LOCUS       sequence-401793-        8520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of Flag-Tagged human HNRNPA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8520)
  TITLE     Alarcon lab plasmids
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label:  pLenti-C-Myc-DDK-IRES-Puro HNRNPA1
FEATURES             Location/Qualifiers
     source          1..8520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(77..736)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     651..670
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(737..841)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(916..935)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1106..1485
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1487..1685
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1703..1883
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1930..2055
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2552..2785
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2970..3014
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3312..3429
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3512..3891
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3892..4095
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4121..4140
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4121..4139
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4985..4996
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             5175..5204
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5223..5246
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5280..5853
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5303..5855
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5447..5464)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5674..5693
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5855..6454
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5855..5874)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6351..6371
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6484..6517)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6573..7161
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6626..6646)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7044..7055)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7164..7180)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7165..7181)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7690..7870
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7932..8520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8012..8031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgattac gccccgccct gccactcatc gcagtactgt tgtaattcat
      121 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
      181 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
      241 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
      301 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
      361 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
      421 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
      481 cccatatcac cagctcaccg tctttcattg ccatacggaa ctccggatga gcattcatca
      541 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
      601 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
      661 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
      721 tgattttttt ctccatactc ttcctttttc aatattattg aagcatttat cagggttatt
      781 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
      841 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
      901 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
      961 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     1021 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     1081 cgatgtacgg gccagatatc gcgttgacat tgattattga ctagttatta atagtaatca
     1141 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     1201 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     1261 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     1321 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     1381 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     1441 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     1501 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     1561 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     1621 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     1681 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     1741 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     1801 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     1861 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     1921 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     1981 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     2041 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     2101 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     2161 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     2221 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     2281 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     2341 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     2401 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     2461 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     2521 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     2581 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     2641 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     2701 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     2761 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     2821 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     2881 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     2941 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     3001 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     3061 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     3121 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     3181 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     3241 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     3301 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3361 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3421 caaaattttc gggtttatta cagggacagc agagatccag tttggttagt accgggcccg
     3481 ctctagacat gtccaatatg accgccatgt tgacattgat tattgactag ttattaatag
     3541 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     3601 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     3661 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     3721 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct
     3781 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg
     3841 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     3901 ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     3961 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     4021 tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     4081 tatataagca gagctcgttt agtgaaccgt cagaattttg taatacgact cactataggg
     4141 cggccgggaa ttcgtcgact ggatccggta ccgaggagat ctgccgccgc gatcgcatgt
     4201 ctaagtcaga gtctcctaaa gagcccgaac agctgaggaa gctcttcatt ggagggttga
     4261 gctttgaaac aactgatgag agcctgagga gccattttga gcaatgggga acgctcacgg
     4321 actgtgtggt aatgagagat ccaaacacca agcgctccag gggctttggg tttgtcacat
     4381 atgccactgt ggaggaggtg gatgcagcta tgaatgcaag gccacacaag gtggatggaa
     4441 gagttgtgga accaaagaga gctgtctcca gagaagattc tcaaagacca ggtgcccact
     4501 taactgtgaa aaagatattt gttggtggca ttaaagaaga cactgaagaa catcacctaa
     4561 gagattattt tgaacagtat ggaaaaattg aagtgattga aatcatgact gaccgaggca
     4621 gtggcaagaa aaggggcttt gcctttgtaa cctttgacga ccatgactcc gtggataaga
     4681 ttgtcattca gaaataccat actgtgaatg gccacaactg tgaagttaga aaagccctgt
     4741 caaagcaaga gatggctagt gcttcatcca gccaaagagg tcgaagtggt tctggaaact
     4801 ttggtggtgg tcgtggaggt ggtttcggtg ggaatgacaa cttcggtcgt ggaggaaact
     4861 tcagtggtcg tggtggcttt ggtggcagcc gtggtggtgg tggatatggt ggcagtgggg
     4921 atggctataa tggatttggt aatgatggaa gcaattttgg aggtggtgga agctacaatg
     4981 attttgggaa ttacaacaat cagtcttcaa attttggacc catgaaggga ggaaattttg
     5041 gaggcagaag ctctggcccc tatggcggtg gaggccaata ctttgcaaaa ccacgaaacc
     5101 aaggtggcta tggcggttcc agcagcagca gtagctatgg cagtggcaga agatttacgc
     5161 gtacgcggcc gctcgagcag aaactcatct cagaagagga tctggcagca aatgatatcc
     5221 tggattacaa ggatgacgac gataaggttt aaacctaggc gtagcggccg caaattccgc
     5281 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     5341 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     5401 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     5461 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     5521 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     5581 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     5641 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     5701 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     5761 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     5821 gacgtggttt tcctttgaaa aacacgatga taatatgacc gagtacaagc ccacggtgcg
     5881 cctcgccacc cgcgacgacg tcccccgggc agtacgcacc ctcgccgccg cgttcgccga
     5941 ctaccccgcc acgcgccaca ccgtcgatcc agaccgccac atcgagcggg tcaccgagct
     6001 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6061 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     6121 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     6181 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     6241 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     6301 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     6361 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     6421 cacctggtgc atgacccgca agcccggtgc ctgatgtaca agtaggattc gtcgagggac
     6481 ctaataactt cgtatagcat acattatacg aagttataca tgtttaaggg ttccggttcc
     6541 actaggtaca attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     6601 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6661 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6721 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6781 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6841 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6901 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6961 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     7021 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     7081 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     7141 ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagacct agaaaaacat
     7201 ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca
     7261 caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact
     7321 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7381 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     7441 ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga
     7501 tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag
     7561 aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta
     7621 ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg
     7681 gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7741 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7801 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7861 atctctagca gcatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7921 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7981 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8041 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8101 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8161 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8221 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8281 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8341 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8401 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8461 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
//
