcgcatgg aaacattcgt tccagttaat gccaaagtcg attgcgagtt
     7201 cgtgtccaaa gagaagagca accaaggctc aaataccagc agcacttgct cctacacagt
     7261 taatattttt gtaaacggag catccctacg agatatgctc gtcaaagcgg aattccttac
     7321 cgaagtggcg cctgagattc gcgttaacct tttagctggt cagcagatta gaggaaaatt
     7381 cacttctatt cgtgacatga caagctttaa ggttcagttt gattatggta acaatgtgaa
     7441 cttcctttgc acctatgacg atgctaaatt tgttaagtcc aatccgaatt tggccaggcg
     7501 atttaaggag ttttacgagg gtaaatcgtt tgccttgaat gtcaagaatg tctgcgaaaa
     7561 caatatcgtt catctgagac ctgtaatgcc gttgttcatg gaggatcgca ggtcattcat
     7621 ctgcccctat cccgtggtgc tgagctcatt ccaagcactc gttgtgtaca ccgctaaacc
     7681 ttaccgcgtg tatgttcaac cgcaggccat agtaccatct atgcagaccc tattggataa
     7741 tatgtatgaa cactacaagg ccaagggcga ctctttgaaa aaatttgatg tgggacagat
     7801 ctgtgctgta cgaagctccg atggcaattg gtacagagcc agaatctcag gaaaggattc
     7861 gaatgcagcg tgcttcgagg tgttttacat cgattacggc aacacggagg aaataaagcg
     7921 tgacgatatc aaggcattgg atgcaaagtt ttatgagcac gcaagcggct ttgcagtgga
     7981 gattaatttg ccgattggcc gacccagcaa cgatacaaag ctaaaggcgc gtatatccga
     8041 gattcttgag gagaaggttg tcactatcaa atcgattgag gtgcggcgca gtcacctaat
     8101 tgccgatgtc attttggaaa acaatcagag tgtgatagac ctgctaaagg ctgagaagct
     8161 tgtacccggc aaagatttgg attacatgcg caagcaaatg gagaaaggaa agtctcgcac
     8221 ttacgagtac attgaaacag tagacctaac tttggacgaa gaggaggata agggtcgcaa
     8281 ggaaactgtc agcaagtcgg gttcggcaaa tgcctcccca aagaaaaaac aacataatga
     8341 caaggatcgc gagcccaaaa agagtaagcc agcagagcct gctcgtactg ttgctccaca
     8401 acctgttgca ttgaaaactc cttcaccagt tccggcggag ccagcaccag tgccaaaacc
     8461 agccactcca gttccagagg ttgttgaagt accagaaatt aatccaactg tcagagaagc
     8521 tgctgccgag tcaaagcaag ctcctgcaca agaggatcct tacaaagatt tggactgcgt
     8581 tgtactgagc cattgcgaca atcctgcaca gttctatgtc cacccgatcg atcagctatc
     8641 gaaactaaat cagctgcatg agaaccttca gattgtgtct ccctctttgc cccagttgat
     8701 gaacgtggta aacggtgcag actgtgtatc gatgtattcg gtggacaaat gctggtatcg
     8761 cgccaaaatc attgatgccg agctaatggt actacttttc atagactatg gcaacacaga
     8821 ttgcgtttcg gatgccactg acatcaaaga aagcatgtgg tcgcatatcg aacccttctg
     8881 cttgccatgc gcactcccga ttcgacccaa gggtactgcg gattgggtgg atgcagcgaa
     8941 tggcatcttt aacgaatcgt actcgaaggt cccgcgtctg gagtacctta cccaaggaga
     9001 tcactacacg accagctatg ttaacatgta tattgatggc gaggatgtgg ctaagaaatt
     9061 aattgccgat ggttttgcta ggccgttaga gtatttggcc agcggatgca gttgctacat
     9121 ttcgcacgtg aatggaattt gtgatttctt tattcaactt gaacgtgatt caaaggcgct
     9181 agaactaatt gagttgtatt tgcgcaagaa ggacacatta aagcccctcg aaggatttga
     9241 aaagggtttg attgtggctg ctctatttga ggacgatgag ctgtggtacc gagcccagtt
     9301 gcagaaggag ttgcccgatt cccgatacga agtactcttt atcgactacg gtaacacctc
     9361 caccacatcg aagtgtctaa tgctgtccga agagattgct agcctaccca gcttatccaa
     9421 gaaatgttcg ctgcagttgc cagatgccta catctcatgg tcaccagaag cggaggccaa
     9481 attcgccgaa ctgacaggcg agggtgagct agtgtttacc acacagcttc tgaagccagg
     9541 acaggatcat gtcaccattg atcttctcct ggatggagag aacatcatcg accgcctgtt
     9601 accgttgtgc cagcgaaagg aacccaagga agccagtaag gagtccttag ctgttaccac
     9661 taaagccatc attacgcacg tggagaatac atcccgtata tatctacagt ttagcgagaa
     9721 ggattcatta atggatatta tatgcgagaa gctaaatgga agcaagctgc agcctaagac
     9781 ggaaaaggct gcagtagatg atatgtgcgt agttcaattc gccgacgact tggagttcta
     9841 tcgctcacgt attctggaag tgctcgaaga tgaccaatac aaagtcattt tgattgatta
     9901 cggcaatacg actgtggttg ataagcttta tgagttacct caagagttca cgctcattaa
     9961 acctgtggcc gaaatatgca gcatggaacc cagcgccata tttgagaaaa ataaagctct
    10021 aaccctgaca acatttgacg cactgctgga cagctgcaag ggtgttgtgg cggtggaatt
    10081 tgtaaacaag tctgccagtc ctcctgtggt cagattgact actaaagata agaggagcct
    10141 gaagatctat gaacaccttc agaagttggt tcaagccgag ctgaaactaa tccaaaagcg
    10201 aaacgagaac tctgaatgca ttatctcata tggaaactca cccaagagtt tttatgtgca
    10261 aatgaagcac aattcagcgg atttggactt gattgtgaag acactgcagt ccttaaaaaa
    10321 ggagaaactg aagaaattaa tcgatcctac tacaaattca aatggtgttt gctactccca
    10381 agaggatgcc tgttactatc gctgtagtat caagtcggta ttggatccta gccagggatt
    10441 tgaagtcttt ttgctagatt atggaaacac cctggtcgtt ccagaggtct ggcagctacc
    10501 ccaagagata gagccgattc catcgttggc tctacactgt cagctgagta agattccaat
    10561 ggatgtgtct gacgaaaagc tagaagaagc ctttgctgct ttgctagagc agcacttcgg
    10621 agaactatac gaaataacta ctcagccgaa cgaggatgag acaaaacctc tgattgctga
    10681 actccgaatc aactacaagg atttcgtcca ggagctggtc agtacggtga ccggcgtaca
    10741 gaaaccgctc gaagcggagc tgcacaactg tgttgtggtg cagttcgatg gccccatgtc
    10801 cttctacgtc cagatggaga gcgatgtgcc agcactagag caaatgactg ataaactgct
    10861 cgatgcagaa caggacctac ccgcctttag cgacctcaaa gagggagctt tgtgtgtggc
    10921 tcagttcccg gaggacgagg tcttctatcg cgcccaaatt cgcaaggttt tggatgacgg
    10981 aaagtgcgag gtgcatttta ttgactttgg caacaatgcc gtgacgcagc agttccgcca
    11041 gctgcctgag gagttggcca agccggcgcg atacagcagg cattgcgagc tggatgcgtc
    11101 gaccatatcc aagtgcgacg cggccctgct ccagtcgttc atcgacactc gcttctctga
    11161 aacattccag gtagagattc tggccaccaa gggaactggc acccatgtcg tacgactctt
    11221 ctaccagagc aagaacatca gcgagaagct tcaggaatgt cagtgagcat gagatatcca
    11281 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
    11341 ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta
    11401 aacccgctga tcagcctcga ctgtgccttc taaggcctga gctcgctgat cagcctcgat
    11461 cgaggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
    11521 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
    11581 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
    11641 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
    11701 tgatcagtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
    11761 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
    11821 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
    11881 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
    11941 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
    12001 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
    12061 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
    12121 aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagccagc
    12181 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    12241 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    12301 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    12361 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
    12421 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    12481 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12541 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12601 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    12661 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    12721 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    12781 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    12841 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    12901 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    12961 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    13021 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    13081 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    13141 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    13201 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    13261 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
    13321 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    13381 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    13441 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    13501 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    13561 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
    13621 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    13681 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    13741 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    13801 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    13861 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    13921 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
    13981 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgt
//
