LOCUS       sequence-401792-       11658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9)
            fused to the KRAB domain as well as a gRNA targeting the tight TRE 
            promoter and the blasticidin resistance gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11658)
  TITLE     CRISPRi plasmids for reduced leakiness of doxycycline-inducible 
            tight TRE promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-dSaCas9-KRAB-gRNA-TRE-blast
FEATURES             Location/Qualifiers
     source          1..11658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..30
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    147..168
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(183..188,189..206,207..213)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    221..237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     226..248
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     245..261
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     245..261
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     280..300
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        282..300
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        328..554
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             555..735
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    782..907
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1400..1633
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1818..1862
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2129..2246
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2269..2572
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2573..2776
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2727..2747
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2804..2830
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2834..2854
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6035..6082
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6116..6310
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             6377..6397
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        6482..6730
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6482..6502
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6653..6672
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6753..6828
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     protein_bind    complement(6840..6864)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6921..6962
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6986..7574
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7039..7059)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7457..7468)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7589..7905
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7756..7891
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7953..8000
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8019..8417
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8504..8737
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8809..8930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8846..8865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8900..8919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8970..9105
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9125..9144)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9126..9144)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9154..9171)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9154..9170)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9163..9185)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9312..9767
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9399..9418)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9609..9630
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9793..9897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9898..9966,9967..10758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10116..10135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10929..11517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11418..11437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
       61 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
      121 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
      181 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
      241 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
      301 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
      361 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
      421 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
      481 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
      541 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
      601 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      661 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      721 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
      781 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
      841 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
      901 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
      961 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     1021 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     1081 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     1141 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     1201 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     1261 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     1321 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     1381 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     1441 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     1501 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     1561 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     1621 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     1681 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     1741 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     1801 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     1861 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     1921 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     1981 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     2041 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     2101 tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca
     2161 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     2221 aacaaattac aaaaattcaa aattttatcg ataagcttgg gagttccgcg ttacataact
     2281 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     2341 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     2401 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     2461 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     2521 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     2581 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     2641 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     2701 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     2761 ctatataagc agagctctct ggctaactac cggtgccacc atgtacccat acgatgttcc
     2821 agattacgct gccccaaaga agaagcggaa ggtcggtatc cacggagtcc cagcagccaa
     2881 gcggaactac atcctgggcc tggccatcgg catcaccagc gtgggctacg gcatcatcga
     2941 ctacgagaca cgggacgtga tcgatgccgg cgtgcggctg ttcaaagagg ccaacgtgga
     3001 aaacaacgag ggcaggcgga gcaagagagg cgccagaagg ctgaagcggc ggaggcggca
     3061 tagaatccag agagtgaaga agctgctgtt cgactacaac ctgctgaccg accacagcga
     3121 gctgagcggc atcaacccct acgaggccag agtgaagggc ctgagccaga agctgagcga
     3181 ggaagagttc tctgccgccc tgctgcacct ggccaagaga agaggcgtgc acaacgtgaa
     3241 cgaggtggaa gaggacaccg gcaacgagct gtccaccaaa gagcagatca gccggaacag
     3301 caaggccctg gaagagaaat acgtggccga actgcagctg gaacggctga agaaagacgg
     3361 cgaagtgcgg ggcagcatca acagattcaa gaccagcgac tacgtgaaag aagccaaaca
     3421 gctgctgaag gtgcagaagg cctaccacca gctggaccag agcttcatcg acacctacat
     3481 cgacctgctg gaaacccggc ggacctacta tgagggacct ggcgagggca gccccttcgg
     3541 ctggaaggac atcaaagaat ggtacgagat gctgatgggc cactgcacct acttccccga
     3601 ggaactgcgg agcgtgaagt acgcctacaa cgccgacctg tacaacgccc tgaacgacct
     3661 gaacaatctc gtgatcacca gggacgagaa cgagaagctg gaatattacg agaagttcca
     3721 gatcatcgag aacgtgttca agcagaagaa gaagcccacc ctgaagcaga tcgccaaaga
     3781 aatcctcgtg aacgaagagg atattaaggg ctacagagtg accagcaccg gcaagcccga
     3841 gttcaccaac ctgaaggtgt accacgacat caaggacatt accgcccgga aagagattat
     3901 tgagaacgcc gagctgctgg atcagattgc caagatcctg accatctacc agagcagcga
     3961 ggacatccag gaagaactga ccaatctgaa ctccgagctg acccaggaag agatcgagca
     4021 gatctctaat ctgaagggct ataccggcac ccacaacctg agcctgaagg ccatcaacct
     4081 gatcctggac gagctgtggc acaccaacga caaccagatc gctatcttca accggctgaa
     4141 gctggtgccc aagaaggtgg acctgtccca gcagaaagag atccccacca ccctggtgga
     4201 cgacttcatc ctgagccccg tcgtgaagag aagcttcatc cagagcatca aagtgatcaa
     4261 cgccatcatc aagaagtacg gcctgcccaa cgacatcatt atcgagctgg cccgcgagaa
     4321 gaactccaag gacgcccaga aaatgatcaa cgagatgcag aagcggaacc ggcagaccaa
     4381 cgagcggatc gaggaaatca tccggaccac cggcaaagag aacgccaagt acctgatcga
     4441 gaagatcaag ctgcacgaca tgcaggaagg caagtgcctg tacagcctgg aagccatccc
     4501 tctggaagat ctgctgaaca accccttcaa ctatgaggtg gaccacatca tccccagaag
     4561 cgtgtccttc gacaacagct tcaacaacaa ggtgctcgtg aagcaggaag aagccagcaa
     4621 gaagggcaac cggaccccat tccagtacct gagcagcagc gacagcaaga tcagctacga
     4681 aaccttcaag aagcacatcc tgaatctggc caagggcaag ggcagaatca gcaagaccaa
     4741 gaaagagtat ctgctggaag aacgggacat caacaggttc tccgtgcaga aagacttcat
     4801 caaccggaac ctggtggata ccagatacgc caccagaggc ctgatgaacc tgctgcggag
     4861 ctacttcaga gtgaacaacc tggacgtgaa agtgaagtcc atcaatggcg gcttcaccag
     4921 ctttctgcgg cggaagtgga agtttaagaa agagcggaac aaggggtaca agcaccacgc
     4981 cgaggacgcc ctgatcattg ccaacgccga tttcatcttc aaagagtgga agaaactgga
     5041 caaggccaaa aaagtgatgg aaaaccagat gttcgaggaa aagcaggccg agagcatgcc
     5101 cgagatcgaa accgagcagg agtacaaaga gatcttcatc accccccacc agatcaagca
     5161 cattaaggac ttcaaggact acaagtacag ccaccgggtg gacaagaagc ctaatagaga
     5221 gctgattaac gacaccctgt actccacccg gaaggacgac aagggcaaca ccctgatcgt
     5281 gaacaatctg aacggcctgt acgacaagga caatgacaag ctgaaaaagc tgatcaacaa
     5341 gagccccgaa aagctgctga tgtaccacca cgacccccag acctaccaga aactgaagct
     5401 gattatggaa cagtacggcg acgagaagaa tcccctgtac aagtactacg aggaaaccgg
     5461 gaactacctg accaagtact ccaaaaagga caacggcccc gtgatcaaga agattaagta
     5521 ttacggcaac aaactgaacg cccatctgga catcaccgac gactacccca acagcagaaa
     5581 caaggtcgtg aagctgtccc tgaagcccta cagattcgac gtgtacctgg acaatggcgt
     5641 gtacaagttc gtgaccgtga agaatctgga tgtgatcaaa aaagaaaact actacgaagt
     5701 gaatagcaag tgctatgagg aagctaagaa gctgaagaag atcagcaacc aggccgagtt
     5761 tatcgcctcc ttctacaaca acgatctgat caagatcaac ggcgagctgt atagagtgat
     5821 cggcgtgaac aacgacctgc tgaaccggat cgaagtgaac atgatcgaca tcacctaccg
     5881 cgagtacctg gaaaacatga acgacaagag gccccccagg atcattaaga caatcgcctc
     5941 caagacccag agcattaaga agtacagcac agacattctg ggcaacctgt atgaagtgaa
     6001 atctaagaag caccctcaga tcatcaaaaa gggcaaaagg ccggcggcca cgaaaaaggc
     6061 cggccaggca aaaaagaaaa agggatccga tgctaagtca ctgactgcct ggtcccggac
     6121 actggtgacc ttcaaggatg tgtttgtgga cttcaccagg gaggagtgga agctgctgga
     6181 cactgctcag cagatcctgt acagaaatgt gatgctggag aactataaga acctggtttc
     6241 cttgggttat cagcttacta agccagatgt gatcctccgg ttggagaagg gagaagagcc
     6301 ctggctggtg gagagagaaa ttcaccaaga gacccatcct gattcagaga ctgcatttga
     6361 aatcaaatca tcagttccga aaaagaaacg caaagtttaa gaattcgccc tttgtacaaa
     6421 aaagcaggct ttaaaggaac caattcagtc gactggatcc ggtaccaagg tcgggcagga
     6481 agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     6541 gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     6601 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     6661 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     6721 acgaaacacc gatcagtgat agagaacgta tggttttagt actctggaaa cagaatctac
     6781 taaaacaagg caaaatgccg tgtttatctc gtcaacttgt tggcgagatt tttttctaga
     6841 cccagctttc ttgtacaaag ttggcattaa agggcgaatt cgttccggag tcgtcgactc
     6901 tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     6961 cgcgtaccgg ttagtaatga tcgacaatca acctctggat tacaaaattt gtgaaagatt
     7021 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     7081 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     7141 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7201 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7261 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7321 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7381 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7441 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7501 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7561 ggccgcctcc ccgcctggcg atggtaccgg tgtggaaagt ccccaggctc cccagcaggc
     7621 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     7681 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7741 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7801 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     7861 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     7921 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
     7981 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagcct ttgtctcaag
     8041 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     8101 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     8161 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     8221 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     8281 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     8341 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     8401 atgtgtggga gggctaagca caattcgagc tcggtacctt taagaccaat gacttacaag
     8461 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     8521 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     8581 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8641 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8701 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     8761 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     8821 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8881 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8941 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     9001 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     9061 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     9121 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     9181 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     9241 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     9301 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     9361 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     9421 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     9481 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     9541 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     9601 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     9661 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     9721 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     9781 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     9841 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     9901 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     9961 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
    10021 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
    10081 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
    10141 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
    10201 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
    10261 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
    10321 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
    10381 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
    10441 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
    10501 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
    10561 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
    10621 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
    10681 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
    10741 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
    10801 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
    10861 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    10921 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    10981 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    11041 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    11101 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    11161 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    11221 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    11281 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    11341 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    11401 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    11461 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    11521 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    11581 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    11641 accgctcgcc gcagccga
//
